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World Journal of Gastroenterology 2007年第41期13卷 5440-5445页

作者： Fen-Yu Ren hai Jin Xi-Xu piao Feng-Shun piao Department of Gastroenterology and Hepatology Yanbian University Hospital Yanji 133000 Jilin Province China

AIM: To investigate the anti-viral mechanism of combination therapy of interferon (IFN)-α and ribavirin in patients with chronic hepatitis B. METHODS: Twenty patients were assigned to receive either IFN-α plus ribavirin (group A,n = 14) or no treatment as a control (group B,n = 6). Patients were analyzed for T-cell proliferative responses specific for hepatitis B virus (HBV)-antigen and cytokine production by peripheral blood mononuclear cells (PBMCs). RESULTS: Combination therapy induced HBV-antigen specific CD4+ T-cell proliferative responses in four patients (28.6%). Production of high levels of HBV-specific IFN-γ,tumor necrosis factor (TNF)-α,interleukin (IL)-12 by PBMCs was found in five patients (35.7%),who showed significantly lower HBV DNA levels in serum at 12 mo after treatment ended (P = 0.038) and at 24 mo of follow-up (P = 0.004) than those without high levels of cytokine production. CONCLUSION: HBV-antigen specific CD4+ T cells may directly control HBV replication and secretion of anti-viral T helper 1 (Th1) cytokines by PBMCs during combination therapy of chronic hepatitis B with ribavirin and IFN-α.

关键词： Hepatitis B Interferon-alpha Ribavirin CD4+ T cells Th1

2022年全国生物剂量估算和染色体畸变分析能力比对结果分析

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中华放射医学与防护杂志 2024年第3期44卷 223-227页

作者：潘艳阮健磊高刚朴春南刘建香中国疾病预防控制中心辐射防护与核安全医学所辐射防护与核应急中国疾病预防控制中心重点实验室北京100088

目的为了提高放射卫生技术机构生物剂量估算能力和水平,促进放射工作人员职业健康检查机构的染色体畸变分析能力提升。方法组织全国范围内的疾控中心、职防院(所)、大专院校、科研院所、核工业系统、医疗及体检机构等共144家机构开展202... 详细信息

目的为了提高放射卫生技术机构生物剂量估算能力和水平,促进放射工作人员职业健康检查机构的染色体畸变分析能力提升。方法组织全国范围内的疾控中心、职防院(所)、大专院校、科研院所、核工业系统、医疗及体检机构等共144家机构开展2022年全国生物剂量估算能力比对工作。参加比对机构分为A类和B类两类比对,通过对染色体畸变的识别,来估算照射剂量(A类)或染色体畸变率(B类),组织机构对比对结果进行统计分析和汇总,并对不合格的原因和存在的主要问题进行分析和探讨。结果参加本次生物剂量估算比对A类的机构有60家,其中合格的52家(优秀12家),不合格的8家,合格率为86.7%(其中优秀比例为20.0%),不合格率为13.3%。参加本次生物剂量估算比对B类的机构有84家,合格的机构有48家,不合格的机构有36家,合格率为57.1%,不合格率为42.9%。结论大部分参加A类比对的机构具备生物剂量估算的能力,参加B类比对的机构,一半以上具有分析染色体畸变的能力,参加A类比对的机构总体能力高于参加B类的机构;通过该项工作,提高了全国相关机构在生物剂量估算和染色体畸变分析方面的能力。

关键词：放射卫生生物剂量估算染色体畸变分析比对

人参皂苷Rb1通过AMPK/SIRT1/PGC-1α轴调节线粒体裂变融合缓解哮喘气道炎症

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中国药理学通报 2024年第4期40卷 798-799页

作者：韩雪白巧云申林卜佳亮吴枧李卓俊延光海宋艺兰朴红梅吉林省过敏性常见疾病免疫与靶向研究重点实验室吉林延吉133000 延边大学附属医院呼吸内科吉林延吉133000 延边大学医学院解剖学教研室吉林延吉133000 延边大学医学院吉林延吉133000

哮喘(bronchial asthma)以气道炎症和高反应性为主要特征,严重危害人类健康^([1])。AMP-activated protein kinase(AMPK)作为氧化还原蛋白,能有效调节细胞内氧化应激,可通过激活高度保守的NAD+依赖性去乙酰化酶Sirtuin 1(SIRT1)/NF-κB... 详细信息

哮喘(bronchial asthma)以气道炎症和高反应性为主要特征,严重危害人类健康^([1])。AMP-activated protein kinase(AMPK)作为氧化还原蛋白,能有效调节细胞内氧化应激,可通过激活高度保守的NAD+依赖性去乙酰化酶Sirtuin 1(SIRT1)/NF-κB通路抑制哮喘^([2])。PPARγcoactivator-1α(PGC-1α)在线粒体生物合成和功能调节中得到广泛应用^([3])。人参皂苷Rb1是人参根茎的重要提取物,具有抗炎,抗凋亡,能够抑制哮喘气道高反应性等作用^([4])。本研究探讨了Rb1可能通过激活AMPK/SIRT1/PGC-1α信号轴改善小鼠支气管上皮细胞在CRE诱导下发生的氧化应激线粒体动力学障碍,最终有效缓解哮喘气道炎症的发生和发展。

关键词：人参皂苷Rb1 AMPK SIRT1 PGC-1α 线粒体哮喘

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舒芬太尼对非停跳冠状动脉搭桥术大鼠模型的保护作用

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中国临床药理学杂志 2024年第4期40卷 579-583页

作者：路艳朴宗方李玲王立维苏锐承德医学院附属医院本部麻醉科河北承德067000 承德医学院附属医院检验科河北承德067000 承德医学院附属医院南区麻醉科河北承德067000 承德医学院附属医院胃肠外科河北承德067000

目的探究舒芬太尼对非停跳冠状动脉搭桥术大鼠模型中心肌细胞及肾功能的影响。方法 SD大鼠随机分为假手术组(只完成开胸穿线,不结扎冠状动脉)、模型组(构建缺血灌注模型)、低剂量实验组(构建缺血灌注模型+0.5μg·kg^(-1)舒芬太尼)、... 详细信息

目的探究舒芬太尼对非停跳冠状动脉搭桥术大鼠模型中心肌细胞及肾功能的影响。方法 SD大鼠随机分为假手术组(只完成开胸穿线,不结扎冠状动脉)、模型组(构建缺血灌注模型)、低剂量实验组(构建缺血灌注模型+0.5μg·kg^(-1)舒芬太尼)、高剂量实验组(构建缺血灌注模型+1μg·kg^(-1)舒芬太尼)。术后6 h处死小鼠,用酶联免疫吸附试验(ELISA)检测心肌细胞和肾细胞肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)的表达,用蛋白质印迹法检测心肌细胞和肾细胞凋亡相关蛋白的表达,用血清肾功能试剂盒检测血清肾功能指标。结果假手术组、模型组、低剂量实验组、高剂量实验组的心肌细胞的TNF-α表达水平分别为(50.41±6.03)、(136.61±21.73)、(102.36±12.84)和(74.21±16.32)pg·mg^(-1),心肌细胞半胱氨酸蛋白酶3(Caspase-3)表达水平分别为0.31±0.02、1.26±0.18、0.83±0.12和0.67±0.08,尿素氮(BUN)水平分别为(5.94±1.12)、(20.04±5.36)、(16.84±4.19)和(10.96±3.64)μmol·L^(-1),肾细胞TNF-α表达水平分别为(146.49±16.13)、(1 023.82±34.69)、(841.65±49.66)和(324.84±48.93)pg·mg^(-1),肾细胞Caspase-3表达水平分别为0.48±0.06、1.15±0.17、0.96±0.06和0.74±0.07。高剂量实验组与假手术组、模型组、低剂量实验组比较,在统计学上差异均有统计学意义(均P<0.05)。结论舒芬太尼减轻大鼠心肌细胞的炎症因子的生成及凋亡蛋白的表达,改善大鼠的肾功能,减轻大鼠肾细胞的炎症因子的生成及凋亡蛋白的表达。

关键词：舒芬太尼缺血再灌注心肌细胞肾功能凋亡

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TPI缺乏症斑马鱼模型的构建及分析

遗传 2024年第3期46卷 232-241页

作者：孙飘李颖刘帆王璐中国医学科学院血液病医院(中国医学科学院血液学研究所) 实验血液学国家重点实验室国家血液系统疾病临床医学研究中心细胞生态海河实验室天津300020 天津医学健康研究院天津301600

磷酸丙糖异构酶缺乏症(triosephosphate isomerase deficiency,TPI DF)是一种严重的多系统退行性疾病,通常表现为溶血性贫血、神经肌肉功能障碍和易感染,患者多于起病5年内死亡。目前尚不清楚TPI DF的具体发病机制,缺乏有效的临床治疗... 详细信息

磷酸丙糖异构酶缺乏症(triosephosphate isomerase deficiency,TPI DF)是一种严重的多系统退行性疾病,通常表现为溶血性贫血、神经肌肉功能障碍和易感染,患者多于起病5年内死亡。目前尚不清楚TPI DF的具体发病机制,缺乏有效的临床治疗方法。本研究选取TPI DF患者中最常见的突变位点TPI1^(E105D),构建了表达人源性TPI1^(E105D)(hTPI1^(E105D))的转基因斑马鱼(Danio rerio)模型[Tg(Ubi:TPI1^(E105D)-eGFP)]。功能分析表明,过表达TPI1^(E105D)影响红系及髓系细胞发育、导致神经以及肌肉发育异常。综上所述,本研究构建了磷酸丙糖异构酶缺乏症的斑马鱼疾病模型,并能够复现TPI DF患者的大部分临床表型,该模型为后续研究TPI DF的发病机制及药物筛选提供了新的实验动物模型。

关键词：磷酸丙糖异构酶缺乏症 TPI1^(E105D) 斑马鱼疾病模型

The evolving roles of Wnt signaling in stem cell proliferation and differentiation, the development of human diseases, and therapeutic opportunities

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Genes & Diseases 2024年第3期11卷 155-187页

作者： Michael Yu Kevin Qin Jiaming Fan Guozhi Zhao piao Zhao Wei Zeng Connie Chen Annie Wang Yonghui Wang jiamin Zhong Yi Zhu William Wagstaff Rex C.Haydon Hue H.Luu Sherwin Ho Michael J.Lee Jason Strelzow Russell R.Reid Tong-Chuan He School of Medicine Rosalind Franklin University of Medicine and ScienceNorth ChicagoIL 60064USA Molecular Oncology Laboratory Department of Orthopedic Surgery and Rehabilitation MedicineThe University of Chicago Medical CenterChicagoIL 60637USA Ministry of Education Key Laboratory of Diagnostic Medicine and Department of Clinical BiochemistryThe School of Laboratory MedicineChongqing Medical UniversityChongqing 400016China Department of Orthopedic Surgery The First Affliated Hospital of Chongqing Medical UniversityChongqing 400016China Department of Urology The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China Department of Clinical Laboratory Medicine Shanghai Jiaotong University School of MedicineShanghai 200000China Department of Orthopaedic Surgery Chinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100730China Laboratory of Craniofacial Suture Biology and Development Department of Surgery Section of Plastic SurgeryThe University of Chicago Medical CenterChicagoIL 60637USA Department of Neurology The First Dongguan Affiliated HospitalGuangdong Medical UniversityDongguanGuangdong 523475China

The evolutionarily conserved Wnt signaling pathway plays a central role in develop-ment and adult tissue homeostasis across *** proteins are secreted,lipid-modified signaling molecules that activate the canonical(β-catenin dependent)and non-canonical(β-catenin independent)Wnt signaling *** behaviors such as proliferation,differ-entiation,maturation,and proper body-axis specification are carried out by the canonical pathway,which is the best characterized of the known Wnt signaling *** signaling has emerged as an important factor in stem cell biology and is known to affect the self-renewal of stem cells in various *** includes but is not limited to embryonic,hematopoietic,mesenchymal,gut,neural,and epidermal stem *** signaling has also been implicated in tumor cells that exhibit stem cell-like *** signaling is crucial for bone formation and presents a potential target for the development of therapeutics for bone *** surprisingly,aberrant Wnt signaling is also associated with a wide variety of diseases,including *** of Wnt pathway members in cancer can lead to unchecked cell proliferation,epithelial-mesenchymal transition,and ***,advances in the understand-ing of dysregulated Wnt signaling in disease have paved the way for the development of novel therapeutics that target components of the Wnt *** with a brief overview of the mechanisms of canonical and non-canonical Wnt,this review aims to summarize the cur-rent knowledge of Wnt signaling in stem cells,aberrations to the Wnt pathway associated with diseases,and novel therapeutics targeting the Wnt pathway in preclinical and clinical studies.

关键词： β-Catenin Cancer Canonical Wnt Disease Non-canonical Wnt Stem cells Targeted therapy Wnt signaling

The partitioning patterns of nutrients between pods and seeds of Zanthoxylum fruits impacted by environmental factors

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Acta Geochimica 2018年第5期37卷 676-683页

作者： Hechun piao Siliang Li Zhifeng yan State Key Laboratory of Environmental Geochemistry Institute of Geochemistry Chinese Academy of Sciences Institute of Surface-Earth System Science Tianjin University

The nutritive quality in plant organs is related to the different partitioning patterns of nutrient resources among the organs under various environmental *** study examined the relationship between the nutritive quality of pods and seeds in Zanthoxylum and environmental factors, such as temperature and preciptation by using numerous samples collected from Southwest China to the East China of Shandong peninsula. The increasing accumulations of N, P and C in seeds implied that the nutritive quality in seeds was higher at the regions with relative higher mean annual temperature(MAT) and mean annual precipitation(MAP), while that in pods was on the contrary. By contrast, pod nutritive content was relatively high, but seed nutritive content was relatively low at the regions with relative high MAT and MAP. In addition, C:N ratio in pods was significantly and negatively correlated with MAT and MAP, while that in seed was significantly and positively correlated with MAT and MAP. The partitioning patterns of N-compounds between pods and seeds reflected different nitrogen translocations in the plant organs under various climate condition. The N:P ratios were negatively correlated with MAP, implying a higher proportional allocation of P to seeds than that of N in the areas with a relative high MAP. Therefore, the strategies to assess pod nutritional quality should be taken into accountfor nutritive translocation under various environmental conditions.

关键词： Partitioning pattern Nutritional quality C:Nratio Zanthoxylum fruits Mean annual temperature Mean annual precipitation

METTL14 downregulation drives S100A4^(+)monocyte-derived macrophages via MyD88/NF-κB pathway to promote MAFLD progression

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Signal Transduction and Targeted Therapy 2024年第5期9卷 2176-2192页

作者： Yue-fan Wang Wen-li Zhang Zhi-xuan Li Yue Liu Jian Tan Hao-zan Yin Zhi-chao Zhang Xian-jie piao Min-hao Ruan Zhi-hui Dai Si-jie Wang Chen-yang Mu Ji-hang Yuan Shu-han Sun Hui Liu Fu yang The Third Department of Hepatic Surgery Eastern Hepatobiliary Surgery Hospital Affiliated to Naval Medical University200438 ShanghaiChina The Department of Medical Genetics Naval Medical University200433 ShanghaiChina Translational Medicine Research Center Medical Innovation Research Division and Fourth Medical Center of the Chinese PLA General Hospital100048 BeijingChina The Department of Pharmaceutical Analysis School of PharmacyNaval Medical University200433 ShanghaiChina Key Laboratory of Biosafety Defense Ministry of Education200433 ShanghaiChina Shanghai Key Laboratory of Medical Biodefense 200433 ShanghaiChina

Without intervention,a considerable proportion of patients with metabolism‐associated fatty liver disease(MAFLD)will progress from simple steatosis to metabolism‐associated steatohepatitis(MASH),liver fibrosis,and even hepatocellular ***,the molecular mechanisms that control progressive MAFLD have yet to be fully ***,we unraveled that the expression of the N6-methyladenosine(m6A)methyltransferase METTL14 is remarkably downregulated in the livers of both patients and several murine models of MAFLD,whereas hepatocyte-specific depletion of this methyltransferase aggravated lipid accumulation,liver injury,and ***,hepatic Mettl14 overexpression alleviated the above pathophysiological changes in mice fed on a high-fat diet(HFD).Notably,in vivo and in vitro mechanistic studies indicated that METTL14 downregulation decreased the level of GLS2 by affecting the translation efficiency mediated by YTHDF1 in an m6A-depedent manner,which might help to form an oxidative stress microenvironment and accordingly recruit Cx3cr1^(+)Ccr2^(+)monocyte-derived macrophages(Mo-macs).In detail,Cx3cr1^(+)Ccr2^(+)Mo-macs can be categorized into M1-like macrophages and S100A4-positive macrophages and then further activate hepatic stellate cells(HSCs)to promote liver *** experiments revealed that CX3CR1 can activate the transcription of S100A4 via CX3CR1/MyD88/NF-κB signaling pathway in Cx3cr1^(+)Ccr2^(+)*** of METTL14 or GLS2,or interfering with this signal transduction pathway such as inhibiting MyD88 could ameliorate liver injuries and *** together,these findings indicate potential therapies for the treatment of MAFLD progression.

关键词： S100A4 MyD88 metabolism

Dichloroacetic acid and rapamycin synergistically inhibit tumor progression

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2023年第5期24卷 397-405页

作者： Huan CHEN Kunming LIANG Cong HOU hai-long piao CAS Key Laboratory of Separation Science for Analytical Chemistry Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalian 116023China Department of Biochemistry&Molecular Biology School of Life SciencesChina Medical UniversityShenyang 110122China University of Chinese Academy of Sciences Beijing 100049China

Mammalian target of rapamycin(mTOR)controls cellular anabolism,and mTOR signaling is hyperactive in most cancer *** a result,inhibition of mTOR signaling benefits cancer *** is a US Food and Drug Administration(FDA)-approved drug,a specific mTOR complex 1(mTORC1)inhibitor,for the treatment of several different types of ***,rapamycin is reported to inhibit cancer growth rather than induce *** dehydrogenase complex(PDHc)is the gatekeeper for mitochondrial pyruvate *** inactivation has been observed in a number of cancer cells,and this alteration protects cancer cells from senescence and nicotinamide adenine dinucleotide(NAD^(+))*** this paper,we describe our finding that rapamycin treatment promotes pyruvate dehydrogenase E1 subunit alpha 1(PDHA1)phosphorylation and leads to PDHc inactivation dependent on mTOR signaling inhibition in *** inactivation reduces the sensitivity of cancer cells'response to *** a result,rebooting PDHc activity with dichloroacetic acid(DCA),a pyruvate dehydrogenase kinase(PDK)inhibitor,promotes cancer cells'susceptibility to rapamycin treatment in vitro and in vivo.

关键词： Dichloroacetic acid(DCA) Rapamycin Pyruvate dehydrogenase E1 subunit alpha 1(PDHA1) Mammalian target of rapamycin(mTOR)

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离子色谱法测定地下水中碘离子的方法研究

福建分析测试 2024年第2期33卷 28-30,34页

作者：黄漂漂福建省漳州环境监测中心站福建漳州363000

采用离子色谱法测定地下水中碘离子。选择AS19分离柱、电导检测器、阴离子电解再生抑制器电流为99 mA、淋洗液为40 mmol/L KOH溶液、流速为1.00 mL/min、检测器温度为30℃、进样定量环为250μL。碘离子浓度在0.010 mg/L~1.00 mg/L的范... 详细信息

采用离子色谱法测定地下水中碘离子。选择AS19分离柱、电导检测器、阴离子电解再生抑制器电流为99 mA、淋洗液为40 mmol/L KOH溶液、流速为1.00 mL/min、检测器温度为30℃、进样定量环为250μL。碘离子浓度在0.010 mg/L~1.00 mg/L的范围内与色谱峰面积具有良好的线性关系,其相关系数可以达到0.9998,方法检出限为0.001 mg/L,相对标准偏差为0.4%~8.3%,加标回收率为99.0%~115.0%,有证标准物质分析结果优秀,相对偏差为2.5%。将其应用于地下水中碘离子的测定,结果满意。

关键词：离子色谱法碘离子地下水测定