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眼镜蛇毒细胞毒素缓释微球制备及体外性质研究

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中国生化药物杂志 2008年第3期29卷 161-164页

作者：陈志奎林礼务林琦庄权权何以敉薛恩生福建医科大学附属协和医院超声科福建医科大学医药生物工程中心福建医科大学蛇毒研究所福建福州3500041

目的研究眼镜蛇毒细胞毒素（Cytotoxin，CTX）聚乳酸／羟基乙酸缓释微球的制备方法，考察其一般性质、体外释药特性及生物学活性。方法采用色谱方法纯化眼镜蛇CTX，MTT方法检测细胞毒活性，复乳-溶剂挥发法制备载药微球，考察微球表面... 详细信息

目的研究眼镜蛇毒细胞毒素（Cytotoxin，CTX）聚乳酸／羟基乙酸缓释微球的制备方法，考察其一般性质、体外释药特性及生物学活性。方法采用色谱方法纯化眼镜蛇CTX，MTT方法检测细胞毒活性，复乳-溶剂挥发法制备载药微球，考察微球表面形态、粒径、包封率、载药率、体外释药行为及释放眼镜蛇CTX细胞毒活性。结果纯化眼镜蛇CTX具有明显的细胞毒作用，对肝癌hepG2细胞12，24h的IC50分别为1．43，1．12μg／mL，对L02肝细胞12，24h的IC50分别为1．37，1．01μg／mL。微球表面光滑圆整，粒径2．1～7．8μm，包封率和栽药率分别为（74．10±9．92）％和（0．72±0．09）％，30d药物累积释放84．3％，释放眼镜蛇CTX保持较好的生物学活性。结论采用复乳-溶剂挥发法可制备具有较高包封率，良好缓释效果，保持完整生物学活性的眼镜蛇CTX缓释微球。

关键词：细胞毒素聚乳酸-羟基乙酸微球缓释

三维超声及彩色多普勒成像对肝脏良恶性肿瘤的鉴别诊断

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中华医学超声杂志（电子版） 2005年第6期2卷 363-365页

作者：孙彦林礼务薛恩生林学英高上达何以敉福建医科大学附属协和医院超声科福建超声医学研究所

目的探讨三维超声及彩色多普勒成像对肝脏良恶性肿瘤鉴别诊断的价值。方法采用Aloka SSD-5500超声仪及TomTec三维成像系统,对37例肝脏恶性肿瘤及36例良性肿瘤患者行三维超声成像,观察并比较两组肿瘤内部血流分布、有无穿支血流及其血流... 详细信息

目的探讨三维超声及彩色多普勒成像对肝脏良恶性肿瘤鉴别诊断的价值。方法采用Aloka SSD-5500超声仪及TomTec三维成像系统,对37例肝脏恶性肿瘤及36例良性肿瘤患者行三维超声成像,观察并比较两组肿瘤内部血流分布、有无穿支血流及其血流信号容积与肿瘤体积比(Ⅵ)。结果肝脏良恶性肿瘤的外周血流检出率分别为88.89%(32/36)、78.835(29/37),两组间比较差异无统计学意义(P=0.343)；良恶性肿瘤的中心血流显示率分别为33.33%(12/36)、94.59%(35/37),穿支血流的检出率分别为5.56%(2/36)、62.16%(23/37),Ⅵ值分别为(4.56±0.92)、(18.98±1.87),两组间比较差异有非常显著性(P＜0.01)。结论三维超声及彩色多普勒成像为肝脏良恶性肿瘤的形态、血流分布及三维血流参数方面提供了更多的诊断信息,对鉴别诊断有一定的价值。

关键词：超声检查三维成像鉴别诊断肝脏良恶性肿瘤彩色多普勒三维超声

Additive neurorestorative effects of exercise and docosahexaenoic acid intake in a mouse model of Parkinson’s disease

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Neural Regeneration Research 2025年第2期20卷 574-586页

作者： Olivier Kerdiles Méryl-Farelle Oye mintsa mi-mba Katherine Coulombe Cyntia Tremblay VincentÉmond Martine Saint-Pierre Clémence Rouxel Line Berthiaume Pierre Julien Francesca Cicchetti Frédéric Calon Facultéde pharmacie UniversitéLaval1050 Avenue de la MédecineQuebecQCCanada Axe Neuroscience Centre de recherche du CHU de Québec-UniversitéLaval(Pavillon CHUL)2705 Boulevard LaurierQuebecQCCanada Institute of Nutrition and Functional Foods QuebecQCCanada Optinutribrain International Associated Laboratory(NutriNeuro FranceINAFCanada)QuebecQCCanada Axe Endocrinologie et Néphrologie Centre de recherche du CHU de Québec-UniversitéLavalQuebecQCCanada Département de Médecine Facultéde MédecineUniversitéLavalQuebecQCCanada Département de Psychiatrie et Neurosciences Facultéde MédecineQuebecQCCanada

There is a need to develop interventions to slow or reverse the degeneration of dopamine neurons in Parkinson’s disease after *** that preclinical and clinical studies suggest benefits of dietary n-3 polyunsaturated fatty acids,such as docosahexaenoic acid,and exercise in Parkinson’s disease,we investigated whether both could synergistically interact to induce recovery of the dopaminergic ***,mice received a unilateral stereotactic injection of 6-hydroxydopamine into the striatum to establish an animal model of nigrostriatal *** weeks after lesion,animals were fed a docosahexaenoic acid-enriched or a control diet for the next 8 *** this period,the animals had access to a running wheel,which they could use or *** acid treatment,voluntary exercise,or the combination of both had no effect on(i)distance traveled in the open field test,(ii)the percentage of contraversive rotations in the apomorphine-induction test or(iii)the number of tyrosine-hydroxylase-positive cells in the substantia nigra pars ***,the docosahexaenoic acid diet increased the number of tyrosine-hydroxylase-positive terminals and induced a rise in dopamine concentrations in the lesioned *** to docosahexaenoic acid treatment or exercise alone,the combination of docosahexaenoic acid and exercise(i)improved forelimb balance in the stepping test,(ii)decreased the striatal DOPAC/dopamine ratio and(iii)led to increased dopamine transporter levels in the lesioned *** present results suggest that the combination of exercise and docosahexaenoic acid may act synergistically in the striatum of mice with a unilateral lesion of the dopaminergic system and provide support for clinical trials combining nutrition and physical exercise in the treatment of Parkinson’s disease.

关键词： 6-hydroxydopamine dopamine dopamine transporter exercise neurorestoration Parkinson’s disease polyunsaturated fatty acids omega-3

含DG的交直流不平衡配电系统暂态电压稳定性分析

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电力自动化设备 2025年第1期 33-41页

作者：徐艳春王霖张涛王凌云 mi Lu 三峡大学梯级水电站运行与控制湖北省重点实验室德克萨斯农工大学电气与计算机工程学院

分布式电源（DG）以及大量恒功率直流负荷接入交直流配电系统使得母线暂态电压表现出正负极耦合且多波动的特性，现有暂态电压稳定性评估方法准确率低且适用性较差。推导分析了双极交直流配电系统直流侧线路、负荷参数不平衡时影响系统... 详细信息

分布式电源（DG）以及大量恒功率直流负荷接入交直流配电系统使得母线暂态电压表现出正负极耦合且多波动的特性，现有暂态电压稳定性评估方法准确率低且适用性较差。推导分析了双极交直流配电系统直流侧线路、负荷参数不平衡时影响系统母线暂态电压稳定的因素。基于二元表判据思想，提出一种改进的自适应母线暂态电压稳定性评估指标。针对参数不平衡时含DG的双极交直流配电系统，引入稳定性保持因子，提出母线与系统的暂态电压稳定裕度计算方法。基于所提方法利用MATLAB/Simulink仿真平台在改进的IEEE 33节点与IEEE 69节点双极交直流配电系统中量化影响系统暂态电压稳定性的因素，并对比验证了所提指标的有效性和优越性。

关键词：暂态电压稳定性交直流系统不平衡配电系统直流故障分析

DNA tetrahedron-mediated immune-sandwich assay for rapid and sensitive detection of PSA through a microfluidic electrochemical detection system

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microsystems & Nanoengineering 2021年第2期7卷 221-230页

作者： Dezhi Feng Jing Su yi Xu Guifang he Chenguang Wang Xiao Wang Tingrui Pan Xianting Ding Xianqiang mi Key Laboratory of Functional Materials for Informatics Shanghai Institute of Microsystem and Information TechnologyChinese Academy of Sciences200050ShanghaiChina Shanghai Advanced Research Institute Chinese Academy of Sciences201210ShanghaiChina University of Chinese Academy of Sciences 100049BeijingChina State Key Laboratory of Oncogenes and Related Genes Institute for Personalized MedicineSchool of Biomedical EngineeringShanghai Jiao Tong University200030ShanghaiChina School of Life Sciences Shanghai University200444ShanghaiChina Shenzhen Institutes of Advanced Technology Chinese Academy of Science518055ShenzhenChina CAS Center for Excellence in Superconducting Electronics (CENSE)200050ShanghaiChina Key Laboratory of Systems health Science of Zhejiang Province Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesChinese Academy of Sciences310024HangzhouChina

Prostate-specific antigen(PSA)is the most widely used biomarker for the early diagnosis of prostate *** methods for PSA detection are burdened with some limitations and require ***,we developed a novel microfluidic–electrochemical(μFEC)detection system for PSA ***,we constructed an electrochemical biosensor based on screen-printed electrodes(SPEs)with modification of gold nanoflowers(Au NFs)and DNA tetrahedron structural probes(TSPs),which showed great detection ***,we fabricated microfluidic chips by DNA TSP-Au NF-modified SPEs and a PDMS layer with designed dense meandering ***,theμFEC detection system was achieved based on microfluidic chips integrated with the liquid automatic conveying unit and electrochemical detection ***μFEC system we developed acquired great detection performance for PSA detection in PBS *** PSA assays in spiked serum samples of theμFEC system,we obtained a linear dynamic range of 1–100 ng/mL with a limit of detection of 0.2 ng/mL and a total reaction timehe“gold-standard”chemiluminescence assays and theμFEC *** terms of operation procedure,cost,and reaction time,our method was superior to the current methods for PSA detection and shows great potential for practical clinical application in the future.

关键词： fluid diagnosis system

Small extracellular vesicles derived from cerebral endothelial cells with elevated microRNA 27a promote ischemic stroke recovery

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Neural Regeneration Research 2025年第1期20卷 224-233页

作者： yi Zhang Zhongwu Liu michael Chopp michael millman Yanfeng Li Pasquale Cepparulo Amy Kemper Chao Li Li Zhang Zheng Gang Zhang Department of Neurology Henry Ford HospitalDetroitMIUSA Department of Physics Oakland UniversityRochesterMIUSA Department of Pathology Henry Ford HospitalDetroitMIUSA Division of Pharmacology Department of NeuroscienceReproductive and Dentistry SciencesSchool of MedicineUniversity of Naples“Federico Ⅱ”NaplesItaly

Axonal remodeling is a critical aspect of ischemic brain repair processes and contributes to spontaneous functional *** previous in vitro study demonstrated that exosomes/small extracellular vesicles(sEVs)isolated from cerebral endothelial cells(CEC-sEVs)of ischemic brain promote axonal growth of embryonic cortical neurons and that microRNA 27a(miR-27a)is an elevated miRNA in ischemic *** the present study,we investigated whether normal CEC-sEVs engineered to enrich their levels of miR-27a(27a-sEVs)further enhance axonal growth and improve neurological outcomes after ischemic stroke when compared with treatment with non-engineered CEC-sEVs.27a-sEVs were isolated from the conditioned medium of healthy mouse CECs transfected with a lentiviral miR-27a expression *** EVs isolated from CECs transfected with a scramble vector(Scra-sEVs)were used as a *** male mice were subjected to permanent middle cerebral artery occlusion and then were randomly treated with 27a-sEVs or *** array of behavior assays was used to measure neurological *** with treatment of ischemic stroke with Scra-sEVs,treatment with 27a-sEVs significantly augmented axons and spines in the peri-infarct zone and in the corticospinal tract of the spinal grey matter of the denervated side,and significantly improved neurological *** vitro studies demonstrated that CEC-sEVs carrying reduced miR-27a abolished 27a-sEV-augmented axonal *** analysis revealed that 27a-sEVs systemically administered preferentially localized to the pre-synaptic active zone,while quantitative reverse transcription-polymerase chain reaction and Western Blot analysis showed elevated miR-27a,and reduced axonal inhibitory proteins Semaphorin 6A and Ras Homolog Family Member A in the peri-infarct *** of the Clathrin-dependent endocytosis pathway substantially reduced neuronal internalization of *** data provide evidence that 27a-sEVs have

关键词： axonal remodeling cerebral endothelial cells exosomes miR-27a mitochondria Semaphorin 6A small extracellular vesicles stroke

蓖麻毒素温敏型凝胶经皮瘤内注射治疗移植肝癌的实验研究

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中华超声影像学杂志 2008年第7期17卷 631-633页

作者：陈志奎林礼务杨发端林晓东薛恩生郑斌王艳何以敉高上达福建医科大学附属协和医院超声科福州350001 福建医科大学附属协和医院病理科福州350001 福建医科大学附属协和医院药学部福州350001

目的探讨蓖麻毒素温敏型凝胶经皮瘤内注射治疗实验性肝癌的有效性和可行性。方法建立裸鼠皮下移植肝癌模型32只，随机分成4组，分别经皮瘤内注射生理盐水、空白凝胶、游离蓖麻毒素和蓖麻毒素温敏型凝胶，每周超声测量肿瘤体积，第7、14... 详细信息

目的探讨蓖麻毒素温敏型凝胶经皮瘤内注射治疗实验性肝癌的有效性和可行性。方法建立裸鼠皮下移植肝癌模型32只，随机分成4组，分别经皮瘤内注射生理盐水、空白凝胶、游离蓖麻毒素和蓖麻毒素温敏型凝胶，每周超声测量肿瘤体积，第7、14d每组处死裸鼠1只行肿瘤组织病理检查，余裸鼠记录生存时间。结果游离蓖麻毒素组治疗后7、14、21d肿瘤体积均小于生理盐水对照组（P〈0．01），生命延长率为36．8％；蓖麻毒素温敏型凝胶组治疗后第5d肝癌组织明显坏死，第3周肿瘤消退，病理检查见肿瘤组织大片坏死，伴大量炎性细胞浸润，裸鼠在生存时间的最终观察点均未见死亡。结论蓖麻毒素温敏型凝胶瘤内注射可彻底消融灭活肝癌，安全高效且操作简便。

关键词：超声检查肝肿瘤实验性蓖麻毒素温敏型凝胶

Fatal risk in hysteroscopic surgery that should not be overlooked:Uterine artery pseudoaneurysm

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World Journal of Clinical Cases 2025年第8期13卷 53-56页

作者： mi-Si he Ke-Xiao Yu Chen Wang Department of Gynecologic Oncology Chongqing University Cancer Hospital and Chongqing Cancer Institute and Chongqing Cancer HospitalChongqing 400030China Department of Orthopedics Chongqing Hospital of Traditional Chinese MedicineChongqing 400021China

This article provides a detailed account of the diagnosis and treatment of a case involving a uterine artery pseudoaneurysm(UAP),as well as an analysis of UAP *** finding emphasizes that UAP should be considered in patients presenting with abnormal genital bleeding after hysteroscopy and offers valuable insights and lessons for gynecologists in hysteroscopic *** patient underwent timely relevant examinations to confirm the diagnosis,allowing for crucial time required for her *** this study,the primary cause of UAP formation in the patient was attributed to a prior hysteroscopic surgical procedure conducted at another medical facility,suggesting that the selection and imple-mentation of dilatation catheters are some of the predisposing factors for *** conclusion,this case study offers a comprehensive analysis of the etiology of UAP and effectively provides timely diagnosis and treatment,offering valuable in-sights for the clinical diagnosis and management of UAP.

关键词： Editorial Uterine artery pseudoaneurysm Case report Hysteroscopic surgery Uterine arteriovenous malformations

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多西他赛共聚物纳米胶束的制备及性能研究

生物医学工程与临床 2011年第2期15卷 99-102页

作者：黄静魏洪芬杨菁林礼务何以敉薛恩生陈志奎福建省超声医学研究所福建福州350001 福建医科大学附属协和医院药学部福建福州350001 福建医科大学附属协和医院超声科福建福州350001

目的制备一种包载多西他赛的聚乳酸羟基乙酸-聚乙二醇-聚乳酸羟基乙酸(PLGA-PEG-PLGA)三嵌段共聚物纳米胶束,并考察其相关性能。方法采用开环聚合法合成共聚物,直接溶解法制备载多西他赛共聚物纳米胶束,荧光光谱法测定临界胶束浓度,高... 详细信息

目的制备一种包载多西他赛的聚乳酸羟基乙酸-聚乙二醇-聚乳酸羟基乙酸(PLGA-PEG-PLGA)三嵌段共聚物纳米胶束,并考察其相关性能。方法采用开环聚合法合成共聚物,直接溶解法制备载多西他赛共聚物纳米胶束,荧光光谱法测定临界胶束浓度,高效液相色谱检测载药胶束的包封率与载药率,透析法测定载药胶束体外释放情况,扫描电镜观察纳米胶束的形态,激光粒径仪测量共聚物纳米胶束粒径及分布,改良寇氏法测定载药胶束的半数致死量。结果直接溶解法制备的共聚物纳米胶束的临界胶束浓度为4.5×10-3 g/L,多西他赛与共聚物投料比为1∶20制备的载多西他赛共聚物纳米胶束的包封率为98.20%,载药率达4.68%,在体外平稳释放时间约3 d。扫描电子显微镜观察载多西他赛共聚物纳米胶束呈类圆形,分散良好,平均粒径为30.8 nm,多元分散系数0.42。载多西他赛共聚物纳米胶束静脉注射小鼠的半数致死量为273.5 mg/kg。结论采用直接溶解法可制备一种载多西他赛的PLGA-PEG-PLGA三嵌段共聚物纳米胶束,包封率高,体外释药平稳,毒副作用小,具有潜在的临床应用价值。

关键词：多西他赛聚合物胶束缓释材料纳米粒子聚乳酸羟基乙酸聚乙二醇