Antioxidant, antimicrobial and cytotoxic potential of condensed tannins from Leucaena leucocephala hybrid-Rendang

作     者：Mazni Abu Zarin Ho Yin Wan Azizul Isha Nurdin Armania 

作者机构：Laboratory of Vaccines and ImmunotherapeuticsInstitute of BioscienceUniversiti Putra Malaysia43400 UPM SerdangSelangorMalaysia Laboratory of Natural ProductsInstitute of BioscienceUniversiti Putra Malaysia43400 UPM SerdangSelangorMalaysia Department of Biomedical ScienceFaculty of Medicine and Health SciencesUniversiti Putra Malaysia43400 UPM SerdangSelangorMalaysia 

出 版 物：《Food Science and Human Wellness》 (食品科学与人类健康（英文）)

年 卷 期：2016年第5卷第2期

页      面：65-75页

学科分类：1007[医学-药学(可授医学、理学学位)] 10[医学] 

基　　金：This study was financially supported by the Universiti Putra Malaysia(RUGS:9199879).Special thanks to one of the author the late Dr Sieo Chin Chin 

主　　题：Antioxidant Anti-microbial Condensed tannins Cytotoxic Leucaena leucocephala 

摘      要：Condensed tannins(CTs)are one of the promising compounds due to their potentially health-promoting *** this study,CTs were extracted from a Leucaena leucocephala hybrid-Rendang and subjected to various biological studies including antioxidant(using Ferric reducing antioxidant power(FRAP),DPPH and ABTS radical scavenging assay),anti-microbial(against different pathogens)and cytotoxic activities(toward human breast adenocarcinoma(MCF-7),human colon carcinoma(HT29),human cervical carcinoma(HeLa)and human liver carcinoma(HepG2)cell lines)in cancer cells through in vitro *** structural characteristics and purity of CTs extract were determined using 13C *** results showed that CTs exhibited higher in vitro antioxidant activities(2257.12±80.55 mg TEAC/g extract,605.3±1.82 mg TEAC/g extract and 1014.03±1.20 mg TEAC/g extract in FRAP,ABTS and DPPH assay,respectively)and demonstrated anti-microbial activities toward selected Gram’s positive and Gram’s negative bacteria tested with MIC and MBC value at 6.25–50 mg/***,among other selected cancer cells,CTs also demonstrated cytotoxic activity toward human breast cancer cells(MCF-7)(IC50=38.33±2.08mg/mL).Characteristic of apoptosis such as cell shrinkage,nuclear condensation and apoptotic bodies were shown in *** preliminary investigations have provided scientific rationale to use CTs as an alternative therapy for various oxidative and inflammatory associated diseases.©2016 Beijing Academy of Food *** and hosting by Elsevier *** rights reserved.