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Association between complement factor I gene polymorphisms and the risk of age-related macular degeneration:a Meta-analysis of literature

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International Journal of Ophthalmology(English edition) 2016年第2期9卷 298-305页

作者： Qin Wang hai-sheng zhao Li Li Department of Ophthalmology the Second Affiliated Hospital of Chongqing Medical University Center of Ophthalmology the Second Affiliated Hospital of Chongqing Medical University

AIM： To systematically review the association between complement factors I （CFI） polymorphisms and age- related macular degeneration （AMD） and to explore whether CFI polymorphisms are associated with AMD, METHODS： Meta-analysis of articles published from 1995 to January 2015 of articles involved with AMD and polymorphisms of the CFI gene. Eligible data were pooled in a Meta-analysis, analyzing using STATA software （version 12.0）, Review Manager （version 5.2） and different models based on the heterogeneity of effect sizes. Egger＇s test, Begg＇s rank correlation methods were used to evaluate for publication bias.~ RESULTS： Thirteen articles were eligible, describing two loci polymorphisms of the CFI gene （of which 12 articles focus on rs10033900T〉C and 3 articles focus on rs2285714C〉T）. For rs10033900T〉C, the results of our study revealed that having a mutant allele C, TC, CC and TC＋CC was associated with a decreased risk of AMD in all population groups studied （C versus T models, OR=0.84, 95%Ch 0.72-0.99, P=0.04; TC versus TT models OR= 0.89, 95%Ch 0.88-0.99, P=0.04;CC versus ＂1-1＂ models, OR=0.76, 95%Ch 0.60-0.98, P=-0.03; TC＋CC versus TT models, OR=0.81, 95%Ch0.65-0.99, P=0.04）. We found that C allele were related to lower AMD risk in the Caucasian population by subgroup analysis, but there was no association with AMD under the allele and genotypes comparison in Asian studies. For rs2285714 C〉T, the TC, TT genotypes contributed to a higher risk of AMD, compared with the CC carriers and CT＋CC （OR=1.34, 95%Ch 1.09-1.63, P=0.004; OR=1.50, 95%Ch 1.25-1.80, P〈0.0001）. CONCLUSION： This Meta-analysis suggests that CFI rs10033900T〉C and rs2285714C〉T polymorphisms may contribute to AMD.

关键词： complement factors I age-related maculardegeneration age-related maculopathy single-nucleotidepolymorphisms Meta-analysis

The performance of out-of-time events of the follow-up X-ray telescope onboard Einstein probe

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Radiation Detection Technology and Methods 2024年第2期8卷 1148-1154页

作者： hai-sheng zhao Juan Zhang Ju Guan Cheng-Kui Li Shu-Mei Jia Li-Ming Song Key Laboratory of Particle Astrophysics Institute of High Energy PhysicsChinese Academy of Sciences19B Yuquan RoadBeijing100049People’s Republic of China

Purpose:The purpose of this study is to investigate the performance of out-of-time events(OoT events)in the Follow-up X-ray Telescope(FXT)onboard the Einstein *** OoT events,registered on the PN-CCD during its readout time,can provide information on the sources,e.g.,spectral indices,and diagnose the behavior of ***:In order to analyze the characteristics of OoT events,we simulated sources,each of which had been modeled using an absorbed power law combined with Gaussians,in different FXT modes and different *** allowed us to determine the OoT spectral indices and pile-up ***:In the timing mode of FXT,the OoT events occupy a large proportion and the OoT correction must be done with the assumption that all photons originate from the source position on the *** for some bright sources,the OoT events may be sufficient to derivate the source spectra,especially when pile-up occurs,particularly in the full frame mode and the partial window mode of ***:We conclude that the OoT events can provide additional information about the celestial sources and can be used to estimate whether the pile-up occurs or not and the scope of ***,they can be also used to determine the new sources and improve the detection significance of a source.

关键词： Out-of-time Event PN-CCD Pile-up

Long-term evolution of the X-ray flux of the Crab pulsar

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Radiation Detection Technology and Methods 2023年第1期7卷 48-55页

作者： hai-sheng zhao Ming-Yu Ge Xiao-Bo Li Lin-Li Yan You-Li Tuo Fang-Jun Lu Li-Ming Song Cong-Zhan Liu Shu Zhang Shuang-Nan Zhang Key Laboratory for Particle Astrophysics Institute of High Energy PhysicsChinese Academy of Sciences19B Yuquan RoadBeijing 100049China School of Mathematics and Physics Anhui JianZhu UniversityHefei 230601AnhuiChina

Purpose In order to carry out in-orbit calibration,the hard X-ray modulation telescope satellite(HXMT)has made a number of observations of Crab *** study the long-term evolution of the X-ray flux of the Crab pulsar with these observational *** We use nearly four years Crab pulsar’s data obtained by HXMT and the neutron star interior composition of explorer to test whether L_(x)∝L_(sd)^(α) holds for an individual pulsar,where L_(x) is the pulsed X-ray luminosity and Lsd is its spin-down *** also combine the earlier results obtained with PCA and HEXTE data(Yan et *** ApJ 865:21,2018)to get the long-term evolution characteristics of the Crab *** We find the X-ray flux evolution can be fitted by a linear correlation:L_(x)∝L_(sd)^(1.42±0.18).In soft X-ray energy band(1–10 keV)and hard X-ray energy band(27–250 keV),αdoes not change significantly with either energy or ***,this will need to be confirmed in *** example,the interpulse component of hard X-ray may follow L_(x)∝L_(sd)^(0.96±0.34),which may be different from the correlation of soft X-rays and main pulse *** the other hand,the hard X-ray flux evolution shows that the performance of high energy X-ray telescope(HE)onboard HXMT,such as effective area,is *** We conclude that the X-ray luminosity of the Crab pulsar closely correlates with its spin-down ***,the emission mechanism responsible for these phenomena remains not fully understood.

关键词： X-rays Crab pulsar Flux evolution Spin-down luminosity

Subretinal fibrosis secondary to neovascular age-related macular degeneration:mechanisms and potential therapeutic targets

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Neural Regeneration Research 2025年第2期20卷 378-393页

作者： Jingxiang Zhang Xia sheng Quanju Ding Yujun Wang Jiwei zhao Jingfa Zhang Department of Ophthalmology People’s Hospital of Huangdao DistrictQingdaoShandong ProvinceChina Department of Urology People’s Hospital of Huangdao DistrictQingdaoShandong ProvinceChina Department of Ophthalmology Shanghai General Hospital(Shanghai First People’s Hospital)Shanghai Jiao Tong University School of MedicineShanghaiChina National Clinical Research Center for Eye Diseases Shanghai Key Laboratory of Ocular Fundus DiseasesShanghai Engineering Center for Visual Science and PhotomedicineShanghai Engineering Center for Precise Diagnosis and Treatment of Eye DiseasesShanghaiChina

Subretinal fibrosis is the end-stage sequelae of neovascular age-related macular *** causes local damage to photoreceptors,retinal pigment epithelium,and choroidal vessels,which leads to permanent central vision loss of patients with neovascular age-related macular *** pathogenesis of subretinal fibrosis is complex,and the underlying mechanisms are largely ***,there are no effective treatment options.A thorough understanding of the pathogenesis of subretinal fibrosis and its related mechanisms is important to elucidate its complications and explore potential *** current article reviews several aspects of subretinal fibrosis,including the current understanding on the relationship between neovascular age-related macular degeneration and subretinal fibrosis;multimodal imaging techniques for subretinal fibrosis;animal models for studying subretinal fibrosis;cellular and non-cellular constituents of subretinal fibrosis;pathophysiological mechanisms involved in subretinal fibrosis,such as aging,infiltration of macrophages,different sources of mesenchymal transition to myofibroblast,and activation of complement system and immune cells;and several key molecules and signaling pathways participating in the pathogenesis of subretinal fibrosis,such as vascular endothelial growth factor,connective tissue growth factor,fibroblast growth factor 2,platelet-derived growth factor and platelet-derived growth factor receptor-β,transforming growth factor-βsignaling pathway,Wnt signaling pathway,and the axis of heat shock protein 70-Toll-like receptors 2/*** review will improve the understanding of the pathogenesis of subretinal fibrosis,allow the discovery of molecular targets,and explore potential treatments for the management of subretinal fibrosis.

关键词： choroidal neovascularization epithelial-mesenchymal transition mesenchymal transition myofibroblast neovascular age-related macular degeneration submacular fibrosis subretinal fibrosis therapeutic targets transforming growth factor-β vascular endothelial growth factor

Paired quasi-periodic pulsations of hard X-ray emission in a solar flare

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Science China(Physics,Mechanics & Astronomy) 2023年第5期66卷 195-206页

作者： hai-sheng zhao Dong Li Shao-Lin Xiong Yan-Qiu Zhang Yang Su Wei Chen Yi zhao Xiao-Bo Li Jia-Cong Liu Wen-Xi Peng Rui Qiao Xin-Qiao Li Xiang-Yang Wen Li-Ming Song Shi-Jie Zheng Xin-Ying Song Xiao-Yun zhao Yue Huang Shuang-Nan Zhang Shuo Xiao Ce Cai Zheng-Hua An Can Chen Gang Chen Wei Chen Yan-Qi Du Min Gao Ke Gong Dong-Ya Guo Zhi-Wei Guo Jian-Jian He Bin Li Chao Li Chao-Yang Li Gang Li Jian-Hui Li Lu Li Qing-Xin Li Yan-Guo Li Jing Liang Xiao-Hua Liang Jin-Yuan Liao Xiao-Jing Liu Ya-Qing Liu Qi Luo Xiang Ma Bin Meng Ge Ou Dong-Li Shi Jing-Yan Shi Gong-Xing Sun Xi-Lei Sun You-Li Tuo Chen-Wei Wang Hui Wang Jin Wang Jin-Zhou Wang Ping Wang Wen-Shuai Wang Hong Wu sheng-Lun Xie Yan-Bing Xu Yu-Peng Xu Wang-Chen Xue sheng Yang Min Yao Jian-Ying Ye Qi-Bin Yi Chao-Yue Zhang Da-Li Zhang Fan Zhang Fei Zhang Hong-Mei Zhang Kai Zhang Peng Zhang Xiao-Lu Zhang Zhen Zhang Guo-Ying zhao Shi-Yi zhao Chao Zheng Xing Zhou Yue Zhu Key Laboratory for Particle Astrophysics Institute of High Energy PhysicsChinese Academy of SciencesBeijing 100049China Purple Mountain Observatory Chinese Academy of SciencesNanjing 210023China University of Chinese Academy of Sciences Beijing 100049China Department of Astronomy Beijing Normal UniversityBeijing 100875China School of Computing and Artificial Intelligence Southwest Jiaotong UniversityChengdu 611756China Physics and Space Science College China West Normal UniversityNanchong 637002China Key Laboratory of Stellar and Interstellar Physics and Department of Physics Xiangtan UniversityXiangtan 411105China Institute of Astrophysics Central China Normal UniversityWuhan 430079China School of Physics Changchun University of Science and TechnologyChangchun 130022China College of Physics and Engineering Qufu Normal UniversityQufu 273165China College of Physics Sciences&Technology Hebei UniversityBaoding 071002China

We investigate high time resolution data obtained by the Gravitational wave high-energy Electromagnetic Counterpart All-sky Monitor(GECAM)during the flare event on 2022 April 21 at 01:52 *** subpeaks with durations of 4-6 s have been detected in the hard X-ray precursor phase,and the key feature is that they appear in pairs and seem like double-peak *** subpeaks are rarely observed in hard X-ray band and confirmed by the microwave obtained by Nobeyama Radio Polarimeters(NoRP)and Radio Solar Telescope Network(RSTN).While an exponential function can describe the continuum component of the time profile from the precursor to part of the impulsive *** periods of quasi-periodic pulsations(QPPs)are detected to be about 7.3 and 12.8 s for the precursor and impulsive phase,respectively,with at least 95%confidence *** paired QPPs are assumed to be double-peak QPPs and then the scenario of current loop coalescence model is found to be in good agreement with our *** precursor phase can be interpreted as the oscillating coalescence of two islands,while the impulsive phase can be interpreted as more islands to coalesce one by one to form larger islands.

关键词： solar flare QPPs loop coalescence circular-ribbon flare

浅谈临床寰枢关节旋转式半脱位诊治过程中有待商榷的几个部分

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医学争鸣 2016年第6期37卷 18-19,24页

作者：袁海光赵胜杰赵文娟鲁刚陕西中医药大学附属医院陕西咸阳712000

寰枢关节旋转半脱位是颈椎病中最常见的损伤,由于寰枢关节的解剖位置和功能的复杂性,相应的临床症状也相当复杂且严重。若未经及时治疗,其脱位程度常进行性加重,导致脊髓高位受压而危及生命。由于其潜在危险性大,因此关于寰枢关节的诊... 详细信息

寰枢关节旋转半脱位是颈椎病中最常见的损伤,由于寰枢关节的解剖位置和功能的复杂性,相应的临床症状也相当复杂且严重。若未经及时治疗,其脱位程度常进行性加重,导致脊髓高位受压而危及生命。由于其潜在危险性大,因此关于寰枢关节的诊断治疗存在诸多争论。本文从问诊与查体、影像学检查及治疗这三个方面阐述目前所存在的争议,力求更精确的诊断与治疗。

关键词：寰枢关节旋转半脱位颈椎病影像学检查

rhLEDGF p52基因原核表达载体的构建、诱导表达及蛋白纯化(英文)

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国际眼科杂志 2006年第4期6卷 751-754页

作者：赵海生王一第三军医大学西南医院眼科中国重庆市400038

目的 :构建 rhLEDGF p52 基因原核表达载体 , 获得rhLEDGF p52 蛋白。方法:利用基因重组技术将 rhLEDGF p52 基因构建于原核表达载体 pET30a(+)中,经酶切、测序鉴定证实构建完全正确后,通过 IPTG 诱导在大肠杆菌 BL21(DE3)中获得表达,... 详细信息

目的 :构建 rhLEDGF p52 基因原核表达载体 , 获得rhLEDGF p52 蛋白。方法:利用基因重组技术将 rhLEDGF p52 基因构建于原核表达载体 pET30a(+)中,经酶切、测序鉴定证实构建完全正确后,通过 IPTG 诱导在大肠杆菌 BL21(DE3)中获得表达,经免疫蛋白印迹试验对 rhLEDGF p52 进行鉴定并用 Ni-NTA *** 方法进行纯化。结果:成功构建 rhLEDGF p52 基因原核表达载体,在大肠杆菌 BL21(DE3)中获得可溶性形式表达,rhLEDGF p52蛋白表达量占菌体蛋白总量的 34.63%。Western Blot 结果显示 rhLEDGF2 蛋白能够特异性与 LEDGF-ab 结合。Ni-NTA *** 方法进行纯化后的 rhLEDGF p52,终浓度达 520mg/L,分析其纯度达 87.93%。结论:获得 rhLEDGF p52 蛋白,这为深入研究其生物学功能奠定了基础。

关键词：人类晶状体上皮来源的生长因子p52 基因表达大肠杆菌蛋白纯化

细菌内同源重组法构建LEDGFp52基因腺病毒载体及体外表达(英文)

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国际眼科杂志 2006年第5期6卷 979-983页

作者：赵海生王一第三军医大学西南医院眼科中国重庆市400038

目的: 构建表达人类晶状体来源的上皮生长因子 p52(LEDGFp52)重组腺病毒载体,检测 LEDGFp52 重组腺病毒能否在真核细胞中有效表达目的基因。方法: 将 LEDGFp52 基因片段亚克隆到腺病毒穿梭质粒pAdTrack- CMV上构建腺病毒穿梭载体 pAdTra... 详细信息

目的: 构建表达人类晶状体来源的上皮生长因子 p52(LEDGFp52)重组腺病毒载体,检测 LEDGFp52 重组腺病毒能否在真核细胞中有效表达目的基因。方法: 将 LEDGFp52 基因片段亚克隆到腺病毒穿梭质粒pAdTrack- CMV上构建腺病毒穿梭载体 pAdTrack- CMV-LEDGFp52,将腺病毒穿梭载体 pAdTrack- CMV- LEDGFp52 与5 型腺病毒骨架质粒 pAdeasy- 1 共转染 BJ5183 细菌, 经细菌内同源重组产生携带 LEDGFp52 基因的重组腺病毒载体 pAd- LEDGFp52, 将 pAd- LEDGFp52 经脂质体法转化293 细胞包装产生重组腺病毒 Ad- LEDGFp52, 将 Ad-LEDGFp52 体外转染 293 细胞, CPE 法及 westernblot 观察目的基因的表达。结果: 构建了表达 LEDGFp52 基因的重组腺病毒质粒, *** 内成功同源重组腺病毒, 在 293 细胞内包装产生重组腺病毒 Ad- LEDGFp52, 滴度可达 5×1012 pfu/L。在真核细胞中有效表达目的基因 LEDGFp52, 出现显著的 CPE 效应。结论: 成功构建表达 LEDGFp52 的重组腺病毒载体, 为进一步进行 LEDGF p52 基因功能的研究提供了实验基础。

关键词： LEDGFp52基因腺病毒载体体外表达

LEDGFp52-siRNA真核表达载体的构建和鉴定(英文)

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国际眼科杂志 2006年第5期6卷 975-978页

作者：赵海生王一第三军医大学西南医院眼科中国重庆市400038

目的: 构建 LEDGFp52 基因 RNA 干扰(RNAi)的真核细胞表达载体。方法: 以 LEDGFp52 为靶基因, 以 pGenSil-l 质粒为载体, 设计构建重组体, 根据 GenBank 数据库提供的LEDGFp52 基因核苷酸序列, 按照 Tuschl 设计原则, 选择设计两条带发... 详细信息

目的: 构建 LEDGFp52 基因 RNA 干扰(RNAi)的真核细胞表达载体。方法: 以 LEDGFp52 为靶基因, 以 pGenSil-l 质粒为载体, 设计构建重组体, 根据 GenBank 数据库提供的LEDGFp52 基因核苷酸序列, 按照 Tuschl 设计原则, 选择设计两条带发夹结构的核苷酸序列, 克隆到空载体pGenSil-l 中, 转化 DH5α菌株, 提取质粒, 进行限制性内切酶酶切鉴定和测序分析。结果: 经酶切鉴定筛选出的重组体测序结果与目的序列完全一致, 重组载体构建成功,重组质粒转染 HeLa 细胞48h, Western blotting 检测到 LEDGFp52 蛋白表达的改变。结论: 利用 RNAi 技术可成功构建抑制 LEDGFp52 表达的小干扰 RNA 重组体。

关键词： LEDGFp52 RNA干扰真核表达载体构建鉴定