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A preliminary study on rectal dose reduction associated with hyaluronic acid implantation in brachytherapy for prostate cancer

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Asian Journal of Urology 2024年第2期11卷 286-293页

作者： Tairo Kashihara Yuka Urago Hiroyuki Okamoto Mihiro Takemori Hiroki Nakayama Shohei Mikasa Tetsu Nakaichi Kotaro Iijima Takahito Chiba Junichi Kuwahara Satoshi Nakamura Weishan Chang Yoshiyuki Matsui hiroshi Igaki Department of Radiation Oncology National Cancer Center Hospital5-1-1 TsukijiChuo-kuTokyoJapan Radiation Safety and Quality Assurance Division National Cancer Center Hospital5-1-1 TsukijiChuo-kuTokyoJapan Department of Radiological Sciences Graduate School of Human Health SciencesTokyo Metropolitan University7-2-10 Higashi-OguArakawa-kuTokyoJapan Department of Radiological Technology National Cancer Center Hospital5-1-1 TsukijiChuo-kuTokyoJapan Department of Urological Oncology National Cancer Center Hospital5-1-1 TsukijiChuo-kuTokyoJapan

Objectives: Hydrogel spacer (HS) was developed to reduce rectal toxicities caused by radiotherapy, but has been reported to cause major adverse events. Our institute has attempted to introduce a hyaluronic acid (HA) as an alternative spacer. This study aimed to compare rectal doses and geometric distributions between the HS and HA implantation in prostate ***: HS and HA were inserted in 20 and 18 patients undergoing high-dose brachytherapy, respectively. The rectum spacer volumes injected were 10 mL and 22 mL, respectively. In the treatment planning system, 13.5 Gy was administered with common catheter positions. The rectal dose indices were assessed between the spacer groups for dosimetry evaluation. Distances between the prostate and rectum and configurations of the spacers were ***: The mean doses irradiated to 0.1 and 2 mL of the rectum were 10.45 Gy and 6.71 Gy for HS, and 6.73 Gy and 4.90 Gy for HA (p<0.001). The mean minimum distances between the prostate and rectum were 1.23 cm and 1.79 cm for HS and HA, respectively (p<0.05). Geometrical configuration comparisons revealed that HA has a higher ability to expand the space than ***: The rectal dose reduction ability of HA is significantly greater than that of HS, suggesting its potential as a new spacer.

关键词： Brachytherapy Hyaluronicacid Hydrogel Prostaticneoplasm Radiotherapy

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Study on Distribution of Four Pseudomonas Species in Living Environment Using Multiplex PCR

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Open Journal of Stomatology 2024年第2期14卷 77-86页

作者： Sachiyo Hayashi Koji Umezawa Osamu Tsuzukibashi Akira Fukatsu Mana Fuchigami Chiaki Komine hiroshi Yamamoto Mio Hagiwara-Hamano Yukiko Iizuka Satoshi Uchibori Masanobu Wakami hiroshi Murakami Taira Kobayashi Masahiko Fukumoto Takato Nomoto Department of Special Needs Dentistry Nihon University School of Dentistry at Matsudo Chiba Japan Department of Laboratory Medicine for Dentistry for the Compromised Patient Nihon University School of Dentistry at Matsudo Chiba Japan Department of Oral Surgery Nihon University School of Dentistry at Matsudo Chiba Japan Department of Fixed Prosthodontics Nihon University School of Dentistry at Matsudo Chiba Japan Department of Oral Implantology Nihon University School of Dentistry at Matsudo Chiba Japan

Purpose: The genus Pseudomonas is a ubiquitous microorganism frequently detected from immunocompromised patients. The inherent resistance to numerous antimicrobial agents contributes to the opportunistic character of this pathogen exhaustive monitoring of this pathogen is considered of critical importance to public health organizations. The reliable identification method able to distinguish genetic close Pseudomonas species is needed, because these organisms are difficult to differentiate by phenotypic or biochemical methods. The purpose of the present study was to design species-specific primers in order to identify and detect four Pseudomonas species which are frequently detected from the human oral cavities, and to investigate the distribution of these organisms in the living environment using a multiplex PCR. Methods: Polymerase chain reaction (PCR) primers were designed based on partial sequences of the rpoD gene of four Pseudomonas species. Swab samples were collected from fifty washstands, and the distribution of Pseudomonas species was investigated using a conventional PCR at genus level and a multiplex PCR at species level. Results: Multiplex PCR method developed in this study was able to distinguish four Pseudomonas species clearly. The genus Pseudomonas was detected from all samples (100%), whereas P. putida, P, aeruginosa, P. stutzeri and P. fluorescens were detected at 44%, 8%, 4% and 2% in fifty swab samples, respectively. Conclusion: Our developed one-step multiplex PCR method is accurate, specific, cost-effective, time-saving, and works without requiring DNA extraction. It was indicated that washstands were the uninhabitable environment for P. putida, P, aeruginosa, P. stutzeri and P. fluorescens.

关键词： Genus Pseudomonas Multiplex PCR Living Environment

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Importance of adequate immunosuppressive therapy for the recovery of patients with "life-threatening" severe exacerbation of chronic hepatitis B

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World Journal of Gastroenterology 2005年第8期11卷 1109-1114页

作者： Keiichi Fujiwara Osamu Yokosuka hiroshige kojima Tatsuo Kanda Hiromitsu Saisho Hiroyuki Hirasawa hiroshi Suzuki Department of Medicine and Clinical Oncology Department of Emergency and Critical Care Medicine Department of Surgery

AIM: Hepatitis B virus (HBV) re-activation often occurs spontaneously or after withdrawal of immunosuppressive therapy in patients with chronic hepatitis B. Severe exacerbation, sometimes developing into fulminant hepatic failure, is at high risk of mortality. The efficacy of corticosteroid therapy in 'clinically severe' exacerbation of chronic hepatitis B has not been well demonstrated. In this study we evaluated the efficacy of early introduction of high-dose corticosteroid therapy in patients with life-threatening severe exacerbation of chronic hepatitis B. METHODS: Twenty-two patients, 14 men and 8 women, were defined as 'severe' exacerbation of chronic hepatitis B using uniform criteria and enrolled in this study. Eleven patients were treated with corticosteroids at 60 mg or more daily with or without anti-viral drugs within 10 d after the diagnosis of severe disease ('early high-dose' group) and 11 patients were either treated more than 10 d or untreated with corticosteroids ('non-early high-dose' group). RESULTS: Mean age, male-to-female ratio, mean prothrombin time (FT) activity, alanine transaminase (ALT) level, total bilirubin level, positivity of HBeAg, mean IgM-HBc titer, and mean HBV DNA polymerase activity did not differ between the two groups. Ten of 11 patients of the 'early high-dose' group survived, while only 2 of 11 patients of the 'non-early high-dose' group survived (P<0.001). During the first 2 wk after the introduction of corticosteroids, improvements in PT activities and total bilirubin levels were observed in the 'early high-dose' group. Both ALT levels and HBV DNA polymerase levels fell in both groups. CONCLUSION: The introduction of high-dose corticosteroid can reverse deterioration in patients with 'clinically life-threatening' severe exacerbation of chronic hepatitis B , when used in the early stage of illness.

关键词： Chronic hepatitis B Severe exacerbation Immunosuppressive therapy

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Lysosomal Enzyme Activities in Cultured Retinal Pigment Epithelial and Glial Cells of RCS Rat

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Eye Science 1996年第1期16卷 20-27页

作者： Hiroaki Naka hiroshi Kuriyama Shinsuke kojima Shunji Tsuboi Itami Research Laboratory Senju pharmaceutical Co.Ltd.1-1SakuragaokaOshikaItami 664JapanItami Research LaboratorySenju pharmaceutical Co.Ltd.1-1SakuragaokaOshikaItami 664JapanInstitute of OphthalmologyTane Memorial Eye Hospital1-1-39 SakaigawaNishi-KuOsaka 550JapanInstitute of OphthalmologyTane Memorial Eye Hospital1-1-39 SakaigawaNishi-KuOsaka 550Japan

Purpose: To compare the activities of acid phosphatase, N-acetyl-β-glu-cosaminidase and a- mannosidase in cultured retinal pigment epithelium (RPE)and glial cells of Royal College of Surgeons (RCS) rat with those in Long Evans(LE).Methods: The cultured RPE and glial cells of RCS and LE rat were plated into thesame 96 well microtitre, and the biochemical method in microsystem were usedfor enzyme ***: The activities of acid phosphatase and N-acetyl- β-glucosaminidase arehigher by, respectively, 30% and 46% in cultured RPE of RCS rat than LE *** activity of a- mannosidase has no significant difference. The activities of 3enzymes in the retinal glial cells derived from RCS rats are higher than LE rat by43% to 77%.Conclusion: These results suggest that the high activities of lysosomal enzymes inRCS RPE and glial cells may play an important role in the pathogenesis of retinaldystrophy. Eye Science 1996; 12:20-27.

关键词： RCS rat retinal pigment epithelium glial cell lysosomal enzyme cell culture

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High-grade pancreatic intraepithelial neoplasia diagnosed based on changes in magnetic resonance cholangiopancreatography findings:A case report

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World Journal of Clinical Cases 2024年第8期12卷 1487-1496页

作者： Nao Furuya Atsushi Yamaguchi Naohiro Kato Syuhei Sugata Takuro Hamada Takeshi Mizumoto Yuzuru Tamaru Ryusaku Kusunoki Toshio Kuwai Hirotaka Kouno Kazuya Kuraoka Yoshiyuki Shibata Sho Tazuma Takeshi Sudo hiroshi Kohno Shiro Oka Department of Gastroenterology National Hospital Organization Kure Medical Center and Chugoku Cancer CenterKure 737-0023HiroshimaJapan Department of Gastroenterology Hiroshima University HospitalHiroshima 734-8551HiroshimaJapan Department of Pathology National Hospital Organization Kure Medical Center and Chugoku Cancer CenterKure 737-0023HiroshimaJapan Department of Surgery National Hospital Organization Kure Medical Center and Chugoku Cancer CenterKure 737-0023HiroshimaJapan

BACKGROUND High-grade pancreatic intraepithelial neoplasia(PanIN)exhibits no mass and is not detected by any examination ***,it can be diagnosed by pancreatic juice cytology from indirect *** previous cases were diagnosed based on findings of a focal stricture of the main pancreatic duct(MPD)and caudal MPD dilatation and subsequent pancreatic juice cytology using endoscopic retrograde cholangiopancreatography(ERCP).We experienced a case of high-grade PanIN with an unclear MPD over a 20-mm range,but without caudal MPD dilatation on magnetic resonance cholangiopancreatography(MRCP).CASE SUMMARY A 60-year-old female patient underwent computed tomography for a follow-up of uterine cancer post-excision,which revealed pancreatic *** revealed an unclear MPD of the pancreatic body at a 20-mm length without caudal MPD ***,course observation was *** 24 mo,MRCP revealed an increased caudal MPD caliber and a larger pancreatic *** performed ERCP and detected atypical cells suspected of adenocarcinoma by serial pancreatic juice aspiration cytology *** performed a distal pancreatectomy and obtained a histopathological diagnosis of high-grade *** parenchyma invasion was not observed,and curative resection was *** High-grade Pan-IN may cause MPD narrowing in a long range without caudal MPD dilatation.

关键词： Pancreatic cancer Pancreatic intraepithelial neoplasm High-grade pancreatic intraepithelial neoplasm Magnetic resonance cholangiopancreatography Carcinoma in situ Case report

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Cryptogenic cirrhosis in the region where obesity is not prevalent

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World Journal of Gastroenterology 2006年第13期12卷 2080-2085页

作者： Hideyuki kojima Shinya Sakurai Masahiko Matsumura Norie Umemoto Masahito Uemura Hiroyo Morimoto Yasuhiro Tamagawa hiroshi Fukui Third Department of Internal Medicine Nara Medical University 840 Shijo-cho Kashihara-shiNara 634-8522Japan Department of General Medicine and Clinical Investigation Nara Medical University 840 Shijo-cho Kashihara-shiNara 634-8522Japan Department of Internal Medicine Kokuho Central Hospital 404-1 Miyako TawaramotochoNara 636-0302Japan

AIM： Recent studies have demonstrated that obesity is the common feature of cryptogenic cirrhosis （CC） and non-alcoholic steatohepatitis. However, there is little information on CC in the region where obesity is not prevalent, METHODS： The clinical features, and the liver-related morbidity and mortality of CC were analyzed in Japan where the prevalence of obesity is low. Among 652 cirrhotic patients, we identified 29 patients （4.4%） with CC. Of these, 24 CC patients who were followed up for more than 6 months were compared in a case-control study with age-, sex-, and Child-Pugh score-matched controls having cirrhosis of viral etiology. RESULTS： Obesity （BMI≥25 kg/m^2）, diabetes mellitus, and hypertriglyceridemia were more frequent, and the visceral fat area was larger in the CC patients than in the controls. The indices of insulin resistance were higher and the serum aminotransferase levels were lower in the CC patients than in the controls. Logistic regression analysis identified the elevated hemoglobin A1c, BMI ≥ 25 kg/m^2, and normal aminotransferase levels as independent predictors of CC. Kaplan-Meier analysis demonstrated lower occurrence of hepatocellular carcinoma and higher survival rate in the CC than in the controls in contrast to the similar cumulatlve probability of liverrelated morbidity between those ***： CC more frequently presents with the clinical features suggestive of non-alcoholic steatohepatitis compared with controls even in the region where obesity is not prevalent. The lower occurrence of hepatocellular carcinoma and higher survival rate may indicate an indolent clinical course in CC as compared with viral cirrhosis.

关键词： Ke Cryptogenic cirrhosis Viral cirrhosis Nonalcoholic steatohepatitis Hepatocellular carcinoma Case-control study

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Lithium Ion Transport Environment by Molecular Vibrations in Ion-Conducting Glasses

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Energy & Environmental Materials 2024年第3期7卷 133-142页

作者： Hiroki Yamada Koji Ohara Satoshi Hiroi Atsushi Sakuda Kazutaka lkeda Takahiro Ohkubo Kengo Nakada Hirofumi Tsukasaki hiroshi Nakajimai Laszlo Temleitner Laszlo Pusztai Shunsuke Ariga Aoto Matsuo Jiong Ding Takumi Nakano Takuya Kimura Ryo Kobayashi Takeshi Usuki Shuta Tahara Koji Amezawa Yoshitaka Tateyama Shigeo Mori Akitoshi Hayashi Diffraction and Scattering Division Japan Synchrotron Radiation Research Institute(JASRI)1-1-1 KoutoSayo-choSayo-gun Hyogo 679-5198Japan Faculty of Materials for Energy Shimane University1060Nishi-Kawatsu-ChoMatsue Shimane 690-8504Japan Department of Applied Chemistry Graduate School of EngineeringOsaka Metropolitan University1-1 Gakuen-choSakaiOsaka 599-8531Japan Institute of Materials Structure Science High Energy Accelerator Research Organization(KEK)203-1 ShirakataTokai-muraNaka-gunlbaraki 319-1106Japan Graduate School of Engineering Chiba University 1-33 Yayoi-cho Inage-kuChiba 263-8522Japan Department of Materials Science Graduate School of EngineeringOsaka Metropolitan University1-1 Gakuen-choSakaiOsaka 599-8531Japan Wigner Research Centre for Physics Konkoly Thegeút 29-33 H-1121Budapest Hungary International Research Organisation of Advanced Science and Technology(IROAST) Kumamoto University2-39-1 KurokamiChuo-kuKumamoto 860-8555Japan Department of Applied Physics Nagoya Institute of TechnologyGokisoShowaNagoya 466-8555Japan Faculty of Science Yamagata University1-4-12 KojirakawaYamagata-shiYamagata 990-5860Japan Faculty of Science University of the Ryukyus1 SenbaruNishihara-choOkinawa 903-0213Japan Institute of Multidisciplinary Research for Advanced Materials Tohoku University2-1-1 KatahiraAoba-kuSendai 980-8577Japan Center for Green Research on Energy and Environmental Materials(GREEN)and International Center for Materials Nanoarchitectonics(MANA) National Institute for Materials Science(NIMS)TsukubaIbaraki 305-0044Japan

Controlling Li ion transport in glasses at atomic and molecular levels is key to realizing all-solid-state batteries,a promising technology for electric *** this context,Li_(3)PS_(4)glass,a promising solid electrolyte candidate,exhibits dynamic coupling between the Li^(+)cation mobility and the PS_(4)^(3-)anion libration,which is commonly referred to as the paddlewheel *** addition,it exhibits a concerted cation diffusion effect(i.e.,a cation-cation interaction),which is regarded as the essence of high Li ion ***,the correlation between the Li^(+)ions within the glass structure can only be vaguely determined,due to the limited experimental information that can be ***,this study reports that the Li ions present in glasses can be classified by evaluating their valence oscillations via Bader analysis to topologically analyze the chemical *** is found that three types of Li ions are present in Li_(3)PS_(4)glass,and that the more mobile Li ions(i.e.,the Li3-type ions)exhibit a characteristic correlation at relatively long distances of ***,reverse Monte Carlo simulations combined with deep learning potentials that reproduce X-ray,neutron,and electron diffraction pair distribution functions showed an increase in the number of Li3-type ions for partially crystallized glass structures with improved Li ion transport *** results show order within the disorder of the Li ion distribution in the glass by a topological analysis of their ***,considering the molecular vibrations in the glass during the evaluation of the Li ion valences is expected to lead to the development of new solid electrolytes.

关键词： electrolytes ionic conductors modeling molecular dynamics

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Identification of individuals with non-alcoholic fatty liver disease by the diagnostic criteria for the metabolic syndrome

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World Journal of Gastroenterology 2012年第13期18卷 1508-1516页

作者： Masahide Hamaguchi Noriyuki Takeda Takao kojima Akihiro Ohbora Takahiro Kato hiroshi Sarui Michiaki Fukui Chisato Nagata Jun Takeda Immunology Frontier Research Center at Osaka University Osaka 5650871Japan Department of Endocrinology and Metabo lism Murakami Memorial HospitalAsahi UniversityGifu 5008523Japan Department of Gastroenterology Murakami Memorial HospitalAsahi UniversityGifu 5008523Japan Department of Endocrinology and Metabolism Graduate School of Medical ScienceKyoto Prefectural University of MedicineKyoto 6028566Japan Department of Epidemiology and Preventive Medicine Graduate School of MedicineGifu UniversityGifu 5008523Japan Department of Diabetes and Endocrinology Division of Molecule and StructureGraduate School of MedicineGifu UniversityGifu 5008523Japan

AIM:To clarify the efficiency of the criterion of metabolic syndrome to detecting non-alcoholic fatty liver disease(NAFLD).METHODS:Authors performed a cross-sectional study involving participants of a medical health checkup program including abdominal *** study involved 11 714 apparently healthy Japanese men and women,18 to 83 years of *** was defined by abdominal ultrasonography without an alcohol intake of more than 20 g/d,known liver disease,or current use of *** revised criteria of the National Cholesterol Education Program Adult Treatment PanelⅢ were used to characterize the metabolic ***:NAFLD was detected in 32.2%(95%CI:31.0%-33.5%)of men(n=1874 of 5811)and in 8.7%(95%CI:8.0%-9.5%)of women(n=514 of 5903).Among obese people,the prevalence of NAFLD was as high as 67.3%(95%CI:64.8%-69.7%)in men and 45.8%(95%CI:41.7%-50.0%)in *** NAFLD was thought of as being the liver phenotype of metabolic syndrome,the prevalence of the metabolic syndrome among subjects with NAFLD was low both in men and women.66.8%of men and 70.4%of women with NAFLD were not diagnosed with the metabolic syndrome.48.2%of men with NAFLD and 49.8%of women with NAFLD weren't overweight[body mass index(BMI)≥25 kg/m2].In the same way,68.6%of men with NAFLD and 37.9%of women with NAFLD weren't satisfied with abdominal classification(≥90 cm for men and≥80 cm for women).Next,authors defined it as positive at screening for NAFLD when participants satisfied at least one criterion of metabolic *** sensitivity of the definition"at least 1 criterion"was as good as 84.8%in men and 86.6%in *** subjects by BMI,the sensitivity was higher in obese men and women than in non-obese men and women(92.3%vs 76.8%in men,96.1%vs 77.0%in women,respectively).CONCLUSION:Authors could determine NAFLD effectively in epidemiological study by modifying the usage of the criteria for metabolic syndrome.

关键词： Nonalcoholic fatty liver Metabolic syndrome Population based study Methodology

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A Case Report of Kissing Molars Class II

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Open Journal of Stomatology 2024年第5期14卷 249-254页

作者： Toshiyuki Inou Keiichi Uchida Keiko Kaneko Anna Fuji Nina Wakimoto Tatsuo Takaya Hiroyuki Kitamura hiroshi Mori Yuji Kurihara Department of Oral Diagnostics and Comprehensive Dentistry Matsumoto Dental University Hospital Shiojiri Japan Department of Oral and Maxillofacial Surgery School of Dentistry Matsumoto Dental University Shiojiri Japan

Kissing molars (KMs) is a condition of occlusal molar surfaces in a single follicular space with roots extending in opposite directions. Here, we have reported a case of KMs in a 58-year-old woman. The patient complained of pain in the right mandibular molar region and was diagnosed with KMs consisting of the right mandibular second and third molars. Because of the patient’s pain having subsided at the time of the visit and her unwillingness to undergo tooth extraction, the patient was followed up. KMs is classified into three classes (I-III) and is either true-KMs or pseudo-KMs and presents with or without cystic variants of dental follicles. The presents as true-KMs class II without a cystic variant. With reference to the literature and based on our analysis, the mean age of patients affected by this specific case of KMs is 31.7 years and unilateral KMs is relatively more common (85.7%). Histopathological findings of dentigerous cysts are more often indicated (42.9%). The treatment policy for KMs should therefore be based on the classification of KMs. Importantly, the focus should be on preserving the first and second molars as much as possible. The treatment approach, such as the employment of surgical removal or orthodontics, should be determined by considering the associated factors such as the crowns, tissues, and age of the patient.

关键词： Kissing Molars Mandibular Second Molar Mandibular Third Molar Cone-Beam Computed Tomography

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Accuracy and Utility of Vessel Analysis Using Non-Contrast CT for Planning Endovascular Aortic Repair

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Open Journal of Medical Imaging 2024年第3期14卷 96-105页

作者： Midori Komita-Moriya Yukihisa Ogawa Akiyuki Kotoku Shintaro Nawata Kenji Kuramochi Yasuyoshi Ogawa Kiyoshi Chiba hiroshi Nishimaki Hidefumi Mimura Department of Radiology St. Marianna University School of Medicine Kanagawa Japan Department of Radiology Tokai University School of Medicine Hachioji Hospital Tokyo Japan Radiological Technology St. Marianna University School of Medicine Kanagawa Japan Department of Cardiovascular Surgery St. Marianna University School of Medicine Kanagawa Japan

Objectives: This study aimed to determine whether errors in vascular measurements would affect device selection in endovascular aortic repair (EVAR) by comparing measurements obtained using non-contrast computed tomography (NCT) with those obtained using contrast-enhanced CT (CECT). Materials and Methods: This single-center, retrospective study included 25 patients who underwent EVAR for abdominal aortic aneurysm at our institution. A 1-mm horizontal cross-sectional slice of NCT and CECT from each patient was retrospectively reviewed. The area from the abdominal aorta to the common iliac artery was divided into four zones. A centerline was created using the NCT by manually plotting the center points. Subsequently, the centerlines were automatically extracted and manually corrected during the arterial phase of CECT. The diameter and length of each zone were measured for each modality. The mean diameters and lengths of the target vessels were compared between NCT and CECT. Results: The measurements obtained using both methods were reproducible and demonstrated good agreement. The mean differences in vessel length and diameter measurements for each segment between NCT and CECT were not statistically significant, indicating good consistency. Conclusion: NCT may be useful for preoperative EVAR evaluation in patients with renal dysfunction or allergies to contrast agents.

关键词： Non-Contrast CT Vessel Analysis EVAR Contrast-Enhanced CT Abdominal Aortic Aneurysm

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