DNA tetrahedron-mediated immune-sandwich assay for rapid and sensitive detection of PSA through a microfluidic electrochemical detection system
作者机构：Key Laboratory of Functional Materials for InformaticsShanghai Institute of Microsystem and Information TechnologyChinese Academy of Sciences200050ShanghaiChina Shanghai Advanced Research InstituteChinese Academy of Sciences201210ShanghaiChina University of Chinese Academy of Sciences100049BeijingChina State Key Laboratory of Oncogenes and Related GenesInstitute for Personalized MedicineSchool of Biomedical EngineeringShanghai Jiao Tong University200030ShanghaiChina School of Life SciencesShanghai University200444ShanghaiChina Shenzhen Institutes of Advanced TechnologyChinese Academy of Science518055ShenzhenChina CAS Center for Excellence in Superconducting Electronics(CENSE)200050ShanghaiChina Key Laboratory of Systems Health Science of Zhejiang ProvinceHangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesChinese Academy of Sciences310024HangzhouChina
出 版 物：《Microsystems & Nanoengineering》 (微系统与纳米工程（英文）)
年 卷 期：2021年第7卷第2期
页 面：221-230页
核心收录：
学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 1002[医学-临床医学] 08[工学] 100214[医学-肿瘤学] 0836[工学-生物工程] 10[医学]
基 金：We are thankful for support from the Program of Shanghai Academic/Technology Research Leader(20XD1404600) Shanghai Municipal Science and Technology Commission(Grant nos.20511107600,19511107100,19511107102) Chinese Academy of Sciences(KFJ-STS-QYZD-2021-08-002) National Key Research and Development Program of China(Grant no.2016YFC0100600) Shenzhen Fundamental Research Program(JCYJ20170413164102261) Guangdong Program(2016ZT06D631)
摘 要：Prostate-specific antigen(PSA)is the most widely used biomarker for the early diagnosis of prostate *** methods for PSA detection are burdened with some limitations and require ***,we developed a novel microfluidic–electrochemical(μFEC)detection system for PSA ***,we constructed an electrochemical biosensor based on screen-printed electrodes(SPEs)with modification of gold nanoflowers(Au NFs)and DNA tetrahedron structural probes(TSPs),which showed great detection ***,we fabricated microfluidic chips by DNA TSP-Au NF-modified SPEs and a PDMS layer with designed dense meandering ***,theμFEC detection system was achieved based on microfluidic chips integrated with the liquid automatic conveying unit and electrochemical detection ***μFEC system we developed acquired great detection performance for PSA detection in PBS *** PSA assays in spiked serum samples of theμFEC system,we obtained a linear dynamic range of 1–100 ng/mL with a limit of detection of 0.2 ng/mL and a total reaction time25 *** serum samples of prostate cancer patients presented a strong correlation between the“gold-standardchemiluminescence assays and theμFEC *** terms of operation procedure,cost,and reaction time,our method was superior to the current methods for PSA detection and shows great potential for practical clinical application in the future.
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