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Effects of a novel bacterial phytase expressed in Aspergillus Oryzae on digestibility of calcium and phosphorus in diets fed to weanling or growing pigs

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journal of Animal Science and Biotechnology 2013年第2期4卷 102-111页

作者： Ferdinando nielsen Almeida Rommel Casilda Sulabo hans Henrik Stein Department of Animal Sciences University of Illinois

In 2 experiments, 48 weanling （initial BW： 13.5 ± 2.4 kg, Exp. 1） were used to determine effects of a novel bacterial 6-phytase and 24 growing pigs （initial BW： 36.2 ± 4.0 kg, Exp. 2） expressed in Aspergillus oryzae on the apparent total tract digestibility （ATTD） of phosphorus and calcium in corn-soybean meal diets fed to weanling and growing pigs. In Exp. 1 and 2, pigs were randomly allotted to 6 dietary treatments using a randomized complete block design and a balanced 2 period changeover design, respectively. In both experiments, 6 diets were formulated. The positive control diet was a corn-soybean meal diet with added inorganic phosphorus （Exp. 1：0.42 and 0.86% standardized total tract digestible phosphorus and total calcium, respectively; Exp. 2：0.32 and 0.79% standardized total tract digestible phosphorus and total calcium, respectively）. A negative control diet and 4 diets with the novel phytase （Ronozyme HiPhos, DSM Nutritional Products Inc., Parsippany, N j） added to the negative control diet at levels of 500, 1,000, 2,000, and 4,000 phytase units （FYT）/kg were also formulated. In Exp. 1, the ATTD of phosphorus was greater （P 〈 0.01） for the positive control diet （60.5%） than for the negative control diet （40.5%）, but increased （linear and quadratic, P 〈 0.01） as phytase was added to the negative control diet （40.5% vs. 61.6%, 65.1%, 68.7%, and 68.0%）. The breakpoint for the ATTD of phosphorus （68.4%） was reached at a phytase inclusion level of 1,016 FYT/kg. In Exp. 2, the ATTD of phosphorus was greater （P〈 0.01） for the positive control diet （59.4%） than for the negative control diet （39.8%） and increased （linear and quadratic, P〈 0.01） as phytase was added to the negative control diet （39.8% vs. 58.1%, 65.4%, 69.1%, and 72.8%）. The breakpoint for the ATTD of phosphorus （69.1%） was reached at a phytase inclusion level of 801 FYT/kg. in conclusion, the novel bacterial 6-phytase improved the ATTD of phosphorus and calcium in both weanling and growing pigs. The optimum level of inclusion for this phytase

关键词： Calcium Digestibility Phosphorus Phytase Pigs

Amino acid digestibility of heat damaged distillers dried grains with solubles fed to pigs

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journal of Animal Science and Biotechnology 2014年第1期5卷 88-97页

作者： Ferdinando nielsen Almeida john Kyaw Htoo john Thomson hans Henrik Stein Department of Animal Sciences University of Illinois1207 W Gregory DrUrbana IL 61801 USA Evonik Industries AG Rodenbacher Chaussee 4Hanau 63457 Germany Evonik Degussa Corporation 1701 Barrett LakesBlvd NW Kennesaw GA 30144 USA

The primary objective of this experiment was to determine the effects of heat treatment on the standardized ileal digestibility （SID） of amino acids （AA） in corn distillers dried grains with solubles （DDGS） fed to growing pigs. The second objective was to develop regression equations that may be used to predict the concentration of SID AA in corn DDGS. A source of corn DDGS was divided into 4 batches that were either not autoclaved or autoclaved at 130℃ for 10, 20, or 30 min. Four diets containing DDGS from each of the 4 batches were formulated with DDGS being the only source of AA and CP in the diets. A N-free diet also was formulated and used to determine the basal endogenous losses of CP and AA. Ten growing pigs （initial BW： 53.5 ＋ 3.9 kg） were surgically equipped with a T-cannula in the distal ileum and allotted to a replicated 5 x4 Youden square design with 5 diets and 4 periods in each square. The SID of CP decreased linearly （P〈 0.05） from 77.9% in non-autoclaved DDGS to 72.1, 66.1, and 68.5% in the DDGS samples that were autoclaved for 10, 20, or 30 min, respectively. The SID of lysine was quadratically reduced （P〈 0.05） from 66.8% in the non-autoclaved DDGS to 54.9, 55.3, and 51.9% in the DDGS autoclaved for 10, 20, or 30 min, respectively. The concentrations of SID Arginine, Histidine, Leucine, Lysine, Methionine, Phenylalanine, or Threonine may be best predicted by equations that include the concentration of acid detergent insoluble N in the model （r2 = 0.76, 0.68, 0.67, 0.84, 0.76, 0.73, or 0.54, respectively）. The concentrations of SID Isoleucine and Valine were predicted （r2 = 0.58 and 0.54, respectively） by the Lysine：CP ratio, whereas the concentration of SID Tryptophan was predicted （r2 = 0.70） by the analyzed concentration of Tryptophan in DDGS. In conclusion, the SID of AA is decreased as a result of heat damage and the concentration of SID AA in heat-damaged DDGS may be predicted by regression equations developed in this experiment.

关键词： Amino acids Digestibility Distillers dried grains with solubles Heat damage

Amino acid digestibility in low-fat distillers dried grains with solubles fed to growing pigs

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journal of Animal Science and Biotechnology 2014年第4期5卷 414-420页

作者： Shelby Marie Curry Diego Mario David Labadan Navarro Ferdinando nielsen Almeida juliana Abranches Soares Almeida hans Henrik Stein Department of Animal Sciences University of Illinois

The objective of this experiment was to determine the standardized ileal digestibility（SID） of amino acids（AA） in 3sources of distillers dried grains with solubles（DDGS） with different concentrations of fat.Twelve growing barrows（initial body weight：76.1 ± 6.2 kg） were randomly allotted to a replicated 6×4 Youden square design with 6 diets and 4 periods.The fat content of the 3 sources of DDGS were 11.5,7.5,and 6.9%respectively.Diets contained 60%DDGS and fat concentration of the diets were 7.5,52,and 5.2%,respectively.Two additional diets containing the 2sources of DDGS with 75 and 6.9%fat were also formulated,and corn oil was added to these diets to increase the concentration of fat in the diets to levels that were calculated to be similar to the diet containing conventional DDGS with 11.5%fat.A N-free diet was also formulated to calculate endogenous losses of crude protein（CP） and AA from the pigs.Pigs were fed experimental diets during four 7-d periods.The first 5 d of each period were an adaptation period and ileal digesta were collected on d 6 and 7 of each period.The apparent ileal digestibililty（AID） and SID of CP and all indispensable AA,except AID Pro and SID of Trp,were greater（P 〈 0.01） in conventional DDGS than in the 2 sources of DDGS with reduced fat.Adding oil to the diets containing the 2 sources of DDGS with reduced fat did not consistently increase SID of AA.In conclusion,conventional DDGS has greater SID values for most AA compared with DDGS that contains less fat and inclusion of additional oil to diets containing low-fat DDGS does not increase AID or SID of AA.The lower AA digestibility in low-fat DDGS could not be overcome by the inclusion of additional fat to the diets.

关键词： Amino acid digestibility Distillers dried grains with solubles Pigs

Front line defenders of the ecological niche!Screening the structural diversity of peptaibiotics from saprotrophic and fungicolous Trichoderma/Hypocrea species

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Fungal Diversity 2014年第6期69卷 117-146页

作者： Christian R.Rohrich Walter M.jaklitsch Hermann Voglmayr Anita Iversen Andreas Vilcinskas Kristian Fog nielsen Ulf Thrane hans von Dohren hans Bruckner Thomas Degenkolb Bioresources Project Group Fraunhofer Institute for Molecular Biology and Applied Ecology(IME)Winchesterstrasse 235394 GiessenGermany Department of Systematic and Evolutionary Botany Faculty Centre of BiodiversityUniversity of ViennaRennweg 141030 ViennaAustria Department of Systems Biology Technical University of DenmarkSoltofts PladsBuilding 2212800 Kgs.LyngbyDenmark Interdisciplinary Research Centre for BioSystems Land Use and Nutrition(IFZ)Department of Applied EntomologyInstitute of Phytopathology and Applied Zoology(IPAZ)University of GiessenHeinrich-Buff-Ring 26-3235392 GiessenGermany Biochemistry and Molecular Biology OE 2 Institute of ChemistryTechnical University of BerlinFranklinstrasse 2910587 BerlinGermany Interdisciplinary Research Centre for BioSystems Land Use and Nutrition(IFZ)Department of Food SciencesInstitute ofNutritional ScienceUniversity of GiessenHeinrich-Buff-Ring 26-3235392 GiessenGermany AB SCIEXGermany GmbH Landwehrstrasse 5464293 DarmstadtGermany Danish Emergency Management Agency Universitetsparken 22100 CopenhagenDenmark

Approximately 950 individual sequences of nonribosomally biosynthesised peptides are produced by the genus Trichoderma/Hypocreathat belong to a perpetually growing class of mostly linear antibiotic oligopeptides,which are rich in the non-proteinogenic α-aminoisobutyric acid(Aib).Thus,they are comprehensively named peptaibiotics.Notably,peptaibiotics represent ca.80% of the total inventory of secondarymetabolites currently known from Trichoderma/Hypocrea.Their unique membrane-modifying bioactivity results from amphipathicity and helicity,thus making them ideal candidates in assisting both colonisation and defence of the natural habitats by their fungal producers.Despite this,reports on the in vivo-detection of peptaibiotics have scarcely been published in the past.In order to evaluate the significance of peptaibiotic production for a broader range of potential producers,we screened nine specimens belonging to seven hitherto uninvestigated fungicolous or saprotrophic Trichoderma/Hypocrea species by liquid chromatography coupled to electrospray high resolution mass spectrometry.Sequences of peptaibiotics found were independently confirmed by analysing the peptaibiome of pure agar cultures obtained by single-ascospore isolation from the specimens.Of the nine species examined,five were screened positive for peptaibiotics.A total of 78 peptaibiotics were sequenced,56(=72%)of which are new.Notably,dihydroxyphenylalaninol and O-prenylated tyrosinol,two C-terminal residues,which have not been reported for peptaibiotics before,were found as well as new and recurrent sequences carrying the recently described tyrosinol residue at their C-terminus.The majority of peptaibiotics sequenced are 18-or 19-residue peptaibols.Structural homologies with‘classical representatives’of subfamily 1(SF1)-peptaibiotics argue for the formation of transmembrane ion channels,which are prone to facilitate the producer capture and defence of its substratum.

关键词： HPLC/QTOF-ESI-HRMS Metabolite profiling Peptaibiotics Peptaibols Aib peptides Trichoderma Hypocrea

A Lesion-Mimic Syntaxin Double Mutant in Arabidopsis Reveals Novel Complexity of Pathogen Defense Signaling

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Molecular Plant 2008年第3期1卷 510-527页

作者： Ziguo Zhang Andrea Lenk Mats X. Andersson Torben Gjetting Carsten Pedersen Mads E. nielsen Mari-Anne Newman Bi-Huei Hou Shauna C. Somerville hans Thordal-Christensen Plant and Soil Science Dept of Agricultural Sciences Faculty of Life Sciences University of Copenhagen Thorvaldsensvej 40 DK-1871 Frederiksberg CDenmark Dept of Plant Bioloqy Carneqie Institution Stanford CA 94305 USA Plant Pathology Dept of Plant Biology Faculty of Life Sciences University of Copenhagen Thorvaldsensvej 40 DK-1871 Frederiksberg C Denmark Research Laboratory Carlsberg Research Center GL Carlsbergvej 10 DK-2500 Valby Copenhagen Denmark

The lesion-mimic Arabidopsis mutant, syp121 syp122, constitutively expresses the salicylic acid （SA） signaling pathway and has low penetration resistance to powdery mildew fungi. Genetic analyses of the lesion-mimic phenotype have expanded our understanding of programmed cell death （PCD） in plants. Inactivation of SA signaling genes in syp121 syp122 only partially rescues the lesion-mimic phenotype, indicating that additional defenses contribute to the PCD. Whole genome transcriptome analysis confirmed that SA-induced transcripts, as well as numerous other known pathogenresponse transcripts, are up-regulated after inactivation of the syntaxin genes. A suppressor mutant analysis of syp121 syp122 revealed that FMO1, ALD1, and PAD4 are important for lesion development Mutant alleles of EDS1, NDR1, RAR1, and SGTlb also partially rescued the lesion-mimic phenotype, suggesting that mutating syntaxin genes stimulates TIR-NB-LRR and CC-NB-LRR-type resistances. The syntaxin double knockout potentiated a powdery mildew- induced HR-like response. This required functional PAD4 but not functional SA signaling. However, SA signaling potentiated the PAD4-dependent HR-like response. Analyses of quadruple mutants suggest that EDS5 and SID2 confer separate SA-independent signaling functions, and that FMO1 and ALD1 mediate SA-independent signals that are NPRl-dependent. These studies highlight the contribution of multiple pathways to defense and point to the complexity of their interactions.

关键词： SYSTEMIC ACQUIRED-RESISTANCE HYPERSENSITIVE CELL-DEATH FLAVIN-CONTAINING MONOOXYGENASE SALICYLIC-ACID DISEASE RESISTANCE

Experimental human pain models in gastro-esophageal reflux disease and unexplained chest pain

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World journal of Gastroenterology 2006年第18期12卷 2805-2817页

作者： Asbjφrn Mohr Drewes Lars Arendt-nielsen Peter Funch-jensen hans Gregersen Center for Visceral Biomechanics and Pain Department of GastroenterologyAalborg Hospitaland Center for Sensory-Motor InteractionsDepartment of Health Science and TechnologyAalborg UniversityDenmark Department of Surgical Gastroenterology L Aarhus HospitalDenmark

Methods related to experimental human pain research aim at activating different nociceptors, evoke pain from different organs and activate specific pathways and mechanisms. The different possibilities for using mechanical, electrical, thermal and chemical methods in visceral pain research are discussed with emphasis of combinations (e.g., the multimodal approach). The methods have been used widely in assessment of pain mechanisms in the esophagus and have contributed to our understanding of the symptoms reported in these patients. Hence abnormal activation and plastic changes of central pain pathways seem to play a major role in the symptoms in some patients with gastro-esophageal reflux disease and in patients with functional chest pain of esophageal origin. These findings may lead to an alternative approach for treatment in patients that does not respond to conventional medical or surgical therapy.

关键词：刺激感觉胃肠道生理学疼痛食管反流疾病

高大冷杉温室Strophosoma象甲的微生物防治(英文)

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安徽农业大学学报 2007年第2期34卷 185-194页

作者：夏洛蒂.尼尔森 Susanne Vestergaard Susanne Harding jФrgen Eilenberg Department of Ecology Copenhagen UniversityFaculty of Life SciencesThorvaldsensvej 401871 Frederiksberg CDenmark

In Denmark,the weevils Strophosoma melanogrammum and S.capitatum cause economic damage in Noble fir due to the adult stage feeding on the needles.No chemical treatments of these weevils are allowed in Denmark,so biological control is an attractive solution.We evaluated the potential for microbial control of larvae of Strophosoma spp.based on laboratory bioassays and field applications,taking effect on both target and non-target into consideration,as well as persistence of the applied fungus.In the laboratory Beauveria bassiana,Paecilomyces farinosus and Metarhizium anisopliae were able to infect and cause mycosis in Strophosoma larvae.Among the tested isolates the most virulent isolate was M.anisopliae BIPESCO 5,which resulted in 80 % mortality.In the field experiment M.anisopliae,isolate BIPESCO 5,was applied to the soil as a conidial suspension against larvae of Strophosoma spp.The effect of the fungus on the target population was monitored at a weekly basis by counts of emerging adult weevils during their activity periods.The population of Strophosoma spp.was reduced by up to 60% in treated plots compared to control plots.The non-target effects of M.anisopliae were studied by sampling insects and ticks from both treated and control plots.Seven days after treatment,two sampled insect orders(Hemiptera and Coleoptera)and ticks were found with prevalences of M.anisopliae above 50%,compared to no infection in the insects collected from control plots.Infections in coccinellids were found as long as 277 days after treatment.However,the effect on population level of non-target is still unexplored.The persistence of the fungus was documented by plating a soil suspension onto agar.We documented that conidia of M.anisopliae could persist in the greenery plantation for at least 418 days after application.

关键词：高大冷杉温室微生物 Strophosoma象甲防治措施

Ultrasonographic study of mechanosensory properties in human esophagus during mechanical distension

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World journal of Gastroenterology 2006年第28期12卷 4517-4523页

作者： Ejnar Larsen Hariprasad Reddy AsbjΦrn Mohr Drewes Lars Arendt-nielsen hans Gregersen Department of Radiology Aalborg HospitalAalborg DK-9100Denmark Center for Visceral Biomechanics and Pain Aalborg HospitalAalborg DK-9100Denmark Laboratory for Experimental Pain Research Center for Sensory-Motor InteractionDepartment of Health TechnologyAalborg UniversityAalborg Dk-9100Denmark

瞄准：在远侧的食管在控制卷的斜面扩张期间用内视镜的 ultrasonography 学习食道的几何学和 mechanosensation。方法：12 个健康志愿者经历了一只袋子的扩张。在直到中等疼痛的扩张期间，感觉紧张在视觉类似物规模(管) 上被估计。食道... 详细信息

瞄准：在远侧的食管在控制卷的斜面扩张期间用内视镜的 ultrasonography 学习食道的几何学和 mechanosensation。方法：12 个健康志愿者经历了一只袋子的扩张。在直到中等疼痛的扩张期间，感觉紧张在视觉类似物规模(管) 上被估计。食道的变丑在不同体积和管层次以多维的段比率和紧张被计算。扩张被做在前并且在反副交感神经生理的药丁基莨菪胺的管理期间。结果：刺激反应(体积 -- 管) 没有或与丁基的管理，曲线没不同莨菪胺。段比率的分析在纵的方向在环绕的方向，在光线的方向的压缩和小张力的段表明了张力的段。一个紧张坡度在粘膜表面与最大的环绕的变丑在整个食道的墙存在。感觉紧张作为紧张的功能指数地增加了。结论：方法提供相关到感觉紧张的食道的变丑坡度的信息。因此，在人的食管学习 mechanosensation 能被用来。进一步的研究被需要为食道的 mechanoreceptors 决定准确变丑刺激。

关键词： Deformation analysis Esophagus Experimental pain Ultrasonography

Antibody administration in experimental influenza increases survival and enhances the effect of oseltamivir

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Health 2012年第10期4卷 933-940页

作者： Brit N.j.Pourroy hans jorn Kolmos Lars P.nielsen Department of Virology Statens Serum InstituteCopenhagenDenmark Research Unite of Clinical Microbiology Institute of Clinical ResearchUniversity of Southern DenmarkOdenseDenmark

Anti-viral chemotherapy plays an important part in treating and preventing influenza illness. However, its effectiveness in severe infections can be debated and a reoccurring problem is the emergence of resistant virus. Passive immunisation has for a long time been and is still used for prophylaxis and treatment of a number of infectious diseases. In this experimental study anti-influenza antibodies were passively administrated to mice, subsequently they were infected with influenza virus and treated with oseltamivir. The aim was to investigate, if anti-influenza antibodies influenced the out come of oseltamivir treatment and development of resistance towards oseltamivir. We show, that oseltamivir alone was not able to effectively prevent a fatal outcome, but that oseltamivir administered together with a limited amount of antibodies, resulted in improvement of the clinical condition of the mice. The results also showed that a higher dosage of antibodies alone were able to protect the mice from a lethal dose of virus. These findings suggest that the effectiveness of oseltamivir depends on the host’s immune response to the influenza virus, and that that passive immunization is an option that should be considered in the in control of influenza.

关键词： Experimental Influenza Passively Administrated Antibodies Convalescent Plasma Passive Immunisation Oseltamivir Lethal Infection Polyclonal IgG Pandemic Control