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Decoding the annulus fibrosus cell atlas by scRNA-seq to develop an inducible composite hydrogel:A novel strategy for disc reconstruction

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Bioactive Materials 2022年第8期7卷 350-363页

作者： Han Wang Di Wang Beier Luo Dong Wang haoruo jia Pandi Peng Qiliang Shang jianxin Mao Chu Gao Ye Peng Lu Gan Junjie Du Zhuojing Luo Liu Yang Department of Orthopedics Xijing HospitalFourth Military Medical UniversityXi’an710032China Department of Orthopedics Air Force Medical Center100142BeijingChina Department of Spinal Surgery Changhai HospitalNaval Medical UniversityShanghai200433China Medical Research Institute Northwestern Polytechnical UniversityXi’an710072China

Low back pain is one of the most serious public health problems worldwide and the major clinical manifestation of intervertebral disc degeneration(IVDD).The key pathological change during IVDD is dysfunction of the annulus fibrosus(AF).However,due to the lack of an in-depth understanding of AF biology,the methods to reconstruct the AF are very *** this study,the mice AF cell atlas were decoded by single-cell RNA sequencing to provide a guide for AF *** results first identify a new population of AF cells,fibrochondrocyte-like AF cells,which synthesize both collagen Ⅰ and collagen Ⅱ and are potential functional cells for AF *** to the dual features of the AF extracellular matrix,a composite hydrogel based on the acylation of methacrylated silk fibroin with methacrylated hyaluronic acid was *** obtain the ability to stimulate differentiation,the composite hydrogels were combined with a fibrochondrocyte-inducing ***,reconstruction of the AF defects,by the novel AF stem cell-loaded composite hydrogel,could be observed,its amount of chondroid matrices recovered to 31.7% of AF aera which is significantly higher than that in other control *** summary,this study decodes the AF cell atlas,based on which a novel strategy for AF reconstruction is proposed.

关键词： Single cell RNA-Seq Annulus fibrosus-derived stem cells Lineage tracing Composite hydrogel Annulus fibrosus reconstruction

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Restoring the dampened expression of the core clock molecule BMAL1 protects against compression-induced intervertebral disc degeneration

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Bone Research 2022年第2期10卷 297-309页

作者： Dong Wang Pandi Peng Michal Dudek Xueyu Hu Xiaolong Xu Qiliang Shang Di Wang haoruo jia Han Wang Bo Gao Chao Zheng jianxin Mao Chu Gao Xin He Pengzhen Cheng Huanbo Wang jianmin Zheng Judith A.Hoyland Qing-Jun Meng Zhuojing Luo Liu Yang Institute of Orthopedic Surgery Xijing HospitalFourth Military Medical UniversityXi’an 710032People’s Republic of China Medical Research Institute Northwestern Polytechnical UniversityXi’an 710068People’s Republic of China School of Biological Sciences Faculty of BiologyMedicine and HealthUniversity of ManchesterManchester M139PLUK Wellcome Centre for Cell Matrix Research University of ManchesterManchester M139PLUK Department of Medicine Chemistry and Pharmaceutical Analysis School of PharmacyFourth Military Medical UniversityXi’an 710032People’s Republic of China Radiology Department Xijing HospitalFourth Military Medical UniversityXi’an 710032People’s Republic of China

The circadian clock participates in maintaining homeostasis in peripheral tissues,including intervertebral discs(IVDs).Abnormal mechanical loading is a known risk factor for intervertebral disc degeneration(IDD).Based on the rhythmic daily loading pattern of rest and activity,we hypothesized that abnormal mechanical loading could dampen the IVD clock,contributing to ***,we investigated the effects of abnormal loading on the IVD clock and aimed to inhibit compression-induced IDD by targeting the core clock molecule brain and muscle Arnt-like protein-1(BMAL1).In this study,we showed that BMAL1 KO mice exhibit radiographic features similar to those of human IDD and that BMAL1 expression was negatively correlated with IDD severity by systematic analysis based on 149 human IVD *** intrinsic circadian clock in the IVD was dampened by excessive loading,and BMAL1 overexpression by lentivirus attenuated compression-induced *** of the RhoA/ROCK pathway by Y-27632 or melatonin attenuated the compression-induced decrease in BMAL1 ***,the two drugs partially restored BMAL1 expression and alleviated IDD in a diurnal compression *** results first show that excessive loading dampens the circadian clock of nucleus pulposus tissues via the RhoA/ROCK pathway,the inhibition of which potentially protects against compression-induced IDD by preserving BMAL1 *** findings underline the importance of the circadian clock for IVD homeostasis and provide a potentially effective therapeutic strategy for IDD.

关键词： BMAL1 degeneration homeostasis

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Extending Structures for Gel'fand-Dorfman Bialgebras

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Acta Mathematica Sinica,English Series 2024年第2期40卷 619-638页

作者： jia jia WEN Yan Yong HONG School of Mathematics Hangzhou Normal UniversityHangzhou 311121P.R.China

Gel'fand-Dorfman bialgebra,which is both a Lie algebra and a Novikov algebra with some compatibility condition,appeared in the study of Hamiltonian pairs in completely integrable *** also emerged in the description of a class special Lie conformal algebras called quadratic Lie conformal *** this paper,we investigate the extending structures problem for Gel'fand-Dorfman bialgebras,which is equivalent to some extending structures problem of quadratic Lie conformal ***,given a Gel'fand-Dorfman bialgebra(A,o,[.,.]),this problem is how to describe and classify all Gel'fand-Dorfman bialgebra structures on a vector space E(A⊂E)such that(A,o,[.,.])is a subalgebra of E up to an isomorphism whose restriction on A is the identity *** by the theories of extending structures for Lie algebras and Novikov algebras,we construct an object gH2(V,A)to answer the extending structures problem by introducing the notion of a unified product for Gel'fand-Dorfman bialgebras,where V is a complement of A in *** particular,we investigate the special case when dim(V)=1 in detail.

关键词： Gel'fand-Dorfman bialgebra Lie conformal algebra Extending structures problem Novikovalgebra

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A comprehensive study of long-term skeletal changes after spinal cord injury in adult rats

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Bone Research 2015年第3期3卷 164-172页

作者： Tiao Lin Wei Tong Abhishek Chandra Shao-Yun Hsu haoruo jia Ji Zhu Wei-Ju Tseng Michael A Levine Yejia Zhang Shi-Gui Yan X Sherry Liu Dongming Sun Wise Young Ling Qin Department of Orthopaedic Surgery Perelman School of Medicine University of Pennsylvania Department of Musculoskeletal Oncology The First Affiliated Hospital of Sun Yat-sen University Department of Orthopaedics Union Hospital Tongji Medical College Huazhong University of Science and Technology W.M.Keck Center for Collaborative Neuroscience Rutgers The State University of New Jersey Department of Orthopaedic Surgery School of Medicine Shihezi University Division of Endocrinology and Diabetes The Children’s Hospital of Philadelphia and Department of Pediatrics Perelman School of Medicine University of Pennsylvania Department of Physical Medicine & Rehabilitation Perelman School of Medicine University of Pennsylvania and Translational Musculoskeletal Research Center Philadelphia Veterans Affairs Medical Center Department of Orthopaedic Surgery 2nd Affiliated Hospital School of Medicine Zhejiang University

Spinal cord injury（SCI）-induced bone loss represents the most severe osteoporosis with no effective *** animal studies have focused primarily on long bones at the acute stage using adolescent rodents. To mimic chronic SCI in human patients, we performed a comprehensive analysis of long-term structural and mechanical changes in axial and appendicular bones in adult rats after SCI. In this experiment, 4-month-old Fischer 344 male rats received a clinically relevant T13 contusion injury. Sixteen weeks later, sublesional femurs, tibiae,and L4 vertebrae, supralesional humeri, and blood were collected from these rats and additional non-surgery rats for micro-computed tomography（m CT）, micro-finite element, histology, and serum biochemical *** trabecular sites, extreme losses of bone structure and mechanical competence were detected in the metaphysis of sublesional long bones after SCI, while the subchondral part of the same bones showed much milder damage. Marked reductions in bone mass and strength were also observed in sublesional L4 vertebrae but not in supralesional humeri. At cortical sites, SCI induced structural and strength damage in both sub- and supralesional long bones. These changes were accompanied by diminished osteoblast number and activity and increased osteoclast number and activity. Taken together, our study revealed site-specific effects of SCI on bone and demonstrated sustained inhibition of bone formation and elevation of bone resorption at the chronic stage of SCI.

关键词： SCI bone A comprehensive study of long-term skeletal changes after spinal cord injury in adult rats

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Geometric Constraints for Global Regularity of 3D Shear Thickening Fluids

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Acta Mathematicae Applicatae Sinica 2024年第1期40卷 205-210页

作者： jia-qi Yang Research&Development Institute of Northwestern Polytechnical University in Shenzhen Shenzhen518063China Key Laboratory for Mechanics in Fluid Solid Coupling Systems Institute of MechanicsChinese Academy of SciencesBeijing100190China

We consider the relation between the direction of the vorticity and the global regularity of 3D shear thickening *** is showed that a weak solution to the non-Newtonian incompressible fluid in the whole space is stron... 详细信息

关键词： direction of the vorticity non-Newtonian fluid global regularity

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