检索结果-南通市图书馆

Matrix attachment regions included in a bicistronic vector enhances and stabilizes follistatin gene expressions in both transgenic cells and transgenic mice

在线全文

学校读者我要写书评

暂无评论

Frontiers of Agricultural Science and Engineering 2016年第1期3卷 87-96页

作者： Xiaoming HU Jing GUO Chunling BAI Zhuying WEI Li GAO Tingmao HU Shorgan bou Guangpeng LI Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology/Key Laboratory of Herbivore Reproductive Biotechnology and Breeding of Ministry of Agriculture Inner Mongolia University

In the present study, follistatin(FST) gene expression vectors with either a bicistronic gene transfer cassette alone, or a bicistron gene cassette carrying a matrix attachment region(MAR) were constructed and transfected to bovine fetal fibroblasts. Evaluations of both the integration and expression of exogenous FST indicated that the p MAR-CAG-FST-IRES-Ac GFP1-poly A-MAR(pMAR-FST) vector had higher capacity to form monoclonal transgenic cells than the vector without MAR,though transient transfection and integration efficiency were similar with either construct. Remarkably, protein expression in transgenic cells with the p MAR-FST vector was significantly higher than that from the bicistronic vector. Exogenous FST was expressed in all of the p MARFST transgenic mice at F_0, F_1 and F_2. Total muscle growth in F_0 mice was significantly greater than in wild-type mice,with larger muscles in fore and hind limbs of transgenic mice. p MAR-FST transgenic mice were also found with more evenly distributed muscle bundles and thinner spaces between sarcolemma, which suggests a correlation between transgene expression-associated muscle development and the trend of muscle growth. In conclusion, a p MAR-FST vector, which excluded the resistant genes and frame structure, enhances and stabilizes FST gene expressions in both transfected cells and transgenic mice.

关键词： safety of transgenic bicistron gene transfer body transgenic mice muscle development

Invitation to Contribute to FASE

在线全文

学校读者我要写书评

暂无评论

Frontiers of Agricultural Science and Engineering 2014年第2期 180-181页

作者： Shorgan bou Weilun Yin Ning Li the Chinese Academy of Engineering(CAE) Chinese Society of Forestry Chinese Association of Agricultural Science Societies the Poplar Committee of China State Key Laboratory for Agrobiotechnology in China Agricultural University Beijing Association for Science and Technology

Colleagues,It is our great pleasure to introduce you to the Frontiers of Agricultural Science and Engineering(FASE),a new international journal launched in *** was founded jointly by the Chinese Academy of Engineering...

关键词：

Invitation to Contribute to FASE

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Frontiers of Agricultural Science and Engineering 2014年第4期 350-351页

作者： Shorgan bou Weilun Yin Ning Li the Chinese Academy of Engineering(CAE) Chinese Society of Forestry Chinese Association of Agricultural Science Societies the Popiar Committee of China State Key Laboratory for Agrobiotechnology in China Agricultural University Beijing Association for Science and Technology

关键词：

新型冠状病毒疫情期间黎巴嫩护士的心理韧性及影响因素

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

International Journal of Nursing Sciences 2021年第4期8卷 432-438,I0005页

作者： Mohamad Alameddine Karen bou-Karroum Wahida Ghalayini Firas Abiad University of Sharjah College of Health SciencesSharjahUnited Arab Emirates Faculty of Health Sciences Department of Health Management and PolicyAmerican University of BeirutBeirutLebanon Rafik Hariri University Hospital BeirutLebanon Department of General Surgery American University of Beirut Medical CenterBeirutLebanon

目的本研究旨在探讨新型冠状病毒疫情期间黎巴嫩主要转诊中心护士的心理韧性水平及其影响因素。方法研究采用横断面调查设计。2020年春季,通过网络调查265名护士,调查内容包括5个方面:人口统计学资料、工作满意度、离职倾向、工作场所... 详细信息

目的本研究旨在探讨新型冠状病毒疫情期间黎巴嫩主要转诊中心护士的心理韧性水平及其影响因素。方法研究采用横断面调查设计。2020年春季,通过网络调查265名护士,调查内容包括5个方面:人口统计学资料、工作满意度、离职倾向、工作场所暴力和心理韧性现状。采用多元线性回归分析确定心理韧性的影响因素。结果结果显示,护士心理韧性总分为(66.91±13.34)分;67.8%护士对自己的工作满意,76.2%护士表示不太可能在未来一年内辞去目前的工作。在过去一年中有74.7%护士表示遭受过工作场所暴力。多元线性回归显示,对工作满意、男性、没有离职倾向及未遭受过职场暴力的护士心理韧性水平更高(P<0.05)。结论提高护士的心理韧性水平,可以提高他们在危机时期的工作满意度及留任率,并有助于提高护理服务的时效性。护理管理者应定期调查护士的心理韧性水平,并采取干预措施促进其提高,特别是在危机时期。

关键词：新型冠状病毒肺炎黎巴嫩护士韧性,心理

Association of problematic internet use with depression,impulsivity,anger,aggression,and social anxiety:Results of a national study among Lebanese adolescents

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Pediatric Investigation 2021年第4期5卷 255-264页

作者： Chadia Haddad Diana Malaeb Hala Sacre Jad bou Khalil Wael Khansa Roula Al Hajj Nelly Kheir Sylvia Saade Sahar Obeid Souheil Hallit Research Department Psychiatric Hospital of the CrossJal EddibLebanon INSERM Univ.LimogesCHU EsquirolIRDU1094 Tropical NeuroepidemiologyInstitute of Epidemiology and Tropical NeurologyGEISTLimogesFrance School of Pharmacy Lebanese International UniversityBeirutLebanon Life sciences and health department Paris-Est UniversityParisFrance INSPECT-LB:Institut National de SantéPublique Epidémiologie Clinique et ToxicologieBeirutLebanon Faculty of Medicine and Medical Sciences Holy Spirit University of Kaslik(USEK)JouniehLebanon Faculty of Arts and Sciences Holy Spirit University of Kaslik(USEK)JouniehLebanon

Importance:Several studies conducted worldwide(mostly in Western countries)highlighted the negative effects of problematic internet use,particularly among adolescents aged 12 to 17,including depression,impulsivity,aggression,and social fear and *** Lebanon,literature on the prevalence and impact of problematic internet use among adolescents is ***:This study aim was to study the association between problematic internet use and depression,impulsivity,anger,aggression and social phobia among Lebanese ***:A cross-sectional study was performed on 1103 young adolescents(14-17 years),recruited from October 2017 till April *** Internet Addiction Test(IAT)was used to evaluate the level of problematic internet *** were analyzed using the MANCOVA *** main independent variable of interest was the IAT,while the dependent variables included the psychological ***:The multivariate analysis taking the psychological scales as the dependent variables and the problematic internet use(IAT score)as an independent variable,showed that problematic internet use was associated with higher depression,impulsivity,aggression,anger,hostility and social ***:Problematic internet use has become an important health issue that should not be overlooked,particularly because of the increased use of the internet by *** programs on early exposure to the internet should be developed.

关键词： Problematic internet use Adolescents Depression Aggression Social phobia Hostility

Attenuation mechanism of Brucella melitensis M5-10,implications for vaccine development and differential diagnosis

在线全文

学校读者我要写书评

暂无评论

Frontiers of Agricultural Science and Engineering 2014年第4期1卷 331-340页

作者： Yuanqiang ZHENG Yanchun SHI Chang AN Ruisheng LI Dongjun LIU Yuehua KE Kairong MAO Mingjuan YANG Zeliang CHEN Shorgan bou National Research Center for Animal Transgenic Biotechnology Inner Mongolia UniversityHohhot 010021China Inner Mongolia Key Laboratory of Molecular Biology Inner Mongolia Medical UniversityHohhot 010058China Animal Laboratory Center 302 Hospital of PLABeijing 100039China Institute of Disease Control and Prevention Academy of Military Medical SciencesBeijing 100071China China Institute of Veterinary Drug Control Center for Veterinary Drug EvaluationMinistry of AgricultureBeijing 100081China College of Medicine Shihezi UniversityShihezi 832003China

Brucellosis is a worldwide *** is the most efficient means to prevent and control *** current licensed attenuated vaccines for animal use were developed by sequential passage in nonnatural hosts that decreased virulence in its original *** attenuation mechanism of these strains remains largely *** the present study,we sequenced the genome of Brucella melitensis vaccine strain *** analysis showed that a large number of genetic changes occurred in the vaccine strains.A total of 2854 genetic polymorphic sites,including 2548 SNP,241 INDEL and 65 MNV were *** the 2074 SNPs in coding regions,1310(63.2%)were non-synonymous *** number,percent and N/S ratios were disproportionally distributed among the cog *** polymorphic sites were identified in genes of the virB operon,flagella synthesis,and virulence regulating *** data indicate that changes in some cog categories and virulence genes might result in the *** attenuation mechanisms also have implications for screening and development of new vaccine *** genetic changes in the genome represent candidate sites for differential diagnosis between these vaccine strains and other virulence *** analysis of virulence genes showed that expression of dnaK,vjbR were reduced in M5-10 strain when compared with that in 16M.A duplex PCR targeting virB6 and dnaK was successfully used to differentiate between M5-10 and the virulent 16M *** genome re-sequencing technique represents a strong strategy not only for evaluation of vaccines,but also for development of new vaccines.

关键词： Brucella live attenuated vaccine attenuation differential diagnosis

Venous Thromboembolic Disease in a Regional Hospital in Mali

在线全文

学校读者我要写书评

暂无评论

World Journal of Cardiovascular Diseases 2023年第12期13卷 837-844页

作者： Coulibaly Souleymane Taoré Djénébou Sidibé Samba Sako Mariam Guindo Aissata Sanogo Alpha Kodio Aniéssa Dao Aissata B. A. Hamidou Oumar Diakité Mamadou Konaté Massama Menta Ichaka Cardiology Department CHU Point G Bamako Mali Department of Internal Medicine CHU Point G Bamako Mali Cardiology Department Nianankoro Fomba Hospital in Ségou Ségou Mali Cardiology Department CHU Gabriel Touré Bamako Mali Mali Hospital Medicine Service Bamako Mali

Introduction: Venous thromboembolism (VTE) is a nosological group which mainly includes deep vein thrombosis (DVT) and pulmonary embolism (PE), it is frequently associated with high morbidity and mortality. We initiated this study with the aim of studying VTE in a cardiological hospital environment in a regional hospital in Mali. Methodology: This was a descriptive study with prospective recruitment over 1 year from June 20, 2019 to June 20, 2020, covering patients hospitalized and followed in consultation in the cardiology department of the Ségou regional hospital in Mali. Results: We collected 31 cases of VTE out of 366 patients, representing a frequency of 8.47%. The sex ratio was 0.88. VTE risk factors were dominated by immobilization (29.03%), pregnancy and postpartum (16.12%), heart failure (16.12%). The reason for consultation was dyspnea (93.54%) followed by chest pain (83.87%). On admission the clinical manifestations were tachycardia (74.19%), tachypnea (90.32%), muffled heart sounds (70.96%), global heart failure syndrome (51.6%). According to VTE probability scores;51.61% of patients had an intermediate clinical probability according to the simplified Wells score for PE and 54.84% had an intermediate clinical probability for the simplified Geneva score for DVT. D-Dimers were only performed in 12 patients (38.70%) and were elevated in all. A chest CT angiogram showed PE in 90% of patients (n = 28/31). Venous Doppler ultrasound of the lower limbs showed venous thrombosis in 3 patients. Conclusion: Venous thromboembolism, although underdiagnosed, is common in our health structures. Prevention, particularly heparinoprophylaxis and early recovery in a hospital environment, remains the effective means of combating this condition.

关键词： Venous Thrombosis Pulmonary Embolism Nianankoro Fomba Hospital Ségou

The ecological adaptability of cloned sheep to free-grazing in the Tengger Desert of Inner Mongolia,China

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Frontiers of Agricultural Science and Engineering 2014年第3期1卷 191-200页

作者： Xinxin LI Huijuan WANG Guanghua SU Zhuying WEI Chunling BAI Wuni-MENGHE Yanhui HOU Changqing YU Shorgan bou Guangpeng LI The Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology Key Laboratory of Herbivore Reproductive Biotechnology and Breeding of Ministry of AgricultureInner Mongolia UniversityHohhot 010070China The Inner Mongolia Rangeland Ecology Institute Alashan 750306China

Since the birth of the first cloned sheep,somatic cell nuclear transfer technology has been successfully used to clone a variety of *** livestock have no apparent health risks,and the quality and safety of the cloned animal products are similar to non-cloned *** social behavior and environmental adaptability of postnatal cloned animals,especially when used for grassland farm production purposes,is *** the present study,the cloned Dorper sheep equipped with GPS location devices were free-grazed in a harsh natural environment similar to conditions commonly experienced by Mongolian *** main findings of this research were as follows.(1)Under free-grazing conditions,the cloned sheep showed excellent climatic and ecological *** extreme temperature conditions ranging from–30 to 40°C,the cloned sheep maintained acceptable body condition and behaved as other sheep.(2)The cloned sheep quickly adapted from a herd feeding strategy to the harsh environment and quickly exhibited a grazing regimen as other free-grazing sheep.(3)The cloned sheep exhibited free-grazing patterns and social behavior as other sheep.(4)The cloned sheep in the harsh environment thrived and produced healthy ***,the cloned Dorper sheep exhibited excellent ecological adaptation,which is an important consideration for breeding meat sheep by *** Dorper sheep readily adapted to the free-grazing conditions on the Mongolian plateau grassland,which attests to their ability to withstand harsh environmental conditions.

关键词： somatic cell nuclear transfer free-grazing synchronization Dorper sheep cloned animal ecology

In vivo and in vitro development of Tibetan antelope(Pantholops hodgsonii)interspecific cloned embryos

在线全文

学校读者我要写书评

暂无评论

Frontiers of Agricultural Science and Engineering 2014年第1期1卷 21-29页

作者： Guanghua SU Lei CHENG Yu GAO Kun LIU Zhuying WEI Chunling BAI Fengxia YIN Li GAO Guangpeng LI Shorgan bou The Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology Key Laboratory of Herbivore Reproductive Biotechnology and Breeding Ministry of AgricultureInner Mongolia UniversityHohhot 010021China

The Tibetan antelope is endemic to the Tibetan Plateau,China,and is now considered an endangered *** a possible rescue strategy,the development of embryos constructed by interspecies somatic cell nuclear transfer(iSCNT)was *** antelope fibroblast cells were transferred into enucleated bovine,ovine and caprine *** cloned embryos were then cultured in vitro or in the oviducts of intermediate *** than 0.5%of the reconstructed antelope-bovine embryos cultured in vitro developed to the blastocyst ***,when the cloned antelope-bovine embryos were transferred to caprine oviducts,about 1.6%of the embryos developed to the blastocyst *** contrast,only 0.7%of the antelope-ovine embryos developed to the morula stage and none developed to blastocysts in ovine *** treatment of donor cells and bovine oocytes with trichostatin A did not improve the embryo development even when cultured in the oviducts of ovine and *** the antelope-bovine embryos,constructed from oocytes treated with roscovitine or trichostatin A,were cultured in rabbit oviducts 2.3%and 14.3%developed to blastocysts,*** is concluded that although some success was achieved with the protocols used,interspecies cloning of Tibetan antelope presents difficulties still to be *** mechanisms resulting in the low embryo development need investigation and progress might require a deeper understanding of cellular reprogramming.

关键词： interspecific nuclear transfer bovine ovine caprine oviduct apoptosis