Lysosomal Enzyme Activities in Cultured Retinal Pigment Epithelial and Glial Cells of RCS Rat

作     者：Hiroaki Naka Hiroshi Kuriyama Shinsuke Kojima Shunji Tsuboi 

作者机构：Itami Research LaboratorySenju pharmaceutical Co.Ltd.1-1SakuragaokaOshikaItami 664JapanItami Research LaboratorySenju pharmaceutical Co.Ltd.1-1SakuragaokaOshikaItami 664JapanInstitute of OphthalmologyTane Memorial Eye Hospital1-1-39 SakaigawaNishi-KuOsaka 550JapanInstitute of OphthalmologyTane Memorial Eye Hospital1-1-39 SakaigawaNishi-KuOsaka 550Japan 

出 版 物：《Eye Science》 (眼科学报（英文版）)

年 卷 期：1996年第16卷第1期

页      面：20-27页

学科分类：1002[医学-临床医学] 100212[医学-眼科学] 10[医学] 

主　　题：RCS rat retinal pigment epithelium glial cell lysosomal enzyme cell culture 

摘      要：Purpose: To compare the activities of acid phosphatase, N-acetyl-β-glu-cosaminidase and a- mannosidase in cultured retinal pigment epithelium (RPE)and glial cells of Royal College of Surgeons (RCS) rat with those in Long Evans(LE).Methods: The cultured RPE and glial cells of RCS and LE rat were plated into thesame 96 well microtitre, and the biochemical method in microsystem were usedfor enzyme ***: The activities of acid phosphatase and N-acetyl- β-glucosaminidase arehigher by, respectively, 30% and 46% in cultured RPE of RCS rat than LE *** activity of a- mannosidase has no significant difference. The activities of 3enzymes in the retinal glial cells derived from RCS rats are higher than LE rat by43% to 77%.Conclusion: These results suggest that the high activities of lysosomal enzymes inRCS RPE and glial cells may play an important role in the pathogenesis of retinaldystrophy. Eye Science 1996; 12:20-27.