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中国兽医科学 2024年第4期54卷 566-567页

作者： Jin-Lei Wang Ting-Ting Li Nian-Zhang Zhang meng Wang Li-Xiu Sun Zhi-Wei Zhang Bao-Quan Fu hany m elsheikha Xing-Quan Zhu

弓形虫病是由弓形虫(Toxoplasma gondii)引起的一种重要的人兽共患寄生虫病,对全球公共卫生体系和畜牧业发展造成巨大威胁。据估计,全球大约有三分之一的人口感染了弓形虫。孕妇在怀孕初期首次感染弓形虫,可能会导致胎儿发育异常、流产... 详细信息

弓形虫病是由弓形虫(Toxoplasma gondii)引起的一种重要的人兽共患寄生虫病,对全球公共卫生体系和畜牧业发展造成巨大威胁。据估计,全球大约有三分之一的人口感染了弓形虫。孕妇在怀孕初期首次感染弓形虫,可能会导致胎儿发育异常、流产、畸形或死胎,每年约有超过五万起流产与此相关。因此,弓形虫是孕期必检病原之一。另外,弓形虫引起的动物流产问题也非常严重,绵羊的流产率介于11%~25%,猪的流产率介于5%~15%,每年由此而造成的经济损失超3亿元。

关键词：人兽共患寄生虫病弓形虫流产率裂殖子畜牧业发展三分之一全球公共卫生体外培养

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Seroprevalence of Toxoplasma gondii infection in arthritis patients in eastern China

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Infectious Diseases of Poverty 2017年第1期6卷 1362-1368页

作者： Ai-Ling Tian Yuan-Lin Gu Na Zhou Wei Cong Guang-Xing Li hany m.elsheikha Xing-Quan Zhu State Key Laboratory of Veterinary Etiological Biology Key Laboratory of Veterinary Parasitology of Gansu ProvinceLanzhou Veterinary Research InstituteChinese Academy of Agricultural SciencesLanzhouGansu Province 730046People’s Republic of China Weihaiwei People’s Hospital WeihaiShandong Province 264200People’s Republic of China Affilliated Hospital of medical College Qingdao UniversityQingdaoShandong Province 266000People’s Republic of China School of Basic medicine Dali UniversityDaliYunnan Province 671000People’s Republic of China Faculty of medicine and Health Sciences School of Veterinary Medicine and ScienceUniversity of NottinghamSutton Bonington CampusLoughborough LE125RDUK

Background:There is accumulating evidence for an increased susceptibility to infection in patients with *** sought to understand the epidemiology of Toxoplasma gondii infection in arthritis patients in eastern China,given the paucity of data on the magnitude of *** infection in these ***:Seroprevalence of *** infection was assessed by enzyme-linked immunosorbent assay using a crude antigen of the parasite in 820 arthritic patients,and an equal number of healthy controls,from Qingdao and Weihai cities,eastern ***,clinical and lifestyle information on the study participants were also ***:The prevalence of *** IgG was significantly higher in arthritic patients(18.8%)compared with 12%in healthy controls(Pm antibodies-comparable with 10 control patients(1.5%vs 1.2%).Demographic factors did not significantly influence these seroprevalence *** highest *** infection seropositivity rate was detected in patients with rheumatoid arthritis(24.8%),followed by reactive arthritis(23.8%),osteoarthritis(19%),infectious arthritis(18.4%)and gouty arthritis(14.8%).Seroprevalence rates of rheumatoid arthritis and reactive arthritis were significantly higher when compared with controls(Pme in arthritic patients(odds ratio[OR],1.68;95%confidence interval[CI]:1.24-2.28;P=0.001).Conclusions:These findings are consistent with and extend previous results,providing further evidence to support a link between contact with cats and an increased risk of *** *** study is also the first to confirm an association between *** infection and arthritis patients in *** for better prevention and control of *** infection in arthritis patients are *** registration:This is an epidem

关键词： Toxoplasma gondii Autoimmunity Arthritis Seroprevalence Risk factors

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Toxocariasis:a silent threat with a progressive public health impact

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Infectious Diseases of Poverty 2018年第1期7卷 570-582页

作者： Jia Chen Quan Liu Guo-Hua Liu Wen-Bin Zheng Sung-Jong Hong Hiromu Sugiyama Xing-Quan Zhu hany m.elsheikha State Key Laboratory of Veterinary Etiological Biology Lanzhou Veterinary Research InstituteChinese Academy of Agricultural Sciences1 XujiapingYanchangbuLanzhouGansu Province 730046People’s Republic of China Ningbo University School of medicine NingboZhejiang ProvincePeople’s Republic of China military Veterinary Institute Academy of Military Medical SciencesKey Laboratory of Jilin Province for Zoonosis Prevention and ControlChangchunJilin ProvincePeople’s Republic of China College of Veterinary medicine Hunan Agricultural UniversityChangshaHunan ProvincePeople’s Republic of China Department of medical Environmental Biology Chung-Ang University College of MedicineSeoulSouth Korea Department of Parasitology National Institute of Infectious DiseasesTokyoJapan Faculty of medicine and Health Sciences School of Veterinary Medicine and ScienceUniversity of NottinghamLoughboroughUK

Background:Toxocariasis is a neglected parasitic zoonosis that afflicts millions of the pediatric and adolescent populations worldwide,especially in impoverished *** disease is caused by infection with the larvae of Toxocara canis and ***,the most ubiquitous intestinal nematode parasite in dogs and cats,*** this article,recent advances in the epidemiology,clinical presentation,diagnosis and pharmacotherapies that have been used in the treatment of toxocariasis are *** text:Over the past two decades,we have come far in our understanding of the biology and epidemiology of ***,lack of laboratory infrastructure in some countries,lack of uniform case definitions and limited surveillance infrastructure are some of the challenges that hindered the estimation of global disease *** encompasses four clinical forms:visceral,ocular,covert and *** or misdiagnosis of any of these disabling conditions can result in severe health consequences and considerable medical care ***,multiple diagnostic modalities are available,which if effectively used together with the administration of appropriate pharmacologic therapies,can minimize any unnecessary patient ***:Although progress has been made in the management of toxocariasis patients,there remains much work to be *** of new technologies and better understanding of the pathogenesis of toxocariasis can identify new diagnostic biomarkers,which may help in increasing diagnostic ***,further clinical research breakthroughs are needed to develop better ways to effectively control and prevent this serious disease.

关键词： Toxocara canis Toxocariasis Zoonosis Larva migrans Epidemiology Diagnosis Control

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metabolomic Profiling of mice Serum and brain during Toxoplasmosis Progression Using Liquid Chromatography-mass Spectrometry

Metabolomic Profiling of Mice Serum and brain during Toxopla...

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中国动物学会寄生虫学专业委员会第十届全国寄生虫学青年工作者学术研讨会

作者： Chunxue Zhou Donghui Zhou hany m.elsheikha Guangxue Liu Xun Suo Xingquan Zhu State Key Laboratory of Veterinary Etiological Biology Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences National Animal Protozoa Laboratory and College of Veterinary medicine China Agricultural University Faculty of medicine and Health Sciences School of Veterinary Medicine and Science University of Nottingham Sutton Bonington Campus

Better understanding of the molecular changes associated with disease is essential for identifying new routes to improved therapeutics and diagnostic tests. The aim of this study was to investigate the dynamic changes in the metabolic profile of mouse serum and brain during Toxoplasma gondii infection. We carried out untargeted metabolomic analysis of sera and brains collected from female BALB/c mice experimentally infected with the T. gondii Pru strain(Genotype II). Serum and brain samples were collected at 7, 14 and 21 day post infection(DPI) from infected and control mice and were subjected to liquid chromatography-quadrupole time-of-flight mass spectrometry(LC-Q-TOF-mS)-based global metabolomics analysis. multivariate statistical analysis identified 79 differentially expressed metabolites in ESI+ mode and 74 in ESI- mode in sera of T. gondii-infected mice compared to the control mice. meanwhile, in the brains, the non-targeted metabolomics analysis detected approximately 2,755 retention time-exact mass pairs, of which more than 60 had significantly differential profiles at different stages of infection. Further principal component analysis(PCA) and partial least squares-discrimination analysis(PLS-DA) identified 19 dysregulated metabolites(5 in ESI+ mode and 14 in ESI- mode) in the sreum related to the metabolism of amino acids and energy metabolism. The potential utility of these metabolites as diagnostic biomarkers was validated through receiver operating characteristic(ROC) curve analysis. These findings provide putative metabolite biomarkers for future study and allow for hypothesis generation about the pathophysiology of toxoplasmosis.

关键词： Toxoplasma gondii metabolomics serum brain LC-mS

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Global iTRAQ-based proteomic profiling of Toxoplasma gondii oocysts during sporulation

Global iTRAQ-based proteomic profiling of Toxoplasma gondii ...

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中国动物学会寄生虫学专业委员会第十届全国寄生虫学青年工作者学术研讨会

作者： Dong Hui Zhou Chun Xue Zhou hany m.elsheikha Shuai He Qian Li Xing Quan Zhu Xun Suo State Key Laboratory of Veterinary Etiological Biology Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences National Animal Protozoa Laboratory and College of Veterinary medicine China Agricultural University Faculty of medicine and Health Sciences School of Veterinary Medicine and Science University of Nottingham Sutton Bonington Campus College of Animal Science and Technology Anhui Agricultural University School of Basic medicine Dali University

Toxoplasma gondii is a medically and economically important protozoan parasite. However, the molecular mechanisms of its sporulation remain largely unknown. Here, we applied i TRAQ coupled with 2D LC–mS/mS proteomic analysis to investigate the proteomic expression profile of T. gondii oocysts during sporulation. Of the 2095 non-redundant proteins identified, 587 were identified as differentially expressed proteins(DEPs). Based on Gene Ontology enrichment and KEGG pathway analyses the majority of these DEPs were found related to the metabolism of amino acids, carbon and energy. Protein interaction network analysis generated by STRING identified ATP-citrate lyase(ACL), GmP synthase, ImP dehydrogenase(ImPDH), poly(ADP-ribose) glycohydrolase(PARG), and bifunctional dihydrofolate reductase-thymidylate synthase(DHFR-TS) as the top five hubs. We also identified 25 parasite virulence factors that were expressed at relatively high levels in sporulated oocysts compared to non-sporulated oocysts, which might contribute to the infectivity of mature oocysts. Considering the importance of oocysts in the dissemination of toxoplasmosis these findings may help in the search of protein targets with a key role in infectiousness and ecological success of oocysts, creating new opportunities for the development of better means for disease prevention. Biological significance: The development of new preventative interventions against T. gondii infection relies on an improved understanding of the proteome and chemical pathways of this parasite. To identify proteins required for the development of environmentally resistant and infective T. gondii oocysts, we compared the proteome of non-sporulated(immature) oocysts with the proteome of sporulated(mature, infective) oocysts. i TRAQ 2D-LC-mS/mS analysis revealed proteomic changes that distinguish non-sporulated from sporulated oocysts. many of the differentially expressed proteins were involved in metabolic pathways and 25 virulence factors we

关键词： Toxoplasma gondii oocyst sporulation iTRAQ proteomics

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Novel roles of dense granule protein 12(GRA12) in Toxoplasma gondii infection

Novel roles of dense granule protein 12(GRA12) in Toxoplasma...

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中国畜牧兽医学会兽医寄生虫学分会第一届青年科学家学术论坛

作者： Jin-Lei Wang meng-Jie Bai hany m.elsheikha Qin-Li Liang Ting-ling Li Xue-Zhen Cao Xing-Quan Zhu State Key Laboratory of Veterinary Etiological Biology Key Laboratory of Veterinary Parasitology of Gansu ProvinceLanzhou Veterinary Research InstituteChinese Academy of Agricultural Sciences Faculty of medicine and Health Sciences School of Veterinary Medicine and ScienceUniversity of NottinghamSutton Bonington Campus College of Veterinary medicine South China Agricultural University

Dense granule protein 12(GRA12) is implicated in a range of processes related to the establishment of Toxoplasma gondii infection,such as the formation of the intravacuolar network(IVN) produced within the parasitophorous vacuole(PV).This protein is thought to be important for ***-host interaction,pathogenesis,and immune evasion,but their exact role remains *** this study,the contributions of GRA12 to the molecular pathogenesis of *** infection were examined in vitro and in *** of GRA12 in type I RH and type II Pru *** strains although did not affect parasite growth and replication in vitro,it caused a significant reduction in the parasite virulence and tissue cyst burden in *** △gra12 mutants were more vulnerable to be eliminated by host immunity,without the accumulation of immunity-related GTPase a6(Igra6) onto the PV *** ultrastructure of intravacuolar network(IVN) in △gra12 mutants appeared normal,suggesting that GRA12 is not required for the IVN *** deletion of GRA12 and ROP18 induced more severe attenuation of virulence compared to single △gra12 or △rop18 mutant strains,suggesting that GRA12 may act synergistically with ROP18 in *** *** these findings indicate that although GRA12 is not essential for parasite growth and replication in vitro,it contributes to the virulence and growth of *** in mice.

关键词： Toxoplasma gondii dense granule protein 12 rhoptry protein 18 intravacuolar network virulence immunity-related GTPases

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Immunization with plasmid DNA expressing Heat Shock Protein 40 confers prophylactic protection against chronic Toxoplasma gondii infection in Kunming mice

Immunization with plasmid DNA expressing Heat Shock Protein ...

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第十一届全国寄生虫学青年工作者学术研讨会

作者： LI Zhong-Yuan LU Jing ZHANG Nian-Zhang hany m.elsheikha HOU Jun-Ling GUO Hai-Ting ZHU Xing-Quan College of Animal Science and Technology Anhui Agricultural University State Key Laboratory of Veterinary Etiological Biology Key Laboratory of Veterinary Parasitology of Gansu ProvinceLanzhou Veterinary Research InstituteChinese Academy of Agricultural Sciences Faculty of medicine and Health Sciences School of Veterinary Medicine and ScienceUniversity of NottinghamSutton Bonington Campus College of Biological Science and Technology Heilongjiang Bayi Agricultural University

Objective Toxoplasma gondii causes one of the most common protozoal diseases of humans and animals *** the aim of designing an effective vaccine against *** infection,we have examined the immunogenicity of a DNA vaccine expressing heat shock protein 40(HSP40) against challenge with ***(type I RH and type II Pru) strains in Kunming *** The plasmid p VAX1-HSP40 was constructed and used to immunize mice by intramuscular injection for three sequential immunizations with two-week intervals,and the immune responses including specific antibodies,lymphocyte proliferation,cytokines and T lymphocyte subclasses,the survival times of RH-infected mice and the number of cysts in brains of Pru-infected ones were *** This immunization regimen significantly reduced parasite cyst burden in p VAX1-HSP40-immunized mice(1871.9 ± 142.3) compared with control mouse groups immunized with p VAX1(3479.2 ± 204.4),phosphate buffered saline(3024.4 ± 212.8),or left untreated(3275.0 ± 179.8) as healthy controls(P mmunization failed to protect mice against challenge with the virulent RH *** was significant increase in T lymphocyte subclasses(CD3 e+CD4+ T and CD3 e+CD8 a+ T lymphocytes) in splenic tissues in immunized mice compared with controls(P mphocyte proliferation and concentration of cytokines(IFN-γ,IL-2,IL-4,IL-10 and IL-12 p70) were not significantly different between immunized and control mouse groups(P mmune responses and achieved a significant reduction in the number of brain cysts in Pru-infected mice,and thus can be tested in future immunization studies along with plasmids containing other immunogenic proteins as a cocktail vaccine to fully abolish chronic toxoplasmosis.

关键词： Toxoplasma gondii HSP40 DNA vaccine chronic infection immune evaluation

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Fasciola gigantica tegumental calcium-binding EF-hand protein 4(Fg-CaBP4) exerts immunomodulatory effects on goat monocytes

Fasciola gigantica tegumental calcium-binding EF-hand protei...

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2021中国畜牧兽医学会兽医寄生虫学分会第16次学术研讨会

作者： muhammad Ehsan Rui-Si Hu Jun-Ling Hou hany m.elsheikha Xiao-Dong Li Pan-Hong Liang Zhi-Wei Zhang Xing-Quan Zhu

The liver fluke Fasciola gigantica produces a number of proteins during progression of infection inside the *** of these proteins can modulate the host immune ***,we investigated the effect of a recombinant tegumental calcium-binding EF-hand protein 4 from ***(rFg-CaBP4) on the ability of goat monocytes to produce *** isopropyl-β-dthiogalactopyranoside-induced rFg-CaBP4 protein was recognized by rat sera containing polyclonal antibodies raised against rFg-CaBP4,and its binding to the surface of monocytes was confirmed by immunofluorescence *** analysis localized native CaBP4 to the oral sucker,pharynx,genital pore and acetabulum as well as the tegument of adult *** ***-incubation of rFg-CaBP4 with concanavalin A-stimulated monocytes increased the production of interleukin 2(IL-2),IL-4,interferon-y,and transforming growth factor-β and decreased the level of ***,no change in the concentration of tumor necrosis factor-α was ***4 significantly induced the mHC-Ⅱ expression,but reduced the expression of mHC-Ⅰexpression on monocytes,in a dose-dependent *** findings support the role of CaBP4 as a modulator of the host immune response and implicate rFg-CaBP4 as a potential therapeutic target for the development of intervention to prevent or treat fascioliasis.

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Toxoplasma gondii tkl1 Deletion mutant Is a Promising Vaccine against Acute,Chronic,and Congenital Toxoplasmosis in mice

Toxoplasma gondii tkl1 Deletion Mutant Is a Promising Vaccin...

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2021中国畜牧兽医学会兽医寄生虫学分会第16次学术研讨会

作者： Jin-Lei Wang Qin-Li Liang Ting-Ting Li Jun-Jun He meng-Jie Bai Xue-Zhen Cao hany m.elsheikha Xing-Quan Zhu

In this study,we generated a tkl1 deletion mutant in the Toxoplasma gondii type 1 RH(RHΔtk1 l) strain and tested the protective efficacies of vaccination using RHΔtkl1 tachyzoites against acute,chronic,and congenital *** infections in Kunming *** vaccinated with RHΔtkl1 mounted a strong humoral and cellular response as shown by elevated levels of ***-specific IgG,IL-2,IL-12,IFN-γ,and *** RHΔtkl1-vaccinated mice survived a lethal challenge with 1×103 tachyzoites of type 1 RH or ToxoDB#9(PYS or TgC7) strain as well as 100 cysts or oocysts of Prugniuad *** mock-vaccinated plus infected mice have *** also protected against cyst-or oocyst-caused chronic infection,reduced vertical transmission caused by oocysts,increased litter size,and maintained body weight of pups brn to dams challenged with10 oocysts on day 5 of *** contrast,all mock-vaccinated plus oocysts-infected dams had aborted,and no fetus has *** dams remained healthy post infection,and their brain cyst burden was significantly reduced compared with mock-vaccinated dams infected with *** vivo depletion of CD4+T cells,CD8+T cells,and B cells revealed that CD8+T cells are involved in the protection of mice against *** ***,adoptive transfer of CD8+T cells from RHΔtkl1-vaccinated mice significantly enhanced the survival of naive mice infected with the pathogenic ***,these data reaffirm the importance of CD8+T cell responses in future vaccine design for toxoplasmosis and present *** tkl1 gene as a promising vaccine candidate.

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Characterization of functions in parasite growth and virulence of four Toxoplasma gondii genes involved in lipid synthesis by CRISPR-Cas9 system

Characterization of functions in parasite growth and virulen...

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2021中国畜牧兽医学会兽医寄生虫学分会第二届青年科学家学术论坛

作者： Jie Liu Ting-Ting Li Qin-Li Liang hany m.elsheikha Dan-Yu Zhao Zhi-Wei Zhang Xiao-Pei Xu meng Wang Xing-Quan Zhu State Key Laboratory of Veterinary Etiological Biology Key Laboratory of Veterinary Parasitology of Gansu ProvinceLanzhou Veterinary Research InstituteChinese Academy of Agricultural Sciences

Fatty acid uptake is extremely important for the survival and growth of the intracellular parasite Toxoplasma *** this study,CRISPR-Cas9 gene editing technology was used to investigate the role of four lipid synthesis enzymes,namely Glycerol-3-phosphate dehydrogenase(G3 PDH),malonyl CoA-acyl carrier protein transacylase(FabD),Acyl-ACP thiolesterase(TE)and diacylglycerol acyltransferase(DGAT),in the virulence and infectivity of Type Ⅰ RH and Type Ⅱ Prugniaud(Pru) *** analysis of the tachyzoites stage showed that FabD protein was located in the apicoplast;however,the expression level of the other three proteins were undetectable in tachyzoites *** with wild type(WT) strains,the growth of RHΔG3 PDH,RHΔTE and RHΔDGAT in vitro and their virulence in vivo were not significantly ***,RHΔFabD exhibited a significantly reduced growth rate,compared with the WT ***,the deletion of FabD attenuated the virulence of Type Ⅱ Pru strain and reduced the formation of cysts in *** data improved our understanding of the role of lipid synthesis enzymes in the pathogenesis of ***.

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