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Differential transcriptional regulation of the NANOG gene in chicken primordial germ cells and embryonic stem cells

Journal of Animal Science and Biotechnology 2021年第3期12卷 877-890页

作者： hee jung choi So Dam Jin Deivendran Rengaraj Jin Hwa Kim Bertrand Pain Jae Yong Han Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences Seoul National UniversitySeoul 08826South Korea Univ Lyon UniversitéLyon 1INSERMINRAEStem Cell and Brain Research InstituteU1208USC136169500 BronFrance Institute for Biomedical Sciences Shinshu UniversityMinamiminowaNagano 399-4598Japan

Background:NANOG is a core transcription factor(TF)in embryonic stem cells(ESCs)and primordial germ cells(PGCs).Regulation of the NANOG gene by TFs,epigenetic factors,and autoregulatory factors is well characterized in ESCs,and transcriptional regulation of NANOG is well established in these *** NANOG plays a key role in germ cells,the molecular mechanism underlying its transcriptional regulation in PGCs has not been ***,we investigated the mechanism that regulates transcription of the chicken NANOG(cNANOG)gene in PGCs and ***:We first identified the transcription start site of cNANOG by 5′-rapid amplification of cDNA ends PCR ***,we measured the promoter activity of various 5′flanking regions of cNANOG in chicken PGCs and ESCs using the luciferase reporter *** expression required transcriptional regulatory elements,which were positively regulated by POU5F3(OCT4)and SOX2 and negatively regulated by TP53 in *** proximal region of the cNANOG promoter contains a positive transcriptional regulatory element(CCAAT/enhancer-binding protein(CEBP)-binding site)in ***,small interfering RNA-mediated knockdown demonstrated that POU5F3,SOX2,and CEBP played a role in cell type-specific transcription of ***:We show for the first time that different trans-regulatory elements control transcription of cNANOG in a cell type-specific *** finding might help to elucidate the mechanism that regulates cNANOG expression in PGCs and ESCs.

关键词： Chicken Embryonic stem cells NANOG gene Primordial germ cells Regulatory elements

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Extracts from Paeonia lactiflora Pallas, Rehmannia Glutinosa var. Purpurea Makino, Perilla Frutescens var. Acuta Kudo may increase the endometrial receptivity through expression of leukemia inhibitory factor and adhesion molecules

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Journal of Traditional Chinese Medicine 2019年第1期39卷 15-25页

作者： Eun-Yeong Kim Tae-Wook Chung hee-jung choi Yeon-Seop jung Syng-Ook Lee Jun-Yong choi Hyung Sik Kim Sooseong You Myeong Soo Lee Ki-Tae Ha School of Korean Medicine and Korean Medical Research Center for Healthy Aging Pusan National UniversityYangsan 50612Republic of Korea Graduate Training Program of Korean Medicine for Healthy-aging Pusan National UniversityYangsan 50612Republic of Korea Department of Food Science and Technology Keimyung UniversityDaegu 42601Republic of Korea National Clinical Research Center for Korean Medicine Korean Medicine Hospital of Pusan National UniversityYangsan 50612Republic of Korea Laboratory of Molecular Toxicology School of PharmacySungKyunKwan UniversitySuwon 16419Republic of Korea Korea Institute of Oriental Medicine Daejeon 34054Republic of Korea

OBJECTIVE: To find out the combination of the extracts from Paeonia lactiflora Pallas(PL), Rehmannia Glutinosa var. Purpurea Makino(RG), Perilla Frutescens var. Acuta Kudo(PF) to increase endometrial ***: Herbal medicines were extracted with boiling water and polysaccharides were *** examined the effect of PL, RG, and PF(PRP), a most effective herbal formula deduced from constitutive ingredient herbs of Antai Yin which is composed of PRP, on the leukemia inhibitory factor(LIF) expression and endometrial ***: The combination of the extracts from PRP induced the LIF expression in Ishikawa cells and increased the adhesion between Ishikawa and JAr cells. In addition, PRP-induced attachment of JAr cells onto Ishikawa cells and expression of adhesion molecules, ITGAV, ITGB5, CD44 s, and Lselectin, are significantly reduced by knock-down of LIF ***: Induced by the combination of the PRP extracts, the adhesion between trophoblast and endometrial cells are mediated by expression of LIF and adhesion molecules. Thus, we suggest the combination of the PRP extracts may be a novel therapy for enhancing embryo implantation rate.

关键词： Paeonia Rehmannia Perilla Embryo implantation Endometrium Leukemia inhibitory factor Cell adhesion molecules

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Opuntia humifusa aqueous extract alleviates ethanol-induced gastric ulcer in a mouse model

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Asian Pacific Journal of Tropical Biomedicine 2020年第9期10卷 403-410页

作者： Chi-Yeol Yoo Hyeong-U Son Alshammari Fanar hee-jung choi Md Badrul Alam Sang-Han Lee Department of Food Science and Biotechnology Kyungpook National UniversityDaegu 41566Korea Food and Bio-Industry Research Institute Kyungpook National UniversityDaegu 41566Korea

Objective:To investigate the effect of Opuntia humifusa aqueous extract on gastric ***:An ethanol-induced model was used to examine the protective effect of Opuntia humifusa against gastric *** gastric ulcer index was evaluated via clinical observation and image *** inflammatory indicators were determined by RTPCR and Western blotting ***:The gastric ulcer index was reduced to 8%in the group treated with Opuntia humifusa aqueous extract compared with that in the control ***-PCR analysis revealed that MUC5 AC expression was reduced to 39%in the control group compared with the non-treated group,whereas the omeprazole and Opuntia humifusa aqueous extract-treated groups increased the expression to 95%and 79%,***,the expressions of various cytokines including TNF-α,IL-1β,and IL-6 were increased in the control group,while decreasing in Opuntia humifusa aqueous extract-treated *** humifusa aqueous extract also suppressed the expressions of iN OS,COX-2,and its transcription factor NF-κB and increased mucus content considerably as compared to the control ***:These results suggest that Opuntia humifusa aqueous extract is suitable as an alternative remedy for gastric ulcer treatment.

关键词： Opuntia humifusa Gastric ulcer Gastric lesions Image analysis Mucin

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Endovascular treatment of traumatic renal artery pseudoaneurysm with a Stanford type A intramural haematoma:A case report

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World Journal of Clinical Cases 2022年第27期10卷 9760-9767页

作者： Yook Kim Jin Young Lee Jin Suk Lee Jin Bong Ye Se Heon Kim Young Hoon Sul Su Young Yoon jung hee choi Hanlim choi Department of Radiology Chungbuk National University HospitalCheongju-si 28644Chungcheongbuk-doSouth Korea Department of Trauma Surgery Chungbuk National University HospitalCheongju-si 28644Chungcheongbuk-doSouth Korea Department of Trauma Surgery College of MedicineChungbuk National UniversityCheongju-si 28644Chungcheongbuk-doSouth Korea Cardiovascular and Thoracic Surgery Chungbuk National University HospitalCheongju-si 28644Chungcheongbuk-doSouth Korea Anesthesiology and Pain Medicine Chungbuk National University HospitalCheongju-si 28644Chungcheongbuk-doSouth Korea Department of Surgery Chungbuk National University HospitalCheongju-si 28644Chungcheongbuk-doSouth Korea

BACKGROUND Aortic intramural hematoma(IMH) associated with aortic branch tear and intramurally located pseudoaneurysm after blunt trauma has not been ***,we report a case of progressive type A aortic IMH associated with a pseudoaneurysm arising from the injured proximal renal artery after blunt *** SUMMARY During logging operations,a 66-year-old man experienced blunt force trauma after being injured by a fallen *** arrived at our trauma center with a left flank pain *** tomography(CT) revealed a pseudoaneurysm arising from the proximal renal artery(localized within the aortic media) and Stanford type A IMH.A covered stent was deployed along the left main renal artery,bridging the pseudoaneurysm and covering the parent artery,successfully excluding the pseudoaneurysm as confirmed using ***,although the degree of the pseudoaneurysm decreased,follow-up CT revealed remnant pseudoaneurysm,likely caused by an ***,a covered stent was additionally installed through the previously deployed covered *** exclusion of the pseudoaneurysm was confirmed using final *** the 7-mo follow-up CT scan,the IMH and pseudoaneurysm completely disappeared with no evidence of stent-related *** Endovascular treatment such as stent-graft placement can be an effective and safe treatment for traumatic renal artery injury.

关键词： Aortic intramural hematoma Blunt trauma Intimomedial tear Renal arterial injury Stent-graft Case report

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Production of germline chimeric quails following spermatogonial cell transplantation in busulfan-treated testis

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Asian Journal of Andrology 2018年第4期20卷 414-416页

作者： Young Min Kim Jin Se Park Jong Won Yoon hee jung choi Kyung Je Park Tamao Ono Jae Yong Han Department of Agricultural Biotechnology Research Institute of Agriculture and Life Sciences College of Agriculture and Life Sciences Seoul National University Seou108826 Korea Division of Animal Science Faculty of Agriculture Shinshu University Minamiminowa Nagano 399-4598 Japan Institute for Biomedical Sciences Shinshu University Minamiminowa Nagano 399-4598 Japan

Dear Editor, The merits of using quaff as an avian experimental model include high egg production, low maintenance cost, small body size, and short generation period （approximately 6-8 weeks）. Indeed, these characteristics make quail an ideal species for many biological areas including transgenic research. Methods of primordial germ cell （PGC）-mediated germline chimera production are considered very reliable and have been used in avian transgenesis.

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Development of the Guideline for HIV Testing in South Korea with Low Prevalence of HIV-1 Infection Based on the Expert Consensus

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World Journal of AIDS 2011年第4期1卷 155-164页

作者： hee jung choi Kyoung Ae Kong jung Won Min Hun-Jae Lee Hae-Sook Park 不详

Purpose: This study was conducted to develop a guideline for HIV testing in South Korea where the HIV prevalence is very low. It is necessary to make the recommendations presented here based on the epidemiology of opportunistic infection and cancer in the community. Methods: The development of a guideline for HIV testing was conducted using literature reviews and the agreement of Korean experts for HIV patients using a two-round Delphi consensus technique. Each expert was asked to independently answer to each item related to HIV risk. Ten experts participated in the two-round survey. Results: The individual items were determined based on the following categories: high risk behavior, symptom and diseases, colo-rectal diseases, uro-genital diseases and gynecology, skin and oral diseases. The final guideline consisted of 4-scale agreement rating (strongly recommended, recommended, not routinely recommended, no recommendation for routine provision). Discussion: We have developed the guideline for HIV testing in a country where the HIV prevalence is low based on a systematic investigation and expert consensus.

关键词： HIV Testing Guideline Consensus Delphi Studies

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Comprehensive molecular characterization to predict immunotherapy response in advanced biliary tract cancer:a phase II trial of pembrolizumab

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Oncology Research 2025年第1期33卷 57-65页

作者： RYUL KIM JOO KYUNG PARK MINSUK KWON MINAE AN jung YONG HONG JOON OH PARK SUNG hee LIM SEUNG TAE KIM Division of Hematology-Oncology Department of MedicineSamsung Medical CenterSungkyunkwan University School of MedicineSeoulKorea Division of Gastroenterology Department of MedicineSamsung Medical CenterSungkyunkwan University School of MedicineSeoulKorea Department of Hematology-Oncology Ajou University School of MedicineSuwon-siGyeonggi-doKorea Samsung Advanced Institute of Health Science and Technology Sungkyunkwan University School of MedicineSeoulKorea

Background:Immune checkpoint inhibitors(ICIs)are effective in a subset of patients with metastatic solid ***,the patients who would benefit most from ICIs in biliary tract cancer(BTC)are still *** and methods:We molecularly characterized tissues and blood from 32 patients with metastatic BTC treated with the ICI pembrolizumab as second-line ***:All patients had microsatellite stable(MSS)type *** of the 32 patients achieved partial response(PR),with an objective response rate(ORR)of 9.4%(95%confidence interval[CI],2.0–25.2)and nine showed stable disease(SD),exhibiting a disease control rate(DCR)of 37.5%(95%CI,21.1–56.3).For the 31 patients who had access to PD-1 ligand 1(PD-L1)combined positive score(CPS)testing(cut-off value≥1%),the ORR was not different between those who had PD-L1-positive(PD-L1+;1/11,9.1%)and PDL1-(2/20,10.0%)tumors(p=1.000).The tumor mutational burden(TMB)of PD-L1+BTC was comparable to that of PD-L1-BTC(p=0.630).TMB and any exonic somatic mutations were also not predictive of pembrolizumab *** analysis of blood and tumor samples demonstrated a relatively high natural killer(NK)cell proportion in the peripheral blood before pembrolizumab treatment in patients who achieved tumor ***,the tumors of these patients presented high enrichment scores for NK cells,antitumor cytokines,and Th1 signatures,and a low enrichment score for cancer-associated ***:This study shows the molecular characteristics associated with the efficacy of pembrolizumab in BTC of the MSS type.

关键词： Pembrolizumab Whole-exome sequencing Whole-transcriptome sequencing Biliary tract cancer

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Consistency of intensity ratio between spectral lines with similar self-absorption characteristics during ungated laser induced breakdown spectroscopy measurements

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Plasma Science and Technology 2019年第3期21卷 77-83页

作者： jung-Hwan IN Youngmin MOON Jang-hee choi Sungho JEONG Optical Lens Research Center Korea Photonics Technology Institute School of Mechanical Engineering Gwangju Institute of Science and Technology

This work reports that the intensity ratio of spectral lines having similar self-absorption characteristics during laser induced breakdown spectroscopy(LIBS) analysis can become nearly constant over a wide range of irradiation conditions if the intensities are integrated over a sufficiently long time. It is shown that the plasma temperature and intensity ratio of these spectral lines have temporal similarity. The spectral lines with similar self-absorption properties may be selected to improve the accuracy and consistency of LIBS analysis results under an environment with fluctuating measurement conditions.

关键词： laser induced breakdown spectroscopy intensity ratio self-absorption characteristics CIGS solar cell

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Topology optimization of reactive material structures for penetrative projectiles

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Defence Technology（防务技术） 2022年第7期18卷 1205-1218页

作者： Shinyu Kim Saekyeol Kim Taekyun Kim Sangin choi Tae hee Lee jung Su Park Sang-Hyun jung Department of Automotive Engineering Hanyang University222 Wangsimni-roSeongdong-guSeoul04763Republic of Korea The 4th R&D Institute Agency for Defense Development35Yuseong P.O.BoxDaejeon34186Republic of Korea

Recently,reactive materials have been developed for penetrative projectiles to improve impact resistance and energy ***,the design of a reactive material structure,involving shape and size,is challenging because of difficulties such as high non-linearity of impact resistance,manufacturing limitations of reactive materials and high expenses of penetration *** this study,a design optimization methodology for the reactive material structure is developed based on the finite element analysis.A finite element model for penetration analysis is introduced to save the expenses of the *** resistance is assessed through the analysis,and result is calibrated by comparing with experimental *** on the model,topology optimization is introduced to determine shape of the *** design variables and constraints of the optimization are proposed considering the manufacturing limitations,and the optimal shape that can be manufactured by cold spraying is *** on the optimal shape,size optimization is introduced to determine the geometric dimensions of the *** a result,optimal design of the reactive material structure and steel case of the penetrative projectile,which maximizes the impact resistance,is *** the design process proposed in this study,reactive material structures can be designed considering not only mechanical performances but also manufacturing limitations,with reasonable time and cost.

关键词： Reactive material Penetrative projectile Topology optimization Manufacturing constraint Cold gas dynamic spray Additive manufacturing

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High-Throughput Pb Recycling for Perovskite Solar Cells Using Biomimetic Whitlockite

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Energy & Environmental Materials 2023年第3期6卷 170-176页

作者： jung Sug Hong hee jung Kim Chang Hwun Sohn Oh Yeong Gong Jin Hyuk choi Kang hee Cho Gill Sang Han Ki Tae Nam Hyun Suk jung Department of Materials Science and Engineering Seoul National UniversitySeoul 08826Korea School of Advanced Materials Science and Engineering Sungkyunkwan UniversitySuwon 16419Korea

Pb contamination in aquatic environments causes severe pollution;therefore,harmless absorbents are *** this study,we report a novel synthesis of whitlockite(WH,Ca_(18)Mg_(2)(HPO_(4))_(2)(PO_(4))_(12)),which is the second most abundant biomineral in human bone,and its application as a high-performing Pb^(2+)***(HAP)and WH are prepared via a simple precipitation *** Pb2+absorption performance and mechanism of the synthesized biominerals are investigated in aqueous solutions at neutral *** results demonstrate that WH exhibits an excellent Pb2+absorption capacity of 2339 mg g^(−1),which is 1.68 times higher than the recorded value for ***,the absorbed Pb^(2+) ions are recycled into high-purity PbI_(2).This is employed as a precursor for the fabrication of perovskite solar cells(PSCs),resulting in a conversion efficiency of 19.00%comparable to that of commercial PbI2 powder(99.99%purity).Our approach provides an efficient way to remove Pb^(2+)ions from water and reuse them in the recycling of PSCs.

关键词： Pb absorption Pb recycling perovskite solar cells water purification whitlockite

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