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弓形虫丙二酰化修饰蛋白质组学研究

弓形虫丙二酰化修饰蛋白质组学研究

第九届全国畜牧兽医青年科技工作者学术研讨会

作者：聂兰碧梁勤立杜锐 hany m.Elsheikha 韩乃坚李法财朱兴全

引言/目的弓形虫(Toxoplasmagondii)是一种可以感染包括人类在内的所有温血动物的细胞内寄生虫,世界上约有三分之一的人感染,给免疫系统受损的病人、孕妇及新生儿带来巨大威胁。早期很多研究发现,蛋白质翻译后修饰(PTm)广泛存在于弓形... 详细信息

引言/目的弓形虫(Toxoplasmagondii)是一种可以感染包括人类在内的所有温血动物的细胞内寄生虫,世界上约有三分之一的人感染,给免疫系统受损的病人、孕妇及新生儿带来巨大威胁。早期很多研究发现,蛋白质翻译后修饰(PTm)广泛存在于弓形虫体内,通过调控蛋白质的结构和功能影响弓形虫在宿主细胞上的增殖,移行和致病力。丙二酰-CoA是沟通糖代谢与脂肪酸合成途径的桥梁,丙二酰化修饰是以丙二酰-CoA为修饰供体的一种新型酰基化修饰。

关键词：弓形虫病液相色谱-串联质谱联用技术组学研究

Performance of WSNs under the Effect of Collisions and Interference

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Wireless Sensor Network 2014年第6期6卷 93-103页

作者： mark Onsy Ramy Salah michael makar Ghada Badawi Ahmed Kenawy Hassan H. Halawa Tarek K. Refaat Ramez m. Daoud Hassanein H. Amer hany m. ElSayed magdy El Soudani Electronics and Communication Department Cairo University Giza Egypt Electronics Engineering Department American University in Cairo Cairo Egypt School of Electrical Engineering and Computer Science University of Ottawa Ottawa Canada

This paper proposes a methodology for calculating the energy consumed by a Wireless Sensor Network as well as its throughput under the effect of a jamming node modelling interference to account for collisions and retransmissions. Accordingly, the proposed methodology takes into consideration retransmissions and data dropped due to interference and collisions simultaneously. Simulations are conducted using OPNET to model various scenarios utilizing off-the-shelf wireless communication standards, namely ZigBee, Wi-Fi and Low Power Wi-Fi. A figure of merit is developed to offer more representative results for applications with different requirements. In achieving different requirements for a given application, there is a clear trade-off between energy consumption and throughput.

关键词： WSN Collisions Interference Goodput IEEE 802.11 IEEE 802.15.4 Low Power Wi-Fi ZigBee Energy Consumption

Immunization with plasmid DNA expressing Heat Shock Protein 40 confers prophylactic protection against chronic Toxoplasma gondii infection in Kunming mice

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Immunization with plasmid DNA expressing Heat Shock Protein ...

第十一届全国寄生虫学青年工作者学术研讨会

作者： LI Zhong-Yuan LU Jing ZHANG Nian-Zhang hany m.Elsheikha HOU Jun-Ling GUO Hai-Ting ZHU Xing-Quan College of Animal Science and Technology Anhui Agricultural University State Key Laboratory of Veterinary Etiological Biology Key Laboratory of Veterinary Parasitology of Gansu ProvinceLanzhou Veterinary Research InstituteChinese Academy of Agricultural Sciences Faculty of medicine and Health Sciences School of Veterinary Medicine and ScienceUniversity of NottinghamSutton Bonington Campus College of Biological Science and Technology Heilongjiang Bayi Agricultural University

Objective Toxoplasma gondii causes one of the most common protozoal diseases of humans and animals *** the aim of designing an effective vaccine against *** infection,we have examined the immunogenicity of a DNA vaccine expressing heat shock protein 40(HSP40) against challenge with ***(type I RH and type II Pru) strains in Kunming *** The plasmid p VAX1-HSP40 was constructed and used to immunize mice by intramuscular injection for three sequential immunizations with two-week intervals,and the immune responses including specific antibodies,lymphocyte proliferation,cytokines and T lymphocyte subclasses,the survival times of RH-infected mice and the number of cysts in brains of Pru-infected ones were *** This immunization regimen significantly reduced parasite cyst burden in p VAX1-HSP40-immunized mice(1871.9 ± 142.3) compared with control mouse groups immunized with p VAX1(3479.2 ± 204.4),phosphate buffered saline(3024.4 ± 212.8),or left untreated(3275.0 ± 179.8) as healthy controls(P mmunization failed to protect mice against challenge with the virulent RH *** was significant increase in T lymphocyte subclasses(CD3 e+CD4+ T and CD3 e+CD8 a+ T lymphocytes) in splenic tissues in immunized mice compared with controls(P mphocyte proliferation and concentration of cytokines(IFN-γ,IL-2,IL-4,IL-10 and IL-12 p70) were not significantly different between immunized and control mouse groups(P mmune responses and achieved a significant reduction in the number of brain cysts in Pru-infected mice,and thus can be tested in future immunization studies along with plasmids containing other immunogenic proteins as a cocktail vaccine to fully abolish chronic toxoplasmosis.

关键词： Toxoplasma gondii HSP40 DNA vaccine chronic infection immune evaluation

Novel roles of dense granule protein 12(GRA12) in Toxoplasma gondii infection

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Novel roles of dense granule protein 12(GRA12) in Toxoplasma...

中国畜牧兽医学会兽医寄生虫学分会第一届青年科学家学术论坛

作者： Jin-Lei Wang meng-Jie Bai hany m.Elsheikha Qin-Li Liang Ting-ling Li Xue-Zhen Cao Xing-Quan Zhu State Key Laboratory of Veterinary Etiological Biology Key Laboratory of Veterinary Parasitology of Gansu ProvinceLanzhou Veterinary Research InstituteChinese Academy of Agricultural Sciences Faculty of medicine and Health Sciences School of Veterinary Medicine and ScienceUniversity of NottinghamSutton Bonington Campus College of Veterinary medicine South China Agricultural University

Dense granule protein 12(GRA12) is implicated in a range of processes related to the establishment of Toxoplasma gondii infection,such as the formation of the intravacuolar network(IVN) produced within the parasitophorous vacuole(PV).This protein is thought to be important for ***-host interaction,pathogenesis,and immune evasion,but their exact role remains *** this study,the contributions of GRA12 to the molecular pathogenesis of *** infection were examined in vitro and in *** of GRA12 in type I RH and type II Pru *** strains although did not affect parasite growth and replication in vitro,it caused a significant reduction in the parasite virulence and tissue cyst burden in *** △gra12 mutants were more vulnerable to be eliminated by host immunity,without the accumulation of immunity-related GTPase a6(Igra6) onto the PV *** ultrastructure of intravacuolar network(IVN) in △gra12 mutants appeared normal,suggesting that GRA12 is not required for the IVN *** deletion of GRA12 and ROP18 induced more severe attenuation of virulence compared to single △gra12 or △rop18 mutant strains,suggesting that GRA12 may act synergistically with ROP18 in *** *** these findings indicate that although GRA12 is not essential for parasite growth and replication in vitro,it contributes to the virulence and growth of *** in mice.

关键词： Toxoplasma gondii dense granule protein 12 rhoptry protein 18 intravacuolar network virulence immunity-related GTPases

Gra17和Gra23基因缺失对Ⅱ型弓形虫生长、毒力和免疫原性的影响

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Gra17和Gra23基因缺失对Ⅱ型弓形虫生长、毒力和免疫原性的影响

第九届全国畜牧兽医青年科技工作者学术研讨会

作者：李婷婷王金磊梁勤立孙黎秀张海生张芝玮朱兴全 hany m.Elsheikha

引言/目的刚地弓形虫(Toxoplasmagondii)是一种非常成功的专性细胞内寄生的机会性致病原虫。弓形虫从致密的颗粒细胞器中分泌出许多致密的颗粒蛋白(GRAs)来操纵宿主细胞。这些效应蛋白中的两个蛋白(GRA17和GRA23)参与了在弓形虫液泡和... 详细信息

引言/目的刚地弓形虫(Toxoplasmagondii)是一种非常成功的专性细胞内寄生的机会性致病原虫。弓形虫从致密的颗粒细胞器中分泌出许多致密的颗粒蛋白(GRAs)来操纵宿主细胞。这些效应蛋白中的两个蛋白(GRA17和GRA23)参与了在弓形虫液泡和宿主细胞质之间的分子运输。然而,它们在建立慢性感染中的作用仍然还不清晰。在这项研究中,使用CRISPR-Cas9敲除弓形虫Pru株(Ⅱ型)中的gra17和gra23基因,并在昆明小鼠中探究其突变株的生长,毒力,建立慢性感染的能力以及免疫原性。

关键词：弓形虫 Gra17 Gra23 基因缺失免疫原性

弓形虫tkl1缺失株是一株对小鼠抗急性、慢性和先天性弓形虫病感染有效的疫苗

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弓形虫tkl1缺失株是一株对小鼠抗急性、慢性和先天性弓形虫病感染...

第九届全国畜牧兽医青年科技工作者学术研讨会

作者：王金磊梁勤立李婷婷贺君君白梦捷曹雪珍 hany m.Elsheikha 朱兴全

引言/目的刚地弓形虫(Toxoplasmagondii)简称弓形虫,其中宿主广泛,可以感染几乎所有的温血动物(包括人类),是一种非常成功的专性细胞内寄生的机会性致病原虫。人类主要是通过摄入含有组织包囊的生的或未煮熟的肉、被卵囊污染的食物或水... 详细信息

引言/目的刚地弓形虫(Toxoplasmagondii)简称弓形虫,其中宿主广泛,可以感染几乎所有的温血动物(包括人类),是一种非常成功的专性细胞内寄生的机会性致病原虫。人类主要是通过摄入含有组织包囊的生的或未煮熟的肉、被卵囊污染的食物或水,或先天通过胎盘感染弓形虫病。目前治疗弓形虫病主要依赖于抗寄生虫药物的使用,这些药物的疗效因副作用以及无法消除组织包囊受到限制。缺乏酪氨酸激酶样1 (tkl1)的弓形虫对宿主细胞的附着能力有限,进而损害了体外裂解周期中的寄生虫进程,并导致对小鼠的毒力丧失,表明tk1是具有潜在免疫原性的必需毒力因子。

关键词：弓形虫病免疫接种缺失株

multi-Node Fault-Tolerant Two-Cell Real-Time S2A Network

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Intelligent Control and Automation 2016年第2期7卷 25-38页

作者： merna N. Abou Eita mostafa W. Hussein Ahmed A. Ibrahim Shereen S. Abouelazayem mennatallah A. morsi Eslam A. moustafa Hassan H. Halawa Ramez m. Daoud Hassanein H. Amer hany m. ElSayed Electronics and Communications Engineering Department The American University in Cairo New Cairo Egypt Electronics and Communications Engineering Department Cairo University Giza Egypt

This paper presents a novel fault-tolerant networked control system architecture consisting of two cells working in-line. This architecture is fault-tolerant at the level of the controllers as well as the sensors. Each cell is based on the sensor-to-actuator approach and has an additional supervisor node. It is proven, via analysis as well as OmNeT++ simulations that the production line succeeds in meeting all control system requirements with no dropped or over-delayed packets. A reliability analysis is then undertaken to quantitatively estimate the increase in reliability due to the introduction of fault-tolerance.

关键词： Fault-Tolerance NCS S2A In-Line TmR Ethernet OmNeT++ Reliability

Fasciola gigantica tegumental calcium-binding EF-hand protein 4(Fg-CaBP4) exerts immunomodulatory effects on goat monocytes

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Fasciola gigantica tegumental calcium-binding EF-hand protei...

2021中国畜牧兽医学会兽医寄生虫学分会第16次学术研讨会

作者： muhammad Ehsan Rui-Si Hu Jun-Ling Hou hany m.Elsheikha Xiao-Dong Li Pan-Hong Liang Zhi-Wei Zhang Xing-Quan Zhu

The liver fluke Fasciola gigantica produces a number of proteins during progression of infection inside the *** of these proteins can modulate the host immune ***,we investigated the effect of a recombinant tegumental calcium-binding EF-hand protein 4 from ***(rFg-CaBP4) on the ability of goat monocytes to produce *** isopropyl-β-dthiogalactopyranoside-induced rFg-CaBP4 protein was recognized by rat sera containing polyclonal antibodies raised against rFg-CaBP4,and its binding to the surface of monocytes was confirmed by immunofluorescence *** analysis localized native CaBP4 to the oral sucker,pharynx,genital pore and acetabulum as well as the tegument of adult *** ***-incubation of rFg-CaBP4 with concanavalin A-stimulated monocytes increased the production of interleukin 2(IL-2),IL-4,interferon-y,and transforming growth factor-β and decreased the level of ***,no change in the concentration of tumor necrosis factor-α was ***4 significantly induced the mHC-Ⅱ expression,but reduced the expression of mHC-Ⅰexpression on monocytes,in a dose-dependent *** findings support the role of CaBP4 as a modulator of the host immune response and implicate rFg-CaBP4 as a potential therapeutic target for the development of intervention to prevent or treat fascioliasis.

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一套束流匹配程序的剂量学评价(英文)

The Chinese-German Journal of Clinical Oncology 2014年第2期13卷 89-93页

作者： Ehab m.Attalla H.S.Abou-Elenein hany Ammar Ismail Eldesoky Children's Cancer Hospital National Cancer Institute Cairo University

Objective: The dosimetric characteristics for linear accelerators with the same model, and nominal energy are known to be very similar, as long as the machines are unaltered from the manufacturer's original specifications. In this preliminary study, a quantitative investigation of the similarity in the basic photon and electron dosimetry data from the Siemens Oncor linear accelerators at our hospital(Children's Cancer Hospital, Cairo, Egypt) was reported. methods: The output factor(OF), wedge factors(WF), percentage depth dose(PDD), and beam profile for the 6 and 10 mV photon beams were measured. Results: The measured output factors varied by less than about 1% for each field size. The difference between the maximum and minimum PDD values at each depth was less than about 1%. The difference between the beam flattnes and symetry was no more than 1% at all off-axis distances. For electron the results showed that the PDD, OF, and the beam profiles were matched within 1% differences. Conclusion: These results strongly suggest that it is feasible to establish one reference photon and electron dosimetry data set for the two machines and nominal energies.

关键词：剂量学特性直线加速器评价程序测定数据梁 PDD 光子束