检索结果-南通市图书馆

23份老挝糯玉米早代系品质性状配合力分析及育种潜力评估

在线全文

学校读者我要写书评

暂无评论

中国农业科学 2024年第15期57卷 2931-2952页

作者：朱春桃任丹丹柳政岑刘长闯刘瑞琪郑洪建胡尔良林海建李静威卢艳丽汪青军四川农业大学玉米研究所上海市农业科学院作物育种栽培研究所四川天府新区乡村振兴研究院

【目的】优异品质是鲜食糯玉米育种的首要目标性状，筛选品质性状一般配合力高的早代系和优异糯玉米组合，明确老挝糯玉米材料的育种潜力，为优质鲜食糯玉米新品种的培育奠定基础。【方法】利用Maize6H-60K芯片，获得33份糯玉米材料的... 详细信息

【目的】优异品质是鲜食糯玉米育种的首要目标性状，筛选品质性状一般配合力高的早代系和优异糯玉米组合，明确老挝糯玉米材料的育种潜力，为优质鲜食糯玉米新品种的培育奠定基础。【方法】利用Maize6H-60K芯片，获得33份糯玉米材料的高质量基因型数据用于聚类分析。结合碘染试验和糯质基因分子检测，明确老挝糯玉米早代系糯质基因的类型。通过不完全双列杂交试验，对组配的439个组合的品质等性状进行表型鉴定和配合力分析，明确老挝糯玉米早代系的育种潜力。对筛选出的优势组合开展品质性状多人品鉴试验，鉴定出最有潜力的优质糯玉米组合。【结果】利用56 626个高质量SNP进行聚类分析，结果表明，老挝材料与国内材料属于不同的分支，可以被区分开来。供试的23份老挝早代系均为糯质玉米，且其中16份属于wx-D10型，3份属于wx-D7型，另外4份为未知类型。老挝糯玉米材料品质性状的遗传力均较低，介于0.14—0.35，宜在高世代进行选择。组配的439个不完全双列杂交组合蒸煮品质的均值极显著高于对照京科糯2000和石糯2号，与渝糯7号没有显著差异。老挝糯玉米早代系F02、F22、F25和F28的所有品质性状一般配合力效应值均为正值，可用于国内糯玉米品质性状的改良。优异组合M02×F02和M22×F22在多人品鉴试验中，约86%的人评价为等级优。【结论】老挝糯玉米地方种遗传变异丰富，与国内糯玉米自交系有一定的遗传距离，可能存在新的糯质基因等位变异，在糯性和果皮厚度等品质性状上有明显的优势，可用于国内糯玉米材料品质性状的改良。

关键词：糯玉米品质种质资源配合力糯质基因

Identification and functional analysis of miRNAs in developing kernels of a viviparous mutant in maize

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

The Crop Journal 2013年第2期1卷 115-126页

作者： Haiping Ding Jian Gao Mao Luo Hua Peng haijian lin Guangsheng Yuan Yaou Shen Maojun Zhao Guangtang Pan Zhiming Zhang Institute of Maize Research Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region Ministry of AgricultureSichuan Agricultural University Drug Discovery Research Center of Luzhou Medical College Luzhou Medical College Sichuan Tourism University College of Life and Basic Science Sichuan Agricultural University

Given the important roles of miRNAs in post-transcriptional gene regulation, identification of differentially expressed miRNAs will facilitate the elucidation of molecular mechanisms underlying kernel development. In this study, we constructed a small RNA library to comprehensively represent the full complement of individual small RNAs and to characterize miRNA expression profiles in pooled ears of maize(Zea mays L.) at 10, 15,20, 22, 25 and 30 days after pollination(DAP). At least 21 miRNAs were differentially expressed. The differential expression of three of these miRNAs, i.e., miR528a, miR167a and miR160b, at each stage was verified by qRT-PCR. The results indicated that these miRNAs might be involved in kernel development. In addition, the predicted functions of target genes indicated that most of the target genes are involved in signal transduction and cell communication pathways, particularly the auxin signaling pathway. The expression of candidate germination-associated miRNAs was analyzed by hybridization to a maize genome microarray, and revealed differential expression of genes involved in plant hormone signaling pathways. This finding suggests that phytohormones play a critical role in the development of maize kernels. We found that in combination with other miRNAs, miR528a regulated a putative laccase, a Ring-H2 zinc finger protein and a MADS box-like protein, whereas miR167a and miR160b regulated multiple target genes,including ARF(auxin response factor), a member of the B3 transcription factor family. All three miRNAs are important for ear germination, development and physiology. The small RNA transcriptomes and mRNA obtained in this study will help us gain a betterunderstanding of the expression and function of small RNAs in the development of maize kernel.

关键词： Ear germination-associated miRNAs Microarray hybridization qRT-PCR Zea mays

在线全文

学校读者我要写书评

暂无评论

青贮玉米新品种临青1号配套栽培技术

中国种业 2024年第7期 166-168页

作者：梅玉芹向勇李继章兰海杨永华岩张林海建徐发声杨钢彭海山罗映兵王鹏云南省临沧市种子管理站临沧677000 四川农业大学成都611134 云南省耿马县傣族佤族自治县种植业发展服务中心耿马677500 云南省凤庆县种子管理和植保植检站凤庆675999 云南省永德县种子管理站永德677699 南充市农业科学院四川南充637000

临青1号是由临沧市种子管理站和四川农业大学以自育系XL8242为母本、自育系XL1449为父本杂交选育的青贮玉米单交种。2021-2022年在云南省开展多点区域试验和生产试验,2年区域试验每667m2平均产量(干重)1520.5kg,生产试验平均产量(干重)1... 详细信息

临青1号是由临沧市种子管理站和四川农业大学以自育系XL8242为母本、自育系XL1449为父本杂交选育的青贮玉米单交种。2021-2022年在云南省开展多点区域试验和生产试验,2年区域试验每667m2平均产量(干重)1520.5kg,生产试验平均产量(干重)1493.3kg,高抗灰斑病、大斑病、小斑病、纹枯病,抗茎腐病,中抗南方锈病、普通锈病。2023年通过云南省农作物品种审定委员会审定,审定编号:滇审玉米2023162号。阐述了临青1号的特征特性、产量表现及配套栽培技术措施,为青贮玉米的选育和推广种植提供参考。

关键词：青贮玉米临青1号栽培技术

维普期刊数据库博看期刊

Cloning and Expression of the Serine Carboxypeptidase Gene in Zea mays and Its Antifungal Activity against Rhizoctonia solani

在线全文

学校读者我要写书评

暂无评论

Journal of Life Sciences 2013年第2期7卷 123-130页

作者： Li Liu Xiujing He Zhiming Zhang Maojun Zhao Jing Wang haijian lin Ya'ou Shenl Guangtang Pan Maize Research Institute of Sichuan Agricultural University//Key Laboratory of Biology and Genetic Improvement of Maize inSouthwest Region of Chinese Ministry of Agriculture Chengdu 611130 Siehuan China College of Life and Basic Sciences Sichuan Agricultural University Ya 'an 625014 Sichuan China

The authors cloned and identified a new maize serine carboxypeptidase gene named ZmSCP from R15 inbred lines seedlings which were induced by Rhizoctonia solani AGI-IA. ZmSCP encodes a 332 amino acid protein with a predicted molecular mass of 36.5 kDa and pI of 4.75. Phylogenetic analysis revealed that ZmSCP showed closer kinship with Oryza sativa and sorghum, which belong to the same evolutionary branch. Amino acid sequence analysis revealed that there are four types of amino acids in ZmSCP, the percentages of them are 43.1%, 26.9%, 13.9% and 13.1%. The authors subsequently purified the recombinant protein which expressed in Escherichia coli BL21 and analyzed its antimicrobial activities in vitro. Results showed that the recombinant protein inhibited hyphal growth of Rhizoctonia solani. The study suggests that the expression of ZmSCP is closely related to maize sheath blight resistance caused by Rhizoctonia solani. Further, the antifungal activity showed that ZmSCP may play at role in the disease resistance response.

关键词： Maize serine carboxypeptidases banded leaf and sheath blight Rhizoctonia solani.

The genetic variation of the backcross modified lines developed from the maize line 08-641 selected by different directions

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Agricultural Sciences 2012年第7期3卷 918-922页

作者： Lujiang Li Yaoyu Xiong Wensheng Chen Yongjian Liu Hai Lan haijian lin Kecheng Yang Shibin Gao Guangtang Pan College of Agronomy Sichuan Agricultural University Chengdu China Maize Research Institute Sichuan Agricultural University/Key Laboratory of Crop Genetic Resources and Improvement Ya’an China Xinjin Rural Development Burean Chengdu China

In this study, two donaors CN962 and 8065 were used to improve the resistance to northern leaf blight of the recurrent parent inbred line 08-641 (R08). A total of 79 lines (BC2F4) were developed by a bidirectional selection based on the similarity and dissimilarity in the shape and color of seeds to *** genetic variation of these lines were analyzed by 44 pairs of SSR molecular markers, the result showed that a total of 272 alleles were detected in the improved lines and R08, 123 out of them were detected in the modified lines but discarded in R08. The modified line selected based on dissimialry in the shape and corlor of seeds to R08 have lower genetic similary between R08 than that between the lines selected based on similary in the shape and color of seeds and R08, and the genetic variation of these lines were wider. It concluded that when the backcross breeding were used to improve the maize inbred lines, multidirectional selection based on phenotypic value were contribute to create and keep genetic variation.

关键词： Backcross Breeding Select Direction Genetic Variation SSR

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

隧道衬砌台车姿态自动调整系统设计研究

铁道建筑技术 2018年第2期 45-48页

作者：林海剑中铁二十二局集团有限公司北京100043

传统的全液压自动行走模板台车在姿态调整过程中存在精度低、效率低、成本高的缺点。本文综合研究测量坐标反算里程、在台车上设置测量标志定测模板调整量、用数字缸代替传统液压油缸等技术设计了具有姿态自动调整功能的智能化衬砌台车... 详细信息

传统的全液压自动行走模板台车在姿态调整过程中存在精度低、效率低、成本高的缺点。本文综合研究测量坐标反算里程、在台车上设置测量标志定测模板调整量、用数字缸代替传统液压油缸等技术设计了具有姿态自动调整功能的智能化衬砌台车,克服了上述存在的缺点。

关键词：模板台车姿态调整数字液压油缸自动

我国铁道建筑行业建立标准必要专利机制的必要性和可行性研究

在线全文

学校读者我要写书评

暂无评论

铁道建筑技术 2016年第9期 124-127页

作者：林海剑中铁二十二局集团有限公司北京100043

本文从专利、标准、标准必要专利的定义和内涵入手,分析专利和标准的对立和统一关系,进而通过分析铁道建筑行业专利和技术标准的特点,研究在我国铁道建筑行业建立标准必要专利机制的必要性和可行性。

关键词：铁道建筑技术标准专利必要专利

地方债发行是否有助于提高银行融资流动性?——来自银行投资主承销地方债限制变动的准自然实验证据

在线全文

学校读者我要写书评

暂无评论

金融研究 2024年第4期 56-74页

作者：曾海舰林灵广西大学中国—东盟经济学院广西南宁530004 广西财经学院工商管理学院广西南宁530007

2015—2021年地方债发行量累计超过36万亿元,其中80%以上由商业银行持有。作为仅次于国债的优质担保品,地方债供给增加是否促进了银行的抵押融资交易?本文利用2018年取消地方债主承销银行20%投资比例限制为准自然实验,识别银行增持地方... 详细信息

2015—2021年地方债发行量累计超过36万亿元,其中80%以上由商业银行持有。作为仅次于国债的优质担保品,地方债供给增加是否促进了银行的抵押融资交易?本文利用2018年取消地方债主承销银行20%投资比例限制为准自然实验,识别银行增持地方债对银行参与回购交易的影响。实证检验发现:20%限制取消后,银行主承销地方债增加1元可导致同期债券回购交易增加约0.12元,此结果具有较强经济显著性和统计显著性;当银行处于流动性困难程度较高、流动性创造程度较高、利息支出-利率敏感性较强、存款-利率敏感性较大的时期,增持地方债更有助于银行进行回购融资交易以缓解流动性紧张;经济发展增速较高地区的地方债被更多用于回购融资交易;银行增持地方债并未提高其回购融资成本,但提升了其未来的风险承担水平。本文研究表明,地方债发行经由抵押担保渠道显著提升了金融机构融资流动性,在处理地方债问题时,应从财政金融协调发展的整体视野出发,充分发挥地方债的担保品功能。

关键词：地方债发行银行承销银行融资流动性准自然实验

Genetic and agronomic assessment of cob traits in corn under low and normal nitrogen management conditions

在线全文

学校读者我要写书评

暂无评论

Genetic and agronomic assessment of cob traits in corn under...