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Pollution and biodegradation of hexabromocyclododecanes:A review

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Frontiers of Environmental Science & Engineering 2020年第1期14卷 35-42页

作者： Ling huang Syed Bilal Shah haiyang hu Ping Xu Hongzhi Tang State Key Laboratory of Microbial Metabolism Joint International Research Laboratory of Metabolic and Developmental SciencesSchool of Life Sciences and BiotechnologyShanghai Jiao Tong UniversityShanghai 200240China

Hexabromocyclododecanes(HBCDs)are the most common brominated flame-retardants after polybrominated diphenyl *** can induce cancer by causing inappropriate antidiuretic hormone *** contamination with HBCDs has been detected globally,with concentrations ranging from ng to|*** to degrade HBCDs include physicochemical methods,bioremediation,and *** photodegradation of HBCDs using simulated sunlight or ultraviolet lamps,or chemical catalysts are inefficient and expensive,as is physicochemical ***,bioremediation is considered as the most cost-effective and clean *** date,five bacterial strains capable of degrading HBCDs have been isolated and identified:Pseudomonas ***01,Bacillus ***-sjtu,Achromobacter ***-1,Achromobacter ***-2,and Pseudomonas aeruginosa *** molecular mechanisms of biodegradation of HBCDs are discussed in this *** microbial resources should be explored to increase the resource library in order to identify more HBCD-degrading microbes and functional *** biology methods may be exploited to accelerate the biodegradation capability of existing bacteria,including modification of the degrading strains or functional enzymes,and artificial construction of the degradation *** most potentially useful method is combining microdegradation with physicochemical methods and *** example,exogenous microorganisms might be used to stimulate the adsorption capability of plants for HBCDs,or to utilize an interaction between exogenous microorganisms and rhizosphere microorganisms to form a new rhizosphere microbial community to enhance the biodegradation and absorption of HBCDs.

关键词： Hexabromocyclododecane Biodegradation Bioremediation Phytoremediation Bacterium

Boring holes in Au nanoplates by active surface etching

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Nano Research 2024年第9期17卷 8610-8617页

作者： haiyang hu Yuntao Wang Qian Wang Xudong Peng An Su Hong Wang Hongyu Chen Institute of Advanced Synthesis(IAS)and School of Chemistry and Molecular Engineering Jiangsu National Synergetic Innovation Centre for Advanced MaterialsNanjing Tech UniversityNanjing 211816China Department of Environmental Science and Engineering University of Science and Technology of ChinaHefei 230026China Department of Chemistry School of Science and Key Laboratory for Quantum Materials of Zhejiang ProvinceResearch Center for Industries of the FutureWestlake UniversityHangzhou 310030China Institute of Natural Sciences Westlake Institute for Advanced StudyHangzhou 310024China

In contrast to the conventional etching that makes nanoparticles rounder and our previous sharpening etching mode that causes serrated edges,here,we developed a new boring etching mode that targets the faces of Au nanoplates to make *** critical factors are the pre-incubation step with the ligand 2-mercapto-5-benzimidazolecarboxylic acid(MBIA)and the subsequent removal of excess ligands in the ***,etching is focused onto the few sites with initial loss of ligands,which cannot be quickly *** choice of ligand MBIA is also of importance,as it carries negative charge and repels each *** inability of forming a dense layer probably plays a critical role in the site-selectivity for faces,because ligands at the higher curvature edges and corners are expected to have less *** etching results from the comproportionation reaction between Au3+and Au0 in the nanoplates,where Br-coordination to Au and the extra stabilization from cetyltrimethylammonium bromide(CTAB)are *** believe that the ability of boring holes is an important tool for future synthetic designs.

关键词： Au nanoplates active surface etching boring etching mode cetyltrimethylammonium bromide(CTAB)passivation holes

Microbial production of cis,cis-muconic acid from aromatic compounds in engineered Pseudomonas

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Synthetic and Systems Biotechnology 2023年第3期8卷 536-545页

作者： Siyang He Weiwei Wang Weidong Wang haiyang hu Ping Xu Hongzhi Tang State Key Laboratory of Microbial Metabolism and School of Life Sciences&BiotechnologyShanghai Jiao Tong UniversityShanghai200240People’s Republic of China College of Life Science Northeast Forestry UniversityHarbin150040China

Industrial expansion has led to environmental pollution by xenobiotic compounds like polycyclic aromatic hydrocarbons and monoaromatic *** *** broad metabolic potential for degrading aromatic *** objective of this study was to develop a“biological funneling”strategy based on genetic modification to convert complex aromatic compounds into cis,cis-muconate(ccMA)using Pseudomonas putida B6-2 and *** MPDS as *** engineered strains B6-2(B6-2ΔcatBΔsalC)and MPDS(MPDSΔsalC(pUCP18k-catA))thrived with biphenyl or naphthalene as the sole carbon source and produced ccMA,attaining molar conversions of 95.3%(ccMA/biphenyl)and 100%(ccMA/naphthalene).Under mixed substrates,B6-2ΔcatBΔsalC grew on biphenyl as a carbon source and transformed ccMA from non-growth substrates benzoate or salicylate to obtain higher product *** exogenous clusters like bedDC1C2AB and xylCMAB allowed B6-2 recombinant strains to convert benzene and toluene to *** mixed substrates,constructed consortia of engineered strains B6-2 and MPDS specialized in catabolism of biphenyl and naphthalene;the highest molar conversion rate of ccMA from mixed substrates was 85.2%when B6-2ΔcatBΔsalC was added after 24 h of MPDSΔsalC(pUCP18k-catA)incubation with biphenyl and *** study provides worthwhile insights into efficient production of ccMA from aromatic hydrocarbons by reusing complex pollutants.

关键词： Biodegradation Polycyclic aromatic hydrocarbons Biological funneling cis,cis-muconic acid Pseudomonas

Calculation of Wall Temperature for Aircraft Exhaust System with Considering Gas Radiation Heat Transfer

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Chinese Journal of Aeronautics 2009年第6期22卷 590-598页

作者： hu haiyang Wang Qiang School of Propulsion Beijing University of Aeronautics and Astronautics Beijing 100191 China

The coupled numerical simulation of flow field, solid temperature field, species concentration field and gas radiation transfer/ energy field based on statistical narrow-band correlated-k （SNBCK） model, is employed to accurately predict aerothermodynamic characteristic of aircraft exhaust system. A series of methods to increase computational efficiency and descend computational resources make it possible to finish the calculation in PC. The parameters of narrow-band model are evaluated by HITEMP line-by-line database. Three examples have proved the accuracy of using these methods to solve flow heat transfer coupled problem and radiation transfer/energy equation, which are the calculation of temperature distribution of water-cooling nozzle in rocket engine, the calculation of carbon dioxide absorptivity at 4.3 micron band, and the gas radiation heat transfer evaluation of the cylindrical furnace. Finally, the inner flaps temperature distribution of ejecting nozzle with floating outer flaps is computed, under high-altitude, high-speed and afterburning conditions. Two completely different air-inlet schemes of ejecting channel almost achieve the same effect in cooling inner flaps.

关键词： numerical simulation gas radiation exhausting nozzles coupled model statistical narrow-band correlated-k model

Preparation and evaluation of poly(L-histidine) based pH-sensitive micelles for intracellular delivery of doxorubicin against MCF-7/ADR cells

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Asian Journal of Pharmaceutical Sciences 2017年第5期12卷 433-441页

作者： Nan Jia Yuqing Ye Qi Wang Xiuli Zhao haiyang hu Dawei Chen Mingxi Qiao Department of Pharmaceutics School of Pharmacy Shenyang Pharmaceutical University

In this study, a p H-sensitive micelle self-assembled from poly(L-histidine) based triblock copolymers of poly(ethylene glycol)–poly(D,L-lactide)–poly(L-histidine)(mPEG-PLA-PHis) was prepared and used as the intracellular doxorubicin(Dox) delivery for cancer chemotherapy. Dox was loaded into the micelles by thin-film hydration method and a Box–Behnken design for three factors at three levels was used to optimize the preparations. The optimized mPEG-PLA-Phis/Dox micelles exhibited good encapsulation efficiency of 91.12%,a mean diameter of 45 nm and narrow size distribution with polydispersity index of *** vitro drug release studies demonstrated that Dox was released from the micelles in a p Hdependent manner. Furthermore, the cellular evaluation of Dox loaded micelles displayed that the micelles possessed high antitumor activity in vitro with an IC50 of 35.30 μg/ml against MCF-7/ADR cells. The confocal microscopy and flow cytometry experiments indicated that m PEG-PLA-Phis micelles mediated efficient cytoplasmic delivery of Dox with the aid of poly(Lhistidine) mediated endosomal escape. In addition, blank m PEG-PLA-Phis micelles were shown to be nontoxic to MCF-7/ADR cells even at a high concentration of 200 μg/ml. The pHsensitive mPEG-PLA-PHis micelles have been demonstrated to be a promising nanosystem for the intracellular delivery of Dox for MDR reversal.

关键词： Doxorubicin(Dox) mPEG-PLA-Phis pH sensitive micelles Box–Behnken design

Assessment of POC CD4 Detecting Mode in District or County Labs-Jiangsu Province, China, 2021

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China CDC weekly 2022年第47期4卷 1059-1065,I0003页

作者： Zhi Zhang Xiaoqin Xu Chengqing Sun Jing Lu haiyang hu Ying Zhou Gengfeng Fu Department of HIV/STD Control and Prevention Jiangsu Provincial Center for Disease Control and PreventionNanjing CityJiangsu ProvinceChina School of Public Health Nanjing Medical UniversityNanjing CityJiangsu ProvinceChina.

Objective:This study seeks to explore efficient and multiple-item detection modes in new-style HIV labs,as well as access the accuracy and reliability of CD4 cell count detected by point of care(POC)to analyze POC work feasibility in district or county ***:POC devices adopted in grassroots-level labs and flow cytometers adopted in prefecture-level labs were used to analyze the same group of blood *** individual results were collected and compared for parametric tests in correlation and ***:The Pearson correlation coefficients(r)between results detected by FACSPresto and those by FACSCalibur,FACSVia,FACSCantoII,and EPICSXL were 0.922,0.938,0.914,and 0.823,respectively;the average deviations were−25.64,24.68,3.05,and 70.97 cells/μL,respectively;the Pearson correlation coefficient(r)between results by Pima and FACSCalibur,FACSVia,FACSCantoII,and EPICSXL were 0.900,0.950,0.954,and 0.876,respectively;and the average deviations were−73.99,−40.78,−29.32,and−22.75 cells/μL,***:Strong positive correlations and good consistency were observed between the CD4 count tested by POC and flow *** findings provide theoretical support for new-style HIV labs and one-stop services,which can provide shorter testing duration and simpler testing processes,so that the most comprehensive testing results can be obtained in the shortest amount of time.

关键词： testing simpler shortest

Preparation of CaP/pDNA nanoparticles by reverse micro-emulsion method: Optimization of formulation variables using experimental design

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Asian Journal of Pharmaceutical Sciences 2017年第2期12卷 179-186页

作者： Wenpan Li Shasha Jing Xiu Xin Xirui Zhang Kang Chen Dawei Chen haiyang hu Department of Pharmaceutics School of Pharmacy Shenyang Pharmaceutical University

In this study, the CaP/pDNA nanoparticles were prepared using Triton X-100/Butanol/Cyclohexane/Water reverse microemulsion system. Optimization of preparation conditions was based on evaluation of particle size by Box–Behnken design method. The particle sizes of the optimized CaP/pDNA nanoparticles were found to be 60.23 ± 4.72 nm, polydispersity index was 0.252 and pDNA encapsulate efficiency was more than 90%. The optimized CaP/pDNA nanoparticles have pH sensitivity and biocompatibility. Further, optimized CaP/pDNA nanoparticles showed higher transfection efficiency.

关键词： CaP nanoparticles pDNA Reverse microemulsion method Box–Behnken design Transfection and expression

Effect of surface ligand density on cytotoxicity and pharmacokinetic profile of docetaxel loaded liposomes

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Asian Journal of Pharmaceutical Sciences 2016年第5期11卷 655-661页

作者： Chun Chu Ping Xu Haoyue Zhao Qing Chen Dawei Chen haiyang hu Xiuli Zhao Mingxi Qiao Shenyang Pharmaceutical University

Various biotin-modified liposomes incorporated with docetaxel(DTX) were prepared to study the effect of surface biotin density on the pharmacokinetic profile of the liposome. Four types of liposomes such as PEG modified liposome(PDL), 0.5%(mol) biotin modified liposome(0.5 BDL), 1%(mol) biotin modified liposome(1 BDL) and 2%(mol) biotin modified liposome(2 BDL) were prepared using thin film dispersion method. The prepared liposomes were characterized by measuring encapsulation efficiency(EE), particle size, Zeta-potential, physical stability and drug release profiles in vitro. MTT assay was performed to elevate the cytotoxicity of liposomes on MCF-7 cells. In vivo evaluation was further performed to investigate the effect of biotin surface density on the pharmacokinetic profiles. All the prepared liposomes exhibited high encapsulation efficiency, small particle size, narrow particle distribution and sustained release profiles in vitro. In MTT assay, 0.5 BDL showed largest tumor cell toxicity, compared with DTX solution. All liposomes containing DTX showed prolonged blood circulation in vivo, and 0.5 BDL showed the longest circulation time among the biotin modified liposome. Surface modification of liposome had a negative impact on the circulation of liposomes in the blood, which needs to be considered when designing the ligand mediated targeting delivery systems. A proper amount of biotin liposome with 0.5% molar ratio is expected to produce the best anti-tumor effect.

关键词： Docetaxel Liposomes Biotin Ligand density Tumor targeting

Combining immune checkpoint blockade with ATP-based immunogenic cell death amplifier for cancer chemo-immunotherapy

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Acta Pharmaceutica Sinica B 2022年第9期12卷 3694-3709页

作者： Jiulong Zhang Xiaoyan Sun Xiufeng Zhao Chunrong Yang Menghao Shi Benzhuo Zhang haiyang hu Mingxi Qiao Dawei Chen Xiuli Zhao School of Pharmacy Shenyang Pharmaceutical UniversityShenyang 110016China Department of Oncology Affiliated Hongqi Hospital of Mudanjiang Medical CollegeMudanjiang 157011China Department of Pharmacy Shantou University Medical CollegeShantou 515041China Department of Neurology The Second Affiliated Hospital of Mudanjiang Medical CollegeMudanjiang 157009China

Amplifying“eat me signal”during tumor immunogenic cell death(ICD)cascade is crucial for tumor *** by the indispensable role of adenosine triphosphate(ATP,a necessary“eat me signal”for ICD),a versatile ICD amplifier was developed for chemotherapy-sensitized ***(DOX),ATP and ferrous ions(Fe^(2+))were co-assembled into nanosized amplifier(ADO-Fe)throughπ‒πstacking and coordination ***,phenylboric acid-polyethylene glycol-phenylboric acid(PBA-PEG-PBA)was modified on the surface of ADO-Fe(denoted as PADO-Fe)by the virtue of d-ribose unit of ***-Fe could display active targetability against tumor cells via sialic acid/PBA *** acidic microenvironment,PBA-PEG-PBA would dissociate from ***,high H_(2)O_(2)concentration would induce hydroxyl radical(·OH)and oxygen(O_(2))generation through Fenton reaction by Fe^(2+).DOX and ATP would be released from the amplifier,which could induce ICD effect and“ICD adjuvant”to amplify this *** with programmed death ligands 1(PD-L1)checkpoint blockade immunotherapy,PADO-Fe could not only activate immune response against primary tumor,but also strong abscopal effect against distant *** simple and multifunctional ICD amplifier opens a new window for enhancing ICD effect and immune checkpoint blockade therapy.

关键词： Immunogenic cell death amplifier ATP Fenton reaction Immune checkpoint blockade Coordination polymer nanoparticles pH/ROS sensitive Phenylboric acid