Flow cytometry for the assessment of animal sperm ntegrity and functionality： state of the art
用流式细胞仪评估动物精子的完整性和功能性：最先进的技术作者机构：Department of Anatomy Physiology and Biochemistry Swedish University of Agricultural Sciences （SLU） SE-750 07 Uppsala Sweden Division of Reproduction SLU SE-750 07 Uppsala Sweden Quality Genetics HB SE-242 92 Horby Sweden University of Pannonia institute of Environmental Sciences H-B200 Veszprem Hungary Department of Clinical and Experimental Medicine Linkoping University SE-581 85 Linkoping Sweden
出 版 物：《Asian Journal of Andrology》 (亚洲男性学杂志（英文版）)
年 卷 期：2011年第13卷第3期
页 面：406-419,511页
核心收录：
学科分类：10[医学]
主 题：animals fertility flow cytometry semen analysis spermatozoa sperm functionality sperm intactness
摘 要：Flow cytometry is now a recognized methodology within animal spermatology, and has moved from being a research tool to become routine in the assessment of animal semen destined to breeding. The availability of ＇bench-top＇ flow cytometers and of newer and versatile markers for cell structure and function had allowed the instrumentation to measure more sperm parameters, from viability to reactiveness when exposed to exogenous stimuli, and to increase our capabilities to sort spermatozoa for potential fertilizing capacity, or chromosomal sex. The present review summarizes the state of the art regarding flow cytometry applied to animal andrology, albeit keeping an open comparative intent. It critically evaluates the present and future capabilities of flow cytometry for the diagnostics of potential fertility and for the development of current reproductive technologies such as sperm freezing, sperm selection and sperm sorting. The flow cytometry methods will probably further revolutionize our understanding of the sperm physiology and their functionality, and will undoubtedly extend its application in isolating many uncharacterized features of spermatozoa. However, continuous follow-up of the methods is a necessity owing to technical developments and the complexity of mapping spermatozoa.
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