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A genome-wide association study on growth traits in orangespotted grouper(Epinephelus coioides) with RAD-seq genotyping

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Science China(Life Sciences) 2018年第8期61卷 934-946页

作者： Hui Yu Xinxin You Jia Li Xinhui Zhang Shuai Zhang Shoujia Jiang Xueqiang lin hao-ran lin Zining Meng Qiong Shi BGI Education Center University of Chinese Academy of Sciences Shenzhen Key Lab of Marine Genomics Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals BGI Academy of Marine SciencesBGI Marine BGI State Key Laboratory of Biocontrol Institute of Aquatic Economic Animals and the Guangdong Province Key Laboratory for Aquatic Economic Animals Sun Yat-Sen University Laboratory of Aquatic Genomics College of Life Sciences and Oceanography Shenzhen University

The orange-spotted grouper, Epinephelus coioides, is one of the most popular fish in China and Southeast Asian countries because of its important economic value. However, molecular mechanism underlying the growth of orange-spotted grouper has never been fully understood. Herein, we performed a genome-wide association study （GWAS） on a natural population of 198 individuals aiming to screen the whole genome of orange-spotted grouper for identification of growth-related loci by restriction-site associated DNA sequencing. In this research, 261,366 single nucleotide polymorphisms （SNPs） were developed, in which 110 SNPs were identified to be correlated with growth and 20 SNPs were further confirmed to be associated with both body weight and total length. From these identified SNPs, we annotated a total of 34 genes, including adgrb2, csnkzal, cers5, co122al, creb5, dndl, dzankl, dnail, npy2r, fat3, lrrk2, lrp5, map3k9, and so on. Among these candidate genes, npy2r （neuropeptide Y receptor Y2） was reported to play a critical role in growth of the orange-spotted grouper. In addition, population structure, principal component analysis, kinship matrix and linkage disequilibrium were examined to verify the accuracy and reliability of our GWAS results. Our data will also provide a valuable genetic resource for further marker-assisted selection program to improve growth quality in groupers.

关键词： orange-spotted grouper genome-wide association study (GWAS) restriction-site associated DNA sequencing (RAD-scq) growth traits npy2r

First identification of two co-existing genome-wide significant sex quantitative trait loci(QTL) in red tilapia using integrative QTL mapping

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Zoological Research 2022年第2期43卷 205-216页

作者： Zong-Xian Zhu Yi-Long lin Chun-Hui Ai Ying-Ying Xiong Dan-Dan Huang Yin-Yi Yao Tong-De Liu Chao-hao Chen hao-ran lin Jun-Hong Xia College of Life Sciences State Key Laboratory of BiocontrolInstitute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic AnimalsSun Yat-Sen UniversityGuangzhouGuangdong 510275China College of Traditional Chinese Medicine Guangdong Pharmaceutical UniversityGuangzhouGuangdong 510006China Maoming Branch Guangdong Laboratory for Lingnan Modern AgricultureMaomingGuangdong 525000China

Red tilapia(Oreochromis spp.)is one of the most popular fish in China due to its bright red appearance,fast growth rate,and strong *** the sex determination mechanisms is of vital importance for the selection of all-male lines to increase aquacultural production of red *** this research,the genetic architecture for sex from four mapping populations(n=1090)of red tilapia was analyzed by quantitative trait loci(QTL)-seq,linkage-based QTL mapping,and linkage disequilibrium(LD)-based genome-wide association *** genome-wide significant QTL intervals associated with sex were identified on ChrLG1(22.4-23.9 Mb)and ChrLG23(32.0-35.9 Mb),*** QTL on ChrLG1 was detected in family 1(FAM1),FAM2,and FAM4,and the other QTL on ChrLG23 was detected in FAM3 and *** microsatellite markers located within the QTL were successfully developed for marker-assisted ***,three(Ipp,sox14,and amh)of the 12 candidate genes located near or on the two QTL intervals were abundantly expressed in males,while the remaining genes were more highly expressed in *** genes(scly,ube3a,Ipp,gpr17,oca2,cog4,and atp10a)were significantly differentially expressed between the male and female ***,LD block analysis suggested that a cluster of genes on ChrLG23 may participate in regulating sex development in red *** study provides important information on the genetic architecture of sex in red tilapia and should facilitate further exploration of sex determination mechanisms in this species.

关键词： Red tilapia QTL mapping Sex SNPs

Whole-genome sequencing of leopard coral grouper(Plectropomus leopardus) and exploration of regulation mechanism of skin color and adaptive evolution

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Zoological Research 2020年第3期41卷 328-340页

作者： Yang Yang Li-Na Wu Jing-Fang Chen Xi Wu Jun-Hong Xia Zi-Ning Meng Xiao-Chun Liu hao-ran lin State Key Laboratory of Biocontrol Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic AnimalsLife Sciences SchoolSun Yat-Sen UniversityGuangzhouGuangdong 510275China State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources School of Life SciencesSun Yat-Sen UniversityGuangzhouGuangdong 510275China Southern Laboratory of Ocean Science and Engineering ZhuhaiGuangdong 519000China

Leopard coral groupers belong to the Plectropomus genus of the Epinephelidae family and are important fish for coral reef ecosystems and the marine aquaculture industry. To promote future research of this species, a high-quality chromosome-level genome was assembled using PacBio sequencing and Hi-C technology. A 787.06 Mb genome was assembled, with 99.7%(784.57 Mb) of bases anchored to 24 chromosomes. The leopard coral grouper genome size was smaller than that of other groupers, which may be related to its ancient status among grouper species. A total of 22 317 proteincoding genes were predicted. This high-quality genome of the leopard coral grouper is the first genomic resource for Plectropomus and should provide a pivotal genetic foundation for further research. Phylogenetic analysis of the leopard coral grouper and 12 other fish species showed that this fish is closely related to the brown-marbled *** genes in the leopard coral grouper genome were mainly associated with immune response and movement ability, which may be related to the adaptive evolution of this species to its habitat. In addition, we also identified differentially expressed genes(DEGs) associated with carotenoid metabolism between red and brown-colored leopard coral groupers. These genes may play roles in skin color decision by regulating carotenoid content in these groupers.

关键词： Leopard coral grouper Genome Evolution Skin color Immune

Role of epithelial-to-mesenchymal transition in the pulmonary fi brosis induced by paraquat in rats

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World Journal of Emergency Medicine 2021年第3期12卷 214-220页

作者： Jian-hua Yi Zhao-cai Zhang Mei-bian Zhang Xin He hao-ran lin Hai-wen Huang Hai-bin Dai Yu-wen Huang Emergency Department the Second Affi liated Hospital of Zhejiang University School of MedicineHangzhou 310009China Scientifi c Research Department the Second Affi liated Hospital of Zhejiang University School of MedicineHangzhou 310009China Zhejiang Provincial Center for Disease Control and Prevention Hangzhou 301051China Shangyu People’s Hospital Shaoxing 312300China Pharmacy Department the Second Affi liated Hospital of Zhejiang University School of MedicineHangzhou 310009China

BACKGROUND:This study aims to explore the characteristics of the epithelial-to-mesenchymal transition(EMT)process and its underlying molecular mechanisms in the period of paraquat(PQ)-induced pulmonary fi brosis(PF).METHODS:Picrosirius red staining and collagen volume fraction were utilized to evaluate the pathological changes of PQ-induced PF in ***,Western blot,and real-time reverse transcriptase-polymerase chain reaction(RT-PCR)were used to measure the protein and gene expression of EMT markers,EMT-associated transcription factors,and regulators of EMT-related pathways,***:The collagen deposition in the alveolar septum and increased PF markers were characteristics of pathological changes in PQ-induced PF,reached a peak on day 14 after PQ poisoning,and then decreased on day *** protein and gene expression of the fibrosis marker,EMT markers,transcription factors,and regulators of EMT-related signaling pathways signifi cantly increased at diff erent time points after PQ poisoning compared with corresponding controls(Pranscription factors,and regulators of EMT-related signaling pathways(Pranscription factors was signifi cantly correlated with that of TGF-β1 and Smad2(P0.05).CONCLUSIONS:EMT process plays a role in the PQ-induced PF,in which most PF and EMT markers have a peak phenomenon,and its underlying molecular mechanisms might be determined by further studies.

关键词： Pulmonary fibrosis Paraquat Epithelial-to-mesenchymal transition Rats

Molecular Cloning and mRNA Profile of Insulin-like Growth Factor Type 1 Receptor in Orange-spotted Grouper,Epinephelus coioides

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Acta Biochimica et Biophysica Sinica 2005年第5期37卷 327-334页

作者： Yue-Mei KUANG Wen-Sheng LI hao-ran lin Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals Zhongshan University, Guangzhou 510275, China Ins.ofAquaticEconomicAnimalsandGuangdongProvincialKeyLaboratoryforAquaticEconomicAnimals ZhongshanUniversit

The insulin-like growth factor type 1 receptor(IGF-IR)belongs to the tyrosine kinase(TK)receptor *** being mitogenic,IGF-IR plays a crucial role in cell survival,transformation andmaintenance of the malignant *** this study,we cloned the cDNA from the hypothalamus of theorange-spotted grouper(Epinephelus coioides)using reverse transcription PCR(RT-PCR)and the rapidamplification of cDNA ends(RACE)*** deduced amino acid sequence showed that the receptorcomprises 1413 amino acid *** contains cysteine-rich domains in its α-subunit,and a conservedtransmembrane domain and TK domains in its β-*** of the amino acid sequence withthose of other species showed that the grouper IGF-IR shares 90.2%,89.6%,71.9% and 72% similaritywith the IGF-IR of the Japanese flounder,turbot,zebrafish-a and zebrafish-b,*** comparedwith its mammalian homologue,grouper IGF-IR contains a large insertion at its *** has revealed that the grouper IGF-IR belongs to the b-type IGF-IRs and has a higher similarity withflounder and turbot IGF-IR,and a lower similarity(ranscripts were detected in the brain,peripheral tissues,embryos and early development larvae bysemi-quantitative RT-PCR *** was observed that IGF-IR mRNA expression was greater in the brainthan in peripheral *** level of IGF-IR mRNA expression was much higher in retina,gonad,skeletalmuscle and gill tissues than in liver,heart and thymus *** expression of IGF-IR can be visualizedas a ubiquitous signal in unfertilized eggs,embryos and early development *** distribution pattern ofIGF-IR mRNA in grouper development suggests that IGF-IR plays an important role in the embryo andearly larval development stages.

关键词： orange-spotted grouper(Epinephelus coioides) insulin-like growth factor type 1 receptor cDNA cloning mRNA expression

The contribution of the new Ⅲ antiarrhythmic agent Nifekalant to the delayed rectifier potassuim current and calcium current of the ventricular myocyte of rat

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The contribution of the new Ⅲ antiarrhythmic agent Nifekala...

NOX4 exacerbates Parkinson's disease pathology by promoting neuronal ferroptosis and neuroinflammation

第十三次全国心血管病学术会议

作者： LI rang,DING Yansheng,HUO Yong,CAI hao-ran,LI lin Department of Cardiology,Peking University First Hospital,Beijing 100034,China

Objective The delayed rectifier potassuim current(I) is the main repolarizing outward current of cardiac action potential (AP).Its major contributions to the cardiac AP are control of stable resting membrane potential and termination of the AP,thus play a prominent role in *** new Ⅲ antiarrhythmic agent"Nifekalant"is an unselective I blocker,which is regarded as one of the recommendatory drugs for refractory ventricular arrhythmias in Asia(most in

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Neural Regeneration Research 2025年第7期20卷 2038-2052页

作者： Zhihao lin Changzhou Ying Xiaoli Si Naijia Xue Yi Liu ran Zheng Ying Chen Jiali Pu Baorong Zhang Department of Neurology The Second Affiliated HospitalZhejiang University School of MedicineHangzhouZhejiang ProvinceChina

Parkinson's disease is primarily caused by the loss of dopaminergic neurons in the substantia nigra ***,a novel form of regulated cell death characterized by iron accumulation and lipid peroxidation,plays a vital role in the death of dopaminergic ***,the molecular mechanisms underlying ferroptosis in dopaminergic neurons have not yet been completely *** oxidase 4 is related to oxidative stress,however,whether it regulates dopaminergic neuronal ferroptosis remains *** aim of this study was to determine whether NADPH oxidase 4 is involved in dopaminergic neuronal ferroptosis,and if so,by what *** found that the transcriptional regulator activating transcription factor 3 increased NADPH oxidase 4 expression in dopaminergic neurons and astrocytes in an 1-methyl-4-phenyl-1,2,3,6 tetrahydropyridine-induced Parkinson's disease *** oxidase 4 inhibition improved the behavioral impairments observed in the Parkinson's disease model animals and reduced the death of dopaminergic ***,NADPH oxidase 4 inhibition reduced lipid peroxidation and iron accumulation in the substantia nigra of the Parkinson's disease model ***,we found that NADPH oxidase 4 interacted with activated protein kinase Cαto prevent ferroptosis of dopaminergic ***,by lowering the astrocytic lipocalin-2 expression,NADPH oxidase 4 inhibition reduced 1-methyl-4-phenyl-1,2,3,6 tetrahydropyridine-induced *** findings demonstrate that NADPH oxidase 4 promotes ferroptosis of dopaminergic neurons and neuroinflammation,which contribute to dopaminergic neuron death,suggesting that NADPH oxidase 4 is a possible therapeutic target for Parkinson's disease.

关键词： dopaminergic neuron ferroptosis NADPH oxidase 4(NOX4) neuroinflammation Parkinson's disease

Marker-assisted selection of YY supermales from a genetically improved farmed tilapia-derived strain

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固相增密燃料元件基体石墨的氩离子辐照性能研究

动物学研究 2019年第2期

作者： Chao-hao Chen Bi-Jun Li Xiao-Hui Gu hao-ran lin Jun-Hong Xia State Key Laboratory of Biocontrol， Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals， College of Life Sciences， Sun Yat-Sen University， Guangzhou Guangdong 510275， China

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新型炭材料 2018年第3期33卷 268-275页

作者：许六军王浩然林俊许红霞张锋仲亚娟朱智勇郭全贵中国科学院上海应用物理研究所上海201800 中国科学院大学北京100049 中国科学院山西煤炭化学研究所中国科学院炭材料重点实验室山西太原030001

为提高熔盐堆燃料元件基体石墨的致密性,采用准等静压工艺,对球形燃料元件A3-3基体石墨进行中间相炭微球(MCMB)固相增密,并对其进行了氩离子辐照性能研究。通过压汞测试研究了增密后基体石墨的进汞临界压强和平均孔径变化;利用氩离子辐... 详细信息

为提高熔盐堆燃料元件基体石墨的致密性,采用准等静压工艺,对球形燃料元件A3-3基体石墨进行中间相炭微球(MCMB)固相增密,并对其进行了氩离子辐照性能研究。通过压汞测试研究了增密后基体石墨的进汞临界压强和平均孔径变化;利用氩离子辐照实验并结合纳米压痕技术和拉曼光谱分析,研究了固相增密前后基体石墨的离子辐照效应。结果表明,采用平均粒径为2、10和16μm的MCMB对基体石墨A3-3增密后,基体石墨孔径由924 nm分别降至530、573和644 nm,MCMB以其自烧结同步收缩的特性,起到了填充孔隙和裂缝的作用,实现了对基体石墨的固相增密。MCMB增密剂粒径越接近基体石墨的孔径,增密后基体石墨的平均孔径越小,进汞临界压强越高,即增密效果越好。样品纳米压痕的测试表明高剂量离子辐照下(>1 dpa),MDG2-15(粒径2μm,质量百分数为15%的MCMB增密基体石墨)石墨比A3-3石墨的离子辐照硬化速率慢。拉曼光谱结果显示增密前后基体石墨在1.47 dpa剂量下,均发生非晶化,致密化调控前后基体石墨的离子辐照效应表现出一致性。

关键词：熔盐堆燃料元件基体石墨离子辐照纳米压痕