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PiggyBac transposon-mediated gene delivery efficiently generates stable transfectants derived from cultured primary human deciduous tooth dental pulp cells(HDDPCs) and HDDPC-derived iPS cells

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International Journal of Oral Science 2015年第3期7卷 144-154页

作者： Emi Inada Issei Saitoh Satoshi Watanabe Reiji Aoki hiromi miura Masato Ohtsuka Tomoya Murakami Tadashi Sawami Youichi Yamasaki Masahiro Sato Department of Pediatric Dentistry Kagoshima University Graduate School of Medical and Dental Sciences Division of Pediatric Dentistry Graduate School of Medical and Dental Science Niigata University Animal Genome Research Unit Division of Animal Science National Institute of Agrobiological Sciences Functional Biomolecules Research Group National Agriculture and Food Research Organization Division of Basic Molecular Science and Molecular Medicine School of Medicine Tokai University Section of Gene Expression Regulation Frontier Science Research Center Kagoshima University

The ability of human deciduous tooth dental pulp cells（HDDPCs） to differentiate into odontoblasts that generate mineralized tissue holds immense potential for therapeutic use in the field of tooth regenerative medicine. Realization of this potential depends on efficient and optimized protocols for the genetic manipulation of HDDPCs. In this study, we demonstrate the use of a Piggy Bac（PB）-based gene transfer system as a method for introducing nonviral transposon DNA into HDDPCs and HDDPC-derived inducible pluripotent stem cells. The transfection efficiency of the PB-based system was significantly greater than previously reported for electroporation-based transfection of plasmid DNA. Using the neomycin resistance gene as a selection marker, HDDPCs were stably transfected at a rate nearly 40-fold higher than that achieved using conventional methods. Using this system, it was also possible to introduce two constructs simultaneously into a single cell. The resulting stable transfectants, expressing td Tomato and enhanced green fluorescent protein, exhibited both red and green fluorescence. The established cell line did not lose the acquired phenotype over three months of culture. Based on our results, we concluded that PB is superior to currently available methods for introducing plasmid DNA into HDDPCs. There may be significant challenges in the direct clinical application of this method for human dental tissue engineering due to safety risks and ethical concerns. However, the high level of transfection achieved with PB may have significant advantages in basic scientific research for dental tissue engineering applications, such as functional studies of genes and proteins. Furthermore, it is a useful tool for the isolation of genetically engineered HDDPC-derived stem cells for studies in tooth regenerative medicine.

关键词： drug selection electroporation genetically modified human deciduous tooth dental pulp cells Piggy Bac

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Education Programs for Invasive Procedures Involving Nurses: A Scoping Review

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Open Journal of Nursing 2024年第5期14卷 200-224页

作者： hiromi Shibuya Akiko Saito Masumi Mugiyama Noyuri Yamaji Chisato Eto Satoshi Shibuya Department of Nursing Faculty of Health Science Technology Bunkyo Gakuin University Tokyo Japan Department of Health Care Policy and Health Economics Institute of Medicine University of Tsukuba Ibaraki Japan Institute of Clinical Epidemiology Showa University Tokyo Japan Department of Family Nursing Division of Health Science and Nursing Graduate School of Medicine The University of Tokyo Tokyo Japan Department of Global Health Nursing Graduate School of Nursing St. Luke&#8217s International University Tokyo Japan School of Nursing Tokyo Women&#8217s Medical University Tokyo Japan Department of Integrative Physiology School of Medicine Kyorin University Tokyo Japan

Background: Implementing invasive procedures is an important part of patient management by clinical nurses;however, there are gaps between nurses’ actual knowledge and skills and expected professional care capacity. Purpose: This scoping review aimed to map the existing literature related to recent institution-provided educational programs for invasive procedures involving clinical nurses. This study seeks to understand the contents of educational programs and the methods for assessing educational effects. Methods: This scoping review was completed using the following four databases: PubMed (MEDLINE), Embase, Cochrane Library, and Emcare for the period 2000-2022. We included studies that used all forms of educational approaches (e.g., didactic lectures, hands-on training, or on-the-job training). This scoping review considered peer-reviewed publications published in English using quantitative, qualitative, or mixed approaches. A total of 83 studies underwent in the final analysis. Results and Conclusion: A combination of didactic lectures and hands-on training was provided as an educational program in most studies. Contrary to our prediction, educational interventions with advanced technologies such as VR are extremely rare, suggesting that the effectiveness of advanced technologies in learning invasive procedures should be examined to facilitate and retain educational effects more efficiently in future studies. Regarding the assessment of educational effects, nurses’ cognitive (i.e., theoretical knowledge about procedures), psychomotor (i.e., implementing procedures), and psychological aspects (e.g., confidence and self-efficacy in procedures) were evaluated using questionnaires and observational methods. While most studies used a one-group pretest-posttest design, the ratio of randomized controlled trials (RCT) was relatively low. Thus, an RCT design should be introduced in future studies to test the validity of the developed educational program more accurately.

关键词： Training Education Invasive Procedure Nurses Assessment

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热压缩同时提高挤压镁-锰-锌合金的强度和热导率(英文)

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Transactions of Nonferrous Metals Society of China 2024年

作者：刘海峰杨续跃张宇修 hiromi Nagaumi 赵明纯石智勇 Andrej Atrens School of Mechanical & Mining Engineering University of Queensland Brisbane 中南大学材料科学与工程学院苏州大学钢铁学院

在这项研究中，对挤压镁-锰-锌合金进行了热压缩处理。合金在 150°C以 15:1 的挤压比挤压，然后沿着挤压方向以真应变为0.9进行热压缩。通过光学显微镜、扫描电子显微镜、电子背散射衍射和透射电子显微镜研究了挤压态和热压缩态Mg-Mn-Z... 详细信息

在这项研究中，对挤压镁-锰-锌合金进行了热压缩处理。合金在 150°C以 15:1 的挤压比挤压，然后沿着挤压方向以真应变为0.9进行热压缩。通过光学显微镜、扫描电子显微镜、电子背散射衍射和透射电子显微镜研究了挤压态和热压缩态Mg-Mn-Zn合金的微观结构、机械性能和热导率。旨在通过获得均匀、细小的显微结构、以及降低织构强度，同时提高强度和导热性。屈服强度（YS）、极限拉伸强度（UTS）和伸长率（EL）得到了显著的提高，分别提高了77%、53%和10%。热导率也得到了显著的提高，从111 W/(m·K)提高到了125 W/(m·K)。本研究阐明了热压缩改善性能的机理，为开发具有优良热性能和力学性能的镁合金提供了一条有前景的途径。

关键词：镁合金热压缩强度热导率

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利用探针跳跃式扫描离子电导显微镜技术揭示结直肠癌单细胞层面对H2O2良性应激的动态响应（英文）

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Science Bulletin 2024年第12期 1909-1919页

作者：王东 Emily Woodcock 杨羲 hiromi Nishikawa Elena V.Sviderskaya Masanobu Oshima Christopher Edwards 张彦军 Yuri Korchev WPI Nano Life Science Institute (WPI-Nano LSI) Kanazawa University Department of Medicine Imperial College London Cell Biology Research Centre Molecular and Clinical Sciences Research Institute St George's University of London

Colorectal cancer（CRC）, a widespread malignancy, is closely associated with tumor microenvironmental hydrogen peroxide（H2O2） levels. Some clinical trials targeting H2O2for cancer treatment have revealed its paradoxical role as a promoter of cancer progression. Investigating the dynamics of cancer cell H2O2eustress at the single–cell level is crucial. In this study, non–contact hopping probe mode scanning ion conductance microscopy（HPICM） with high-sensitive Pt–functionalized nanoelectrodes was employed to measure dynamic extracellular to intracellular H2O2gradients in individual colorectal cancer Caco–2cells. We explored the relationship between cellular mechanical properties and H2O2gradients.Exposure to 0.1 or 1 mmol/L H2O2eustress increased the extracellular to intracellular H2O2gradient from 0.3 to 1.91 or 3.04, respectively. Notably, cellular F–actin–dependent stiffness increased at 0.1 mmol/L but decreased at 1 mmol/L H2O2eustress. This H2O2–induced stiffness modulated AKT activation positively and glutathione peroxidase 2（GPX2） expression negatively. Our findings unveil the failure of some H2O2-targeted therapies due to their ineffectiveness in generating H2O2, which instead acts eustress to promote cancer cell survival. This research also reveals the complex interplay between physical properties and biochemical signaling in cancer cells' antioxidant defense, illuminating the exploitation of H2O2eustress for survival at the single–cell level. Inhibiting GPX and/or catalase（CAT） enhances the cytotoxic activity of H2O2eustress against CRC cells, which holds significant promise for developing innovative therapies targeting cancer and other H2O2-related inflammatory diseases.

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Artificial oxygen carriers,from nanometer-to micrometer-sized particles,made of hemoglobin composites substituting for red blood cells

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Particuology 2022年第5期20卷 43-55页

作者： Takashi Matsuhira hiromi Sakai Department of Chemistry Nara Medical University840 Shijo-choKashihara 634-8521Japan

Blood transfusions are regarded as the most well-known and frequently performed cell transplantations.Although current transfusion systems are sophisticated,they cannot be freed from the inherent difficulties that include infection,short shelf life,and blood type mismatching.Artificial oxygen carriers produced using hemoglobin(Hb)are designated as Hb-based oxygen carriers(HBOCs),which are anticipated for use as biomaterials that have potential to resolve issues of transfusion by a radical paradigm shift.Various HBOCs,nanometer-sized to micrometer-sized bioparticles having an oxygen-carrying function,are developed for use as substitutes for red blood cells(RBCs).This paper presents an overview of the classification of HBOCs with reference to their histories,preparations,structures,functions,and in vitro and in vivo properties.Additionally,we give a more detailed introduction of our academic studies of liposome encapsulated Hb,designated as Hb-vesicles(HbV),which mimic the physiologically important corpuscular structure of RBCs.This review outlines perennial efforts and approaches to mimic RBC functions through chemical,genetic,and encapsulation techniques.It will provide important insights into the eventual realization of an alternative for RBC transfusion.

关键词： Blood substitutes Cross-linking Hemoglobin(Hb)-based oxygen carriers(HBOCs) Liposome Supramolecular assembly Transfusion alternatives

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新能源汽车用铝合金激光-电弧复合焊接研究进展(特邀)

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中国激光 2024年第4期51卷 23-40页

作者：王晓南陈夏明环鹏程李响董其鹏骆顺存长海博文苏州大学沙钢钢铁学院江苏苏州215021 江苏省新能源汽车用金属结构材料绿色制备与资源再生工程研究中心江苏苏州215021 东北大学轧制技术及连轧自动化国家重点实验室辽宁沈阳110819 无锡锐科光纤激光技术有限责任公司江苏无锡214000

发展新能源汽车是我国从汽车大国迈向汽车强国的必由之路,是应对气候变化、推动绿色发展的战略举措。近年来,作为新能源汽车制造的重要结构材料,车用铝合金性能的不断提升给后续的焊接加工带来了全新的挑战。与传统弧焊热源相比,激光-... 详细信息

发展新能源汽车是我国从汽车大国迈向汽车强国的必由之路,是应对气候变化、推动绿色发展的战略举措。近年来,作为新能源汽车制造的重要结构材料,车用铝合金性能的不断提升给后续的焊接加工带来了全新的挑战。与传统弧焊热源相比,激光-电弧复合热源具有诸多优势,为抑制铝合金焊接缺陷并提升焊接接头系数提供了新途径。较为全面地总结了近年来国内外学者在铝合金焊接缺陷(如软化、气孔)等形成及调控方面的研究进展,分析了现有研究工作中存在的问题并给出了激光-电弧复合焊接铝合金未来的研究方向,旨在为后续研究及应用提供参考。

关键词：激光技术激光-电弧复合焊接铝合金软化气孔力学性能

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Grain size and texture changes of magnesium alloy AZ31 during multi-directional forging

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中国有色金属学会会刊：英文版 2008年第A1期18卷 200-204页

作者：杨续跃孙争艳 XING 3Jie miura hiromi SAKAI Taku School of Materials Science and Engineering Central South University Changsha 410083 China Key Laboratory of Nonferrous Metal Materials Science and Engineering Ministry of Education Central South University Changsha 410083 China Department of Mechanical Engineering and Intelligent Systems UEC Tokyo Tokyo 182-8585 Japan

Grain size and texture changes of magnesium alloy AZ31 were studied in multidirectional forging(MDF) under decreasing temperature conditions.MDF was carried out up to large cumulative strains of 4.8 with changing the loading direction during decrease in temperature from pass to pass.MDF can accelerate the uniform development of fine-grained structures and increase the plastic workability at low temperatures.As a result,the MDFed alloy shows excellent higher strength as well as moderate ductility at room temperature even at the grain size below 1μm.Superplastic flow takes place at 423 K and depends on the anisotropy of MDFed samples.The mechanisms of strain-induced fine-grained structure development and of the plastic deformation were discussed in detail.

关键词：镁合金 AZ31镁合金常温金属功能材料

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Ru complex and N,P-containing polymers confined within mesoporous hollow carbon spheres for hydrogenation of CO_(2)to formate

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Nano Research 2023年第4期16卷 4515-4523页

作者： Guoxiang Yang Yasutaka Kuwahara Kohsuke Mori Catherine Louis hiromi Yamashita Division of Materials and Manufacturing Science Graduate School of EngineeringOsaka University2-1 Yamada-okaSuitaOsaka 565-0871Japan Institute for Open and Transdisciplinary Research Initiatives(OTRI) Osaka University2-1 Yamada-okaSuitaOsaka 565-0871Japan Unit of Elements Strategy Initiative for Catalysts&Batteries(ESICB) Kyoto UniversityKatsuraKyoto 615-8520Japan JST PRESTO4-1-8 HonchoKawaguchiSaitama 332-0012Japan Sorbonne Université CNRSLaboratoire de Réactivitéde SurfaceLRSF-75005 ParisFrance

The development of reliable catalysts with both excellent activity and recyclability for carbon dioxide(CO_(2))hydrogenation is challenging.Herein,a ternary hybrid heterogeneous catalyst,involving mononuclear Ru complex,N,P-containing porous organic polymers(POPs),and mesoporous hollow carbon spheres(Ru^(3+)-POPs@MHCS)is reported for CO_(2)hydrogenation to formate.Based on comprehensive structural analyses,we demonstrated that Ru^(3+)-POPs were successfully immobilized within MHCS.The optimized Ru^(3+)-0.5POPs@MHCS catalyst,which was obtained with about 5 wt.%Ru^(3+)and 0.5 mmol POPs polymers confined into 0.3 g MHCS,exhibited high catalytic activity for CO_(2)hydrogenation to formate(turnover number(TON)>1,200 for 24 h under mild reaction conditions(4.0 MPa,120℃))and improved durability,compared to Ru^(3+)catalysts without POPs polymers(Ru^(3+)-MHCS)and unencapsulated MHCS(Ru^(3+)-0.5POPs)catalysts.The improved catalytic performance is attributed to the high surface area and large pore volume of MHCS which favors dispersion and stabilization of Ru^(3+)-POPs.Furthermore,the MHCS and POPs showed high CO_(2)adsorption ability.Ru^(3+)-POPs encapsulated into MHCS reduces the activation energy barrier for CO_(2)hydrogenation to formate.

关键词： heterogeneous catalysis porous organic polymers(POPs) mesoporous hollow carbon spheres CO_(2)hydrogenation formate synthesis

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Liposome-Encapsulated Hemoglobin Vesicle Improves Persistent Anti-arrhythmogenesis through Improving Myocardial Electrical Remodeling and Modulating Cardiac Autonomic Activity in a Hemorrhagic Shock-Induced Rat Heart Model

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Current Medical Science 2023年第2期43卷 232-245页

作者： Bonpei Takase Yuko Higashimura Haruka Asahina Masayuki Ishihara hiromi Sakai Department of Intensive Care Medicine National Defense Medical CollegeTokorozawa359-8513Japan Division of Traumatology Research Institute National Defense Medical College Research InstituteTokorozawa359-8513Japan Division of Biomedical Engineering National Defense Medical College Research InstituteTokorozawa359-8513Japan Department of Chemistry School of MedicineNara Medical UniversityNara634-8521Japan

Objective Shock heart syndrome(SHS)is associated with lethal arrhythmias(ventricular tachycardia/ventricular fibrillation,VT/VF).We investigated whether liposome-encapsulated human hemoglobin vesicles(HbVs)has comparable persistent efficacy to washed red blood cells(wRBCs)for improving arrhythmogenesis in the subacute to chronic phase of SHS.Methods Optical mapping analysis(OMP),electrophysiological study(EPS),and pathological examinations were performed on blood samples from Sprague-Dawley rats following induction of hemorrhagic shock.After hemorrhagic shock,the rats were immediately resuscitated by transfusing 5%albumin(ALB),HbV,or wRBCs.All rats survived for 1 week.OMP and EPS were performed on Langendorff-perfused hearts.Spontaneous arrhythmias and heart rate variability(HRV)were evaluated using awake 24-h telemetry,cardiac function by echocardiography,and pathological examination of Connexin43.Results OMP showed significantly impaired action potential duration dispersion(APDd)in the left ventricle(LV)in the ALB group whereas APDd was substantially preserved in the HbV and wRBCs groups.Sustained VT/VF was easily provoked by EPS in the ALB group.No VT/VF was induced in the HbV and wRBCs groups.HRV,spontaneous arrhythmias,and cardiac function were preserved in the HbV and wRBCs groups.Pathology showed myocardial cell damage and Connexin43 degradation in the ALB group,all of which were attenuated in the HbV and wRBCs groups.Conclusion LV remodeling after hemorrhagic shock caused VT/VF in the presence of impaired APDd.Similar to wRBCs,HbV persistently prevented VT/VF by inhibiting persistent electrical remodeling,preserving myocardial structures,and ameliorating arrhythmogenic modifying factors in the subacute to chronic phase of hemorrhagic shock-induced SHS.

关键词： artificial oxygen carrier hemorrhage lethal arrhythmia resuscitation heart rate variability

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Numerical simulation of macrosegregation in billet continuous casting influenced by electromagnetic stirring

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Journal of Iron and Steel Research(International) 2022年第4期29卷 612-627页

作者： Qi-peng Dong Jiong-ming Zhang Yan-bin Yin hiromi Nagaumi School of Iron and Steel High-Performance Metal Structural Materials Research InstituteSoochow UniversitySuzhou 215021JiangsuChina State Key Laboratory of Advanced Metallurgy University of Science and Technology BeijingBeijing 100083China

A three-dimensional numerical model coupling the macrosegregation and magnetohydrodynamic simulations was developed to investigate the effects of electromagnetic stirring(EMS)on the macrosegregation.The results show that a significant swirling flow was induced by the in-mold EMS,which further changed the shape of the solidification shell and homogenized the solute elements in mold.However,the effects were only confined to the initial billet shell.The improvement in centerline segregation was observed with the usage of the final EMS(F-EMS),which led to the forced convection at the final solidification stage.The solute elements in the mushy zone were significantly even,with the maximum segregation degree of solute C reducing from 1.311 to 1.237.In addition,the effects of the stirrer positions and currents of F-EMS on the macrosegregation alleviation were numerically studied.Different values of centerline segregation were predicted with various stirrer positions and currents,and there is an optimum stirrer parameter to obtain the best macrosegregation alleviation.In the experimental conditions,the optimum position was about 7 m away from meniscus,and the optimum current was 300 A.

关键词： Numerical simulation Macrosegregation Electromagnetic stirring Continuous casting

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