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Identification and Characterization of a Novel Single Domain Antibody Against Ebola Virus

Virologica Sinica 2021年第6期36卷 1600-1610页

作者： Rui Wang haiwei zhang Cheng Peng Jian Shi Huajun zhang Rui Gong CAS Key Laboratory of Special Pathogens and Biosafety Wuhan Institute of VirologyCenter for Biosafety Mega-ScienceChinese Academy of SciencesWuhan430071China University of Chinese Academy of Sciences Beijing100049China

Ebola virus(EBOV) belongs to the Filoviridae family and causes severe illnesses such as hemorrhagic fever with a high mortality rate up to 90%. Now two antibody drugs termed Inmazeb and Ebanga have been approved for treating EBOV infection. However, clinical studies have demonstrated that the mortality rate of the patients who received these two antibody drugs remains above 30%. Therefore, novel therapeutics with better efficacy is still desired. The isolated human IgG1 constant domain 2(CH2 domain) has been proposed as a scaffold for the development of C-based single domain antibodies(C-sd Abs) as therapeutic candidates against viral infections and other diseases. Here, we screened and identified a novel C-sd Ab termed M24 that targets EBOV glycoprotein(GP) from a C-sd Ab phage display library. M24 neutralizes the pseudotype EBOV with IC;of 0.8 nmol/L(12 ng/mL) and has modest neutralizing activity against authentic *** determination, including molecular docking and site mutation analysis, discloses that M24 binds to the internal fusion loop(IFL) within GP2, a transmembrane subunit of GP. Interestingly, we found that the binding of M24 to GP at pH5.5 has dramatically decreased compared to the binding at pH 7.5, which may lead to weak efficacy in the neutralization of authentic EBOV. Since no sd Ab against EBOV infection has been reported to date, our results not only give a proof of concept that sd Abs could be utilized for the development of potential therapeutic candidates against EBOV infection, but also provide useful information for the discovery and improvement of anti-EBOV agents.

关键词： Ebola virus(EBOV) Constant domain 2(CH2 domain) C-based single domain antibody(C-sdAb) Neutralize Fusion loop

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Rapamycin promotes Schwann cell migration and nerve growth factor secretion

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Neural Regeneration Research 2014年第6期9卷 602-609页

作者： Fang Liu haiwei zhang Kaiming zhang Xinyu Wang Shipu Li Yixia Yin Department of Orthopedics Second Hospital of Yueyang State Key Laboratory of Advanced Technology for Materials Synthesis and Processing and Biomedical Materials and Engineering Center Wuhan University of Technology

Rapamycin, similar to FKS06, can promote neural regeneration in vitro. We assumed that the mechanisms of action of rapamycin and FK506 in promoting peripheral nerve regeneration were similar. This study compared the effects of different concentrations of raparnycin and FK506 on Sc hwann cells and investigated effects and mechanisms of rapamycin on improving peripheral nerve regeneration. Results demonstrated that the lowest rapamycin concentration （1.53 nmol/L） more significantly promoted Schwann cell migration than the highest FK506 concentration （100μmol/L）. Rapamycin promoted the secretion of nerve growth factors and upregulated growth-associated protein 43 expression in Schwann cells, but did not significantly affect Schwann cell proliferation. Therefore, rapamycin has potential application in peripheral nerve regeneration therapy.

关键词： nerve regeneration peripheral nerve injury rapamycin FK506 Schwann cell cell mi-gration nerve growth factor growth-associated protein 43 NSFC grant neural regeneration

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Genotypic variation in phosphorus acquisition from sparingly soluble P sources is related to root morphology and root exudates in Brassica napus

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Science China(Life Sciences) 2011年第12期54卷 1134-1142页

作者： zhang haiwei HUANG Yu YE XiangSheng XU FangSen National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University Wuhan 430070 China Key Laboratory of Subtropical Agriculture and Environment Ministry of Agriculture Huazhong Agricultural University Wuhan 430070 China

Genotypic variations in the adaptive response to low-phosphorus （P） stress and P-uptake efficiency have been widely reported in many crops. We conducted a pot experiment to evaluate the P-acquisition ability of two rapeseed （Brassica napus） geno- types supplied with two sparingly soluble sources of P, A1-P and Fe-P. Then, the root morphology, proton concentrations, and carboxylate content were investigated in a solution experiment to examine the genotypic difference in P-acquisition efficiency. Both genotypes produced greater biomass and accumulated more P when supplied with A1-P than when supplied with Fe-P. The P-efficient genotype 102 showed a significantly greater ability to deplete sparingly soluble P from the rhizosphere soil be- cause of its greater biomass and higher P uptake compared with those of the P-inefficient genotype 105. In the solution exper- iment, the P-efficient genotype under low-P conditions developed dominant root morphological traits, and it showed more in- tensive rhizosphere acidification because of greater H＋ effiux, higher H＋-ATPase activity, and greater exudation of carbox- ylates than the P-inefficient genotype. Thus, a combination of morphological and physiological mechanisms contributed to the genotypic variation in the utilization of different sparingly soluble P sources in B. napus.

关键词： Brassica napus sparingly soluble P genotypic variation root morphology H+ and carboxylate exudation

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Chinese stalagmite paleoclimate researches: A review and perspective

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Science China Earth Sciences 2019年第10期62卷 1489-1513页

作者： Hai CHENG haiwei zhang Jingyao ZHAO Hanying LI Youfeng NING Gayatri KATHAYAT Institute of Global Environmental Change Xi’an Jiaotong University

Stalagmite is one kind of secondary carbonates formed in limestone caves(speleothem). After cave water droplets containing Ca2+and HCO3 drip onto floor, carbonate in the water might become supersaturated due to CO2 degassing under certain conditions, resulting in the formation of stalagmite in a process year after year. Stalagmite is one of important geological archives for paleoclimate research. The advantages include wide spatial distribution, suitable for U-Th and U-Pb dating, enriched in climate proxies, continuity, long time span, comparability and lower sampling cost etc. These factors have propelled stalagmite paleoclimate research to the forefront of global paleoclimatology with an irreplaceable role. The stalagmite paleoclimate study started in the western countries, mainly in Europe and America in 1960 s–1970 s, while the relevant research in China was progressively developed in the 1980 s–1990 s after the Reform and Opening up. Although there was a huge gap between the overall research level in China and western countries, a solid research foundation, as well as a number of talent teams were established during the period. In the 21 st century, starting from the publication of stalagmite records from Hulu Cave in Nanjing in 2001, the stalagmite paleoclimate research in China has ushered in a flourishing development and a real leap on the basis of international cooperation, resulting in significant international impacts. The landmark achievements, including establishment of the world’s longest(640000 years) East Asian monsoon stalagmite record, as well as the longest Indian monsoon(280000 years),South American monsoon(250000 years), North American westerly climate(330000 years), Central Asian westerly climate(135000 years), and northwestern China westerly climate(500000 years), have laid a milestone in the paleoclimate study in these climate domains. Importantly, these stalagmite records have revealed the relationship of Asian monsoon variations with solar

关键词： Cave stalagmites Oxygen isotope Asian monsoon Westerly Global climate change

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微冻对蚕豆籽粒保鲜效果及成熟衰老的影响

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食品科学 2025年第1期46卷 149-157页

作者：李蝶李海燕钟士宏付院生李梅青张海伟安徽农业大学茶与食品科技学院农业农村部江淮农产品精深加工与资源利用重点实验室安徽省特色农产品高值化利用工程研究中心安徽合肥230036 安徽迈涛食品有限公司安徽芜湖241000

探究微冻技术对新鲜蚕豆籽粒的保鲜效果。通过测定蚕豆籽粒的冰点,确定3个微冻温度,以冷藏(4℃)为对照组,分析不同微冻温度(-2、-3、-4℃)贮藏对蚕豆籽粒感官品质、叶绿素含量、色差值等品质指标的影响,再通过多酚氧化酶(polyphenol oxi... 详细信息

探究微冻技术对新鲜蚕豆籽粒的保鲜效果。通过测定蚕豆籽粒的冰点,确定3个微冻温度,以冷藏(4℃)为对照组,分析不同微冻温度(-2、-3、-4℃)贮藏对蚕豆籽粒感官品质、叶绿素含量、色差值等品质指标的影响,再通过多酚氧化酶(polyphenol oxidase,PPO)、过氧化物酶(peroxidase,POD)、苯丙氨酸解氨酶(phenylalamine ammonia lyase,PAL)活性、总酚含量、可溶性蛋白含量及细胞组织结构等研究微冻贮藏对蚕豆籽粒成熟衰老的影响。结果表明,4℃冷藏条件下蚕豆籽粒保鲜期为7 d,3个微冻温度贮藏均能显著提高样品的感官品质,其中-3℃使蚕豆籽粒保鲜期达35 d,能显著减缓叶绿素含量的降低和质量损失率、色差值的升高,能较好地维持蚕豆籽粒的硬度,采用主成分分析也得出-3℃为最佳微冻温度。-3℃微冻贮藏能减缓PPO、PAL活性升高,维持较高的POD活性,延缓蚕豆籽粒中淀粉和可溶性蛋白的生成、总酚的积累及变化速率,还能维持细胞组织结构、形态的相对稳定,减缓蚕豆籽粒的成熟衰老。

关键词：蚕豆籽粒微冻保鲜贮藏期品质变化成熟衰老

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Improved remote sensing image target detection based on YOLOv7

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Optoelectronics Letters 2024年第4期20卷 234-242页

作者： XU Shuanglong CHEN Zhihong zhang haiwei XUE Lifang SU Huijun School of Integrated Circuit Science and EngineeringTianjin University of Technology Tianjin 300384China

Remote sensing images are taken at high altitude from above,with complex spatial scenes of images and a large number of target *** detection of image targets on large scale remote sensing images suffers from the problem of small target size and target *** paper proposes an improved model for remote sensing image detection based on you only look once version 7(YOLOv7).First,the small-scale detection layer is added to reacquire tracking frames to improve the network’s recognition ability of small-scale targets,and then Bottleneck Transformers are fused in the backbone to make full use of the convolutional neural network(CNN)+Transformer architecture to enhance the feature extraction ability of the *** that,the convolutional block attention module(CBAM)mechanism is added in the head to improve the model’s ability of small-scale ***,the non-maximum suppressed(NMS)of YOLOv7 algorithm is changed to distance intersection over union-non maximum suppression(DIOU-NMS)to improve the detection ability of overlapping targets in the *** results show that the method in this paper can improve the detection rate of small-scale targets in remote sensing images and effectively solve the problem of high overlap and is tested on the NWPU-VHR10 and DOTA1.0 datasets,and the accuracy of the improved model is improved by 6.3%and 4.2%,respectively,compared with the standard YOLOv7 algorithm.

关键词： image network union

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Displacement measurement method based on laser self-mixing interference in the presence of speckle

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Chinese Optics Letters 2020年第5期18卷 11-15页

作者： Yan Zhao haiwei zhang School of Electrical and Electronic Engineering Tianjin University of TechnologyTianjin 300384China Tianjin Key Laboratory for Control Theory and Applications in Complicated Systems Tianjin 300384China Tianjin Key Laboratory of Film Electronic and Comm unication Device Tianjin 300384China

In order to achieve the accurate measurement of displacement, this Letter presents a self-mixing interference displacement measurement method suitable for the speckle effect. Because of the speckle effect, the amplitude of the self-mixing interference signal fluctuates greatly, which will affect the measurement accuracy of displacement. The ensemble empirical mode decomposition is used to process the interference signal, which can filter out high-frequency noise and low-frequency noise at the same time. The envelope of the self-mixing interference signal is extracted by Hilbert transform, and it is used to realize the normalization of the signal. Through a series of signal processing, the influence of speckle can be effectively reduced, and the self-mixing interference signal can be transformed into standard form. The displacement can be reconstructed by fringe counting and the interpolation method. The experimental results show that the method is successfully applied to the displacement measurement in the presence of speckle, which verifies the effectiveness and feasibility of the method.

关键词： measurement displacement laser self-mixing interference

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Optical fiber amplifiers for space-division multiplexing

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Frontiers of Optoelectronics 2012年第4期5卷 351-357页

作者： Dagong JIA haiwei zhang Zhe JI Neng BAI Guifang LI College of Precision Instrument&Opto-electronics Engineering Tianjin UniversityTianjin 300072China Key Laboratory of Opto-electronics Information Technology of the Ministry of Education Tianjin 300072China College of Optics&Photonics/CREOL&FPCE University of Central Florida4000 Central Florida BoulevardOrlandoFlorida 32816-2700USA

Recently, space-division multiplexing （SDM） techniques using multi-core fiber （MCF） and few-mode fiber （FMF） have been introduced into optical fiber communication to increase transmission capacity. Two main types of optical fiber amplifiers based on the Erbium- doped fiber （EDF） and the Raman effect have been developed to amplify signals in the MCF and FMF. In this paper, we reviewed the principles and configurations of these amplifiers.

关键词： optical fiber amplifier space-division multi-plexing (SDM) multi-core fiber (MCF) few-mode fiber(FMF)

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The Establishment and Validation of the Human U937 Cell Line as a Cellular Model to Screen Immunomodulatory Agents Regulating Cytokine Release Induced by Influenza Virus Infection

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Virologica Sinica 2019年第6期34卷 648-661页

作者： Ge Liu Si Chen Ao Hu Li zhang Wenyu Sun Jungang Chen Wei Tang haiwei zhang Chunlan Liu Chang Ke Xulin Chen State Key Laboratory of Virology Wuhan Institute ofVirologyChinese Academy of SciencesWuhan 430071China University of Chinese Academy of Sciences Beijing 100049China Wuhan Virolead Biopharmaceutical Company Wuhan 430075China

Severe influenza infections are often associated with the excessive induction of pro-inflammatory cytokines,which is also referred to as"cytokine storms".Several studies have shown that cytokine storms are directly associated with influenzainduced fatal acute lung injury and acute respiratory distress *** to the narrow administration window,current antiviral therapies are often *** efforts to use immunomodulatory agents alone or in combination with antiviral agents in the treatment of influenza in animal models have resulted in the achievement of protective effects accompanied with reduced cytokine ***,there are no immunomodulatory drugs for influenza available for clinical *** models,despite being ideal to study the anti-inflammatory responses to influenza virus infection,are very costly and ***,there is an urgent need to establish fast and economical screening methods using cellbased models to screen and develop novel immunomodulatory *** this study,we screened seven human cell lines and found that the human monocytic cell U937 supports the replication of different subtypes of influenza viruses as well as the production of the important pro-inflammatory cytokines and was selected to develop the cell-based *** U937 cell model was validated by testing a panel of known antiviral and immunomodulatory agents and screening a drug library consisting of 1280 compounds comprised mostly of FDA-approved *** demonstrated that the U937 cell model is robust and suitable for the high-throughput screening of immunomodulators and antivirals against influenza infection.

关键词： Influenza Immunomodulatory agent U937 cell CCL2 CXCL10

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Mink Circovirus Can Infect Minks, Foxes and Raccoon Dogs

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Virologica Sinica 2018年第6期33卷 561-564页

作者： Yanling Yang Yuening Cheng Nan Li Shipeng Cheng Li Guo Yucheng Zhou haiwei zhang Xinyuan zhang Linzhu Ren State Key Laboratory for Molecular Biology of Special Economic Animals Institute of Special Wild Economic Animals and PlantsChinese Academy of Agricultural SciencesChangchun 130112China Jilin Provincial Key Laboratory of Animal Embryo Engineering College of Animal SciencesJilin UniversityChangchun 130112China Key Laboratory of Jilin Province for Zoonosis Prevention and Control Military Veterinary InstituteAcademy of Military Medical SciencesChangchun 130062China

Dear Editor,Mink circovirus (MiCV), which is clustered in the genus Circovirus of the family Circoviridae, was first described in minks from farms in Dalian, China in 2013 (Lian et al.2014). The complete single-stranded circular genome of the virus is 1,753 nucleotides long and contains two major open reading frames (ORFs), designated ORF1 (Rep gene)and ORF2 (Cap gene)(Lian et al. 2014;Ge et al. 2018).

关键词： PCV Foxes and Raccoon Dogs Mink Circovirus Can Infect Minks

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