The Establishment and Validation of the Human U937 Cell Line as a Cellular Model to Screen Immunomodulatory Agents Regulating Cytokine Release Induced by Influenza Virus Infection

作     者：Ge Liu Si Chen Ao Hu Li Zhang Wenyu Sun Jungang Chen Wei Tang Haiwei Zhang Chunlan Liu Chang Ke Xulin Chen 

作者机构：State Key Laboratory of VirologyWuhan Institute ofVirologyChinese Academy of SciencesWuhan 430071China University of Chinese Academy of SciencesBeijing 100049China Wuhan Virolead Biopharmaceutical CompanyWuhan 430075China 

出 版 物：《Virologica Sinica》 (中国病毒学（英文版）)

年 卷 期：2019年第34卷第6期

页      面：648-661页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by the Important Hubei Science and Technology Innovation Plan 2015ACA062 (to Xulin Chen) the Natural Science Foundation of Hubei Province (2018CFB244, to Jungang Chen) 

主　　题：Influenza Immunomodulatory agent U937 cell CCL2 CXCL10 

摘      要：Severe influenza infections are often associated with the excessive induction of pro-inflammatory cytokines,which is also referred to ascytokine storms.Several studies have shown that cytokine storms are directly associated with influenzainduced fatal acute lung injury and acute respiratory distress *** to the narrow administration window,current antiviral therapies are often *** efforts to use immunomodulatory agents alone or in combination with antiviral agents in the treatment of influenza in animal models have resulted in the achievement of protective effects accompanied with reduced cytokine ***,there are no immunomodulatory drugs for influenza available for clinical *** models,despite being ideal to study the anti-inflammatory responses to influenza virus infection,are very costly and ***,there is an urgent need to establish fast and economical screening methods using cellbased models to screen and develop novel immunomodulatory *** this study,we screened seven human cell lines and found that the human monocytic cell U937 supports the replication of different subtypes of influenza viruses as well as the production of the important pro-inflammatory cytokines and was selected to develop the cell-based *** U937 cell model was validated by testing a panel of known antiviral and immunomodulatory agents and screening a drug library consisting of 1280 compounds comprised mostly of FDA-approved *** demonstrated that the U937 cell model is robust and suitable for the high-throughput screening of immunomodulators and antivirals against influenza infection.