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Remodeling of the plasma for sperm-egg recognition： proteins membrane in preparation roles of acrosomal

Asian Journal of Andrology 2015年第4期17卷 574-582页

作者： Nongnuj Tanphaichitr Kessiri Kongmanas hathairat kruevaisayawan Arpornrad Saewu Clarissa Sugeng Jason Fernandes Puneet Souda Jonathan B Angel Kym F Faull R John Aitken Julian Whitelegge Daniel Hardy Trish Berger Mark Baker Chronic Disease Program Ottawa Hospital ResearchInstitute Ottawa Ontario Canada Department ofObstetrics and Gynaecology University of OttawaOntario Canada Department of Biochemistry Microbiology and Immunology University of OttawaOntario Canada Department of Anatomy Faculty ofMedical Sciences Naresuan University PhitsanulokThailand Division of Infectious Diseases OttawaHospital - General Campus Ottawa Ontario Canada Pasarow Mass Spectrometry Laboratory Universityof California Los Angeles California USA TheARC Centre of Excellence in Biotechnology andDevelopment School of Environmental and LifeSciences University of Newcastle Callaghan NewSouth Wales Australia Department of Cell Biologyand Biochemistry Health Sciences Center Texas TechUniversity Texas USA Department of Animal ScienceUniversity of California Davis California USA

The interaction of sperm with the egg＇s extracellular matrix, the zona pellucida （ZP） is the first step of the union between male and female gametes. The molecular mechanisms of this process have been studied for the past six decades with the results obtained being both interesting and confusing. In this article, we describe our recent work, which attempts to address two lines of questions from previous studies. First, because there are numerous ZP binding proteins reported byvarious researchers, how do these proteins act together in sperm-ZP interaction？ Second, why do a number of acrosomal proteins have ZP affinity？ Are they involved mainly in the initial sperm-ZP binding or rather in anchoring acrosome reacting/reacted spermatozoa to the ZP？ Our studies reveal that a number of ZP binding proteins and chaperones, extracted from the anterior sperm head plasma membrane, coexist as high molecular weight （HMW） complexes, and that these complexes in capacitated spermatozoa have preferential ability to bind to the ZP. Zonadhesin （ZAN）, known as an acrosomal protein with ZP affinity, is one of these proteins in the HMW complexes. Immunoprecipitation indicates that ZAN interacts with other acrosomal proteins, proacrosin/acrosin and sp32 （ACRBP）, also present in the HMW complexes. Immunodetection of ZAN and proacrosin/acrosin on spermatozoa further indicates that both proteins traffic to the sperm head surface during capacitation where the sperm acrosomal matrix is still intact, and therefore they are likely involved in the initial sperm-ZP binding step.

关键词： SPERM CAPACITATION ANDSPERM-ZONA PELLUCIDAINTERACTION- BACKGROUND ANDCONFUSION IN THE FIELDS

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Inter-Professional-Compassionate pain management during endotracheal suctioning: a valuable lesson from a Chinese surgical intensive care unit

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Frontiers of Nursing 2022年第1期9卷 19-27页

作者： Qian-Wen Ruan Maneewat Khomapak hathairat Sangchan Faculty of Nursing Prince of Songkla UniversityKho HongHat YaiSongkhla 90110Thailand The Second Affiliated Hospital of Kunming Medical University KunmingYunnan 650000China

Objective:To compare the effects of a pain management program and routine suctioning methods on the level of pain presence and agitation in Chinese adults admitted to the intensive care *** disseminate the results from the implementation of the evidence-informed pain management interventions for reducing pain presence and agitation during endotracheal tube suctioning(ETS)and translate the key finding to clinical nursing ***:A quasi-experimental study of a two-group post-test design was conducted in adults admitted after surgery to a surgical intensive care unit(SICU)of the Second Affiliated Hospital of Kunming Medical University,Yunnan,China in ***-two adults who met the study eligibility were included after consent,26 in each *** in the control group received usual care while patients in the intervention group received interventions to reduce agitation and pain-related *** impacts of the intervention on the level of pain presence and agitation were measured at 5 measuring time points using the Chinese versions of Critical-Care Pain Observation Tool(CPOT)and Richmond Agitation Sedation Scale(RASS).Results:The level of pain presence in the intervention group statistically significantly decreased during,immediately after,and 5 min after *** level of agitation in the intervention group significantly decreased during and immediately after ***:The findings provide support for the positive pain-relieving effects of the evidence-informed pain-related ETS management interventions when compared with the usual ETS *** study interventions were sufficiently effective and safe to maintain patent airway clean and patent as standardized suctioning and helps pain ***,evidence-based pain-related ETS management intervention is worthy of recommending to utilize in SICU patients as well as other patients who required *** is worth noting that integrating pre-emptive analgesia prescription and a

关键词： agitation China endotracheal tube suctioning intensive care unit pain management pain presence

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Triploid Wild Rice (BKK) Strain Found in Bangkok Originated from Hybridizations among Three Parental <i>Oryza</i>Species

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American Journal of Plant Sciences 2022年第1期13卷 36-49页

作者： So Makabe Htet Aung Htut Hiroko Takahashi Sayaka Shida Masahiro Akimoto hathairat Urairong Ryuji Ishikawa Tadashi Sato Yo-Ichiro Sato Ikuo Nakamura BEX Co Ltd. Tokyo Japan Graduate School of Horticulture Chiba University Matsudo Japan Obihiro University of Agriculture and Veterinary Medicine Obihiro Japan Biotechnology Research and Development Office DOA Thanyaburi Thailand Faculty of Agriculture and Life Science Hirosaki University Hirosaki Japan Graduate School of Life Sciences Tohoku University Sendai Japan Kyoto Prefectural University Kyoto Japan

A wild rice (BKK) strain showing sterile spikelet and big leaves inhabited at the basin of the Chao Phraya river of Bangkok city, Thailand. The BKK strain was found as a natural triploid and thus its origin has been interested long time. Three different-sized fragments were amplified in RNA polymerase I largest subunit (PolA1) gene, which is a single-copy nuclear gene per haploid genome. Short type (0.14 kb) intron 20 sequence of BKK strain was identical to that of O. rufipogon and O. sativa. Phylogenetic analysis showed that long type (1.5 kb and 1.8 kb) intron 20 sequences of BKK strain were closely related to that of O. longistaminata and O. officinalis, respectively. We analyzed protein tag (Ptag) sequence encoded by exons 19 to 21 of PolA1 gene. Determined three Ptag sequences of BKK strain were identical to that of O. rufipogon, O. longistaminata, and O. officinalis, respectively. Relative DNA content of nuclei in O. officinalis and BKK strain was 1.5 and 1.75 times than that in O. sativa, respectively. And BKK strain contained CentO-C1 repeats, which were unique to O. officinalis. These results indicated that BKK strain comprised three genomes of O. rufipogon, O. longistaminata, and O. officinalis.

关键词： Oryza rufipogon Triploid Flow Cytometry PolA1 CentO-C1 Repeat Sequence

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