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In‑depth proteome characterization of endometrium and extraembryonic membranes during implantation in pig

Journal of Animal Science and Biotechnology 2024年第3期15卷 1027-1055页

作者： Maria A.Gil Josep M.Cambra heriberto rodriguez‑martinez Cristina Cuello Inmaculada Parrilla Emilio A.martinez Department of Medicine and Animal Surgery International Excellence Campus for Higher Education and Research“Campus Mare Nostrum”University of MurciaMurciaSpain Institute for Biomedical Research of Murcia(IMIB-Pascual Parrilla) MurciaSpain Technical University of Munich MunichGermany Department of Biomedical&Clinical Sciences(BKV) BKH/Obstetrics&GynaecologyLinkoping UniversityLinkopingSweden

Background Proteome characterization of the porcine endometrium and extraembryonic membranes is important to understand mother-embryo *** this study,the proteome of the endometrium and cho-rioallantoic membrane was characterized in pregnant sows(PS)during early gestation(d 18 and 24 of gestation)and in the endometrium of non-pregnant sows(NPS)during the same days using LC-MS/MS *** UniProtKB database and ClueGO were used to obtain functional Gene Ontology annotations and biological and functional networks,*** Our analysis yielded 3,254 and 3,457 proteins identified in the endometrium of PS and NPS,respectively;of these,1,753 being common while 1,501 and 1,704 were exclusive to PS and NPS,*** addition,we iden-tified 3,968 proteins in the extraembryonic membranes of *** analyses of function revealed some proteins had relevance for the immune system process and biological adhesion in endometrium while the embryonic chorion displayed abundance of proteins related to cell adhesion and cytoskeletal organization,suggesting they dominated the moment of endometrial remodeling,implantation and adhesion of the lining *** are available via Pro-teomeXchange with identifier *** This is the first in-depth proteomic characterization of the endometrium and extraembryonic mem-branes during weeks 3 to 4 of gestation;data that contribute to the molecular understanding of the dynamic environ-ment during this critical period,associated with the majority of pregnancy losses.

关键词： Endometrium Extraembryonic membranes Implantation Pig Proteome

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In-depth proteome characterization of endometrium and extraembryonic membranes during implantation in pig.

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畜牧与生物技术杂志(英文版) 2024年第3期 1027页

作者： Maria A.Gil Josep M.Cambra heriberto rodriguez-martinez Cristina Cuello Inmaculada Parrilla Emilio A.martinez

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Men becoming fathers by injection were more often age intracytoplasmic sperm born small for gestational

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Asian Journal of Andrology 2017年第1期19卷 103-106页

作者： Susanne Liffner Mats Hammar Marie Bladh Elizabeth Nedstrand heriberto rodriguez martinez Gunilla Sydsjo Obstetrics and Gynecology Department of Clinical and Experimental Medicine Faculty of Medicine and Health Sciences Linkoping University SE-581 85 LinkopingSweden

Being born with nonoptimal birth characteristics decreases the chance of becoming a father. Urogenital malformations as well as metabolic syndrome are more common in men born small for gestational age （SGA） and could be contributing factors to the reduced fertility rate seen in these men. It could imply that men becoming fathers by assisted reproductive technology （ART） more often are born with low birth weight （LBW）, preterm, and/or SGA than men conceiving without treatment and also that men where intracytoplasmic sperm injection （ICSI） had to be performed more often are born with nonoptimal birth characteristics than men where conventional in vitro fertilization （IVF） successfully could be used. In this retrospective, case-control study using Swedish national registers, we compared the birth characteristics of 1206 men who have become fathers by ART with a control group consisting of age-matched men who became fathers without treatment. The differences in birth characteristics between men becoming fathers by IVF and ICSI were also assessed. For men becoming fathers by ART, OR of being born with LBW was 1.66 （95% CI = 1.17-2.36） compared with fathers who conceived without treatment. OR of being born prematurely was 1.32 （95% CI = 1.00-1.77）. Men becoming fathers via ICSI had a doubled increased likelihood of being born SGA compared with men who became fathers via IVF （OR = 2.12; 95% CI = 1.17-3.83）. In conclusion, we have found that men becoming fathers by ICSI treatments had more often been born SGA than men becoming fathers by conventional IVF.

关键词： infertility intracytoplasmic sperm injection in vitro fertilization low birth weight preterm small for gestational age

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Colloid Centrifugation of Semen: Applications in Assisted Reproduction

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American Journal of Analytical Chemistry 2016年第8期7卷 597-610页

作者： Jane M. Morrell heriberto rodriguez-martinez Division of Reproduction Clinical Sciences Swedish University of Agricultural Sciences Uppsala Sweden Division of Clinical Sciences/O&G Department of Clinical and Experimental Medicine Linkoping University Linkoping Sweden

Colloid centrifugation can be used to separate heterogeneous populations of cells particularly semen samples, which typically contain mixtures of spermatozoa at different stage of maturity together with dead and dying spermatozoa and also non-sperm cells. Colloids can be used to enrich the population of spermatozoa that are viable and functional;these are the spermatozoa that are needed for fertilization in Assisted Reproduction. Sperm samples obtained after colloid centrifugation may be enriched for motile, morphologically normal spermatozoa with intact chromatin. Insemination of mares with stallion sperm samples prepared by colloid centrifugation resulted in a higher pregnancy rate per cycle than controls, confirming that the good sperm attributes observed in the laboratory were indicative of functional spermatozoa. The method does not simply enrich for viable spermatozoa by inhibiting dead or dying spermatozoa from passing through the colloid since emerging evidence shows that colloid centrifugation can select for spermatozoa with certain properties such as metabolic activity. Other reproductive cells, such as spermatids or progenitor cells, may also be purified by colloid centrifugation. This review describes the history of colloid centrifugation for selecting spermatozoa, compares colloid centrifugation with alternative techniques, and finally describes some of the applications of the technique within the animal breeding industry.

关键词： Animal Spermatozoa Purification Enrichment Selection Spermatids

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Flow cytometry for the assessment of animal sperm ntegrity and functionality： state of the art

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Asian Journal of Andrology 2011年第3期13卷 406-419,511页

作者： Md. Sharoare Hossain Anders Johannisson Margareta Wallgren Szabolcs Nagy Amanda Pimenta Siqueira heriberto rodriguez-martinez Department of Anatomy Physiology and Biochemistry Swedish University of Agricultural Sciences （SLU） SE-750 07 Uppsala Sweden Division of Reproduction SLU SE-750 07 Uppsala Sweden Quality Genetics HB SE-242 92 Horby Sweden University of Pannonia institute of Environmental Sciences H-B200 Veszprem Hungary Department of Clinical and Experimental Medicine Linkoping University SE-581 85 Linkoping Sweden

Flow cytometry is now a recognized methodology within animal spermatology, and has moved from being a research tool to become routine in the assessment of animal semen destined to breeding. The availability of ＇bench-top＇ flow cytometers and of newer and versatile markers for cell structure and function had allowed the instrumentation to measure more sperm parameters, from viability to reactiveness when exposed to exogenous stimuli, and to increase our capabilities to sort spermatozoa for potential fertilizing capacity, or chromosomal sex. The present review summarizes the state of the art regarding flow cytometry applied to animal andrology, albeit keeping an open comparative intent. It critically evaluates the present and future capabilities of flow cytometry for the diagnostics of potential fertility and for the development of current reproductive technologies such as sperm freezing, sperm selection and sperm sorting. The flow cytometry methods will probably further revolutionize our understanding of the sperm physiology and their functionality, and will undoubtedly extend its application in isolating many uncharacterized features of spermatozoa. However, continuous follow-up of the methods is a necessity owing to technical developments and the complexity of mapping spermatozoa.

关键词： animals fertility flow cytometry semen analysis spermatozoa sperm functionality sperm intactness

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Oxytocin in pig seminal plasma is positively related with in vivo fertility of inseminated sows

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Journal of Animal Science and Biotechnology 2022年第2期13卷 430-440页

作者： Lorena Padilla Marina López-Arjona Silvia martinez-Subiela heriberto rodriguez-martinez Jordi Roca Isabel Barranco Department of Medicine and Animal Surgery Faculty of Veterinary MedicineUniversity of MurciaE-30100 MurciaSpain Department of Biomedical&Clinical Sciences(BKV) BKH/Obstetrics&GynaecologyFaculty of Medicine and Health SciencesLinköping UniversitySE-58185 LinköpingSweden Department of Veterinary Medical Sciences University of BolognaIT-40064 Ozzano dell’EmiliaBolognaItaly

Background:Identification of relevant in vivo biomarkers for fertility remains a challenge for the livestock *** of the small peptide hormone oxytocin(OXT),involved in male reproductive function and present in the seminal plasma(SP)of several species could be a robust *** study characterized concentrations of SPOXT in ejaculates from boars used in artificial insemination(AI)programs aiming to evaluate its relationship with sperm quality variables and in vivo fertility of their liquid-stored *** OXT concentrations(ng/mL)were measured in 169 ejaculates from 61 boars of the Duroc,Pietrain,Landrace and Large White breeds using a direct competitive immunoassay test based on AlphaLISA®***(ejaculate volume,sperm concentration,total sperm count)and sperm parameters(motility,viability,intracellular generation of reactive oxygen species,plasma membrane fluidity)were assessed at 0 h and 72 h in AI-semen samples stored at 17℃.In vivo fertility included only 18 Large White and Landrace boars whose AI-semen was used to inseminated>100 sows and evaluated both farrowing rate and litter size of 3,167 ***:The results showed that SP-OXT differed between boars and between ejaculates within boar(P<0.05)but not between breeds(Duroc,Pietrain,Landrace and Large White).Ejaculates with higher SP-OXT concentration/mL(hierarchically grouped;P<0.001)had larger volume and came from younger boars(P<0.05).Ejaculates of boars showing positive farrowing rate deviation exhibited higher(P<0.05)SP-OXT concentration/mL than those with negative farrowing rate ***:The SP concentrations of OXT are boar,ejaculate and age dependent,and positively related with ejaculate volume and farrowing rates of liquid-stored semen AI-doses.

关键词： Artificial insemination Ejaculate Fertility Oxytocin Pig Semen quality Seminal plasma

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Energy model based on solar potential and the production of electrical energy through living plants’photosynthesis

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Clean Energy 2024年第3期8卷 116-126页

作者： Raymond Daniel rodriguez martinez Department of Industrial Production Faculty of EngineeringUniversidad Metropolitana1073 CaracasVenezuela

Developing a sustainable energy model is imperative considering the current trend towards decarbonizing sectors *** this purpose,Venezuela was used as a reference to propose an energy model focused on taking advantage of plant photosynthesis through microbial-vegetable fuel cells together with an agro-photovoltaic system to enhance energy and agricultural *** production from the cells was estimated using an average power density of 264 mW/m^(2) over 4%of the areas destined for crops in the entire Venezuelan region,obtaining an annual production of 19.889 GWh/*** contrast,the energy production of the agro-photovoltaic system was modelled using PVsyst software on 50%of the area used for the cells distributed throughout the states of Anzoátegui,Guárico,Monagas and Portuguesa according to their meteorological conditions,solar irradiation and agricultural activity,resulting in 3703417 GWh/*** resulting whole system proved to be able to cover>10 times the installed electricity generation capacity at a national level and,together with the tremendous scalability of the microbial fuel cells,it shows that Venezuela has a high potential for the production and distribution of clean energy.

关键词： ecosystems microbial fuel cell microorganisms renewable sustainability

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Plant-microbial fuel cell for electrical generation through living plants: an internal resistance insight into the plant species used

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Clean Energy 2024年第5期8卷 45-53页

作者： Raymond Daniel rodriguez martinez Department of Industrial Production Faculty of EngineeringUniversidad Metropolitana1073 CaracasVenezuela

Climate change is a global threat,the presence of which has encouraged the development and implementation of renewable energies,including plant–microbial fuel cells,which could generate 6629568–33147840 MWh per year due to their large-scale *** of the main challenges associated with microbial cells is internal resistance—a parameter whose magnitude is influenced by sev-eral *** the case of plant–microbial fuel cells,adding a plant positively affects the mitigation of internal ***,the species employed is expected to play an important *** the present study,the objective was to determine as a general reference the internal resistance of cells using various plant species,among which were tomato(Solanum lycopersicum),black beans(Phaseolus vulgaris),aloe vera(Aloe vera),corn(Zea mays),and moss(Dicranidae).For this purpose,an experimental procedure was carried out to compare the manual voltage measurements with a free cell concerning the voltage obtained by adding an external resistor in *** internal resistance values were determined as a function of the measured voltage,obtaining 177,179,175,324,and 233 kΩfor aloe vera,corn,tomato,black beans,and moss,*** this,it was shown that the roots of the plants represent an essen-tial addition to the internal resistance of the cell in the short *** contrast,plants with a C4 photosynthetic metabolism are more favourable,while C3 plants can also benefit from internal resistance during a more extended period of rhizodeposition.

关键词： climate change energy microorganism renewable source voltage

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Lesser kestrels of the same colony do not overwinter together

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Current Zoology 2024年第4期70卷 505-512页

作者： Jorge Garcia-Macia Munir Chaouni Sara Morollon Javier Bustamante Lina Lopez-Ricaurte Juan martinez-Dalmau Beatriz rodriguez-Moreno Vicente Urios Grupo de Investigacion Zoologia de Vertebrados Universidad de AlicanteApdo.99E-03080 AlicanteSpain Department of Wetland Ecology Estacion Biologica de Donana(CSIC)C/Americo Vespucio 2641092SevillaSpain GREFA C/MontedelPilarS/NE-28220MajadahondaMadridSpain

Migratory connectivity describes the linkage between breeding and nonbreeding sites,having major ecological implications in birds:1 season influence the success of an individual or a population in the following *** studies on migratory connectivity have used large-scale approaches,often considering regional populations,but fine-scale studies are also necessary to understand colony *** lesser kestrel Falco naumanni,an insectivorous migratory raptor which form colonies during the breeding period,was considered to have strong connectivity based on regional ***,no small-scale studies on migratory connectivity have been ***,we GPS(Global Positioning System)-tracked 40 adult lesser kestrels from 15 different Spanish breeding colonies,estimating the overlap index between home ranges and the distance between their *** was found that lesser kestrels from the same breeding colony placed their nonbreeding areas at 347±281 km(mean±standard deviation)away from each other(range=23-990),and their home ranges over-lapped by 38.4±23.6%.No differences between intra-colony and inter-colony metrics were found,which suggests that lesser kestrels from the same breeding cluster do not overwinter together,but they spread out and mixed independently of the colony belonging throughout the nonbreeding range of the ***,this study highlights the importance of performing connectivity studies using fine-scale approaches.

关键词： Falco naumanni GPS telemetry migratory connectivity nonbreeding raptor spatial ecology wintering.

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Histopathological impact of SARS-CoV-2 on the liver:Cellular damage and long-term complications

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World Journal of Gastroenterology 2024年第22期30卷 2866-2880页

作者： Alfonso rodriguez-Espada Moises Salgado-de la Mora Briana Mariette rodriguez-Paniagua Nathaly Limonde la Rosa Monica Itzel martinez-Gutierrez Santiago Pastrana-Brandes Nalu Navarro-Alvarez Department of Molecular Biology Universidad Panamericana School of MedicineCampus MéxicoMexico 03920Mexico Department of Internal Medicine Instituto Nacional de Ciencias Médicas y Nutrición Salvador ZubiránMexico 14080Mexico Department of Surgery University of Colorado Anschutz Medical CampusDenverCO 80045United States PECEM Facultad de MedicinaUniversidad Nacional Autónoma de MéxicoMexico 04360Mexico Department of Gastroenterology Instituto Nacional de Ciencias Médicas y Nutrición Salvador ZubiránMexico 14080Mexico

Coronavirus disease 2019(COVID-19),caused by the highly pathogenic severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),primarily impacts the respiratory tract and can lead to severe outcomes such as acute respiratory distress syndrome,multiple organ failure,and *** extensive studies on the pathogenicity of SARS-CoV-2,its impact on the hepatobiliary system remains *** liver injury is commonly indicated by reduced albumin and elevated bilirubin and transaminase levels,the exact source of this damage is not fully *** mechanisms for injury include direct cytotoxicity,collateral damage from inflammation,drug-induced liver injury,and ischemia/***,evidence often relies on blood tests with liver enzyme *** this comprehensive review,we focused solely on the different histopathological manifestations of liver injury in COVID-19 patients,drawing from liver biopsies,complete autopsies,and in vitro liver *** present evidence of the direct impact of SARS-CoV-2 on the liver,substantiated by in vitro observations of viral entry mechanisms and the actual presence of viral particles in liver samples resulting in a variety of cellular changes,including mitochondrial swelling,endoplasmic reticulum dilatation,and hepatocyte *** ly,we describe the diverse liver pathology observed during COVID-19 infection,encompassing necrosis,steatosis,cholestasis,and lobular *** also discuss the emergence of long-term complications,notably COVID-19-related secondary sclerosing *** the histopathological liver changes occurring during COVID-19 infection is pivotal for improving patient recovery and guiding decision-making.

关键词： Liver SARS-CoV-2 COVID-19 Angiotensin-converting enzyme 2 Histopathology Liver biopsies Liver autopsy In vitro

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