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Plasmonic polydopamine-modified TiO_(2)nanotube substrates for surface-assisted laser desorption/ionization mass spectrometry imaging

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Nano Research 2023年第2期16卷 3028-3039页

作者： Dong Chen Mingyi Du Yudi Huang Yizhu xu Yingying Chen Lianlian Ma Qingrong Xie Xinhai Zhu Zilong Chen hanhong xu Xinzhou Wu Zhibin Yin State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources and Key Laboratory of Natural Pesticide and Chemical Biology of the Ministry of Education South China Agricultural UniversityGuangzhou 510642China Agro-biological Gene Research Center Guangdong Academy of Agricultural SciencesGuangzhou 510640China Instrumental Analysis and Research Center Sun Yat-sen UniversityGuangzhou 510275China

Mass spectrometry imaging(MSI)has made the spatio-chemical characterization of a broad range of small-molecule metabolites within biological tissues possible.However,available matrix-assisted laser desorption/ionization mass spectrometry(MALDI-MS)suffers from severe background interferences in low-mass ranges and inhomogeneous matrix deposition.Thus,surface-assisted LDI-MS(SALDI-MS)has been an attractive alternative for high-sensitivity detection and imaging of small biomolecules.In this study,we construct a new composite substrate,hydrophobic polydopamine(hPDA)-modified TiO_(2)nanotube(TDNT)coated with plasmonic gold nanoparticle(AuNP-hPDA-TDNT),as a dual-polarity SALDI substrate using an easy and cost-effective fabrication approach.Benefitting from the synergistic effects of TDNT semiconductor and plasmonic PDA modification,this SALDI substrate exhibits superior performance for dual-polarity detection of a vast diversity of small molecules.Highly reduced background interferences,lower detection limits,and spot-to-spot repeatability can be achieved using AuNP-hPDA-TDNT substrates.Due to its unique imprinting performance,various metabolites and lipids can be visualized within jatropha integerrima petals,ginkgo leaves,strawberry fruits,and latent fingerprints.More valuably,the universality of this matrix-free substrate is demonstrated for mapping spatial distribution of lipids within mouse brain tissue sections.Considered together,this AuNP-hPDA-TDNT material is expected to be a promising SALDI substrate in various fields,especially in nanomaterial development and life sciences.

关键词： TiO_(2)nanotube surface-assisted laser desorption/ionization(SALDI) mass spectrometry imaging latent fingerprint tissue imaging

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含有伯酰胺和氰基的苯基噻唑衍生物的设计、合成及杀虫活性评价

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华南农业大学学报 2024年第3期45卷 354-363页

作者：杨帅唐豪毅赵晨徐汉虹华南农业大学植物保护学院/绿色农药全国重点实验室广东广州510642

【目的】噻唑是一类具有广泛生物活性的五元杂环化合物,本文设计并合成一系列含有伯酰胺及氰基的苯基噻唑衍生物,进一步挖掘噻唑骨架在防治鳞翅目害虫领域的潜力。【方法】以不同取代基的苯甲酸为原料合成含有伯酰胺及氰基的噻唑衍生物... 详细信息

【目的】噻唑是一类具有广泛生物活性的五元杂环化合物,本文设计并合成一系列含有伯酰胺及氰基的苯基噻唑衍生物,进一步挖掘噻唑骨架在防治鳞翅目害虫领域的潜力。【方法】以不同取代基的苯甲酸为原料合成含有伯酰胺及氰基的噻唑衍生物,并经1H NMR、13C NMR和ESI-MS确证结构。采用浸液法测定目标化合物对小菜蛾Plutella xylostella的生物活性,并通过密度泛函理论(Density functional theory,DFT)计算从分子轨道层面解释该骨架具有杀虫活性的原因和差异。【结果】经结构确证,合成了22个含有伯酰胺及氰基的噻唑衍生物,其中6个化合物(6d、7d、13a、13b、14a和14b)对小菜蛾表现出很好的杀虫活性(校正死亡率>50%),尤其是化合物6d和7d,对小菜蛾的LC50达到了23.94和30.37 mg/L。构效关系表明,苯环仅取代4−CF3且5−位噻唑未取代时,表现出最优的杀虫活性。DFT计算结果表明,4−三氟甲基苯基噻唑既传递电子又接受电子,且化合物6d比7d具有更小的HOMO−LUMO间隙(ΔE)。【结论】化合物6d可作为先导化合物进一步开发,本研究可为含有噻唑骨架杀虫剂的分子设计与优化提供有价值的线索。

关键词：苯基噻唑合成杀虫活性小菜蛾密度泛函理论

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Nanoparticle-immersed paper imprinting mass spectrometry imaging reveals uptake and translocation mechanism of pesticides in plants

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Nano Research 2020年第3期13卷 611-620页

作者： Xinzhou Wu Run Qin Hanxiang Wu Guangkai Yao Yue Zhang Ping Li Yizhu xu Zhixiang Zhang Zhibin Yin hanhong xu State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources South China Agricultural UniversityGuangzhou510642China Key Laboratory of Natural Pesticide and Chemical Biology of the Ministry of Education South China Agricultural UniversityGuangzhou510642China School of Pharmaceutical Sciences Sun Yat-sen UniversityGuangzhou510006China

Design and discovery of carrier-mediated modified pesticides are vital for reducing pesticide dosage and increasing utilization,yet it remains a great challenge due to limited insights into plant translocation mechanisms.Nanostructure/nanoparticle assisted laser desorption/ionization strategy has established itself as a preferential analytical tool for biological tissue analysis,whereas potential applications in plant sciences are hindered with regard to the inability to slice plant leaves and petals.Herein,we report gold nanoparticle(AuNP)-immersed paper imprinting mass spectrometry imaging(MSI)for the spatiotemporal visualization of pesticide translocation in plant leaves.This approach plays a dual role in preserving spatial information and improving ionization efficiency for pesticides regardless of imaging artifacts due to homogenous AuNP deposition.Using this MSI platform,we proposed the elaborate plant translocation mechanism of agrochemicals for the first time,which is currently poorly understood.The dynamic processes of carrier-mediated pesticides can be clearly visualized,including crossing of plasma membranes by transporters,translocation downward in stems through the phloem,diffusion to the xylem and,conversely,accumulation at margins of the treated leaves.Moreover,this AuNP-assisted paper imprinting method could be highly compatible with laser-based MSI instruments,expediting researches across a broad range of fields,especially in nanomaterial development and life sciences.

关键词： gold nanoparticles nanoparticle-immersed paper imprinting mass spectrometry imaging plant translocation mechanism carrier-mediated pesticides

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Limonoids from seeds of Azadirachta indica A.Juss. and their cytotoxic activity

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Acta Pharmaceutica Sinica B 2018年第4期8卷 639-644页

作者： Jian Chen Xiaona Fan Jianhua Zhu Liyan Song Zhiwei Li Fei Lin Rongmin Yu hanhong xu Jiachen Zi Biotechnological Institute of Chinese Materia Medica Jinan University School of Life Sciences Sun Yat-Sen University State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources South China Agricultural University

Four new limonoid-type nortriterpenoids, 1-detigloyl-1-O-methacryloylsalannin(1), 28-deoxo-2,3-dihydronimbolide(2), 12-acetoxy-3-O-acetyl-7-O-tigloylvilasinin(3) and 12-acetoxy-3-Oacetyl-7-O-methacryloylvilasinin(4), along with five known ones, were isolated from seeds of Azadirachta indica A. Juss. Their structures were elucidated by various spectroscopic methods, including UV, IR, MS, NMR, X-ray crystallography, quantum chemical calculation, as well as by comparison of their spectroscopic data with those reported. In the in vitro cytotoxic assay, 2 showed inhibitory activity against human breast cancer MDA-MB-231 cell line with IC_(50) value of 7.68±1.74 μmol/L, and 5 inhibited growth of human cervical cancer Hela cell line, melanoma A375 cell line and promyelocytic leukemia HL-60 cell line, with IC_(50) 12.00±2.08, 17.44±2.11, and 13.95±5.74 μmol/L, respectively.

关键词： Azadirachta indica Nortriterpenoid Limonoid Spectroscopy Cytotoxic activity

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A new cytotoxic salannin-class limonoid alkaloid from seeds of Azadirachta indica A. Juss

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Chinese Chemical Letters 2018年第8期29卷 1261-1263页

作者： Jianxun Zhu Xiaofeng Lu Xiaona Fan Ruibo Wu Hongjuan Diao Rongmin Yu hanhong xu Jiachen Zi Biotechnological Institute of Chinese Materia Medica Jinan University College of Pharmacy Jinan University School of Life Sciences Sun Yat-sen University School of Pharmaceutical Science Sun Yat-sen University State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources South China Agricultural University

A new cytotoxic salannin-class limonoid alkaloid, azadiramide A（1）,was isolated from seeds of Azadirachta indica A. Juss. Its structure was elucidated by extensive analysis of spectroscopic data and quantum chemical calculation. Compound 1 is a rare salannin-class limonoid alkaloid. To our best knowledge, only two compounds belonging to this type were so far found. It showed inhibitory activity against human breast cancer MDA-MB-231 cell line with IC_（50） value of 2.70±0.63 μmol/L.

关键词： Azadirachta indica Limonoid Alkaloid Cytotoxic activity

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鸦胆因D通过影响胰岛素信号通路和诱导中肠细胞凋亡抑制黑腹果蝇生长

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江西农业大学学报 2024年第3期46卷 648-655页

作者：陈冬平李康龙诗慧田永清林菲徐汉虹华南农业大学植物保护学院广东广州510642 华南师范大学生命科学学院广东广州510631

【目的】鸦胆因D(BD)是从中药鸦胆子(Brucea javanica)中提取的植物源农药,具有良好的杀虫活性,能有效抑制包括黑腹果蝇在内的多种害虫的生长发育,但是关于BD的毒性机制研究较少,研究旨在探索BD抑制黑腹果蝇生长的毒性机制。【方法】以... 详细信息

【目的】鸦胆因D(BD)是从中药鸦胆子(Brucea javanica)中提取的植物源农药,具有良好的杀虫活性,能有效抑制包括黑腹果蝇在内的多种害虫的生长发育,但是关于BD的毒性机制研究较少,研究旨在探索BD抑制黑腹果蝇生长的毒性机制。【方法】以黑腹果蝇为研究对象,给2龄末黑腹果蝇喂食含20μg/g BD的人工饲料后,测定用药处理24 h和48 h时,BD对黑腹果蝇体质量、中肠和脂肪体的影响,通过免疫组化、蛋白质免疫印迹和实时荧光定量PCR试验,研究BD是否诱导果蝇中肠细胞凋亡、影响脂肪体细胞生长和影响营养信号通路。【结果】20μg/g BD能显著抑制黑腹果蝇的生长,体现在抑制其体质量增加和脂肪体细胞生长,同时,20μg/g BD处理48 h后,会导致果蝇脂肪体细胞核皱缩和中肠细胞凋亡。实时荧光定量PCR和蛋白质免疫印迹试验发现,BD显著上调胰岛素信号通路负调控基因4EBP和InR的转录水平,同时下调脂肪体4EBP的磷酸化水平,抑制细胞生长。【结论】BD主要通过诱导细胞凋亡和影响胰岛素信号,进而影响营养物质的消化和吸收,最终抑制黑腹果蝇的生长发育。

关键词：鸦胆因D 抑制生长凋亡 4EBP InR

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Genomic and transcriptomic analysis unveils population evolution and development of pesticide resistance in fall armyworm Spodoptera frugiperda

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Protein & Cell 2022年第7期13卷 513-531页

作者： Furong Gui Tianming Lan Yue Zhao Wei Guo Yang Dong Dongming Fang Huan Liu Haimeng Li Hongli Wang Ruoshi Hao Xiaofang Cheng Yahong Li Pengcheng Yang Sunil Kumar Sahu Yaping Chen Le Cheng Shuqi He Ping Liu Guangyi Fan Haorong Lu Guohai Hu Wei Dong Bin Chen Yuan Jiang Yongwei Zhang hanhong xu Fei Lin Bernard Slippers Alisa Postma Matthew Jackson Birhan Addisie Abate Kassahun Tesfaye Aschalew Lemma Demie Meseret Destaw Bayeleygne Dawit Tesfaye Degefu Feng Chen Paul K.Kuria Zachary M.Kinyua Tong-Xian Liu Huanming Yang Fangneng Huang Xin Liu Jun Sheng Le Kang State Key Laboratory for Conservation and Utilization of Bioresources in Yunnan Yunnan Agricultural UniversityKunming 650201China State Key Laboratory of Agricultural Genomics BGI-ShenzhenShenzhen 518083China State Key Laboratory of Integrated Management of Pest Insects and Rodents Institute of ZoologyChinese Academy of SciencesBeijing 100101China Beijing Institutes of Life Science Chinese Academy of SciencesBeijing 100101China MGI BGI-ShenzhenShenzhen 518083China BGI-Qingdao BGI-ShenzhenQingdao 266555China BGI-Yunnan No.389 Haiyuan RoadHigh-tech Development ZoneKunming 650106China China National GeneBank Jinsha RoadDapeng New DistrictShenzhen 518120China Department of Biology University of Copenhagen2100 CopenhagenDenmark Guangdong Provincial Key Laboratory of Genome Read and Write BGI-ShenzhenShenzhen 518120China Guangdong Provincial Academician Workstation of BGI Synthetic Genomics BGI-ShenzhenShenzhen 518120China Yunnan Plateau Characteristic Agriculture Industry Research Institute Kunming 650201China College of Plant Health and Medicine Qingdao Agricultural UniversityQingdao 266109China Department of Entomology Louisiana State University Agricultural CenterBaton RougeLA 70803USA CAS Center for Excellence in Biotic Interactions University of Chinese Academy of SciencesBeijing 100101China Yunnan Plant Protection and Quarantine Station Kunming 650034China State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources South China Agricultural UniversityGuangzhou 510642China BGI-Americas One Broadway14th FloorCambridgeMA 02142USA Departme nt of Biochemistry Gen etics and MicrobiologyForestry and Agricultural Biotech no logy InstituteUn iversity of PretoriaPretoriaSouth Africa Ethiopian Biotechnology Institute Addis AbabaEthiopia College of Natural Science Addis Ababa UniversityAddis AbabaEthiopia Melkassa Agricultural Research Center Ethiopian Institute of Agricultural ResearchMelkassaAddis AbabaEthiopia Kenya Agricultural and Livestock Res

The fall armyworm(FAW),Spodoptera frugiperda,is a destructive pest native to America and has recently become an invasive insect pest in China.Because of its rapid spread and great risks in China,understanding of FAW genetic background and pesticide resistance is urgent and essential to develop effective management strategies.Here,we assembled a chromosome-level genome of a male FAW(SFynMstLFR)and compared re-sequencing results of the populations from America,Africa,and China.Strain identification of 163 individuals collected from America,Africa and China showed that both C and R strains were found in the American pop-ulations,while only C strain was found in the Chinese and African populations.Moreover,population geno-mics analysis showed that populations from Africa and China have close relationship with significantly genetic differentiation from American populations.Taken toge-ther,FAWs invaded into China were most likely origi-nated from Africa.Comparative genomics analysis displayed that the cytochrome p450 gene family is extremely expanded to 425 members in FAW,of which 283 genes are specific to FAW.Treatments of Chinese populations with twenty-three pesticides showed the variant patterns of transcriptome profiles,and several detoxification genes such as AOX,UGT and GST spe-cially responded to the pesticides.These findings will be useful in developing effective strategies for manage-ment of FAW in China and other invaded areas.

关键词： Spodoptera frugiperda chromosome-level genome population differentiation cytochrome p450 pesticides

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皂角发酵物对红托竹荪病害防治及土壤主要微生物群落影响

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菌物学报 2022年第4期41卷 618-629页

作者：张雪张学学胡鑫徐汉虹田永清亚热带农业生物资源保护与利用国家重点实验室天然农药与化学生物学教育部重点实验室华南农业大学植物保护学院广东广州510642

为了研究皂角发酵物对贵州当地特色产业红托竹荪的病害防治及促生长作用,本研究在室内分离病原菌并进行平板对峙实验,田间试验设计4个处理:不施用药剂的常规处理、解淀粉芽孢杆菌HN11菌液、皂角粉末、皂角发酵物,调查防治效果,测量菌蛋... 详细信息

为了研究皂角发酵物对贵州当地特色产业红托竹荪的病害防治及促生长作用,本研究在室内分离病原菌并进行平板对峙实验,田间试验设计4个处理:不施用药剂的常规处理、解淀粉芽孢杆菌HN11菌液、皂角粉末、皂角发酵物,调查防治效果,测量菌蛋大小及个数,检测土壤微生物群落变化。从发病组织中分离出一株病原菌,鉴定为阴沟肠杆菌Enterobacter cloacae。皂角发酵物对竹荪病害田间防治效果达77.86%,生长面积提高61.22%。土壤微生物群落中细菌和真菌分析结果显示,相较于其他处理组,皂角发酵物处理组中竹荪相对丰度占比最大,达25.83%。皂角发酵物能有效防治红托竹荪病害,促进红托竹荪菌蛋生长,减少化学农药的用量,促进生态循环,保障农产品食用安全,提升皂角和竹荪产业综合效益。

关键词：皂角发酵物红托竹荪阴沟肠杆菌促生长土壤微生物

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分子对接阐明草甘膦与水稻醛酮还原酶(OsALR2)的作用及OsALR2的表达纯化

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植物保护 2023年第6期49卷 23-31页

作者：孙跃刘蓉王思威徐汉虹吴鹰花华南农业大学天然农药与化学生物学教育部重点实验室广州510642 中山榄菊日化实业有限公司广东省博士后创新实践基地中山528400

草甘膦是一种广谱高效的有机磷类除草剂,因其对水稻等禾本科作物缺乏选择性,导致其在水稻田中的应用受到限制。杂草中醛酮还原酶(aldo-keto reductase,AKR)具有降解小分子农药草甘膦的功能,但是水稻响应草甘膦的作用机制未知。本文通过... 详细信息

草甘膦是一种广谱高效的有机磷类除草剂,因其对水稻等禾本科作物缺乏选择性,导致其在水稻田中的应用受到限制。杂草中醛酮还原酶(aldo-keto reductase,AKR)具有降解小分子农药草甘膦的功能,但是水稻响应草甘膦的作用机制未知。本文通过生物信息学分析、分子对接研究草甘膦与其在水稻中的靶标AKR蛋白OsALR2的互作机制。采用TMHMM 2.0和DiANNA 1.1 web server等在线软件对OsALR2蛋白的跨膜特性和二硫键含量等生物学特性进行了分析,结果表明OsALR2分子量为35 kD,等电点为5.89;OsALR2不是膜蛋白,但是含有3组二硫键。使用AlphaFold预测OsALR2蛋白的三级结构,并利用Auto Dock Vina将草甘膦与OsALR2进行分子对接,结果显示,最低结合能为-13.4 kcal/mol,草甘膦与OsALR2的结合主要通过氢键作用。通过Ramachandran图,ERRAT和Verify 3D证明对接获得的三维结构是合理的。进一步通过分子克隆技术构建重组表达载体pET-32a-OsALR2并进行原核表达,结果显示OsALR2主要以包涵体的形式表达,对包涵体进行变复性后,蛋白表现出酶活性且蛋白结构有较好的均一性。本文的研究结果助于阐明草甘膦与水稻蛋白互作的分子机制,为研发抗草甘膦水稻新品种和新型小分子农药提供理论指导。

关键词：水稻草甘膦分子对接原核表达包涵体变复性酶活测定

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皂荚提取物的杀螺活性

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生物安全学报 2022年第4期31卷 375-379页

作者：张学学张雪徐楷杰胡鑫徐汉虹田永清亚热带农业生物资源保护与利用国家重点实验室/天然农药与化学生物学教育部重点实验室华南农业大学广东广州510642

【目的】研究皂荚生物农药活性,开发利用皂荚资源,发展环境友好的绿色植物源农药。【方法】采用室内生测和田间试验研究皂荚壳乙醇提取物的杀螺活性。【结果】皂荚提取物对福寿螺有显著的毒杀活性,对幼螺和成螺72 h的LC_(50)分别为40.56... 详细信息

【目的】研究皂荚生物农药活性,开发利用皂荚资源,发展环境友好的绿色植物源农药。【方法】采用室内生测和田间试验研究皂荚壳乙醇提取物的杀螺活性。【结果】皂荚提取物对福寿螺有显著的毒杀活性,对幼螺和成螺72 h的LC_(50)分别为40.56、109.83 mg·L^(-1)。田间试验表明,皂荚提取物对福寿螺有较好的防效,施用40 g·m^(-2)的皂荚提取物处理7 d后卵块减少率为100.00%(成螺失去产卵的能力),防效为(99.12±1.26)%。【结论】皂荚提取物对福寿螺较好的生物防治效果,是一种潜在的生物杀螺剂。

关键词：皂荚福寿螺杀螺活性田间防效

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