Immunization with plasmid DNA expressing Heat Shock Protein 40 confers prophylactic protection against chronic Toxoplasma gondii infection in Kunming mice
作者单位：College of Animal Science and TechnologyAnhui Agricultural University State Key Laboratory of Veterinary Etiological BiologyKey Laboratory of Veterinary Parasitology of Gansu ProvinceLanzhou Veterinary Research InstituteChinese Academy of Agricultural Sciences Faculty of Medicine and Health SciencesSchool of Veterinary Medicine and ScienceUniversity of NottinghamSutton Bonington Campus College of Biological Science and TechnologyHeilongjiang Bayi Agricultural University
会议名称：《第十一届全国寄生虫学青年工作者学术研讨会》
会议日期：2018年
学科分类：090602[农学-预防兽医学] 09[农学] 0906[农学-兽医学]
基 金：support was provided,in part,by the Open Funds of the State Key Laboratory of Veterinary Etiological Biology(Grant No.SKLVEB2016KFKT015) the General Financial Grants of the Postdoctoral Science Foundation(Grant Nos.2017M612056 and 2017B188) the Elite Program of Chinese Academy of Agricultural Sciences and the Agricultural Science and Technology Innovation Program(ASTIP)(Grant No.CAAS-ASTIP-2016-LVRI-03)
关 键 词：Toxoplasma gondii HSP40 DNA vaccine chronic infection immune evaluation
摘 要：Objective Toxoplasma gondii causes one of the most common protozoal diseases of humans and animals *** the aim of designing an effective vaccine against *** infection,we have examined the immunogenicity of a DNA vaccine expressing heat shock protein 40(HSP40) against challenge with ***(type I RH and type II Pru) strains in Kunming *** The plasmid p VAX1-HSP40 was constructed and used to immunize mice by intramuscular injection for three sequential immunizations with two-week intervals,and the immune responses including specific antibodies,lymphocyte proliferation,cytokines and T lymphocyte subclasses,the survival times of RH-infected mice and the number of cysts in brains of Pru-infected ones were *** This immunization regimen significantly reduced parasite cyst burden in p VAX1-HSP40-immunized mice(1871.9 ± 142.3) compared with control mouse groups immunized with p VAX1(3479.2 ± 204.4),phosphate buffered saline(3024.4 ± 212.8),or left untreated(3275.0 ± 179.8) as healthy controls(P 0.01).However,immunization failed to protect mice against challenge with the virulent RH *** was significant increase in T lymphocyte subclasses(CD3 e+CD4+ T and CD3 e+CD8 a+ T lymphocytes) in splenic tissues in immunized mice compared with controls(P 0.05).However,the level of antibodies,lymphocyte proliferation and concentration of cytokines(IFN-γ,IL-2,IL-4,IL-10 and IL-12 p70) were not significantly different between immunized and control mouse groups(P 0.05).Conclusion These data indicate that p VAX1-HSP40 induced specific immune responses and achieved a significant reduction in the number of brain cysts in Pru-infected mice,and thus can be tested in future immunization studies along with plasmids containing other immunogenic proteins as a cocktail vaccine to fully abolish chronic toxoplasmosis.