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Growth of G422 glioma implanted in the mouse brain was affected by the immune ability of the host

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Chinese Medical Journal 2011年第13期124卷 1994-1998页

作者： CHENG Ying-xin LI Fei LU Jia-you LI Mei du Peng XU Gui-lian feng hua Department of Neurosurgery Southwest Hospital the Third Military Medical University Chongqing400038 China Department of Immunology Southwest Hospital the Third Military Medical University Chongqing400038 China

Background It is generally accepted that gliomas are the most common primary brain tumors with poor prognosis. We aimed to explore the relationship of the immunity of the central nervous system and the genesis and development of glioma. Methods G422 glioma was implanted in the brain of BALB/c mice （immuno-competent mice）, nude mice （T cell related immuno-deficient） and complement C3 knock-out mice （complement C3 related immunodeficient）. The survival time of the host, growth and histopathology of the tumor, and concentrations of tumor necrosis factor-α （TNF-α） and interferon-γ （INF-γ） in tumor tissues were assessed. Results Tumor spheres were formed in all mice after injection, and glial fibrillary acidic protein （GFAP） positive staining of the cells declared their glioma origin. The longest median survival time of （44.3±6.0） days was found in BALB/c mice, followed by （24.8±5.2） days in nude mice and the shortest （18.6±5.8） days in complement C3 knock-out mice. Accordingly, the growth of the tumor was fastest in complement C3 knock-out mice, followed by the nude mice and slowest in the BALB/c mice. Although the proportions of infiltrating CD68^± lymphocytes in tumor tissues showed no significant difference （P 〉0.05）, TNF-α level in the nude and C3 knock-out mice, （28.11±4.86） μmol/L and （22.87±6.36） μmol/L respectively, were significantly lower （P 〈0.01） than that in the BALB/c mice, which was （230.21±39.17） μmol/L. The INF-γ level was highest in the BALB/c mice （（180.76±29.19） μmol/L）, followed by the nude mice （（113.46±23.76） μmol/L） and then the C3 knock-out mice （（16.84±4.45） μmol/L）. Conclusions The G422 glioma implanted in the brains of mice with different immune ability would be a useful model for studying the relationship of the immune system and tumor in the central nervous system. Furthermore, the T cells and complement C3 compartments of the immune response may affect the growth of implanted tumors and inflammatory factors such as TNF-α and INF-γ.

关键词： glioma immuno-deficient mice complement C3 brain tumor

Hyperbaric Oxygen Preconditioning Protects Against Traumatic Brain Injury at High Altitude

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Hyperbaric Oxygen Preconditioning Protects Against Traumatic...

首届国际神经修复学会年会

作者： huaNG Shengli feng hua Department of Neurosury of southwest hospital

＜正＞Backgroud:Recent studies have demonstrated that preconditioning with hyperbaric oxygen （HBO） can reduce ischemic and hemorrhagic brain injury. Objectives:The aim of this study was to investigate the effects of HBO preconditioning on traumatic brain injury （TBI） at high altitude and examine the role of matrix metalloproteinase-9

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20-Hydroxyecdysone Improves Neuronal Differentiation of Adult Hippocampal Neural Stem Cells in High Power Microwave Radiation-Exposed Rats

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Biomedical and Environmental Sciences 2022年第6期35卷 504-517页

作者： LIU Jing Jing ZHANG Hong Yan CHEN Xin ZHANG Guang Bin LIN Jiang Kai feng hua CHU Wei hua Department of Neurosurgery and Key Laboratory of Neurotrauma Southwest HospitalThird Military Medical University(Army Medical University)Chongqing 400038China Department of Occupational Health Faculty of Preventive MedicineThird Military Medical University(Army Medical University)Chongqing 400038China

Objective The hippocampus is thought to be a vulnerable target of microwave exposure.The aim of the present study was to investigate whether 20-hydroxyecdysone(20E)acted as a fate regulator of adult rat hippocampal neural stem cells(NSCs).Furthermore,we investigated if 20E attenuated high power microwave(HMP)radiation-induced learning and memory deficits.Methods Sixty male Sprague-Dawley rats were randomly divided into three groups:normal controls,radiation treated,and radiation+20E treated.Rats in the radiation and radiation+20E treatment groups were exposed to HPM radiation from a microwave emission system.The learning and memory abilities of the rats were assessed using the Morris water maze test.Primary adult rat hippocampal NSCs were isolated in vitro and cultured to evaluate their proliferation and differentiation.In addition,hematoxylin&eosin staining,western blotting,and immunofluorescence were used to detect changes in the rat brain and the proliferation and differentiation of the adult rat hippocampal NSCs after HPM radiation exposure.Results The results showed that 20E induced neuronal differentiation of adult hippocampal NSCs from HPM radiation-exposed rats via the Wnt3a/β-catenin signaling pathway in vitro.Furthermore,20E facilitated neurogenesis in the subgranular zone of the rat brain following HPM radiation exposure.Administration of 20E attenuated learning and memory deficits in HPM radiation-exposed rats and frizzled-related protein(FRZB)reduced the 20E-induced nuclear translocation ofβ-catenin,while FRZB treatment also reversed 20E-induced neuronal differentiation of NSCs in vitro.Conclusion These results suggested that 20E was a fate regulator of adult rat hippocampal NSCs,where it played a role in attenuating HPM radiation-induced learning and memory deficits.

关键词： High power microwave Hippocampus 20-hydroxyecdysone Learning and memory

Effects of preconditioning with hyperbaric oxygen on expression of GFAP and Nestin after spinal cord injury in rats

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Effects of preconditioning with hyperbaric oxygen on express...

第十六届国际高气压医学学术会议暨第十七届全国高压氧医学...

作者： Peigang Lu hua feng Yongzhi Xia Weihua Chu Guoqi Gong Department of Neurosurgery southwest hospital Third Military Medical University

＜正＞Objective:To study the effects of preconditioning with hyperbaric oxygen on expression of GFAP and Nestin after spinal cord injury in rats at different time.Methods:fifty-five adult Sprague-Dawley adult rats, weighing from 250 to 300g,were randomly divided into 3 groups,hyperbaric oxygen preconditional group（25,n=5）,normal injury group（25,n=5）and control group（5,n=5）.In the experimental groups,the rat

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Glibenclamide pretreatment attenuates early hematoma expansion of warfarin-associated intracerebral hemorrhage in rats by alleviating perihematomal blood-brain barrier dysfunction

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Chinese Neurosurgical Journal 2024年第1期10卷 8-19页

作者： Zongwei Zeng Liang Liang Zhou feng Peiwen Guo Xiaoke Hao Jishu Xian hua feng Yujie Chen Zhi Chen Department of Neurosurgery and State Key Laboratory of Trauma Burn and Combined InjurySouthwest HospitalThird Military Medical University(Army Medical University)Chongqing 400038China Department of Neurosurgery The 945Th Hospital of PLAYaanSichuan Province 625099China Department of Rehabilitation Southwest HospitalThird Military Medical University(Army Medical University)Chongqing 400038China Chongqing Key Laboratory of Precision Neuromedicine and Neuroregeneration Southwest HospitalThird Military Medical University(Army Medical University)Chongqing 400038China Chongqing Clinical Research Center for Neurosurgery Southwest HospitalThird Military Medical University(Army Medical University)Chongqing 400038China

Background Hematoma expansion is a determinant of poor outcome of intracerebral hemorrhage but occurs frequently,especially in warfarin-associated intracerebral hemorrhage(W-ICH).In the present study,we employ the warfarin-associated intracerebral hemorrhage(W-ICH)rat model,to explore the efficacy and potential mechanism of glibenclamide pretreatment on hematoma expansion after intracerebral hemorrhage,hoping to provide proof of concept that glibenclamide in stroke primary and secondary prevention is also potentially beneficial for intracerebral hemorrhage patients at early stage.Methods In the present study,we tested whether glibenclamide,a common hypoglycemic drug,could attenuate hematoma expansion in a rat model of W-ICH.Hematoma expansion was evaluated using magnetic resonance imaging;brain injury was evaluated by brain edema and neuronal death;and functional outcome was evaluated by neurological scores.Then blood-brain barrier integrity was assessed using Evans blue extravasation and tight junction-related protein.Results The data indicated that glibenclamide pretreatment significantly attenuated hematoma expansion at 24 h after W-ICH,thus mitigating brain edema and neuronal death and promoting neurological function recovery,which may benefit from alleviating blood-brain barrier disruption by suppressing matrix metallopeptidase-9.Conclusions The results indicate that glibenclamide pretreatment in stroke primary and secondary prevention might be a promising therapy for hematoma expansion at the early stage of W-ICH.

关键词： Intracerebral hemorrhage Warfarin Glibenclamide Hematoma expansion Blood-brain barrier Matrix metallopeptidase-9

Effects of preconditioning with hyperbaric oxygen on neural cell apoptosis after spinal cord injury in rats

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Effects of preconditioning with hyperbaric oxygen on neural ...

第十六届国际高气压医学学术会议暨第十七届全国高压氧医学...

作者： Peigang Lu hua feng Yongzhi Xia Guoqi Gong Mei Li Department of Neurosurgery southwest hospital Third Military Medical University Chengdu China

＜正＞Objective:To study the effects of preconditioning with hyperbaric oxygen on neural cell apoptosis and expression of Caspase-3 after spinal cord injury in rats at different time.Methods:fifty-five adult Sprague-Dawley adult rats,weighing from 250 to 300g,were randomly divided into 3 groups,hyperbaric oxygen preconditional group（25,n=5）,normal injury group（25,n=5）and control group（5,n=5）.In the

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The effect of ecdysterone on cerebral vasospasm following experimental subarachnoid hemorrhage in vitro and in vivo

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The effect of ecdysterone on cerebral vasospasm following ex...

中国医师协会神经外科医师分会第二届全国代表大会

作者： TANG Wei-hua LIU Zhi ZHANG John H XI Guo-hua CHEN Zhi feng hua Department of Neurosurgery,southwest hospital,Third Millitary Medical University,Chongqing. 重庆西南医院神经外科

＜正＞Objectives cerebral vasospasm was the dread complication of a raptured intracranial aneurysm.Worldwide effort has led to many promising experimental treatments but none were confirmed to be effective in clinical trials.Ecdysterone is a kind of insect steroid hormone.Our previous study showed that ecdysterone might prevent CVS in vitro.Even after all these studies,rare attempts have been made to test the effect of Eedysterone on VAFs,a processes known to play an important role in various pathogenic vascular conditions. Thus,we tested the hypothesis that Eedysterone could affect VAF’s characteristic and do effect in SAIl induced cerebral vasospasm.Methods 100μM OxyHb were used in the in vitro study to mimic the clinical situation,the effects of OxyHb on cells proliferation and migratio of cultured aortic smooth muscle cells was investigated.In the in vivo study,20 rabbits were equally divided into 4 groups:control group,SAH group,SAH/Nimodipine group and SAH/ecdysterone group.Changes of neurological function and cerebral angiogram of rabbits were observed after SAH.Results OxyHb increased the proliferation of VAFs at 24h.Ecdysterone co-treatment was something like to suppress proliferation.Cell cycle analysis indicated that EDS inhibited vascular advential fibroblasts from G1 to S progression.Results of migration assay showed that 100 μM OxyHb obviously prompt vascular advential fibroblasts migration and EDS would attenut this effect.In SAH/Nimodipine and SAH/ecdysterone groups,neural deficit, cerebral vasospasm and structural changes in basilar artery were alleviated with Nimodipine or ecdysterone treatment.Conclusions Ecdysterone could affect VAF’s characteristic and attenuate vasospasm after SAH.

关键词： Cerebral vasospasm Ecdysterone Oxyhemoglobin Rabbit animal model Vascular advential fibroblasts

Risk factors for early progression of diffuse low-grade glioma in adults

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Chinese Neurosurgical Journal 2023年第1期9卷 9-17页

作者： Long Wang Xuegang Li Tunan Chen Chao Zhang Jiantao Shi hua feng Fei Li Department of Neurosurgery Southwest HospitalThird Military Medical University(Army Medical University)Chongqing 400038China

Background:To explore the risk factors for early progression of diffuse low-grade glioma in adults.Methods:A retrospective analysis of pathologic and clinical data of patients diagnosed with diffuse low-grade gliomas at southwest hospital between January 2010 and December 2014.The progression-free survival(PFS)less than 60 months was classified as the early progress group,and the PFS greater than 60 months was the control group for comparative analysis.Results:A total of 138 patients were included in this study,including 94 cases of astrocytoma and 44 cases of oligodendroglioma.There were 63 cases with 100%resection,56 cases with 90-100%resection degree,and 19 cases with resection degree60 months.The two groups were compared for statistical analysis.In univariate analysis,there were significant differences in tumor subtype(p=0.005),range(p=0.011),volume(p=0.005),location(p=0.000),and extent of resection(p=0.000).Multifactor analysis shows tumor location(HR=4.549,95%CI:1.324-15.634,p=0.016)and tumor subtype(HR=3.347,95%CI=1.373-8.157,p=0.008),and imcomplete resection is factors influencing early progression of low-grade glioma.Conclusions:Low-grade gliomas involving deep location such as basal ganglia,inner capsule,and corpus callosum are more likely to progress early,while incomplete resection is a risk factor in early progression of astrocytoma.

关键词： Risk factors Early progression Low-grade glioma

A three-dimensional matrix system containing melatonin and neural stem cells repairs damage from traumatic brain injury in rats

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Neural Regeneration Research 2022年第11期17卷 2512-2517页

作者： Xuan-Yu Fang Da-Wei Zhao Chao Zhang Hong-Fei Ge Xu-Yang Zhang feng-Chun Zhao Yi-Bin Jiang hua feng Rong Hu Department of Neurosurgery and Key Laboratory of Neurotrauma Southwest HospitalArmy Medical UniversityChongqingChina

Brain lesions can cause neural stem cells to activate,proliferate,diffe rentiate,and migrate to the injured area.However,after traumatic brain injury,brain tissue defects and microenvironment changes greatly affect the survival and growth of neural stem cells;the resulting reduction in the number of neural stem cells impedes effective repair of the injured area.Melatonin can promote the survival,proliferation,and differentiation of neural stem cells under adverse conditions such as oxidative stress or hypoxia that can occur after traumatic brain injury.Therefore,we investigated the therapeutic effects of melatonin combined with neural stem cells on traumatic brain injury in rats.First,in vitro studies confirmed that melatonin promoted the survival of neural stem cells deprived of oxygen and glucose.Then,we established a three-dimensional Matrigel-based transplantation system containing melatonin and neural stem cells and then used it to treat traumatic brain injury in rats.We found that treatment with the Matrigel system containing melatonin and neural stem cells decreased brain lesion volume,increased the number of surviving neuro ns,and improved recove ry of neurological function compared with treatment with Matrigel alone,neural stem cells alone,Matrigel and neural stem cells combined,and Matrigel and melatonin combined.Our findings suggest that the three-dimensional Matrigelbased transplantation system containing melatonin and neural stem cells is a potential treatment for traumatic brain injury.

关键词： cell therapy magnetic resonance imaging Matrigel melatonin neural stem cells neurological function recovery three-dimensional transplantation traumatic brain injury