Remodeling of the plasma for sperm-egg recognition： proteins membrane in preparation roles of acrosomal

作     者：Nongnuj Tanphaichitr Kessiri Kongmanas Hathairat Kruevaisayawan Arpornrad Saewu Clarissa Sugeng Jason Fernandes Puneet Souda Jonathan B Angel Kym F Faull R John Aitken Julian Whitelegge Daniel Hardy Trish Berger Mark Baker 

作者机构：Chronic Disease Program Ottawa Hospital ResearchInstitute Ottawa Ontario Canada Department ofObstetrics and Gynaecology University of OttawaOntario Canada Department of BiochemistryMicrobiology and Immunology University of OttawaOntario Canada Department of Anatomy Faculty ofMedical Sciences Naresuan University PhitsanulokThailand Division of Infectious Diseases OttawaHospital - General Campus Ottawa Ontario Canada Pasarow Mass Spectrometry Laboratory Universityof California Los Angeles California USA TheARC Centre of Excellence in Biotechnology andDevelopment School of Environmental and LifeSciences University of Newcastle Callaghan NewSouth Wales Australia Department of Cell Biologyand Biochemistry Health Sciences Center Texas TechUniversity Texas USA Department of Animal ScienceUniversity of California Davis California USA 

出 版 物：《Asian Journal of Andrology》 (亚洲男性学杂志（英文版）)

年 卷 期：2015年第17卷第4期

页      面：574-582页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 0905[农学-畜牧学] 071009[理学-细胞生物学] 09[农学] 0901[农学-作物学] 090501[农学-动物遗传育种与繁殖] 090102[农学-作物遗传育种] 

基　　金：Canadian Institutes of Health Research Funding Source: Medline NIDDK NIH HHS [P30 DK063491] Funding Source: Medline NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [P30DK063491] Funding Source: NIH RePORTER 

主　　题：SPERM CAPACITATION ANDSPERM ZONA PELLUCIDAINTERACTION  BACKGROUND ANDCONFUSION IN THE FIELDS 

摘      要：The interaction of sperm with the egg＇s extracellular matrix, the zona pellucida （ZP） is the first step of the union between male and female gametes. The molecular mechanisms of this process have been studied for the past six decades with the results obtained being both interesting and confusing. In this article, we describe our recent work, which attempts to address two lines of questions from previous studies. First, because there are numerous ZP binding proteins reported byvarious researchers, how do these proteins act together in sperm-ZP interaction？ Second, why do a number of acrosomal proteins have ZP affinity？ Are they involved mainly in the initial sperm-ZP binding or rather in anchoring acrosome reacting/reacted spermatozoa to the ZP？ Our studies reveal that a number of ZP binding proteins and chaperones, extracted from the anterior sperm head plasma membrane, coexist as high molecular weight （HMW） complexes, and that these complexes in capacitated spermatozoa have preferential ability to bind to the ZP. Zonadhesin （ZAN）, known as an acrosomal protein with ZP affinity, is one of these proteins in the HMW complexes. Immunoprecipitation indicates that ZAN interacts with other acrosomal proteins, proacrosin/acrosin and sp32 （ACRBP）, also present in the HMW complexes. Immunodetection of ZAN and proacrosin/acrosin on spermatozoa further indicates that both proteins traffic to the sperm head surface during capacitation where the sperm acrosomal matrix is still intact, and therefore they are likely involved in the initial sperm-ZP binding step.