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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2011年第3期12卷 193-200页

作者： Shu-fang WANG hai-yan fang hai-bin QU Pharmaceutical Informatics Institute Zhejiang University Hangzhou 310058 China

Central composite design(CCD),together with multiple linear regression,was successfully used to optimize the electrophoretic buffer system of micellar electrokinetic capillary chromatography(MEKC) for the determination of albiflorin,paeoniflorin,liquiritin,and glycyrrhizic acid in the traditional Chinese medicine(TCM) prescription,yangwei *** of sodium deoxycholate(SDC) and borate,and proportions of ammonia,acetonitrile,and methanol were *** total resolutions of peaks between the analytes and their adjacent peaks in real samples were integrated into the evaluation index of separation *** optimum electrophoretic buffer contained 80 mmol/L SDC,20 mmol/L borate,5%(v/v) methanol,0.5%(v/v) ammonia,and 5%(v/v) *** correlation coefficients(R 2 ) between the peak areas and the corresponding concentrations of analytes were greater than *** limits of detection(LODs) (S/N=3) of the analytes were 0.97-4.00μg/*** results indicate the superiority of CCD in optimizing the separation conditions of complex samples such as TCM prescriptions.

关键词： Micellar electrokinetic capillary chromatography Traditional Chinese medicine Central composite design yangwei granule

A novel period estimation method for X-ray pulsars based on frequency subdivision

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Frontiers of Information Technology & Electronic Engineering 2015年第10期16卷 858-870页

作者： Li-rong SHEN Xiao-ping LI hai-feng SUN hai-yan fang Meng-fan XUE School of Aerospace Science and Technology Xidian University

Period estimation of X-ray pulsars plays an important role in X-ray pulsar based navigation （XPNAV）. The fast Lomb periodogram is suitable for period estimation of X-ray pulsars, but its performance in terms of frequency resolution is limited by data length and observation time. Longer observation time or oversampling can be employed to improve frequency analysis results, but with greatly increased computational complexity and large amounts of sampling data. This greatly restricts real-time autonomous navigation based on X-ray pulsars. To resolve this issue, a new method based on frequency subdivision and the continuous Lomb periodogram （CLP） is proposed to improve precision of period estimation using short-time observation data. In the proposed method, an initial frequency is first calculated using fast Lomb periodogram. Then frequency subdivision is per- formed near the initial frequency to obtain frequencies with higher precision. Finally, a refined period is achieved by calculating the CLP in the obtained frequencies. Real data experiments show that when observation time is shorter than 135 s, the proposed method improves period estimation precision by 1-3 orders of magnitude compared with the fast Lomb periodogram and fast Fourier transform （FFT） methods, with only a slight increase in computational complexity. Furthermore, the proposed method performs better than efsearch （a period estimation method of HEAsoft） with lower computational complexity. The proposed method is suitable for estimating periods of X-ray pulsars and obtaining the rotation period of variable stars and other celestial bodies.

关键词： Pulsar navigation Period estimation Frequency subdivision Continuous Lomb periodogram

Effects of lower fluence pulsed dye laser irradiation on production of collagen and the mRNA expression of collagen relative gene in cultured fibroblasts in vitro

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Chinese Medical Journal 2006年第18期119卷 1543-1547页

作者： YU hai-yan CHEN Da-fang WANG Qi CHENG Hao Department of Dermatology Sir Run Run Shaw Hospital of Zhejiang University Hangzhou 310016 China

Background Lower fluence of 585-nm flashlamp-pumped pulsed dye laser has been successfully used as a nonablative technique in the treatment of wrinkles. The objective of this study was to evaluate the effect of the pulsed dye laser （585 nm） on the production of collagen and the mRNA expression of collagen related gene in fibroblasts in vitro. Methods Cultured fibroblasts were treated with a 585-nm flashlamp-pumped pulsed dye laser （ fluence 3 J/cm^2, 4 J/cm^2, spot size 7 mm, pulse duration 450 12s）. The production of collagen and the mRNA expression of transforming growth factor （TGF）-β1, SMAD2, SMAD3, SMAD4, SMAD7 and type Ⅰ procollagen α1, α2 in fibroblasts were investigated by colorimetry or real time polymerase chain reaction. Results The production of collagen was significantly up-regulated after treatment with a 585-nm flashlamp-pumped pulsed dye laser with a fluence of 3 J/cm^2 （P 〈0.001）. The mRNA expression of TGF- β1, SMAD2, SMAD3, SMAD4, SMAD7 and procollagen I was significantly up-regulated after treatment with a 585-nm flashlamp-pumped pulsed dye laser with a fluence of 3 J/cm^2 （P 〈0.001）. No significant difference of mRNA expression of SMAD2, SMAD3, SMAD4, SMAD7 and type Ⅰ procollagen was found between controls and fibroblasts treated with pulsed dye laser with a fluence of 4 J/cm^2 （P 〉0.05）. Conclusions Lower fluence （3 J/cm^2） pulsed dye laser increased the collagen production in fibroblasts by up-regulating TGF-β1, SMAD2, SMAD3, SMAD4, SMAD7 and type Ⅰ procollagen mRNA expression. These may be the reason it can be effectively used in the treatment of wrinkles.

关键词： pulsed dye laser fibroblasts procollagen SMADs

Building the Crab pulsar timing model with XPNAV-1 observations

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Building the Crab pulsar timing model with XPNAV-1 observati...

定数截尾混合指数分布在恒加应力下的MCEM加速算法

第九届中国卫星导航学术年会

作者： hai-feng Sun Jian-yu Su Liang Zhao Zhi-wei Liu hai-yan fang School of Aerospace Science and Technology Xidian University Science and Technology on Space Physics Laboratory Beijing Institute of Astronautical Systems Engineering

This paper addresses the problem of building the X-ray pulsar timing model that is an essential work for X-ray pulsar navigation(XNAV).We propose a theoretical discussion of the differences between the X-ray and radio pulsar timing and develop a complete processing *** this framework,the 2 mZ-test method is deduced by the theory of the Fourier Series Estimator(FSE) in the complex form.A fast maximum likelihood(FAML) estimator is applied for the estimation of pulse time of arrival(TOA).Then the model parameters are deduced based on a least-squares *** sustain this framework,about 173 ks observations with X-ray pulsar navigation-I(XPNAV-1) satellite over 30 days are utilized for experimental *** results show that the proposed framework is valid,and the phase residuals with the X-ray model parameters is 2.57 milliperiods.

关键词： X-ray pulsar navigation X-ray timing model Crab pulsar XPNAV-1 satellite

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数学的实践与认识 2012年第1期24卷 127-134页

作者：严海芳蒋卉青海大学基础部青海西宁810016 青海师范大学数学与信息科学系系青海西宁810008

应用Monte Carlo EM加速算法给出了混合指数分布在恒加应力水平下,在定数截尾场合的参数估计问题,并通过模拟试验说明利用Monte Carlo EM加速算法来估计混合指数分布比EM算法更有效,收敛速度更快.

关键词：混合指数分布 Monte Carlo EM加速算法恒加应力加速寿命试验截尾数据

红穗和白穗醋栗F3H的克隆及其在果实成熟过程中的表达分析

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园艺学报 2016年第10期43卷 2003-2011页

作者：王海竹闫海芳徐启江东北林业大学林木遗传育种国家重点实验室哈尔滨150040 东北林业大学生命科学学院哈尔滨150040

以红穗醋栗(Ribes rubrum L.)和白穗醋栗(*** L.)果实为试材,采用RACE方法克隆黄烷酮3–羟化酶(F3H)基因c DNA全长序列,分别命名为Rr F3H和Ra F3H(KU984435和KU984436)。Rr F3H全长1 351 bp,开放阅读框长1 098 bp,编码365个氨基酸;Ra F3... 详细信息

以红穗醋栗(Ribes rubrum L.)和白穗醋栗(*** L.)果实为试材,采用RACE方法克隆黄烷酮3–羟化酶(F3H)基因c DNA全长序列,分别命名为Rr F3H和Ra F3H(KU984435和KU984436)。Rr F3H全长1 351 bp,开放阅读框长1 098 bp,编码365个氨基酸;Ra F3H全长1 291 bp,开放阅读框长1 098 bp,编码365个氨基酸。氨基酸多序列比对表明该基因编码的蛋白具有非血红素双加氧酶结构域(DIOX-N superfamily)和典型的F3H蛋白功能结构域(2OG-FeⅡ_Oxy加氧酶结构域),属于双加氧酶超家族。系统发育分析表明,Rr F3H和Ra F3H在进化上具有明显种属特性,属于相对独立的进化分支。定量PCR分析表明,F3H在红穗醋栗中表达量远远高于白穗醋栗,在红穗醋栗中随着果实着色加深表达量逐渐上升,果实着色约75%时表达量最高,之后下降;白穗醋栗中随着果实生长,该基因的表达下降,花色苷含量也呈下降趋势,说明F3H基因在醋栗果实着色过程中发挥作用。

关键词：红穗醋栗白穗醋栗花色苷 F3H 基因克隆

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混合指数分布恒加应力下的MCEM加速算法

湘潭大学自然科学学报 2011年第3期33卷 35-37,60页

作者：严海芳蒋卉青海大学青海西宁810016 青海师范大学青海西宁810008

应用Monte Carlo EM(简称MCEM)加速算法改进了混合指数分布在恒加应力水平下的参数估计,并通过模拟试验说明利用Monte Carlo EM加速算法来估计混合指数分布比EM算法更有效,收敛速度更快.

关键词：混合指数分布 MonteCarloEM加速算法恒加应力加速寿命试验完全数据

Engineered Cancer Nanovaccines:A New Frontier in Cancer Therapy

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Nano-Micro Letters 2025年第2期17卷 134-168页

作者： Yijie Wang Congrui Liu Chao fang Qiuxia Peng Wen Qin Xuebing yan Kun Zhang Central Laboratory and Department of Medical Ultrasound Sichuan Academy of Medical SciencesSichuan Provincial People’s HospitalSchool of MedicineUniversity of Electronic Science and Technology of ChinaNo.32West Second SectionFirst Ring RoadChengdu 610072People’s Republic of China Department of Stomatology and Central Laboratory School of MedicineShanghai Tenth People’s HospitalTongji UniversityNO.301 Yan‑Chang‑Zhong RoadShanghai 200072People’s Republic of China Jiangsu Provincial Innovation and Practice Base for Postdoctors Suining People’s HospitalAffiliated Hospital of Xuzhou Medical UniversityNo.2Bayi West RoadSuiningXu Zhou 221000Jiangsu ProvincePeople’s Republic of China

Vaccinations are essential for preventing and treating disease,especially cancer nanovaccines,which have gained considerable interest recently for their strong anti-tumor immune *** can prompt the immune system to generate antibodies and activate various immune cells,leading to a response against tumor tissues and reducing the negative effects and recurrence risks of traditional chemotherapy and *** enhance the flexibility and targeting of vaccines,nanovaccines utilize nanotechnology to encapsulate or carry antigens at the nanoscale level,enabling more controlled and precise drug delivery to enhance immune *** nanovaccines function by encapsulating tumor-specific antigens or tumor-associated antigens within *** small size of these nanomaterials allows for precise targeting of T cells,dendritic cells,or cancer cells,thereby eliciting a more potent anti-tumor *** this paper,we focus on the classification of carriers for cancer nanovaccines,the roles of different target cells,and clinically tested cancer nanovaccines,discussing strategies for effectively inducing cytotoxic T lymphocytes responses and optimizing antigen presentation,while also looking ahead to the translational challenges of moving from animal experiments to clinical trials.

关键词： Cancer nanovaccines Immunotherapy Nanobiotechnology Immune targets Signaling pathway

Double [5]carbohelicene: facile synthesis, chiroptical properties,isomerization study, and lasing application

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Science China Chemistry 2025年第1期68卷 233-240页

作者： Peipei Liu yantong Li Meng-Xiang Wu Hao Kang Xiao-Li Zhao Lin Xu Linlin Liu Xiaodong Li Junfeng fang Zhiwei fang Ya Cheng hai-Bo yang Huakang Yu Xueliang Shi State Key Laboratory of Petroleum Molecular & Process Engineering Shanghai Key Laboratory of Green Chemistry and Chemical ProcessesSchool of Chemistry and Molecular Engineering East China Normal University School of Physics and Optoelectronics South China University of Technology Institute of Polymer Optoelectronic Materials & Devices State Key Laboratory of Luminescent Materials & Devices South China University of Technology School of Physics and Electronic Science Engineering Research Center of Nanophotonics & Advanced Instrument Ministry of EducationEast China Normal University State Key Laboratory of Precision Spectroscopy School of Physics and Electronic Science East China Normal University

Double carbohelicenes have attracted considerable attention due to their aesthetic structures, distinct π-conjugation extension, inherent chirality, and intriguing optical and electronic properties. Herein, the concise de novo synthesis of a new double[5]carbohelicene 1 together with its chiroptical properties, isomerization process and lasing application is presented. 1 was synthesized by a simple protocol involving the formation of a 6,6′-bipentacene as the key intermediate. It is worth noting that1 existed as two diastereomers 1-PP/MM and 1-PM, which were successfully isolated and unambiguously confirmed by Xray crystallography. The optical resolution of the racemic 1-PP/MM was successfully achieved by chiral-phase highperformance liquid chromatography（HPLC）, and 1-PP and 1-MM were characterized by circular dichroism. Interestingly, it was found that 1-PP/MM could be completely isomerized to 1-PM upon heating, and the detailed thermodynamics and kinetics of the isomerization process were systematically investigated. 1-PM exhibited deep-red luminescence with emission maximum at 723 nm and fluorescence quantum yield as high as 32.95%. Consequently, we can showcase the lasing application of 1-PM by doping with epoxy resin to assemble a bottle microlaser spontaneously, which the lasing wavelength is 796.6 nm at a threshold of 0.8 m J cm-2. We believe that our studies, including the facile synthesis methodology, detailed isomerization studies, and lasing application, will shed some light on the design and synthesis of novel carbohelical systems with more functions.

关键词： double carbohelicene optical resolution organic laser isomerization X-ray diffraction

High Fe‑Loading Single‑Atom Catalyst Boosts ROS Production by Density Effect for Efficient Antibacterial Therapy

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Nano-Micro Letters 2025年第2期17卷 187-203页

作者： Si Chen fang Huang Lijie Mao Zhimin Zhang Han Lin Qixin yan Xiangyu Lu Jianlin Shi Department of Cardiology Shanghai Tenth People’s HospitalSchool of MedicineTongji UniversityShanghai 200092People’s Republic of China Shanghai Institute of Ceramics Chinese Academy of SciencesShanghai 200050People’s Republic of China Shanghai Frontiers Science Center of Nanocatalytic Medicine School of MedicineTongji UniversityShanghai 200092People’s Republic of China

The current single-atom catalysts(SACs)for medicine still suffer from the limited active site ***,we develop a synthetic method capable of increasing both the metal loading and mass-specific activity of SACs by exchanging zinc with *** constructed iron SACs(h^(3)-FNC)with a high metal loading of 6.27 wt%and an optimized adjacent Fe distance of~4 A exhibit excellent oxidase-like catalytic performance without significant activity decay after being stored for six months and promising antibacterial ***,a“density effect”has been found at a high-enough metal doping amount,at which individual active sites become close enough to interact with each other and alter the electronic structure,resulting in significantly boosted intrinsic activity of single-atomic iron sites in h^(3)-FNCs by 2.3 times compared to low-and medium-loading ***,the overall catalytic activity of h^(3)-FNC is highly improved,with mass activity and metal mass-specific activity that are,respectively,66 and 315 times higher than those of commercial Pt/*** addition,h^(3)-FNCs demonstrate efficiently enhanced capability in catalyzing oxygen reduction into superoxide anion(O_(2)·^(−))and glutathione(GSH)*** in vitro and in vivo assays demonstrate the superior antibacterial efficacy of h^(3)-FNCs in promoting wound *** work presents an intriguing activity-enhancement effect in catalysts and exhibits impressive therapeutic efficacy in combating bacterial infections.

关键词： Nanocatalytic medicine Single-atom catalysts Reactive oxygen species(ROS) High metal loading Oxidase catalysis