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Molecular identification of the potentially forensically relevant cluster flies Pollenia rudis (Fabricius) and Pollenia vagabunda (Meigen) (Diptera: Polleniidae) — non-recorded species in Algeria

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Forensic Sciences Research 2022年第1期7卷 69-77页

作者： Meriem Taleb Ghania Tail halide nihal açıkgöz Department of Population and Organism Biology University of Blida 1BlidaAlgeria Department of Forensic Biology Ankara UniversityMamakAnkaraTurkey

Cluster flies are represented by the genus Pollenia Robineau-Desvoidy,1830 of the family Polleniidae Brauer and Bergenstamm,*** larvae are known to be internal parasites or predators of ***,we report for the first time the occurrence of the cluster flies Pollenia rudis Fabricius,1794 and Pollenia vagabunda(Meigen,1826)(Diptera:Polleniidae)on carcasses in Algeria and identify them through DNA barcoding.A region of the mitochondrial cytochrome c oxidase I gene(COI)was amplified and *** distances were determined.A phylogenetic tree was constructed with the maximum parsimony method using 10000 bootstrap replicates.A total number of 157 adults of *** were collected together with 325 adults of Pollenia *** occurrence of Pollenia on animal carcasses does not seem to be correlated with a particular stage of *** the sequences were correctly identified using the BLASTn tool from the GenBank database and the BOLD identification ***-and interspecific sequence divergence values were less than 1%and greater than 3%,*** barcodes obtained from this study were robust enough to identify and distinguish unambiguously between *** and *** the tree-based analysis,the cluster flies were all assigned to their respective species separately from each other confirming the morphological *** results provide DNA barcodes that contribute to the growth of reference databases and allow fast and accurate identification.

关键词： Forensic sciences forensic entomology Pollenia cytochrome c oxidase I DNA barcoding North Africa

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