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Phyto.hemical profiling and anticancer po.ential o. Cymbo.o.o. citratus extract

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Asian Pacific Jo.rnal o. Tro.ical biomedicine 2024年第10期14卷 448-460页

作者： Bader o.Almutairi Mikhlid H.Almutairi Badr A.Al-Dahmash Saad Alkahtani Saud Alarifi Ahmed Rady Department o. Zo.lo.y College of ScienceKing Saud UniversityP.O.Box 2455Riyadh 11451Saudi Arabia

o.jective:To.evaluate the anticancer po.ential o. Cymbo.o.o. citratus ***:GC-MS analysis was used to.identify phyto.o.po.ents in the methano.ic extract o. Cymbo.o.o. citratus.A fractio.atio. metho. was emplo.ed to.iso.ate and assess the bioactivity o. different fractio.s and their cyto.o.ic activities against cancer cell lines HCT116,Lo.o.Caco.2,and HT-29 were investigated.A dual staining metho. with acridine o.ange and ethidium bro.ide was used to.assess the effect o. the extract o. cell ***,the expressio. levels o. Bax and TP53 were quantified using real-time PCR in Caco.2 cells treated with the ethyl acetate fractio. o. Cymbo.o.o. citratus extract.A protein array was emplo.ed to.profile key pro-and anti-apo.to.ic proteins in Caco.2 ***,mo.ecular do.king studies were co.ducted to.investigate the interactio.s between key co.po.nds o. Cymbo.o.o. citratus extract and specific apo.to.is-related protein do.ains(PDB IDs:7wql and 4bkx).Results:A significant gro.th inhibitio. was o.served in Caco.2 cells treated with Cymbo.o.o. citratus *** the seven fractio.s o. the plant extract,the ethyl acetate fractio. sho.ed the highest cyto.o.icity against Caco.2 cells with an IC50 value o.(6.16±0.01)μg/*** immuno.luo.escence assay sho.ed that the ethyl acetate fractio. co.ld induce apo.to.is o. Caco.2 ***,the fractio. upregulated the gene expressio.s o. Bax and TP53 in a do.e-dependent *** do.king analysis demo.strated the interactio. o. five co.po.nds iso.ated fro. the ethyl acetate fractio. with key proteins in Caco.2 cells,indicating their anticancer ***:Cymbo.o.o. citratus extract sho.s anticancer activity against Caco.2 cells by inducing *** may be a promising candidate fo. the treatment o. co.o. cancer,which needs further investigatio..

关键词： Cymbo.o.o. citratus Anticancer Caco.2 cell Apo.to.is Co.o. cancer

Guided wave propagatio. in multilayered piezo.lectric structures

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Science china(Physics,Mechanics & Astro.o.y) 2009年第7期52卷 1094-1104页

作者： GUo.yo.gQiang1, CHEN WeiQiu2,3 & ZHANG yo.gLiang4 1 key labo.ato.y o. Mechanics o. Disaster and Enviro.ment in Western china, Ministry o. Educatio., and Scho.l o. Civil Engineering and Mechanics, Lanzho. university, Lanzho. 730000, china 2 key labo.ato.y o. So.t So.ls and Geo.nviro.mental Engineering, Ministry o. Educatio., and Department o. Civil Engineering, zhejiang university, hangzho. 310058, china 3 Department o. Engineering Mechanics, zhejiang university, hangzho. 310027, china 4 Scho.l o. Civil Engineering, Lanzho. Jiao.o.g university, Lanzho. 730070, china key labo.ato.y o. Mechanics o. Disaster and Enviro.ment in Western china Ministry of Education and School of Civil Engineering and Mechanics Lanzhou University Lanzhou China key labo.ato.y o. So.t So.ls and Geo.nviro.mental Engineering Ministry of Education and Department of Civil Engineering Zhejiang University Hangzhou China Department o. Engineering Mechanics Zhejiang University Hangzhou China Scho.l o. Civil Engineering Lanzhou Jiaotong University Lanzhou China

A general fo.mulatio. o. the metho. o. the reverberatio.-ray matrix (MRRM) based o. the state space fo.malism and plane wave expansio. technique is presented fo. the analysis o. guided waves in multilayered piezo.lectric structures. Each layer o. the structure is made o. an arbitrarily aniso.ro.ic piezo.lectric material. Since the state equatio. o. each layer is derived fro. the three-dimensio.al theo.y o. linear piezo.lectricity, all wave mo.es are included in the fo.mulatio.. Within the framewo.k o. the MRRM, the phase relatio. is properly established by excluding expo.entially gro.ing functio.s, while the scattering relatio. is also.appropriately set up by avo.ding matrix inversio. o.eratio.. Co.sequently, the present MRRM is unco.ditio.ally numerically stable and free fro. co.putatio.al limitatio.s to.the to.al number o. layers, the thickness o. individual layers, and the frequency range. Numerical examples are given to.illustrate the go.d perfo.mance o. the propo.ed fo.mulatio. fo. the analysis o. the dispersio. characteristic o. waves in layered piezo.lectric structures.

关键词： multilayered piezo.lectric structures the metho. o. reverberatio.-ray matrix guided waves dispersio. characteristic

Po.itive Darwinian selectio. in human po.ulatio.:A review

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Chinese Science Bulletin 2008年第10期53卷 1457-1467页

作者： WU Do.gDo.g1,3 & ZHANG yaPing1,2 1 State key labo.ato.y o. Genetic Reso.rces and Evo.utio., Kunming institute o. Zo.lo.y, Chinese Academy o. Sciences, Kunming 650223, china 2 labo.ato.y fo. Co.servatio. and Utilizatio. o. bio-reso.rce, yunnan university, Kunming 650091, china 3 Graduate university o. Chinese Academy o. Sciences, Beijing 100049, china State key labo.ato.y o. Genetic Reso.rces and Evo.utio. Kunming Institute of Zoology Chinese Academy of Sciences Kunming China Graduate university o. Chinese Academy o. Sciences Beijing China labo.ato.y fo. Co.servatio. and Utilizatio. o. bio-reso.rce Yunnan University Kunming China

This paper reviews a large number o. genes under po.itive Darwinian selectio. in mo.ern human po.ulatio.s, such as brain develo.ment genes, immunity genes, reproductive related genes, perceptio. recepto.s. The research o. the evo.utio.ary property o. these genes will provide impo.tant insight into.human evo.utio. and disease mechanisms. With the increase o. po.ulatio. genetics and co.parative geno.ics data, mo.e and mo.e evidences indicate that po.itive Darwinian selectio. plays an indispensable ro.e in the o.igin and evo.utio. o. human beings. This paper will also.summarize the metho.s to.detect po.itive selectio., analyze the interference facto.s faced and make suggestio.s fo. further research o. po.itive selectio..

关键词：自然选择积极选择达尔文进化论人类学种群遗传

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N-硝基精氨酸抑制海马神经元兴奋机制

生物化学与生物物理学报 2001年第4期33卷 437-442页

作者：陈啸春盛超郑筱祥浙江大学生物医学工程系!浙江省心脑血管、神经系统药物筛选和中药新药开发及评价研究重点实验室杭州310027

研究N 硝基精氨酸 (L NNA)在青霉素诱发的培养海马CA1区神经元细胞兴奋过程中的抑制机制。用激光扫描共聚焦显微镜观察发现 40 0 0IU/ml的青霉素可诱发一氧化氮 (No.的快速合成模式。L NNA(0～ 10 μmo./L)呈剂量依赖性抑制No.合成和... 详细信息

研究N 硝基精氨酸 (L NNA)在青霉素诱发的培养海马CA1区神经元细胞兴奋过程中的抑制机制。用激光扫描共聚焦显微镜观察发现 40 0 0IU/ml的青霉素可诱发一氧化氮 (No.的快速合成模式。L NNA(0～ 10 μmo./L)呈剂量依赖性抑制No.合成和青霉素刺激 15min后谷氨酸水平的二次升高。同时发现 1μmo./L和 10 μmo./L剂量的L NNA可显著抑制甲硫氨酸脑啡肽的水平 ,而 10 μmo./L剂量的L NNA还显著升高强啡肽 B的水平。对L NNA抑制谷氨酸水平二次升高效应 ,10 0 μmo./L的甲硫氨酸脑啡肽受体抑制剂 β funaltrexamine(β FNA)可显著协同 ,而 10 0 μmo./L的强啡肽 B受体抑制剂no.binalto.phimine(no. BIN)则显著逆转。以上结果提示 :L NNA抑制 40 0 0IU/ml的青霉素诱导的No.成 ,并进而通过抑制甲硫氨酸脑啡肽水平 ,升高强啡肽 B水平来抑制谷氨酸水平 ,调节神经元的兴奋性

关键词：一氧化氮谷氨酸脑啡肽强啡肽海马神经元

Fluo.escence Reso.ance Energy Transfer Analysis o. Bid Activatio. in Living Cells during Ultravio.et-induced Apo.to.is

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Acta biochimica et biophysica Sinica 2007年第1期39卷 37-45页

作者： yinyuan WU Da XING Lei LIU To.gsheng CHEN and Wei R.CHEN Mo. key labo.ato.y o. Laser Life Science &institute o. Life Science,So.th china No.mal university,Guangzho. 510631,china Mo. key labo.ato.y o. Laser Life Science & institute o. Laser Life Science South China Normal UniversityGuangzhou 510631 China

Ultravio.et (UV) irradiatio. is a DNA-damaging agent that triggers apo.to.is thro.gh bo.h themembrane death recepto. and mito.ho.drial apo.to.ic signaling ***,a pro-apo.to.ic Bcl-2family member,is impo.tant in mo.t cell types to.apo.to.is in respo.se to.DNA *** this study,areco.binant plasmid,yFP-Bid-CFP,co.prised o. yello. and cyan fluo.escent protein and a full length Bid,was used as a fluo.escence reso.ance energy transfer analysis (FRET) *** the FRET techniquebased o. yFP-Bid-CFP,we fo.nd that Bid activatio. was initiated at 9±1 h after UV irradiatio.,and theaverage duratio. o. the activatio. was 75±10 *** activatio. co.ncided with a co.lapse o. the mito.ho.drialmembrane po.ential with an average duratio. o. 50±10 min. When cells were pretreated with Z-IETD-fmk(caspase-8 specific inhibito.) the process o. Bid activatio. was co.pletely inhibited,but the apo.to.is waso.ly partially affected.Z-DEVD-fmk (caspase-3 inhibito.) and Z-FA-fmk (no. asp specific inhibito.) didno. blo.k Bid ***,the endo.3}us Bid activatio. with o. witho.t Z-IETD-fmk in respo.seto.UV irradiatio. was co.firmed by Western *** summary, using the FRET technique,we o.servedthe dynamics o. Bid activatio. during UV-induced apo.to.is and fo.nd that it was a caspase-8 dependentevent.

关键词： ultravio.et irradiatio. Bid activatio. apo.to.is caspase-8 fluo.escence reso.ance energy transfer analysis

Interactio. o. cellular-lo.alized signature mo.ules in respo.se to.prostate cancer

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progress in Natural Science:Materials Internatio.al 2007年第11期17卷 1368-1375页

作者： Zhu Jing1, Guo.Zheng1,2, Zhang Min2, yang Da2, Wang Jing2 and Wang Chenguang2(1. Scho.l o. Life Science and bio.nfo.matics Centre, university o. Electro.ic Science and Techno.o.y o. china, Chengdu 610054, china 2. Department o. bio.nfo.matics, bio-Pharmaceutical key labo.ato.y o. Heilo.gjiang pro.ince, Harbin Medical university, Harbin 150081, china) Scho.l o. Life Science and bio.nfo.matics Centre University of Electronic Science and Technology of China Chengdu 610054 China bio-Pharmaceutical key labo.ato.y o. Heilo.gjiang pro.ince Department of Bioinformatics Harbin Medical University Harbin 150081 China

Rapid progress in high-thro.ghput biotechno.o.ies (e.g. micro.rrays) and expo.ential accumulatio. o. gene functio.al kno.ledge makes it promising fo. systematic understanding o. co.plex human diseases at the functio.al mo.ules level. Current mo.ular catego.izatio.s can be defined and selected mo.e specifically and precisely in terms o. bo.h biolo.ical processes and cellular lo.atio.s, aiming at unco.ering the mo.ular mo.ecular netwo.ks highly relevant to.cancers. Based o. Gene o.to.o.y, we identifed the functio.al mo.ules enriched with differentially expressed genes and characterized by biolo.ical processes and specific cellular lo.atio.s. Then, acco.ding to.the ranking o. the disease discriminating abilities o. the pre-selected functio.al mo.ules, we further defined and filtered signature mo.ules which have higher relevance to.the cancer under study. Applicatio.s o. the propo.ed metho. to.the analysis o. a prostate cancer dataset revealed insightful biolo.ical mo.ules.

关键词： micro.rray analysis Gene o.to.o.y classificatio. analysis cellular lo.atio. functio.al mo.ule

Evo.utio. o. cd59 gene in mammals

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Science china(Life Sciences) 2007年第6期50卷 773-779页

作者： Go.G yuanying1,2, PENG MinSheng1,2, ZHo. WeiPing1,3,4 & ZHANG yaPing1,4 1 State key labo.ato.y o. Genetic Reso.rces and Evo.utio., Kunming institute o. Zo.lo.y, Chinese Academy o. Sciences, Kunming 650223, china 2 Graduate university o. the Chinese Academy o. Sciences, Beijing 100049, china 3 Mo.ecular and Cell biolo.y, university o. Science and Techno.o.y o. china, Hefei 230026, china 4 labo.ato.y fo. Co.servatio. and Utilizatio. o. bio-reso.rces, yunnan university, Kunming 650091, china State key labo.ato.y o. Genetic Reso.rces and Evo.utio. Kunming Institute of Zoology Chinese Academy of Sciences Kunming China Graduate university o. the Chinese Academy o. Sciences Beijing China Mo.ecular and Cell biolo.y University of Science and Technology of China Hefei China labo.ato.y fo. Co.servatio. and Utilizatio. o. bio-reso.rces Yunnan University Kunming China

The CD59-co.ing sequences were o.tained fro. 5 mammals by PCR and BLAST, and co.bined with the available sequences in GenBank, the nucleo.ide substitutio. rates o. mammalian cd59 were calcu- lated. Results o. syno.ymo.s and no.syno.ymo.s substitutio. rates revealed that cd59 experienced negative selectio. in mammals o.erall. Fo.r sites experiencing po.itive selectio. were fo.nd by using "site-specific" mo.el in PAML so.tware. These sites were distributed o. the mo.ecular surface, o. which 2 sites lo.ated in the key functio.al do.ain. Furthermo.e, "branch-site-specific" mo.el detected 1 po.itive site in cd59a and cd59b lineages which underwent accelerated evo.utio. caused by po.itive selectio. after gene duplicatio. in mo.se.

关键词： cd59, evo.utio., po.itive selectio.

Effects o. Cu^(2+) o. characteristic o. SV currents

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Science china Chemistry 2006年第6期49卷 560-564页

作者： ZHANG Liping1,2 & yANG Pin1 1. key labo.ato.y o. Chemical biolo.y and Mo.ecular Engineering o. Ministry o. Educatio., institute o. Mo.ecular Science, Shanxi university, Taiyuan 030006, china 2. institute o. Enviro.ment Science, Taiyuan university o. Science and Techno.o.y, Taiyuan 030024, china key labo.ato.y o. Chemical biolo.y and Mo.ecular Engineering o. Ministry o. Educatio. Institute of Molecular Science Shanxi University Taiyuan China institute o. Enviro.ment Science Taiyuan University of Science and Technology Taiyuan China

Applying the patch-clamp technique to.vacuo.es fro. Radish we studied the effects o. Cu2+ o. Slo. Vacuo.ar (SV) current’s characteristic. o.r results sho. that Cu2+ in bath so.utio. at higher co.centratio. inhibits SV currents and the percentage o. inhibitio. increases with increasing co.centratio. and changes with different vo.tage. When at lo.er co.centratio., Cu2+ significantly promo.es the SV currents and the promo.io. ratio.decrease with increasing vo.tage. At the same time, the time co.stants o. activatio. beco.e lesser after adding Cu2+. These results sho. that there may be so.e Cu2+ binding sites o. SV channels and binding to.which can change SV current’s characteristic.

关键词： the patch-clamp technique, vacuo.ar, Cu2+, SV channel.

A draft sequence o. the rice (o.yza sativa ssp. indica) genome

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Chinese Science Bulletin 2001年第23期46卷 1937-1942页

作者： yU Jun, HU So.gnian, WANG Jun,LI So.ggang Wo.G Ka-Shu Gane, LIU Bin,DENG yajun, DAI Li, ZHo. yan,ZHANG Xiuqing, CAo.Mengliang, LIU Jing,SUN Jiando.g , TANG Jiabin, CHEN yanjio.g,HUANG Xiao.ing, LIN Wei, yE Chen, To.G Wei,Co.G Lijuan, GENG Jianing, HAN yujun, LI Lin,LI Wei, HU Guangqiang, HUANG Xiangang,LI Wenjie, LI Jian, LIU Zhanwei, LI Lo.g,LIU Jianping, Ql Qiuhui, LIU Jinso.g, LI Li,WANG Xuegang, LU Ho.g, WU Tingling,ZHU Miao. Nl Peixiang, HAN Hua, Do.G Wei,REN Xiao.u, FENG Xiao.i, GUI Peng,LI Xianran, WANG Hao. XU Xin, ZHAI Wenxue,XU Zhao. ZHANG Jinso.g, HE Sijie,ZHANG Jianguo. XU Jichen, ZHANG Kunlin,ZHENG Xianwu, Do.G Jianhai, ZENG Wanyo.g,TAo.Lin, CHEN Xuewei, HE Jun, LIU Dao.eng,TIAN Wei, TIAN Chao.uang, XIA Ho.gai,LI Gang, GAo.Hui, LI Ping, CHEN Wei ,WANG Xudo.g, ZHANG yo.g, HU Jianfei,WANG Jing, LIU So.g, yANG Jian,ZHANG Guangyu, XIo.G yuqing, LI Zhijie,MAo.Lo.g, ZHo. Chengshu, ZHU Zhen,CHEN Runsheng, HAo.Bailin,ZHENG Weimo., CHEN Sho.yi, QUo.Wei,LI Guo.ie, LIU Siqi, HUANG Guyang,TAo.Ming, WANG Jian, ZHU Lihuang,yUAN Lo.gping& yANG HuanmingBeijing geno.ics institute/Center o. geno.ics & bio.nfo.matics, Chinese Academy o. Sciences, Beijing 101300, china hangzho. geno.ics institute/institute o. bio.nfo.matics o. zhejiang university/key labo.ato.y o. bio.nfo.matics o. zhejiang pro.ince, hangzho. 310007, china institute o. Genetics, Chinese Academy o. Sciences, Beijing 100101, china Natio.al Hybrid Rice R & D Center, Changsha 410125, china labo.ato.y o. bio.nfo.matics, institute o. biophysics, Chinese Academy o. Sciences, Beijing 100101, china Co.lege o. Life Sciences, Peking university, Beijing 100871, china institute o. Theo.etical Physics, Chinese Academy o. Sciences, Beijing 1Q0080, china Digital china Ltd., Beijing 100080, china institute o. Co.puting Techno.o.y, Chinese Academy o. Sciences, Beijing 100080, china Medical Co.lege, Xi’an Jiao.o.g university, Xi’an 710061, chinaThese autho.s co.tributed equally to.this wo.k.Co.respo.ding autho..Co.respo.den Beijing geno.ics institute/Center o. geno.ics & bio.nfo.matics Chinese Academy of Sciences Beijing China hangzho. geno.ics institute/institute o. bio.nfo.matics o. zhejiang university/key labo.ato.y o. bio.nfo.matics o. zhejiang pro.ince Hangzhou China Co.lege o. Life Sciences Peking University Beijing China Medical Co.lege Xi’an Jiaotong University Xi’an China institute o. Genetics Chinese Academy of Sciences Beijing China labo.ato.y o. bio.nfo.matics Institute of Biophysics Chinese Academy of Sciences Beijing China Natio.al Hybrid Rice R & D Center Changsha China institute o. Theo.etical Physics Chinese Academy of Sciences Beijing China Digital china Ltd. Beijing China institute o. Co.puting Techno.o.y Chinese Academy of Sciences Beijing China

The sequence o. the rice genome ho.ds fundamental informatio. fo. its biolo.y, including physio.o.y, genetics, develo.ment, and evo.utio., as well as informatio. o. many beneficial pheno.ypes o. eco.o.ic significance. Using a "who.e genome sho.gun" approach, we have pro-duced a draft rice genome sequence o. o.yza sativa ssp. in-dica, the majo. cro. rice subspecies in china and many o.her regio.s o. Asia. The draft genome sequence is co.structed fro. o.er 4.3 millio. successful sequencing traces with an accumulative to.al length o. 2214.9 Mb. The initial assembly o. the no.-redundant sequences reached 409.76 Mb in length, based o. 3.30 millio. successful sequencing traces with a to.al length o. 1797.4 Mb fro. an indica variant cultivar 93-11, giving an estimated co.erage o. 95.29% o. the rice genome with an average base accuracy o. higher than 99%. The co.erage o. the draft sequence, the rando.ness o. the sequence distributio., and the co.sistency o. BIG-ASSEM-BLER, a custo.-designed so.tware package

关键词： rice genome draft sequences.