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Journal of Ocean University of China 2011年第3期10卷 234-240页

作者： mi haifeng MAI Kangsen ZHANG Wenbing WU Chenglong CAI Yinghua The Key Laboratory of Mariculture Ministry of Education China Ocean University of China Qingdao 266003 P. R. China

Soybean stachyose (SBS) and phytic acid (PA) are anti-nutritional factors (ANF) which have deleterious effects on the growth and digestibility in fish. The present research studied the effects of dietary SBS and PA on the expression of three serine protease genes in the liver of Japanese flounder (Paralichthys olivaceus). These genes are trypsinogen 1 (poTRY), elastase 1 (poEL) and chymotrypsinogen 1 (poCTRY). Eight artificial diets with graded levels of supplemented ANFs were formulated to 4 levels of SBS (0.00, 0.40, 0.80 and 1.50%), 4 levels of PA (0.00, 0.20, 0.40 and 0.80), *** flounder (initial weight 2.45 g ± 0.01 g) were fed with these diets for 10 weeks with three replications per treatment. At the end of 10 weeks, supplementation of 0.40% of dietary SBS or PA significantly increased the gene expression of poTRY and poCTRY (P0.05). These results suggested that dietary SBS and dietary PA could directly affect the serine protease genes at the transcriptional level in Japanese flounder, and these genes'expression was more sensitive to dietary PA than to SBS under the current experimental conditions.

关键词： soybean stachyose phytic acid serine proteases gene expression Paralichthys olivaceus

Unveiling the emerging role of curcumin to alleviate ochratoxin A-induced muscle toxicity in grass carp(Ctenopharyngodon idella):in vitro and in vivo studies

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Journal of Animal Science and Biotechnology 2024年第4期15卷 1638-1656页

作者： Piao Zhao Lin Feng Weidan Jiang Pei Wu Yang Liu Hongmei Ren Xiaowan Jin Lu Zhang haifeng mi Xiaoqiu Zhou Animal Nutrition Institute Sichuan Agricultural UniversityChengdu 611130SichuanChina Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province Sichuan Agricultural UniversityChengdu 611130SichuanChina Key Laboratory of Animal Disease‑Resistance Nutrition Ministry of EducationMinistry of Agriculture and Rural AffairsKey Laboratory of Sichuan ProvinceChengdu 611130SichuanChina Tongwei Co. Ltd.Healthy Aquaculture Key Laboratory of Sichuan ProvinceChengdu 610041SichuanChina

Background Ochratoxin A(OTA),a globally abundant and extremely hazardous pollutant,is a significant source of contamination in aquafeeds and is responsible for severe food *** developmental toxicity of OTA and the potential relieving strategy of natural products remain *** study screened the substance curcumin(Cur),which had the best effect in alleviating OTA inhibition of myoblast proliferation,from 96 natural products and investigated its effect and mechanism in reducing OTA myotoxicity in vivo and in *** A total of 720 healthy juvenile grass carp,with an initial average body weight of 11.06±0.05 g,were randomly assigned into 4 groups:the control group(without OTA and Cur),1.2 mg/kg OTA group,400 mg/kg Cur group,and 1.2 mg/kg OTA+400 mg/kg Cur *** treatment consisted of 3 replicates(180 fish)for 60 *** Firstly,we cultured,purified,and identified myoblasts using the tissue block culture *** preliminary screening and re-screening of 96 substances,we examined cell proliferation-related indicators such as cell viability and ultimately found that Cur had the best ***,Cur could alleviate OTA-inhibited myoblast differentiation and myofibrillar development-related proteins(Myo G and MYHC)in vivo and in vitro and improve the growth performance of grass ***,Cur could also promote the expression of OTA-inhibited protein synthesis-related proteins(S6K1 and TOR),which was related to the activation of the AKT/TOR signaling ***,Cur could downregulate the expression of OTA-enhanced protein degradation-related genes(murf1,foxo3a,and ub),which was related to the inhibition of the Fox O3a signaling *** In summary,our data demonstrated the effectiveness of Cur in alleviating OTA myotoxicity in vivo and in *** study confirms the rapidity,feasibility,and effectiveness of establishing a natural product screening method targeting myoblasts to alleviate fungal toxin toxicity.

关键词： Curcumin Grass carp Myotoxic Ochratoxin A Protein deposition

Dietary protein levels changed the hardness of muscle by acting on muscle fiber growth and the metabolism of collagen in sub-adult grass carp(Ctenopharyngodon idella)

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Journal of Animal Science and Biotechnology 2023年第2期14卷 729-744页

作者： min Dong Lu Zhang Pei Wu Lin Feng Weidan Jiang Yang Liu Shengyao Kuang Shuwei Li haifeng mi Ling Tang Xiaoqiu Zhou Animal Nutrition Institute Sichuan Agricultural UniversityChengdu 611130China Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province Sichuan Agricultural UniversityChengdu 611130China Key Laboratory of Animal Disease‑Resistance Nutrition Ministry of EducationMinistry of Agriculture and Rural AffairsKey Laboratory of Sichuan ProvinceSichuan 611130China Healthy Aquaculture Key Laboratory of Sichuan Province Tongwei Co.Ltd.Chengdu ChinaSichuan 610041China Animal Nutrition Institute Sichuan Academy of Animal ScienceSichuan Animtech Feed Co.LtdChengdu 610066China.

Background:Nutrient regulation has been proven to be an effective way to improve the flesh quality in *** a necessary nutrient for fish growth,protein accounts for the highest proportion in the fish diet and is *** our team found that the effect of protein on the muscle hardness of grass carp was probably related to an increased collagen content,the mechanism for this effect has not been deeply ***,few studies have explored the protein requirements of sub-adult grass crap(Ctenopharyngodon idella).Therefore,the effects of different dietary protein levels on the growth performance,nutritional value,muscle hardness,muscle fiber growth,collagen metabolism and related molecule expression in grass carp were ***:A total of 450 healthy grass carp(721.16±1.98 g)were selected and assigned randomly to six experimen-tal groups with three replicates each(n=25/replicate),and were fed six diets with 15.91%,19.39%,22.10%,25.59%,28.53%and 31.42%protein for 60 ***:Appropriate levels of dietary protein increased the feed intake,percentage weight gain,specific growth rate,body composition,unsaturated fatty acid content in muscle,partial free amino acid content in muscle,and muscle hardness of grass *** protein levels also increased the muscle fiber density,the frequency of new muscle fibers,the contents of collagen and IGF-1,and the enzyme activities of prolyl 4-hydroxylases and lysyloxidase,and decreased the activity of matrix *** the molecular level,the optimal dietary protein increased col-lagen type Iα1(Colα1),Colα2,PI3K,Akt,S6K1,La ribonucleoprotein domain family member 6a(LARP6a),TGF-β1,Smad2,Smad4,Smad3,tissue inhibitor of metalloproteinase-2,MyoD,Myf5,MyoG and MyHC relative mRNA *** levels of the myostatin-1 and myostatin-2 genes were downregulated,and the protein expression levels of p-Smad2,Smad2,Smad4,p-Akt,Akt,LARP6 and Smad3 were ***:The appropriate levels of dietary

关键词： Collagen degradation Collagen synthesis Grass carp Muscle fibers Muscle hardness Protein

Implications of vitamin D for flesh quality of grass carp(Ctenopharyngodon idella):antioxidant ability,nutritional value,sensory quality,and myofiber characteristics

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Journal of Animal Science and Biotechnology 2023年第6期14卷 2596-2613页

作者： Yao Zhang Chaonan Li Xiaoqiu Zhou Weidan Jiang Pei Wu Yang Liu Hongmei Ren Lu Zhang haifeng mi Jiayong Tang Ruinan Zhang Lin Feng Animal Nutrition Institute Sichuan Agricultural UniversityChengdu 611130SichuanChina. Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province Sichuan Agricultural UniversityChengdu 611130China. Key Laboratory for Animal Disease‑Resistant Nutrition of China ministry of Education Sichuan Agricultural UniversityChengdu 611130China. Healthy Aquaculture Key Laboratory of Sichuan Province Tongwei Co.Ltd.Chengdu 610041SichuanChina.

Background Muscle represents a unique and complex system with many components and comprises the major edible part of *** D is a critical nutrient for animals and is known to enhance calcium absorption and immune *** recent years,dietary vitamin D supplementation in livestock has received increased attention due to biological responses including improving shear force in mammalian ***,the vitamin D acquisition and myofiber development processes in fish differ from those in mammals,and the effect of vitamin D on fish flesh quality is poorly ***,the influence of dietary vitamin D on fillet quality,antioxidant ability,and myofiber development was examined in grass carp(Ctenopharyngodon idella).Methods A total of 540 healthy grass carp,with an initial average body weight of 257.24±0.63 g,were allotted in 6 experimental groups with 3 replicates each,and respectively fed corresponding diets with 15.2,364.3,782.5,1,167.9,1,573.8,and 1,980.1 IU/kg vitamin D for 70 *** Supplementation with 1,167.9 IU/kg vitamin D significantly improved nutritional value and sensory quality of fillets,enhancing crude protein,free amino acid,lipid,and collagen contents;maintaining an ideal pH;and reduc-ing lactate content,shear force,and cooking loss relative to respective values in the control(15.2 IU/kg)*** myofiber diameter and the frequency of myofibers>50μm in diameter increased under supplementation with 782.5–1,167.9 IU/kg vitamin *** of oxidative damage biomarkers decreased,and the expression of antioxi-dant enzymes and nuclear factor erythroid 2-related factor 2 signaling molecules was upregulated in the 1,167.9 IU/kg vitamin D treatment compared to respective values in the control ***,vitamin D supplementation activated cell differentiation by enhancing the expression of myogenic regulatory factors and myocyte enhancer factors compared to that in the control *** addition,supplementation with 1,167.9 IU/kg

关键词： Antioxidant Grass carp Myofiber development Nutritional value Sensory quality Vitamin D

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浅谈优化矿井通风与安全生产的关系

能源与节能 2015年第1期 54-55页

作者：米海峰霍州煤电集团公司三交河煤矿安全培训科山西霍州031400

矿井通风是煤矿生产的重要环节。全国每年的煤矿安全事故有数十起,其中大部分都是由于通风设施不完善引起的。介绍了矿井通风的意义与重要性,分析了目前矿井通风方面存在的主要问题,并对此提出了几点建议。

关键词：矿井通风安全生产优化

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矿用通风机运行状态监控系统的研究

机械管理开发 2022年第7期37卷 303-304页

作者：米海峰霍州煤电集团三交河煤矿山西临汾041600

针对矿井通风机运行故障监测可靠性不足,影响矿井通风安全的情况,提出了一种新的基于振动传感器的矿用通风机运行状态监控系统。通过对风机运行常见隐秘故障特性的分析,获取其振动特性值,利用振动传感器对风机运行时的振动特性进行监测... 详细信息

针对矿井通风机运行故障监测可靠性不足,影响矿井通风安全的情况,提出了一种新的基于振动传感器的矿用通风机运行状态监控系统。通过对风机运行常见隐秘故障特性的分析,获取其振动特性值,利用振动传感器对风机运行时的振动特性进行监测,经过小波神经网络智能分析后确定风机的运行特性,实现对隐秘故障的快速识别和定位。根据实际应用表明,该监测系统对风机隐秘故障的检测精度达88.5%以上,实现了对隐秘故障的精确监测。

关键词：振动传感器通风机小波神经网络

表观弥散系数直方图对中耳胆脂瘤与慢性化脓性中耳炎的鉴别诊断价值

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中华解剖与临床杂志 2020年第6期25卷 601-605页

作者：米海峰李铮鲜军舫首都医科大学附属北京同仁医院放射科北京100730

目的探讨基于表观弥散系数(ADC)值的直方图分析方法在鉴别中耳胆脂瘤和慢性化脓性中耳炎中的最佳指标与诊断效能。方法回顾性分析。纳入2017年1月—2019年12月首都医科大学附属北京同仁医院放射科经手术病理证实的64例中耳胆脂瘤(胆脂瘤... 详细信息

目的探讨基于表观弥散系数(ADC)值的直方图分析方法在鉴别中耳胆脂瘤和慢性化脓性中耳炎中的最佳指标与诊断效能。方法回顾性分析。纳入2017年1月—2019年12月首都医科大学附属北京同仁医院放射科经手术病理证实的64例中耳胆脂瘤(胆脂瘤组)及56例慢性化脓性中耳炎(中耳炎组)患者MRI资料。采用ImageJ软件在ADC图病变显示的最大层面勾画感兴趣区(ROI),通过SPSS统计软件对直方图分析获得ADC值的均数、标准差、偏度、峰度、第10百分位数(P10)和第90百分位数(P90)等指标进行统计学分析,比较两组直方图各参数指标的差异,并绘制有差异的ADC值指标鉴别两种病变的受试者操作特征曲线(ROC),获得各指标的ROC下面积(AUC)值。采用logistic回归分析方法探讨鉴别两种病变的最佳独立诊断指标。结果胆脂瘤组和中耳炎组间ADC值的均数、标准差、偏度、P10和P90差异均有统计学意义(P值均<0.01),且鉴别两种病变的AUC值分别为0.912、0.988、0.809、0.793和0.957,其中ADC值标准差的AUC值最大,灵敏度和特异度分别是95.31%和96.43%。Logistic回归分析结果显示,ADC值的标准差为鉴别中耳胆脂瘤和慢性化脓性中耳炎的最佳独立诊断指标(OR=1.034,95%可信区间1.020~1.048,P<0.01)。结论基于ADC值的直方图分析方法有助于提高鉴别中耳胆脂瘤和慢性化脓性中耳炎的能力,ADC值标准差的鉴别效能最佳。

关键词：胆脂瘤中耳中耳炎化脓性磁共振成像弥散加权成像表观弥散系数诊断鉴别直方图

低氧胁迫对鱼类的影响以及营养调控策略的研究进展

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动物营养学报 2023年第10期35卷 6207-6219页

作者：张雷敏梁化亮米海峰任鸣春南京农业大学无锡渔业学院无锡214081 中国水产科学研究院淡水渔业研究中心农业农村部淡水渔业和种质资源利用重点实验室无锡214081 通威农业发展有限公司成都610041

氧气对需氧生物的生活和生产至关重要,水体溶解氧(DO)更是鱼类获得氧气的主要来源和生存的重要前提。低氧是指水体环境中的DO含量低于2 mg/L的现象,在水产养殖中,低氧会对鱼类的生长、代谢和行为活动等产生不利影响,进而影响养殖效果。... 详细信息

氧气对需氧生物的生活和生产至关重要,水体溶解氧(DO)更是鱼类获得氧气的主要来源和生存的重要前提。低氧是指水体环境中的DO含量低于2 mg/L的现象,在水产养殖中,低氧会对鱼类的生长、代谢和行为活动等产生不利影响,进而影响养殖效果。鱼类能够适应一定范围内的低氧状态,但是长期和更深程度的低氧会严重危害鱼类甚至导致死亡,因此还需要采取一定的外部措施来降低或者消除低氧的危害。本文分别对低氧胁迫对鱼类生长、行为和生化指标的影响、相关的基因表达过程等方面的研究进展进行了概述,总结了目前已有的一些应对水产养殖低氧的相关方案,并重点整理陈述了营养调控策略的应用,以期为鱼类逆境相关研究以及水产健康养殖提供参考。

关键词：低氧机理鱼类营养调控添加剂

不同来源菜籽粕对鲤鱼生长、消化生理、抗氧化能力和健康的影响

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动物营养学报 2023年第1期35卷 469-493页

作者：袁梦莹王恒志米海峰张璐邓君明广东海洋大学水产学院湛江524088 云南农业大学动物科学技术学院昆明650201 通威股份有限公司成都610041

本试验旨在探究鲤鱼对5种菜籽粕[加拿大菜籽粕(CCM)、印度菜籽粕(ICM)、冷生榨菜籽饼(CPCC)、国产95型菜籽粕(C95CM)和国产200型菜籽粕(C200CM)]利用效率的差异,以期为菜籽粕在鲤鱼饲料中的应用提供理论依据。以豆粕为主要蛋白质源配制... 详细信息

本试验旨在探究鲤鱼对5种菜籽粕[加拿大菜籽粕(CCM)、印度菜籽粕(ICM)、冷生榨菜籽饼(CPCC)、国产95型菜籽粕(C95CM)和国产200型菜籽粕(C200CM)]利用效率的差异,以期为菜籽粕在鲤鱼饲料中的应用提供理论依据。以豆粕为主要蛋白质源配制基础饲料(含46%豆粕),于基础饲料(对照组)中分别添加20%(CCM-Ⅰ组、ICM-Ⅰ组、CPCC-Ⅰ组、C95CM-Ⅰ组和C200CM-Ⅰ组)或35%(CCM-Ⅱ组、 ICM-Ⅱ组、 CPCC-Ⅱ组、 C95CM-Ⅱ组和C200CM-Ⅱ组)5种不同来源菜籽粕等蛋白质替代豆粕共配制11种等氮等能饲料。取990尾健壮均匀的幼鲤,随机分为11个组,每组3个网箱,每个网箱30尾,饲喂8周。结果表明:1)CPCC-Ⅰ组、CPCC-Ⅱ组、ICM-Ⅰ组和ICM-Ⅱ组饲料中硫代葡萄糖苷(GLS)、异硫氰酸酯(ITC)和噁唑烷硫酮(OZT)含量分别为1.31%、 0.26 g/kg和0.62 mg/g, 2.30%、 0.45 g/kg和1.08 mg/g,0.12%、0.37 g/kg和0.01 mg/g,0.21%、0.65 g/kg和0.02 mg/g;其中CPCC组GLS、OZT含量和ICM组ITC含量分别高于其余4组,且ICM组GLS和OZT含量极低。2)在20%替代水平下,CPCC-Ⅰ组和ICM-Ⅰ组日增重系数(DGC)显著低于对照组(P0.05);ICM-Ⅰ组DGC显著低于C200CM-Ⅰ组(P0.05);CPCC-Ⅰ组饲料系数(FCR)显著高于对照组、C200CM-Ⅰ组、ICM-Ⅰ组和CCM-Ⅰ组(P0.05)。但在35%替代水平下,ICM-Ⅱ组和CPCC-Ⅱ组DGC显著低于对照组、C95CM-Ⅱ组、C200CM-Ⅱ组和CCM-Ⅱ组(P0.05);C95CM-Ⅱ组、CPCC-Ⅱ组和ICM-Ⅱ组FCR显著高于对照组(P0.05)。3)在20%和35%替代水平下,各菜籽粕组肠道胰蛋白酶和脂肪酶活性有所下降,且CPCC组和ICM组胰蛋白酶活性(尤其是ICM组)和CPCC组脂肪酶活性下降尤为显著(P<0.05)。同时,肠道组织苏木精-伊红(HE)染色和电镜扫描切片显示,各菜籽粕组杯状细胞均增多,尤其是ICM-Ⅱ组微绒毛暴露出大量脂滴。4)在20%替代水平下,C95CM-Ⅰ组和ICM-Ⅰ组血清过氧化氢酶(CAT)活性显著低于对照组(P <0.05),且CPCC-Ⅰ组肝脏CAT活性显著低于对照组、C95CM-Ⅰ组、C200CM-Ⅰ组和CCM-Ⅰ组(P<0.05)。在35%替代水平下,除C200CM-Ⅱ以外,各菜籽粕组血清CAT活性以及CPCC-Ⅱ组、ICM-Ⅱ组肝脏CAT活性和血清三碘甲状腺原氨酸(T3)含量显著低于对照组(P<0.05);CPCC-Ⅱ组、ICM-Ⅱ组和CCM-Ⅱ组血清碱性磷酸酶(AKP)活性显著高于对照组(P <0.05)。此外,肝脏组织HE染色切片显示,C200CM-Ⅱ组、CPCC-Ⅱ组和ICM-Ⅱ组出现脂肪肝症状;同时,电镜扫描切片结果显示,ICM-Ⅱ组肝脏被膜被破坏,漏出大量脂滴和线粒体;且各菜籽粕组均出现甲状腺滤泡结构不规则、滤泡上皮细胞高度增加等现象。5)在20%替代水平下,ICM-Ⅰ组血清免疫球蛋白M(IgM)、补体4(C4)含量和CPCC-Ⅰ组血清C4含量显著低于对照组(P<0.05),且2组攻毒成活率最低(均为46.7%)。在35%替代水平下,C95CM-Ⅱ组和ICM-Ⅱ组血清IgM含量显著低于对照组、C200CM-Ⅱ组和CCM-Ⅱ组(P<0.05);且ICM-Ⅱ组血清C4含量显著低于对照组、C95CM-Ⅱ和C200CM-Ⅱ组(P<0.05),CPCC-Ⅱ组显著低于对照组(P<0.05),同时ICM-Ⅱ组攻毒成活率最低(36.7%)。6)菜籽粕添加水平提高后,对鲤鱼生长、消化和健康负面影响加重,其中ICM组DGC显著下降(P<0.05),FCR显著升高(P<0.05)。综上所述:1)菜籽粕饲料中的ITC、GLS和OZT是危害鲤鱼生长、消化和健康的关键因素,尤其是ITC。饲料中含0.37 mg/g的ITC即可导致鲤鱼生长速度减慢,但对FCR无显著负面影响;然而,当饲料中含0.65 mg/g的ITC时,会对鲤鱼的生长、消化能力、抗氧化能力和健康产生严重负面

关键词：鲤鱼菜籽粕生长消化生理异硫氰酸酯抗氧化能力免疫力

饲料中脂肪水平对团头鲂幼鱼生长性能、血清生化指标及黏蛋白基因表达的影响

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动物营养学报 2018年第9期30卷 3559-3566页

作者：习丙文陆春云任鸣春米海峰谢骏农业部淡水渔业与种质资源利用重点实验室中国水产科学研究院淡水渔业研究中心无锡214081 南京农业大学无锡渔业学院无锡214081 通威股份有限公司成都610000

本试验旨在研究饲料中不同脂肪水平对团头鲂幼鱼生长性能、血清生化指标及黏蛋白基因表达的影响。试验选取540尾初始体重为(10.0±0.5)g的团头鲂幼鱼,随机分为6组,每组3个重复,每个重复30尾鱼,分别投喂脂肪水平为2.29%、4.29%、6.29%、8... 详细信息

本试验旨在研究饲料中不同脂肪水平对团头鲂幼鱼生长性能、血清生化指标及黏蛋白基因表达的影响。试验选取540尾初始体重为(10.0±0.5)g的团头鲂幼鱼,随机分为6组,每组3个重复,每个重复30尾鱼,分别投喂脂肪水平为2.29%、4.29%、6.29%、8.29%、10.29%和12.29%的等氮等能试验饲料。饲养8周后检测鱼体生长指标、血清生化指标和表皮、肠道中黏蛋白基因的表达。结果表明:1)6.29%和8.29%组的增重率(WGR)显著高于其他组(P0.05)。3)8.29%组鱼体肝脏中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性和还原型谷胱甘肽(GSH)含量显著高于2.29%组(P0.05),但显著高于其他各组(P<0.05)。5)病原菌感染试验表明,2.29%和12.29%组死亡率较高,6.29%和8.29%组死亡率较低,且显著低于其他各组(P<0.05)。综上,在本试验条件下,饲料脂肪水平在6.29%~8.29%时,团头鲂幼鱼生长性能、抗病力和表皮黏蛋白Muc5b基因表达量最高。

关键词：团头鲂脂肪黏蛋白 Muc5b