Protective mechanisms of hypaconitine and glycyrrhetinic acid compatibility in oxygen and glucose deprivation injury

作     者：Li-qin WANG Yu HE Hao-fang WAN Hui-fen ZHOU Jie-hong YANG Hai-tong WAN 

作者机构：Cardio-Cerebro Vascular Research Institute Zhejiang Chinese Medical University Hangzhou 310053 China 

出 版 物：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 (浙江大学学报（英文版）B辑（生物医学与生物技术）)

年 卷 期：2017年第18卷第7期

页      面：586-596页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Project supported by the National Natural Science Foundation of China(Nos.81473412,81573868,and 81630105) the Zhejiang Provincial Natural Science Foundation of China(No.LZ17H270001) the Zhejiang Provincial Program for the Cultivation of High-Level Innovative Health Talents,China 

主　　题：Hypaconitine (HA) Glycyrrhetinic acid (GA) H9c2 cells Apoptosis PI3K/Akt 

摘      要：This study investigated the protective effect of the compatibility of hypaconitine （HA） and glycyrrhetinic acid （GA） on H9c2 cells under oxygen and glucose deprivation （OGD）-induced injury, and the possible mechanisms. We found that HA＋GA significantly improved pathology and morphology of the nucleus and ultrastructure of H9c2 cells under OGD as determined by Hoechst 33342 staining and transmission electron microscopy （TEM） tests. It also reduced the releases of lactate dehydrogenase （LDH）, creatine kinase-myocardial band isoenzyme （CK-MB）, and aspartate transaminase （AST） from the cultured supernatant of H9c2 cells, which were tested by enzyme-linked immune sorbent assay （ELISA） kits. In addition, it lessened the apoptotic rate as determined by a fluorescein isothiocyanate-annexin V/propidium iodide （FITC-AV/PI） double staining assay. It was also found that HA＋GA might regulate the protein expression associated with the phosphatidylinositol 3-kinase （PI3K）/Akt signaling pathway. Overall, the study demonstrated that HA＋GA protected H9c2 cells against OGD-induced injury, and the signaling mechanism might be related to the PI3K/Akt signaling pathway.