The effect of ecdysterone on cerebral vasospasm following experimental subarachnoid hemorrhage in vitro and in vivo
作者单位：重庆西南医院神经外科
会议名称：《中国医师协会神经外科医师分会第二届全国代表大会》
会议日期：2007年
学科分类：1002[医学-临床医学] 100204[医学-神经病学] 10[医学]
关 键 词：Cerebral vasospasm Ecdysterone Oxyhemoglobin Rabbit animal model Vascular advential fibroblasts
摘 要：正Objectives cerebral vasospasm was the dread complication of a raptured intracranial aneurysm.Worldwide effort has led to many promising experimental treatments but none were confirmed to be effective in clinical trials.Ecdysterone is a kind of insect steroid hormone.Our previous study showed that ecdysterone might prevent CVS in vitro.Even after all these studies,rare attempts have been made to test the effect of Eedysterone on VAFs,a processes known to play an important role in various pathogenic vascular conditions. Thus,we tested the hypothesis that Eedysterone could affect VAF’s characteristic and do effect in SAIl induced cerebral vasospasm.Methods 100μM OxyHb were used in the in vitro study to mimic the clinical situation,the effects of OxyHb on cells proliferation and migratio of cultured aortic smooth muscle cells was investigated.In the in vivo study,20 rabbits were equally divided into 4 groups:control group,SAH group,SAH/Nimodipine group and SAH/ecdysterone group.Changes of neurological function and cerebral angiogram of rabbits were observed after SAH.Results OxyHb increased the proliferation of VAFs at 24h.Ecdysterone co-treatment was something like to suppress proliferation.Cell cycle analysis indicated that EDS inhibited vascular advential fibroblasts from G1 to S progression.Results of migration assay showed that 100 μM OxyHb obviously prompt vascular advential fibroblasts migration and EDS would attenut this effect.In SAH/Nimodipine and SAH/ecdysterone groups,neural deficit, cerebral vasospasm and structural changes in basilar artery were alleviated with Nimodipine or ecdysterone treatment.Conclusions Ecdysterone could affect VAF’s characteristic and attenuate vasospasm after SAH.