检索结果-南通市图书馆

Extracellular vesicles derived from mesenchymal stem cells mediate extracellular matrix remodeling in osteoarthritis through the transport of microRNA-29a

引用

World Journal of Stem Cells 2024年第2期16卷 191-206页

作者： Fan Yang wan-Qi Xiong Chen-Zhi Li Ming-Jian Wu Xiu-Zhi Zhang Chun-Xiao Ran Zhen-hao Li Yan Cui Bao-Yi Liu De-Wei Zhao Department of Orthopedics Affiliated Zhongshan Hospital of Dalian UniversityDalian 116001Liaoning ProvinceChina

BACKGROUND Knee osteoarthritis(KOA)is a common orthopedic condition with an uncertain etiology,possibly involving genetics and biomechanics.Factors like changes in chondrocyte microenvironment,oxidative stress,inflammation,and immune responses affect KOA development.Early-stage treatment options primarily target symptom relief.Mesenchymal stem cells(MSCs)show promise for treatment,despite challenges.Recent research highlights microRNAs(miRNAs)within MSC-released extracellular vesicles that can potentially promote cartilage regeneration and hinder KOA progression.This suggests exosomes(Exos)as a promising avenue for future treatment.While these findings emphasize the need for effective KOA progression management,further safety and efficacy validation for Exos is essential.AIM To explore miR-29a’s role in KOA,we’ll create miR-29a-loaded vesicles,testing for early treatment in rat models.METHODS Extraction of bone marrow MSC-derived extracellular vesicles,preparation of engineered vesicles loaded with miR-29a using ultrasonication,and identification using quantitative reverse transcription polymerase chain reaction;after establi-shing a rat model of KOA,rats were randomly divided into three groups:Blank control group injected with saline,normal extracellular vesicle group injected with normal extracellular vesicle suspension,and engineered extrace-llular vesicle group injected with engineered extracellular vesicle suspension.The three groups evaluation,histological detection,and immunohistochemical detection to compare and evaluate the progress of various forms of arthritis.RESULTS General behavioral observation results showed that the extracellular vesicle group and engineered extracellular vesicle group had better performance in all four indicators of pain,gait,joint mobility,and swelling compared to the blank control group.Additionally,the engineered extracellular vesicle group had better pain relief at 4 wk and better knee joint mobility at 8 wk compared to the normal extracellular vesicle group.Imaging examinat

关键词： Exosomes Osteoarthritis Mesenchymal stem cells MicroRNA-29a Intra-articular injection

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Rituximab induction therapy in highly sensitized kidney transplant recipients

引用

Chinese Medical Journal 2011年第13期124卷 1928-1932页

作者： YIN Hang wan hao HU Xiao-peng LI Xiao-bei wanG Wei LIU Hang REN Liang ZHANG Xiao-dong Kidney Transplantation Program Department of Urology BeijingChao-Yang Hospital Capital Medical University Beijing 100020China

Background The number of highly sensitized patients is rising, The present study aimed to investigate the safety and efficacy of rituximab in highly sensitized kidney transplant recipients. and sensitization can lead to renal transplant failure. renal transplantation following induction therapy with Methods Seven highly sensitized kidney transplant recipients who underwent rituximab therapy from December 2008 to December 2009 were retrospectively analyzed. There were 3 men and 4 women, with a mean age of 38.5 years （range, 21-47 years）. The duration of hemodialysis was 3-12 months, with a mean duration of 11 months. For 4 patients, this was the second transplant; the previous graft survival time was 2-11 years, with a mean survival time of 5.8 years. All the female recipients had history of multiple pregnancies, and all patients had previously received blood transfusions. All donors were men, with a mean age of 32.5 years （range, 25-37 years）. In 2 of the 7 patients, both class I and class II of panel reactive antibody were high; the remaining 5 patients showed either high in class I or in class II of panel reactive antibody. The mean panel reactive antibody value was 31% for class I and 51% for class II respectively. The donors and the recipients had the same blood type, with low lymphocyte cytotoxicity ranging from 2% to 5%. The human leukocyte antigen （HLA） mismatch numbers were from 2 to 4. All patients received tacrolimus （0.1 mg.kg^-1 .d^-1） and mycophenolate mofetil （750 mg twice per day） orally 3 days prior to surgery. All patients received a single dose of 600 mg rituximab （375 mg/m^2） infusion on the day before surgery and polyclonal antibody （antithymocyte globulin） on the day of surgery. Postoperative creatinine, creatinine clearance rate, and occurrence of rejection by pathological biopsy confirmation were monitored. Results No patient had delayed graft function after surgery. Two patients had acute rejection, one on day 7 and the other on day 13 post-surgery. Diagnosis of acute rejections was based

关键词： kidney transplant highly sensitized rituximab

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The prognostic and immunological impacts of DCX expression:a pan-cancer analysis

引用

Cancer Advances 2024年第14期7卷 1-8页

作者： Xin Li wan-Rong Li hao Jin Tianjin Cancer Hospital Airport Hospital Tianjin 300060China Tianjin Medical University Cancer Institute and Hospital National Clinical Research Center for CancerKey Laboratory of Cancer Prevention and TherapyTianjin’s Clinical Research Center for CancerTianjin 300060China.

Background:Doublecortin(DCX),a microtubule-associated protein,is best known for its critical role in neuronal migration during neural development,where it stabilizes microtubules and guides neurons to their proper positions.Recently,DCX has been implicated in various cancer processes,suggesting it may influence tumor progression and the tumor microenvironment.Emerging evidence indicates that DCX can modulate cell migration,invasion,and interaction with immune cells,making it a potential player in oncogenesis.However,the role of DCX across different cancer types and its potential as a prognostic biomarker remain underexplored,necessitating a comprehensive analysis.Methods:We utilized The Cancer Genome Atlas to extract data on DCX expression in tumor and adjacent normal tissues across diverse cancer types.Differential expression analysis was conducted using differential expression sequencing 2.Survival analysis was performed with Kaplan-Meier estimates and Cox proportional hazards models.Correlations between DCX expression and tumor mutational burden,microsatellite instability,and immune infiltration were examined using Spearman’s correlation.Results:DCX showed variable expression across cancer types,with significant overexpression in certain tumors such as liver and lung cancer and downexpression in others like breast cancer.High DCX expression was correlated with poor prognosis in adrenocortical carcinoma but with better outcomes in low-grade glioma.Additionally,DCX expression was significantly associated with various immune markers and chemokines,suggesting a role in modulating the immune microenvironment.Conclusion:Our findings highlight the complex role of DCX in cancer,underlining its potential as a prognostic marker and its involvement in immune-related pathways.Targeting DCX could represent a novel approach to modulating tumor behavior and enhancing immune response in cancer therapy.

关键词： pan-cancer analysis tumor microenvironment prognostic biomarker immune infiltration chemokine signaling cancer genomics

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Genetic differentiation of Bemisia tabaci （Gennadius）（Hemiptera： Aleyrodidae） biotype Q based on mitochondrial DNA markers

引用

Insect Science 2008年第2期15卷 115-123页

作者： Dong Chu Fang-hao wan Yun-Li Tao Guo-Xia Liu Zhong-Xue Fan Yu-Ping Bi High-tech Research Center Shandong Academy of Agncultural Sciences Jinan Shandong The State Key Laboratory for Biology ofPlant Diseasesand Insect Pests Institute of Plant Protection Chinese Academy of Agncultural Sciences Beijing China

In the present study, genetic differentiation of Bemisia tabaci （Gennadius） biotype Q was analyzed based on mitochondrial cytochrome oxidase I （mt CO1） gene sequence. The results showed that B. tabaci biotype Q could be separated into two subclades, which were labeled as subclades Q1 and Q2. Subclade Q1 was probably indigenous to the regions around the Mediterranean area and subclade Q2 to Israel or Cyprus. It was because B. tabaci was composed of several genetically distinct groups with a strong geographical association between more closely related biotypes. Not all of the B. tabaci biotype Q in the non-Mediterranean countries come from the same regions. Until now, all B. tabaci biotype Q in China were grouped into subclade Q 1. The B. tabaci biotype Q introduced into the US included both subclades Q 1 and Q2. The genetic structure analysis showed higher genetic variation of subclade Q 1 than that of subclade Q2.

关键词： Bemisia tabaci biotype Q genetic differentiation mitochondria COI gene

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Direct constraint on cosmic neutrino mass using observational Hubble parameter data

引用

Research in Astronomy and Astrophysics 2018年第10期18卷 75-84页

作者： hao-Yi wan Shu-Lei Cao Huan-Yu Teng Yu Liu Jian-Chen Zhang Tong-Jie Zhang Department of Astronomy Beijing Normal UniversityBeijing 100875China Dezhou University Dezhou 253023China

In order to explore the properties of cosmic neutrinos, i.e. sum of the neutrino mass （∑mv） and the effective number of neutrino species （Neff）, which affects the Hubble expansion rate H（z） and the power of observational Hubble parameter data （OHD） in constraining cosmological parameters under the ACDM model, we utilize OHD to constrain the properties of cosmic neutrinos and apply an accurate H（z） function with ∑ mv, and Neff. First, we simulate new OHD beyond the existing 43 OHD. According to the predictions of measurements of Ho （the current H（z） value）, baryon acoustic oscillations （BAO） peaks, Sandage-Loeb （SL） test and cosmic microwave background （CMB）, we as-sume observational accuracy up to 2% and redshift 0 〈 z ~〈 5. With simulated H（z） data obtained from the fiducial model, we constrain the parameters including ∑ mv, and Neff. When all parameters are set free, ∑mv 〈 0.196eV （95%） and Neff = 2.984 ± 0.826 （68%） are obtained, and when fixing Neff as the standard baseline 3.046, we attain ∑ mv 〈 0.240 eV （95%）. These constrained results are much tighter than the ones obtained by the current OHD, which makes the prospect of OHD in constraining cosmological parameters more promising as its accuracy and quantity grow.

关键词： cosmological parameters - cosmology miscellaneous - methods data analysis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Potential geographical distribution of Rhagoletis pomonella （Diptera： Tephritidae）in China

引用

Insect Science 2011年第5期18卷 575-582页

作者： Jian Geng Zhi-Hong Li Edwin G. Rajotte Fang-hao wan Xiao-Yu Lu Zhi-Ling wang Department of Entomology College of Agronomy and Biotechnology China Agricultural University Beijing Gaoming Entry-Exit Inspection and Quarantine Bureau Foshan Guangdong Province China College of Agricultural Sciences The Pennsylvania State University State College Pennsylvania USA Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing Beijing Academy of Agriculture and Forestry Sciences Beijing China

Apple maggot fly, Rhagoletis pomonella （Walsh） is a major pest causing considerable economic losses of fruits in North America. During the development of international trade, apple maggot fly has become a threat to Chinese agriculture. In this study, CLIMEX and ArcGIS were used to predict the potential geographical distribution of apple maggot fly in China. The parameters used in CLIMEX for apple maggot fly were derived from ecological data and the present geographical distribution of apple maggot fly in North America. Then the potential distribution map in China was presented based on the adjusted values of these parameters. The results showed that apple maggot fly has a wide potential distribution area in China; 47.5% of 748 meteorological stations presented high or medium suitability of pest establishment. These high suitable stations are mainly located in northeast, southwest and northwest of China, such as Liaoning, Shandong, Gansu and Shaanxi Provinces. These areas are also the central regions of apple, pear and peach production in China. Two hundred and twenty-five stations （30.1%） in westem and southern China, such as Tibet, Qinghai, Guangdong, Guangxi, Hainan and Taiwan, were unsuitable for establishment of apple maggot fly populations. In order to prevent the introduction of apple maggot fly in China, the present plant quarantine measures should be enhanced, especially in the areas with high suitability for the presence of apple maggot fly.

关键词： apple maggot fly ArcGIS CLIMEX potential geographical distribution Rhagoletis pomonella （Walsh）

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

布尔台煤矿综放工作面矿压显现规律研究

引用

煤炭工程 2022年第12期54卷 78-83页

作者：郝万东中国煤炭科工集团太原研究院有限公司山西太原030006

针对神东矿区特大型矿井放顶煤开采矿压监测研究不足的问题,以布尔台煤矿放顶煤工作面为背景,对该条件下的矿压显现规律进行了现场实测研究。巷道变形监测显示,辅运巷顶板位移累计下沉量为100~130mm,正帮帮鼓量150mm,副帮帮鼓量累计100m... 详细信息

针对神东矿区特大型矿井放顶煤开采矿压监测研究不足的问题,以布尔台煤矿放顶煤工作面为背景,对该条件下的矿压显现规律进行了现场实测研究。巷道变形监测显示,辅运巷顶板位移累计下沉量为100~130mm,正帮帮鼓量150mm,副帮帮鼓量累计100m,顶板离层最大为5mm,巷道变形程度可控;围岩应力结果显示,超前支承压力影响范围大于75m,须严格执行超前锚杆索支护;工作面液压支架受力监测结果显示,初次来压步距为71.3m,来压持续长度为16.9m,周期来压平均步距为14.8m,来压距离约为9m;微震监测结果显示,微震事件多聚集在机头和机尾附近,微震大能量事件聚集在侧向采空区煤柱附近,工作面推进速度越快,微震总能量越大,保持工作面匀速开采有利于工作面矿压治理。该研究结果可为高产高效放顶煤开采矿井矿压防治提供借鉴参考。

关键词：神东矿区综放工作面矿压显现微震

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

次乌头碱配伍甘草次酸对缺氧缺糖损伤H9c2心肌细胞的保护作用及其分子机制（英文）

引用

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2017年第7期18卷 586-596页

作者： Li-qin wanG Yu HE hao-fang wan Hui-fen ZHOU Jie-hong YANG Hai-tong wan Cardio-Cerebro Vascular Research Institute Zhejiang Chinese Medical University Hangzhou 310053 China

目的:探讨次乌头碱(HA)配伍甘草次酸(GA)对缺氧缺糖(OGD)损伤H9c2心肌细胞的保护作用及其作用机制。创新点:首次在OGD模型中证明HA+GA对H9c2心肌细胞有明显的保护作用。此作用与减少细胞坏死和凋亡有关系,且其作用机制与磷脂酰肌醇-3-激... 详细信息

目的:探讨次乌头碱(HA)配伍甘草次酸(GA)对缺氧缺糖(OGD)损伤H9c2心肌细胞的保护作用及其作用机制。创新点:首次在OGD模型中证明HA+GA对H9c2心肌细胞有明显的保护作用。此作用与减少细胞坏死和凋亡有关系,且其作用机制与磷脂酰肌醇-3-激酶/蛋白质丝氨酸-苏氨酸激酶(PI3K/Akt)信号通路有关。方法:采用H9c2心肌细胞为研究对象,将其分为七组:正常组、OGD模型组、OGD+HA组、OGD+GA组、OGD+HA+GA组、OGD+LY294002组、OGD+HA+GA+LY294002组。采用Hoechst 33342染色荧光显微镜及透射电镜观察前五组的H9c2心肌细胞的形态学改变;采用酶联免疫吸附测定法(ELISA)检测前五组细胞上清液中乳酸脱氢酶(LDH)、肌酸激酶同工酶(CK-MB)以及天门冬氨酸氨基转移酶(AST)的释放量的改变;采用异硫氰酸荧光素-磷脂结合蛋白V/碘化丙啶(FITC-AV/PI)双染色法检测前五组细胞凋亡率的情况;采用蛋白质免疫印迹法(Western blot)检测加入抑制剂LY294002前后丝苏氨酸蛋白激酶(Akt)、磷酸化丝苏氨酸蛋白激酶(p-Akt)、B细胞淋巴瘤/白血病-2相关x蛋白(Bax)、B细胞淋巴瘤/白血病-2(Bcl-2)及半胱氨酸天冬氨酸蛋白酶-9(caspase-9)等细胞作用信号通路PI3K/Akt相关蛋白的情况。结论:(1)Hoechst 33342染色荧光显微镜显示OGD+HA+GA组抗凋亡作用最明显;(2)透射电镜观察OGD+HA+GA组凋亡现象改善最多;(3)LDH、CK-MB及AST的含量变化显示OGD+HA+GA组心肌细胞损伤指标降低最多(P<0.05);(4)Western blot法检测结果显示HA+GA可以减少OGD对H9c2心肌细胞的损伤,其作用机制与PI3K/Akt信号通路有关。

关键词：次乌头碱甘草次酸 H9c2心肌细胞凋亡 PI3K/Akt

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Synthesis,characterization,and tunable wettability of poly (ionic liquid) brushes via nitroxide-mediated radical polymerization (NMP)

引用

Chinese Science Bulletin 2010年第31期55卷 3562-3568页

作者： YAG Wu HE XiaoJing GAO JinZhang GUO hao HE XiaoYan wan Fei Zhao XiuLi YU Yan PEI Ben College of Chemistry and Chemical Engineering Northwest Normal University Lanzhou 730070 China

Well-defined poly(ionic liquid) brushes with tunable wettability are successfully synthesized via nitroxide-mediated radical polymerization.X-ray photoelectron spectroscopy,attenuated total reflection infrared spectroscopy and static water contact angle measurements are used to monitor each step.Ellipsometry,nuclear magnetic resonance(1H and 13C) spectra and gel permeation chromatographic measurements reveal that there is a linear increase in polymer film thickness with polymerization time,indicating that chain growth from the surface is a controlled/"living" polymerization.The surface morphology was measured by atomic force microscopy.Moreover,the surfaces of the poly(ionic liquid) brushes with tunable wettability and reversible switching between hydrophilicity and hydrophobicity can be easily achieved by counteranion exchange.

关键词：自由基聚合离子液体润湿性可调谐合成 NMP 表面形貌测量 X射线光电子能谱

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Design and prototyping of the readout electronics for the transition radiation detector in the high energy cosmic radiation detection facility

引用

Nuclear Science and Techniques 2024年第4期35卷 189-199页

作者： Jie-Yu Zhu Yang-Zhou Su Hai-Bo Yang Fen-Hua Lu Yan Yang Xi-Wen Liu Ping Wei Shu-Cai wan hao-Qing Xie Xian-Qin Li Cong Dai Hui-Jun Hu Hong-Bang Liu Shu-Wen Tang Cheng-Xin Zhao Institute of Modern Physics Chinese Academy of SciencesLanzhou 730000China School of Nuclear Science and Technology University of Chinese Academy of SciencesBeijing 100049China Guangxi Key Laboratory for Relativistic Astrophysics School of Physical Science and TechnologyGuangxi UniversityNanning 530004China

The high energy cosmic-radiation detection(HERD)facility is planned to launch in 2027 and scheduled to be installed on the China Space Station.It serves as a dark matter particle detector,a cosmic ray instrument,and an observatory for high-energy gamma rays.A transition radiation detector placed on one of its lateral sides serves dual purpose,(ⅰ)calibrating HERD's electromagnetic calorimeter in the TeV energy range,and(ⅱ)serving as an independent detector for high-energy gamma rays.In this paper,the prototype readout electronics design of the transition radiation detector is demonstrated,which aims to accurately measure the charge of the anodes using the SAMPA application specific integrated circuit chip.The electronic performance of the prototype system is evaluated in terms of noise,linearity,and resolution.Through the presented design,each electronic channel can achieve a dynamic range of 0–100 fC,the RMS noise level not exceeding 0.15 fC,and the integral nonlinearity was<0.2%.To further verify the readout electronic performance,a joint test with the detector was carried out,and the results show that the prototype system can satisfy the requirements of the detector's scientific goals.

关键词： HERD Dark matter particle detection TRD Readout electronics SAMPA Data acquisition Performance test

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：