检索结果-南通市图书馆

Two homologous INDOLE-3-ACETAIVHDE (IAM) HYDROLASE genes are required for the auxin effects of IAM in Arabidopsis

在线全文

学校读者我要写书评

暂无评论

Journal of Genetics and Genomics 2020年第3期47卷 157-165页

作者： Yangbin Gao Xinhua Dai Yuki Aoi Yumiko Takebayashi Liping Yang Xiaorui Guo Qiwei Zeng Hanchuanzhi Yu hiroyuki kasahara Yunde Zhao Section of Cell and Developmental Biology University of California San DiegoLa JollaCA92093-0116USA RIKEN Center for Sustainable Resource Science Kanagawa230-0045Japan School of Life Sciences Jilin Normal UniversitySiping136000China Key Laboratory of Plant Ecology Northeast Forestry UniversityHarbin150040China State Key Laboratory of Silkworm Genome Biology Institute of Sericulture and Systems BiologySouthwest UniversityChongqing400716China Institute of Global Innovation Research Tokyo University of Agriculture and TechnologyTokyo183-8509Japan Department of Bioregulation and Biointeraction Graduate School of AgricultureTokyo University of Agriculture and TechnologyFuchu183-8509Japan

Indole-3-acetamide(IAM)is the first confirmed auxin biosynthetic intermediate in some plant pathogenic bacteria.Exogenously applied IAM or production of IAM by overexpressing the bacterial iaaM gene in Arabidopsis causes auxin overproduction phenotypes.However,it is still inconclusive whether plants use IAM as a key precursor for auxin biosynthesis.Herein,we reported the isolation IAM HYDROLASE 1(IAMH1)gene in Arabidopsis from a forward genetic screen for IAM-insensitive mutants that display normal auxin sensitivities.IAMH1 has a close homolog named IAMH2 that is located right next to IAMH1on chromosomeⅣin Arabidopsis.We generated iamh1 iamh2 double mutants using our CRISPR/Cas9gene editing technology.We showed that disruption of the IAMH genes rendered Arabidopsis plants resistant to IAM treatments and also suppressed the iaaM overexpression phenotypes,suggesting that IAMH1 and IAMH2 are the main enzymes responsible for converting IAM into indole-3-acetic acid(IAA)in Arabidopsis.The iamh double mutants did not display obvious developmental defects,indicating that IAM does not play a major role in auxin biosynthesis under normal growth conditions.Our findings provide a solid foundation for clarifying the roles of IAM in auxin biosynthesis and plant development.

关键词： Auxin Auxin biosynthesis Indole-3-acetamide Arabidopsis CRISPR IAMH1 IAMH2

Novel Approaches for Inhibitor Screening and Target Identification using Arabidopsis Full-Length cDNA Overexpression （FOX） Lines

在线全文

学校读者我要写书评

暂无评论

Novel Approaches for Inhibitor Screening and Target Identifi...

Early relaparotomy following pediatric living-donor liver transplantation:experience in an Indonesian national referral hospital

The 1st International Conference on Plant Secondary Metabolism

作者： hiroyuki kasahara Takanari Ichikawa Minami Matsui Yuji Kamiya RIKEN Plant Science CenterYokohama Japan RIKEN Plant Science Center Yokohama Japan

＜正＞Organisms have two possible routes for the biosynthesis of isoprenoids;the mevalonate （MVA） and methylerythritol phosphate （MEP） pathways.The MEP pathway has been recently recognized as important target of drug and agrochemical developments and chemical genetics studies,e.***.fosmidomycin shows antima- larial activity by inhibition of 1-deoxyxylulose-5-phosphate reductoisomerase （DXR）,and clomazone has been used for herbicide,which metabolite ketoclomazone inhibits 1-deoxyxylulose-5-phosphate synthase.Plants have

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

World Journal of Pediatric Surgery 2023年第1期6卷 42-47页

作者： Tri Hening Rahayatri Alif Rizqy Soeratman Rusdah Binti Muhammad Amin Dalia Benchamas Margiadi Riana Pauline Tamba Mureo kasahara Division of Pediatric Surgery Department of SurgeryRumah Sakit Umum Pusat Nasional Dr Cipto MangunkusumoCentral JakartaIndonesia Division of Pediatric Surgery Department of SurgeryUniversitas Indonesia Fakultas KedokteranCentral JakartaIndonesia Department of Surgery Rumah Sakit Umum Pusat Nasional Dr Cipto MangunkusumoCentral JakartaIndonesia Rumah Sakit Umum Pusat Nasional Dr Cipto Mangunkusumo Central JakartaIndonesia Department of Transplantation National Center for Child Health and DevelopmentSetagaya-kuJapan

Background Living donor liver transplantation(LDLT)remains the only curative treatment for children with end-stage liver disease;however,complications of the procedure are associated with indications for early relaparotomy.Several risk factors associated with early relaparotomy after liver transplantation include pediatric end-stage liver disease(PELD)score,warm ischemia time(WIT),and cold ischemia time(CIT).Our study investigated the incidence and indications of early relaparotomy in postoperative pediatric LDLT recipients and compared the outcomes with patients who did not require relaparotomy.Methods A retrospective cohort study of pediatric LDLT recipients from Cipto Mangunkusumo Hospital,Jakarta,Indonesia,was collected from 2010 to August 2022.Indications for early relaparotomy were investigated.Factors analyzed in the early relaparotomy group compared with the nonrelaparotomy group included intraoperative blood loss,surgery duration,CIT,WIT,and PELD score.Results The highest indication for early relaparotomy was biliary leakage.Most patients who underwent early relaparotomy only had one incidence of relaparotomy(60%).The surgery duration in subjects with early relaparotomy was longer by a median of 3 hours compared with those without early relaparotomy(p=0.289).Intraoperative blood loss was greater in early relaparotomy subjects than in subjects without early relaparotomy(95 vs 77mL/kg,p=0.552).Other factors,such as PELD score,CIT,and WIT,also showed no significant difference between the two groups.Conclusion Biliary leakage was the most common indication for early relaparotomy in our center.There were no preoperative or intraoperative factors that significantly influenced the incidence of early relaparotomy due to the limited sample size and the early advancement of our liver transplant center.

关键词： laparotomy pediatric donor

儿童肝移植:改善长期预后的研究进展及未来研究方向

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science Bulletin 2022年第19期67卷 1929-1931页

作者：冯浩奚志峰 Mureo kasahara 夏强 Department of Liver Surgery Renji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200127China Shanghai Engineering Research Centre of Transplantation and Immunology Shanghai 200127China Shanghai Institute of Transplantation Shanghai 200127China Transplantation Center National Center for Child Health and DevelopmentTokyo 157-8535Japan

Liver transplantation(LT)is a lifesaving procedure for children with end-stage liver disease,inoperable malignancies,and certain inherited metabolic disorders.The first successful pediatric LT(PLT)procedure was performed in 1967 by Thomas Starzl for a child with unresectable hepatoblastoma.During the last five decades,LT techniques have matured,and the procedure has undergone radical changes.Along with the improvements in antiinfective agents and immunosuppression(IS),the PLT community’s expectations regarding allograft and patient survival have shifted from years to decades.However,developing lifelong post-transplant care strategies remains a considerable challenge because optimal outcomes require a continuous balance between allograft health and patient health.

关键词： Liver shifted radical

The Role and the Function of Japan Institute of Sports Sciences and National Training Center in Japan

在线全文

学校读者我要写书评

暂无评论

中国体育科技 2009年第1期45卷 30-32页

作者： Kazuya kasahara Japan Institute of Sports Sciences Japan

Gold Medals in Summer Qlympics Measures A 1) Program to develop junior athletes to top level 2) National Training Center 3) National Coach Academy B 1) Sports medicine and sciences(JISS) 2) Anti-doping 3) To hold inte... 详细信息

关键词： The Role and the Function of Japan Institute of Sports Sciences and National Training Center in Japan

Unsupervised calibration for noninvasive glucose-monitoring devices using mid-infrared spectroscopy

在线全文

学校读者我要写书评

暂无评论

Journal of Innovative Optical Health Sciences 2018年第6期11卷 87-96页

作者： Ryosuke kasahara Saiko Kino Shunsuke Soyama Yuji Matsuura Ricoh Institute of Information and Communication Technology Research and Development Division Ricoh Company 2-7-1 IzumiEbina 243-0460Japan Graduate School of Engineering Tohoku University 6-6-05 AobaSendai 980-8579Japan Graduate School of Biomedical Engineering Tohoku University 6-6-05 AobaSendai 980-8579Japan

Noninvasive,glucose-monitoring technologies using infrared spectroscopy that have been studied typically require a calibration process that involves blood collection,which renders the methods somewhat invasive.We develop a truly noninvasive,glucose-monitoring technique using midinfrared spectroscopy that does not require blood collection for calibration by applying domain adaptation(DA)using deep neural networks to train a model that associates blood glucose concentration with mid-infrared spectral data without requiring a training dataset labeled with invasive blood sample measurements.For realizing DA,the distribution of unlabeled spectral data for calibration is considered through adversarial update during training networks for regression to blood glucose concentration.This calibration improved the correlation coeffcient between the true blood glucose concentrations and predicted blood glucose concentrations from 0.38 to 0.47.The result indicates that this calibration technique improves prediction accuracy for mid-infrared glucose measurements without any invasively acquired data.

关键词： Noninvasive glucose monitoring calibration mid-infrared spectroscopy domain adaptation neural network

Comparison of corneal biomechanical properties in normal tension glaucoma patients with different visual field progression speed

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International Journal of Ophthalmology(English edition) 2016年第7期9卷 973-978页

作者： Ying Hong Nobuyuki Shoji Tetsuya Morita Kazunori Hirasawa Kazuhiro Matsumura Masayuki kasahara Kimiya Shimizu Department of Ophthalmology Peking University Third Hospital Key Laboratory of Vision Loss and RestorationMinistry of Education Beijing 100191 China Department of Rehabilitation Orthoptics and Visual Science Course Kitasato University School of Allied Health Sciences Sagamihara City 252-0373 Japan Department of Ophthalmology Kitasato University Hospital Sagamihara City 252-0373 Japan Kitasato University Sagamihara City 252-0373 Japan

AIM：To compare the corneal biomechanical properties difference by ocular response analyzer（ORA） in normal tension glaucoma（NTG） patients with different visual field（VF） progression speed. METHODS：NTG patients with well-controlled Goldmann applanation tonometer（GAT） who routinely consulted Kitasato University Hospital Glaucoma Department between January 2010 and February 2014 were enrolled.GAT and ORA parameters including corneal compensated intraocular pressure（lOPcc）,Goldmann estimated intraocular pressure（lOPg）,corneal hysteresis（CH）,corneal resistance factor（CRF） were recorded.VF was tested by Swedish interactive threshold algorithm（SITA）-standard 30-2 fields.All patients underwent VF measurement regularly and GAT did not exceed 15 mm Hg at any time during the 3y follow up.Patients were divided into four groups according to VF change over 3y,and ORA findings were compared between the upper 25th percentile group（slow progression group） and the lower 25th percentile group（rapid progression group）.RESULTS：Eighty-two eyes of 56 patients were studied.There were 21 eyes（21 patients） each in rapid and slow progression groups respectively.GAT,lOPcc,lOPg,CH,CRF were 12.1＋1.4 mm Hg,15.8±1.8 mm Hg,12.8±2.0 mm Hg,8.4±1.1 mm Hg,7.9±1.3 mm Hg respectively in rapid progression group and 11.5±1.3 mm Hg,13.5±2.1 mm Hg,11.2±1.6 mm Hg,9.3±1.1 mm Hg,8.2±0.9 mm Hg respectively in slow progression group（P=0.214,〈0.001,0.007,0.017,0.413,respectively）.In bivariate correlation analysis,lOPcc,lOPcc-GAT and CH were significant correlated with m△MD（r =-0.292,-0.312,0.228 respectively,P =0.008,0.004,0.039 respectively）.CONCLUSION：Relatively rapid VF progression occurred in NTG patients whose lOPcc are rather high,CH are rather low and the difference between lOPcc and GAT are relatively large.Higher lOPcc and lower CH are associated with VF progression in NTG patients.This study suggests that GAT measures might underestimate the IOP in such patients.

关键词： ocular response analyzer intraocular pressure corneal biochemical properties visual field normal tension glaucoma

Preparation of Laminin-apatite-polymer Composites Using Metastable Calcium Phosphate Solutions

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Wuhan University of Technology(Materials Science) 2005年第B12期20卷 220-222页

作者： A Oyane M kasahara N Ichinose Y Yokoyama M Uchida A Ito Nanotechnology Research Institute National Institute of Advanced Industrial Science and Technology (AIST)Central 5 1-1-1 Higashi Tsukuba 305-8565 Japan School of Science and Engineering Waseda University 3-4-1 Okubo Shinjuku 169-8555Japan Institute for Human Science and Biomedical Engineering National Institute of Advanced Industrial Science and Technology (AIST)Central 6 1-1-1 Higashi Tsukuba 305-8566 Japan

A synthetic polymer with a laminin-apatite composite layer on its surface would be useful as a percutaneous device. The preparation of such a composite was attempted in the present study using poly（ ethylene terephthalate ）（PET） and polyethylene （PE） as the synthetic polymer. PET and PE plates and those pretreated with an oxygen plasma were alternately dipped in calcium and phosphate ion solutions, and then immersed in a metastable calcium phosphate solution supplemented with laminin （ LCP solution ）. The PET and PE plates pretreated with an oxygen plasma formed a uniform and continuous layer of a laminin-apatite composite on their surfaces. In contrast, the PET and PE plates that had not been pretreated with an oxygen plasma did not form a continuous layer of a laminin-apatite composite on their surfaces. The hydrophilic functional groups on the PET and PE surfaces introduced by the plasma treatment were responsible for the successful laminin-apatite coruposite coating.

关键词： apatite laminin composite polymer percutaneous device calcium phosphate coating

Nitrogen removal efficiencies and microbial communities in full-scale IFAS and MBBR municipal wastewater treatment plants at high COD:N ratio

在线全文

学校读者我要写书评

暂无评论

Frontiers of Environmental Science & Engineering 2020年第6期14卷 251-263页

作者： Supaporn Phanwilai Naluporn Kangwannarakul Pongsak(Lek)Noophan Tamao kasahara Akihiko Terada Junko Munakata-Marr Linda Ann Figueroa Department of Environmental Enginering Faculty of EngneringKasetsart UnivrsityBangkok 10900Thailand Laboratory of Ecohydrology Division of Forest SciencesDepartment of Agro-enironmental SciencesKyushu UniversityFukuoka 819-0395Japan Deparment of Chemicl Enineing and Instite of Global Imovain Rescarch Tokyo University of Agrcature&TechnoloTokyo 184-8588Japan Depatmtent of Civil and Enivonmenal Enincrn Colordo School of Mines Goldeco 80401USA

The relative locations of AOB,NOB,and DNB were examined for three different kinds of carriers in two types of hybrid bioflm process configurations:integrated fixed-film activated sludge(IFAS)and moving bed bioflm reactor(MBBR)processes.IFAS water resource recovery facilities(WRRFs)used Anodkainess^TM K1 carriers(KC)at Broomfield,Colorado,USA and polypropylene resin crriers(R)at Fukuok,Japa,whic MBBR WRFS used KC crier at South Adams Couty,Colorado,USA and sponge carriers(SC)at Saga,Japan.Influent COD to N ratios ranged from 8:1 to 15:1.The COD and BOD removal efficiencies were high(96%-98%);NH^+4-N and TN removal efficiencies were more varied at 72%98%and 64%-77%,respectively.The extent of TN removal was higher at high SRT,high COD:N ratio and low DO concentration in the anoxic tank.In IFAS,RC with high specific surface area.(SSA)maintained higher AOB population than KC.Sponge carriers with high SSA maintained higher overall bacteria population than KC in MBBR systems.However,the DNB were not more abundant in high SSA crriers.The diversity of AOB,NOB,and DNB was fairly similar in different carriers.Nitrosomonas sp.dominated over Nitrosospira sp.while denitrifying bacteria included Rhodobacter sp.,Sulfuritalea sp.,.Rubrivivax sp:Paracoccus sp..and Pseudomonas sp.The results from this work suggest that high SRT,high.COD:N ratio,low DO concentration in anoxic tanks,and carriers with greater surface area may be recommended for high COD,BOD and TN removal in WRRFs with IFAS and MBBR systerms.

关键词： IFAS MBBR Anokaness^TM K1 carrier Polypropylene resin carrier Sponge carrier