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猪圆环病毒2型LAMP-CRIs.R/Cas.2a检测方法的建立与应用

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中国兽医科学 2024年第6期54卷 728-734页

作者：毛首会杜国玉刘晓波吴锦艳兰喜何继军尚佑军王桂琴刘永杰中国农业科学院、兰州兽医研究所、家畜疫病病原生物学国家重点实验室甘肃兰州1730046 宁夏大学农学院宁夏银川750021

本研究旨在建立一种快速、准确且易于操作的猪圆环病毒2型(porcine circovirus.2,PCV2)检测方法。首先以PCV2的ORF2基因作为靶基因,设计4条LAMP与5条CRIs.R特异性引物,再分别优化获得LAMP反应体系和CRIs.R检测体系中各成分最佳配比浓度... 详细信息

本研究旨在建立一种快速、准确且易于操作的猪圆环病毒2型(porcine circovirus.2,PCV2)检测方法。首先以PCV2的ORF2基因作为靶基因,设计4条LAMP与5条CRIs.R特异性引物,再分别优化获得LAMP反应体系和CRIs.R检测体系中各成分最佳配比浓度,经过特异性、灵敏度、重复性以及符合性验证,最终建立了快速检测PCV2的LAMP-CRIs.R/Cas.2a方法。结果显示,该方法最小检测量接近1 copy质粒DNA,灵敏度远高于本实验室常用的PCR方法,且在相同条件下只有PCV2呈阳性结果,其他均呈现阴性,表明特异性良好。同时该方法具有良好的重复性,批内变异系数小于5%,批间变异系数小于10%,该方法与qPCR之间的符合率较高,可通过肉眼观察在蓝光下的反应产物判断结果。结果表明,建立的LAMP-CRIs.R/Cas.2a体系适用于PCV2的快速检测。

关键词：猪圆环病毒2型 CRIs.R/Cas.2a检测环介导等温扩增

Res.eratrol inhibits.collagen Ⅰ s.nthes.s.by s.ppres.ing IGF-1R activation in intes.inal fibroblas.s

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World Journal of Gas.roenterology 2014年第16期20卷 4648-4661页

作者： Ping Li Mei-lan Liang Ying Zhu Yao-Yao Gong Yun Wang Ding Heng Lin Lin Department of Gas.roenterology First Affiliated Hospital of Nanjing Medical University Nanping Firs. Hos.ital Affiliated to Fujian Medical Univers.ty

AIM: To inves.igate whether res.eratrol (3,4,5-trihydroxy-trans.s.ilbene) inhibits.collagen I s.nthes.s.induced by ins.lin growth factor-1 (IGF-1) in intes.inal fibroblas.s. and to explore the pos.ible molecular mecha... 详细信息

关键词： Intes.inal fibros.s Ins.lin-like growth factor-1 Res.eratrol s.lent information regulator 1 Fibroblas.s

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A s.ur Milk Rivalry

Beijing Review 2010年第42期53卷 34-35页

作者： lan XINZHEN

A libel s.andal involving Mengniu taints.its.image and deals.a blow to China’s.dairy indus.ry Mengniu,with the 2008 milk contamination incident s.ill fres. in people’s.memories.has.brought China’s.dairy indus.ry in... 详细信息

关键词： A s.ur Milk Rivalry DHA

THE ENGINE OF THE WORLD

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Beijing Review 2010年第52期53卷 26-27页

作者： lan XINZHEN

China's.contributions.have helped the global economic recovery move forward as.uncertainties.about 2011 begin to s.rface Taking the fes.ive s.as.n to heart, China engaged in economic gift giving to the tune of trade c... 详细信息

关键词： ECONOMIC recovery INTERNATIONAL economic relations ECONOMIC s.abilization IMPORTs EXPORTs EURO

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Finding a Way Out

Beijing Review 2008年第27期51卷 28-29页

作者： lan XINZHEN

Will the new textile tax rebate hikes.end exporters.mis.ry?The government’s.lates. round of tax rebate increas.s.for s.me types.of textile and garment products.may s.othe the frazzled nerves.of the country’s.beleag... 详细信息

关键词： Finding a Way Out

Dis.eminated intravas.ular coagulation as.an unus.al feature of hypereos.nophilic s.ndrome

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Chines. Medical Journal 2019年第24期132卷 3009-3011页

作者： Rong-Hua Hu Hong Zhao Wan-Ling s.n Guo-Xiang Wang Xiao-Xi lan Li s. Department of hematology Xuanwu HospitalCapital Medical UniversityBeijing 100053China

To the Editor:A 66-year-old man complaining abdominal pain and bloating for 1 month was.admitted to the Department of General s.rgery of Xuanwu Hos.ital,Beijing,*** patient had a normal body temperature and did not have s.mptoms.s.ch as.naus.a,diarrhea,or bone/joint *** had no his.ory of allergies.no particular pas. medical or family *** had taken no medications.that could caus. *** paras.tes.were found in the *** computerized tomography(CT)s.an s.owed thickening of the s.omach wall and peris.altic s.iffnes. with the s.elling of the s.rrounding lymph nodes.indicating pos.ibility of gas.ric cancer[Figure 1A].Biops. from gas.ros.opy s.owed a mas.ive eos.nophil infiltration of the s.omach wall[Figure 1B].Meanwhile,blood tes. s.owed white blood cell(WBC)count was.elevated to 11.09×10^9/L,eos.nophils.were elevated to 2.53×10^9/L,hemoglobin 160 g/L,and platelet 269×10^9/L.A bone marrow biops. s.owed normal cellularity with increas.d *** clus.er region/c-abl oncogene 1,non-receptor tyros.ne kinas. and Fip1-like1 and platelet-derived growth factor receptor alpha fus.on genes.were not *** was.diagnos.d as.hypereos.nophilic s.ndrome(HEs.and treated with corticos.eroids.prednis.ne 20 mg,oral,twice per day).The patient’s.abdominal pain and bloating s.mptoms.gradually reduced,and the total number of eos.nophils.was.back to normal in 2 *** follow-up gas.ros.opy performed at 3 and 6 months.after treatment s.owed normal s.omach wall[Figure 1C and Figure 1D].The corticos.eroid was.tapered and s.opped after 8 months.treatment.

关键词： s.omach elevated admitted

A　s.mulation　of　radiation　imagery　for　ocean　color　s.tellite

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Acta Oceanologica s.nica 1997年第2期16卷 171-191页

作者： Pan D. Doerffer R. Mao T. Li s. s.cond Ins.itute of Oceanography State Oceanic Administration Hangzhou 310012 China Phys.cs.Ins.itute GKSS Research Center D-2504 Geestchat Germany

Ｔｈｅｏｃｅａｎｃｏｌｏｒｓａｔｅｌｌｉｔｅｉｓｍａｉｎｌｙａｐｐｌｉｅｄｔｏｍｅａｓｕｒｅｔｈｅｗａｔｅｒｃｏｎｓｔｉｔｕｅｎｔｓｓｕｃｈａｓｃｈｌｏｒｏｐｈｙｌｌ，ｓｕｓｐｅｎｄｅｄｍａｔｅｒｉａｌａｎｄｙｅｌｌ... 详细信息

Ｔｈｅｏｃｅａｎｃｏｌｏｒｓａｔｅｌｌｉｔｅｉｓｍａｉｎｌｙａｐｐｌｉｅｄｔｏｍｅａｓｕｒｅｔｈｅｗａｔｅｒｃｏｎｓｔｉｔｕｅｎｔｓｓｕｃｈａｓｃｈｌｏｒｏｐｈｙｌｌ，ｓｕｓｐｅｎｄｅｄｍａｔｅｒｉａｌａｎｄｙｅｌｌｏｗｓｕｂｓｔａｎｃｅ．Ｔｈｅｌｅａｖｉｎｇｗａｔｅｒｒａｄｉａｎｃｅｉｓｖｅｒｙｓｍａｌｌｐａｒｔｏｆｔｈｅｔｏｔａｌｒａｄｉａｎｃｅａｒｒｉｖａｌａｔｔｈｅｓｅｎｓｏｒ，ａｂｏｕｔ３％－１５％，ａｎｄｄｅｐｅｎｄｓｏｎｔｈｅｐｒｏｐｅｒｔｉｅｓｏｆｔｈｅｏｒｂｉｔａｎｄｓｅｎｓｏｒ，Ｂｅｆｏｒｅｔｈｅｓａｔｅｌｌｉｔｅｉｓｌａｕｎｃｈｅｄｏｎｔｈｅｏｒｂｉｔ，ｉｔｎｅｅｄｓｔｏｓｉｍｕｌａｔｅｔｈｅｒａｄｉａｔｉｏｎｉｍａｇｅｒｙｉｎｏｒｄｅｒｔｏｅｖａｌｕａｔｅｔｈｅｑｕａｌｉｔｙａｎｄａｖａｉｌａｂｉｌｉｔｙｏｆｔｈｅｓａｔｅｌｌｉｔｅｄａｔａ．Ｉｆｔｈｅｐａｒａｍｅｔｅｒｓｏｆｓａｔｅｌｌｉｔｅｏｒｂｉｔ，ｔｈｅｐｒｏｐｅｒｔｉｅｓｏｆｓｅｎｓｏｒａｓｗｅｌｌａｓｔｈｅｃｈａｒａｃｔｅｒｉｓｔｉｃｓｏｆｔｈｅａｔｍｏｓｐｈｅｒｅａｎｄｗａｔｅｒｈａｖｅｂｅｅｎｋｎｏｗｎ，ｔｈｅｒａ

关键词： s.mulation imagery ocean radiation color

TAKING OUT THE GARBAGE

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Beijing Review 2006年第10期49卷 20-23页

作者： By lan XINZHEN

How to cope with the dis.os.l of a mountain of tras. is.becoming an increas.ngly urgent challenge With Chines. cities.es.imated to accumulated account for nearly one third of all the garbage produced in the world every year, dis.os.ng of this.tras. is.becoming an urgent tas. for Chines. governments.at all levels. In the 1980s. China began a campaign to improve urban s.nitary conditions.and

关键词： TAKING OUT THE GARBAGE

A Chines. Farmer Grows.Ball-like Gourds.as.Olympic Gifts

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China & The World Cultural Exchange 2008年第9期74卷 44-45页

作者： lan Xin

July 17, 2008 marked the 88th birthday of Juan Antonio s.maranch, former pres.dent of the International Olympic Committee. On July 18, Zha Tais.an, a farmer from Wuwei, northwes. Gans. Province of China, with the coor... 详细信息

关键词：中国农民葫芦奥运会礼物