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Cancer Communications 2019年第1期39卷 576-578页

作者： Li-Ting Liu Melvin L.K.Chua Yungan Tao Lin-Quan Tang hai-qiang mai Sun Yat-sen University Cancer Center State Key Laboratory of Oncology in South China Collaborative Innovation Center for Cancer Medicine Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and TherapyGuangzhou 510060GuangdongP.R.China Department of Nasopharyngeal Carcinoma Sun Yat-sen University Cancer Center651 Dongfeng Road EastGuangzhou 510060GuangdongP.R.China Divisions of Radiation Oncology and Medical Sciences National Cancer Centre SingaporeSingapore 169610Singapore Oncology Academic Programme Duke-National University of Singapore Medical SchoolSingapore 169857Singapore.5 Institute Gustave-Roussy94800 VillejuifFrance

main text In the past decades,there have been several studies con-cerning the efficacy of sequencing of chemotherapy on disease control and survival in locoregionally advanced(LA)nasopharyngeal carcinoma(NPC).The addition of concurrent cisplatin to radiotherapy has demonstrated survival improvements that are attributable to both dis-tant metastasis and locoregional *** to the latter,the advent of intensity-modulated radiotherapy has resulted in superior tumor control given the bet-ter dosimetry compared to conventional techniques[1].However,distant recurrence still occurs in 20-30%patients and accounts for the main cause of *** address this,several groups have explored the advantages of adding neoadjuvant chemotherapy(NACT)or adju-vant chemotherapy(ACT)to the backbone of concurrent chemoradiotherapy(CCRT).

关键词： chemotherapy nasopharyngeal metastasis

The role of capecitabine as maintenance therapy in de novo metastatic nasopharyngeal carcinoma:A propensity score matching study

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Cancer Communications 2020年第1期40卷 32-42页

作者： Xue-Song Sun Sai-Lan Liu Yu-Jing Liang Qiu-Yan Chen Xiao-Yun Li Lin-Quan Tang hai-qiang mai Department of Nasopharyngeal Carcinoma State Key Laboratory of Oncology in South ChinaCollaborative Innovation Center for Cancer MedicineGuangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and TherapySun Yat-sen University Cancer CenterGuangzhouGuangdong 510060P.R.China

Background:Capecitabine was previously used as a second-line or salvage therapy for metastatic nasopharyngeal carcinoma(NPC)and has shown satisfactory curative effect as maintenance therapy in other metastatic *** study aimed to explore the role of capecitabine as maintenance therapy in de novo metastatic NPC patients with different plasma Epstein-Barr virus(EBV)DNA levels before ***:We selected de novo metastatic NPC patients treated with locoregional radiotherapy(LRRT)for this retrospective *** propensity score matching(PSM)was applied to balance potential confounders between patients who underwent capecitabine maintenance therapy and those who did not with a ratio of 1:*** survival(OS)was the primary *** association between capecitabine maintenance therapy and survival was assessed using the log-rank test and a Cox proportional hazard ***:Among all patients eligible for this study,64 received capecitabine maintenance therapy after *** PSM,192 patients were identified in the nonmaintenance *** the matched cohort,patients treated with capecitabine achieved a higher 3-year OS rate compared with patients in the non-maintenance group(68.5%vs.61.8%,P=0.037).Multivariate analysis demonstrated that capecitabine maintenance therapy was an independent prognostic *** subgroup analysis,3-year OS rate was comparable between the maintenance and non-maintenance groups in patients with high pretreatment EBV DNA levels(˃30,000 copies/mL)(54.8%vs.45.8%,P=0.835),whereas patients with low pretreatment EBV DNA levels(≤30,000 copies/mL)could benefit from capecitabine maintenance therapy in OS(90.0%vs.68.1%,P=0.003).Conclusion:Capecitabine maintenance therapy may be superior to non-maintenance therapy in prolonging OS for de novo metastatic NPC patients with pretreatment EBV DNA≤30,000 copies/*** in patients with high pretreatment EBV DNA levels(˃30,000 copies/mL)(54.8%vs.45.8%,P=0.835),whereas patients with

关键词： capecitabine de novo Epstein-Barr virus locoregional maintenance therapy nasopharyngeal carcinoma propensity score matching radiotherapy survival

Randomized study of sinusoidal chronomodulated versus flat intermittent induction chemotherapy with cisplatin and 5-fluorouracil followed by traditional radiotherapy for locoregionally advanced nasopharyngeal carcinoma

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Chinese Journal of Cancer 2013年第9期32卷 502-511页

作者： Huan-Xin Lin Yi-Jun Hua Qiu-Yan Chen Dong-Hua Luo Rui Sun Fang Qiu Hao-Yuan Mo hai-qiang mai Xiang Guo Li-Jian Xian Ming-Huang Hong Ling Guo State Key Laboratory of Oncology in South China Department of Radiation Oncology Sun Yat-sen University Cancer Center Department of Nasopharyngeal Cancer Sun Yat-sen University Cancer Center Clinical Research Center Sun Yat-sen University Cancer Center

Neoadjuvant chemotherapy plus radiotherapy is the most common treatment regimen for advanced nasopharyngeal carcinoma(NPC).Whether chronomodulated infusion of chemotherapy can reduce its toxicity is *** study aimed to evaluate the toxic and therapeutic effects of sinusoidal chronomodulated infusion versus flat intermittent infusion of cisplatin(DDP)and 5-fluorouracil(5-FU)followed by radiotherapy in patients with locoregionally advanced *** with biopsy-diagnosed untreated stages III and IV NPC(according to the 2002 UICC staging system)were randomized to undergo2 cycles of sinusoidal chronomodulated infusion(Arm A)or flat intermittent constant rate infusion(Arm B)of DDP and 5-FU followed by radical *** a"MELODIE"multi-channel programmed pump,the patients were given 12-hour continuous infusions of DDP(20 mg/m2)and 5-FU(750 mg/m2)for 5days,repeated every 3 weeks for 2 *** was administered from 10:00 am to 10:00 pm,and 5-FU was administered from 10:00 pm to 10:00 am each *** infusion was performed in Arm A,with the peak deliveries of 5-FU at 4:00 am and DDP at 4:00 *** patients in Arm B underwent a constant rate of *** was initiated in the fifth week,and both arms were treated with the same radiotherapy techniques and dose *** June 2004 and June 2006,125 patients were registered,and 124 were eligible for analysis of response and *** major toxicity observed during neoadjuvant chemotherapy was *** incidence of acute toxicity was similar in both *** radiotherapy,the incidence of stomatitis was significantly lower in Arm A than in Arm B(38.1%vs.59.0%,P=0.020).No significant differences were observed for other *** 1-,3-,and 5-year overall survival rates were 88.9%,82.4%,and 74.8%for Arm A and 91.8%,90.2%,and 82.1%for Arm *** 1-,3-,and 5-year progression-free survival rates were 91.7%,88.1%,and 85.2%for Arm A and 100%,94.5%,and 86.9%for Arm ***

关键词：氟尿嘧啶鼻咽癌放疗化疗顺铂间歇正弦晚期

Clinical findings and imaging features of 67 nasopharyngeal carcinoma patients with postradiation nasopharyngeal necrosis

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Chinese Journal of Cancer 2013年第10期32卷 533-538页

作者： Ming-Yuan Chen hai-qiang mai Rui Sun Xiang Guo Chong Zhao Ming-Huang Hong Yi-Jun Hua State Key Laboratory of Oncology in South China Department of Nasopharyngeal Carcinoma Department of Radiotherapy Sun Yat-sen University Cancer Center

Postradiation nasopharyngeal necrosis is an important late effect of radiotherapy that affects prognosis in patients with nasopharyngeal *** the present study,we reviewed the clinical and imaging features of 67 patients with pathologically diagnosed postradiation nasopharyngeal necrosis who were treated at Sun Yat-sen University Cancer Center between June 2006 and January *** clinical manifestations,endoscopic findings,and imaging features were *** nasopharyngeal necrosis was limited to a local site in the nasopharyngeal region,and the tissue defect was not obvious,whereas deep parapharyngeal ulcer or signs of osteoradionecrosis in the basilar region was observed in serious *** with osteoradionecrosis and/or exposed carotid artery had a high *** conclusion,Postradiation nasopharyngeal necrosis has characteristic magnetic resonance imaging appearances,which associate well with clinical findings,but pathologic examination is essential to make the diagnosis.

关键词：临床表现鼻咽癌影像学特征性坏死放疗患者放射治疗

Ex vivo expansion of tumor-infiltrating lymphocytes from nasopharyngeal carcinoma patients for adoptive immunotherapy

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Chinese Journal of Cancer 2012年第6期31卷 287-294页

作者： Jia He Xiao-Feng Tang Qiu-Yan Chen hai-qiang mai Zhou-Feng Huang Jiang Li Yi-Xin Zeng State Key Laboratory of Oncology in South China GuangzhouGuangdong 510060P.R.China Sun Yat-sen University Cancer Center GuangzhouGuangdong 510060P.R.China Departmentof Biotherapy Sun Yat-sen University Cancer CenterGuangzhouGuangdong 510060P.R.China Department of Nasopharyngeal Carcinoma Sun Yat-sen University Cancer CenterGuangzhouGuangdong 510060P.R.China

Establishing Epstein-Barr virus (EBV)-specific cytolytic T lymphocytes (EBV-CTLs) from peripheral blood mononuclear cells (PBMCs) for adoptive immunotherapy has been reported in EBV-associated malignancies including Hodgkin's lymphoma and nasopharyngeal carcinoma (NPC). In the current study,we performed ex vivo expansion of tumor-infiltrating lymphocytes (TILs) obtained from NPC biopsy specimens with a rapid expansion protocol using anti-CD3 monoclonal antibody (OKT3), recombinant human interleukin (IL)-2, and irradiated PBMCs from healthy donors to initiate the growth of TILs. Young TIL cultures comprised of more than 90% of CD3+T cells, a variable percentage of CD3+CD8+and CD3+CD4+T cells, and less than 10% of CD3-CD16+natural killer cells, a similar phenotype of EBV-CTL cultures from PBMCs. Interestingly, TIL cultures secreted high levels of the Th1 cytokines, interferon gamma (IFNγ) and tumor necrosis factor-alpha (TNF-α), and low levels of the Th2 cytokines, IL-4 and IL-10. Moreover, young TILs could recognize autologous EBV-transformed B lymphoblast cell lines, but not autologous EBV-negative blast cells or allogeneic EBV-negative tumor cells. Taken together, these data suggest that ex vivo expansion of TILs from NPC biopsy tissue is an appealing alternative method to establish T cell-based immunotherapy for NPC.

关键词：细胞毒性T淋巴细胞体外扩增免疫治疗恶性肿瘤鼻咽癌 Epstein-Barr病毒外周血单个核细胞浸润性

Elevated peripheral blood lymphocyteto-monocyte ratio predicts a favorable prognosis in the patients with metastatic nasopharyngeal carcinoma

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癌症 2015年第6期34卷 237-246页

作者： Rou Jiang Xiu-Yu Cai Zhong-Han Yang Yue Yan Xiong Zou Ling Guo Rui Sun Dong-Hua Luo Qiu-Yan Chen Pei-Yu Huang Yan-Qun Xiang Xing Lu Lin Wang Wei-Xiong Xia hai-qiang mai Ming-Yuan Chen Sun Yat-sen University Cancer Center State Key Laboratory of Oncology in South China Collaborative Innovation Center for Cancer Medicine 4. Department of Nasopharyngeal Carcinoma Sun Yat-sen University Cancer Center 5. Department of Cancer Prevention 6. Department of Medical Oncology Sun Yat-sen University Cancer Center Department of Biochemistry Zhongshan School of MedicineSun Yat-sen University

Introduction:Patients with metastatic nasopharyngeal carcinoma(NPC)have variable survival *** have previously shown that an elevated peripheral blood lymphocyte-to-monocyte ratio(LMR)is associated with an increased metastatic risk in patients with primary *** present study aimed to investigate the prognostic value of pretreatment LMR in a large cohort of metastatic NPC ***:Clinical data of 672 patients with metastatic NPC diagnosed between January 2003 and December2009 were *** peripheral lymphocyte and monocyte counts were retrieved,and LMR was *** operating characteristic(ROC)curve analysis and univariate and multivariate COX proportional hazards analyses were performed to evaluate the association of LMR with overall survival(OS).Results:Univariate analysis revealed that an elevated absolute lymphocyte count(≥1.390×109/L)and LMR(≥2.475)as well as a decreased monocyte count(mained a significant predictor of ***:We identified pretreatment LMR as an independent prognostic factor for patients with metastatic *** validation of our findings is needed.

关键词： Metastatic nasopharyngeal carcinoma Lymphocyte count Monocyte count Lymphocyte-to-monocyte ratio Overall survival Prognosis

Locoregional radiotherapy in patients with distant metastases of nasopharyngeal carcinoma at diagnosis

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Chinese Journal of Cancer 2013年第11期32卷 604-613页

作者： Ming-Yuan Chen Rou Jiang Ling Guo Xiong Zou Qing Liu Rui Sun Fang Qiu Zhong-Jun Xia Hui-qiang Huang Li Zhang Ming-Huang Hong hai-qiang mai Chao-Nan Qian Sun Yat-sen University Cancer Center State Key Laboratory of Oncology in South China Collaborative Innovation Center of Cancer Medicine Department of Nasopharyngeal Carcinoma Sun Yat-sen University Cancer Center Department of Epidemiology Sun Yat-sen University Cancer Center Department of Medical Oncology Sun Yat-sen University Cancer Center

Systemic chemotherapy is the basic palliative treatment for metastatic nasopharyngeal carcinoma(NPC); however, it is not known whether locoregional radiotherapy targeting the primary tumor and regional lymph nodes affects the survival of patients with metastatic NPC. Therefore, we aimed to retrospectively evaluate the benefits of locoregional radiotherapy. A total of 408 patients with metastatic NPC were included in this study. The mortality risks of the patients undergoing supportive treatment and those undergoing chemotherapy were compared with that of patients undergoing locoregional radiotherapy delivered alone or in combination with chemotherapy. Univariate and multivariate analyses were conducted. The contributions of independent factors were assessed after adjustment for covariates with significant prognostic associations (P<0.05). Both locoregional radiotherapy and systemic chemotherapy were identified as significant independent prognostic factors of overall survival(OS). The mortality risk was similar in the group undergoing locoregional radiotherapy alone and the group undergoing systemic chemotherapy alone [multi-adjusted hazard ratio(HR)=0.9, P=0.529]; this risk was 60% lower than that of the group undergoing supportive treatment(HR=0.4, P=0.004) and 130% higher than that of the group undergoing both systemic chemotherapy and locoregional radiotherapy(HR=2.3, P<0.001). In conclusion, locoregional radiotherapy, particularly when combined with systemic chemotherapy, is associated with improved survival of patients with metastatic NPC.

关键词：鼻咽癌放疗患者放射治疗诊断多因素分析转移性化疗

A new prognostic histopathologic classification of nasopharyngeal carcinoma

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Chinese Journal of Cancer 2016年第6期35卷 30-45页

作者： hai-Yun Wang Yih-Leong Chang Ka-Fai To Jacqueline S.G.Hwang hai-qiang mai Yan-Fen Feng Ellen T.Chang Chen-Ping Wang Michael Koon Ming Kam Shie-Lee Cheah Ming Lee Li Gao Hui-Zhong Zhang Jie-Hua He Hao Jiang Pei-Qing Ma Xiao-Dong Zhu Liang Zeng Chun-Yan Chen Gang Chen Ma-Yan Huang Sha Fu Qiong Shao An-Jia Han hai-Gang Li Chun-Kui Shao Pei-Yu Huang Chao-Nan Qian Tai-Xiang Lu Jin-Tian Li Weimin Ye Ingemar Ernberg Ho Keung Ng Joseph T.S.Wee Yi-Xin Zeng Hans-Olov Adami Anthony T.C.Chan Jian-Yong Shao State Key Laboratory of Oncology in South China Collaborative Innovation Center for Cancer Medicine Sun Yat-sen University Cancer Center Department of Molecular Diagnostics Sun Yat-sen University Cancer Center Department of Pathology National Taiwan University HospitalNational Taiwan University College of Medicine Department of Anatomical and Cellular Pathology Hong Kong Cancer Institute and Sir YK Pao Centre for CancerFaculty of MedicineThe Chinese University of Hong Kong Department of Pathology Singapore General Hospital Department of Nasopharyngeal Carcinoma Sun Yat-sen University Cancer Center Department of Pathology Sun Yat-sen University Cancer Center Division of Epidemiology Department of Health Research and PolicyStanford University School of Medicine Health Sciences Practice ExponentInc. Department of Otolaryngology National Taiwan University HospitalNational Taiwan University College of Medicine Department of Clinical Oncology Hong Kong Cancer Institute and Sir YK Pao Centre for CancerFaculty of MedicineThe Chinese University of Hong Kong Division of Radiation Oncology National Cancer CenterSingapore Department of Radiation Oncology Cancer Institute and HospitalChinese Academy of Medical Sciences Department of Radiation Oncology The Affiliated Hospital of Bengbu Medical College Department of Pathology Cancer Institute and HospitalChinese Academy of Medical Sciences Department of Radiation Oncology Guangxi Medical University Department of Pathology Hunan Provincial Cancer Hospital Department of Radiation Oncology Sun Yat-sen University Cancer Center Department of Pathology Fujian Provincial Cancer Hospital Department of Pathology The First Affiliated HospitalSun Yat-sen University Department of Pathology Sun Yat-sen Memorial HospitalSun Yat-sen University Department of Pathology The Third Affiliated HospitalSun Yat-sen University Department of Experiment Research Sun Yat-sen University Cancer Center Department of Medical Epidemiology and Biostatistics Karolinska Institutet Departments of Mi

Background:The current World Health Organization(WHO) classification of nasopharyngeal carcinoma(NPC) con?veys little prognostic *** study aimed to propose an NPC histopathologic classification that can poten?tially be used to predict prognosis and treatment ***:We initially developed a histopathologic classification based on the morphologic traits and cell differentia?tion of tumors of 2716 NPC patients who were identified at Sun Yat?sen University Cancer Center(SYSUCC)(training cohort).Then,the proposed classification was applied to 1702 patients(retrospective validation cohort) from hospitals outside SYSUCC and 1613 patients(prospective validation cohort) from *** efficacy of radiochemotherapy and radiotherapy modalities was compared between the proposed *** used Cox proportional hazards models to estimate hazard ratios(HRs) with 95% confidence intervals(CI) for overall survival(OS).Results:The 5?year OS rates for all NPC patients who were diagnosed with epithelial carcinoma(EC;3708 patients),mixed sarcomatoid?epithelial carcinoma(MSEC;1247 patients),sarcomatoid carcinoma(SC;823 patients),and squamous cell carcinoma(SCC;253 patients) were 79.4%,70.5%,59.6%,and 42.6%,respectively(P < 0.001).In mul?tivariate models,patients with MSEC had a shorter OS than patients with EC(HR = 1.44,95% CI = 1.27–1.62),SC(HR = 2.00,95% CI = 1.76–2.28),or SCC(HR = 4.23,95% CI = 3.34–5.38).Radiochemotherapy significantly improved survival compared with radiotherapy alone for patients with EC(HR 49–0.75),and possibly for those with SCC(HR = 0.67,95% CI = 0.56–0.80),MSEC(HR = 0.58,95% CI = 0..74–1.28).= 0.63;95% CI = 0.40–0.98),but not for patients with SC(HR = 0.97,95% CI = 0Conclusions:The proposed classification offers more information for the prediction of NPC prognosis compared with the WHO classification and might be a valuable tool to guide treatment decisions for subtypes that are associ?ated with a poor prognosis.

关键词： Nasopharyngeal carcinoma Pathologic classification Prognosis

A curative-intent endoscopic surgery for postradiation nasopharyngeal necrosis in patients with nasopharyngeal carcinoma

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Cancer Communications 2018年第1期38卷 783-793页

作者： Xiong Zou Shun-Lan Wang You-Ping Liu Yan-Ling Liu Ru-hai Zou Yi-Nuan Zhang Rui You Qi Yang Yu-Long Xie Mei Lin Pei-Yu Huang Rou Jiang Meng-Xia Zhang Chao-Nan Qian hai-qiang mai Ling Guo Ming-Huang Hong Ming-Yuan Chen State Key Laboratory of Oncology in South China Collaborative Innovation Center for Cancer MedicineSun Yat-sen University Cancer CenterGuangzhou 510060GuangdongP.R.China Department of Nasopharyngeal Carcinoma Sun Yat-sen University Cancer Center651 Dongfeng Road EastGuangzhou 510060GuangdongP.R.China Guangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and Therapy Sun Yat-sen University Cancer CenterGuangzhou 510060GuangdongP.R.China Department of Otorhinolaryngology First Hospital Affiliated of Guangzhou University of Traditional Chinese MedicineGuangzhou 510405GuangdongP.R.China Department of Ultrasound Sun Yat-sen University Cancer CenterGuangzhou 510060GuangdongP.R.China

Background:Postradiation nasopharyngeal necrosis(PRNN)is a severe complication after radiotherapy in patients with nasopharyngeal carcinoma(NPC),which can severely affect the quality of life and threaten the patient’s *** 13.4%-28.6%of patients can be cured by traditional repeated endoscopic ***,we introduced an innovative curative-intent endoscopic surgery for PRNN patients and evaluated its clinical ***:Clinical data of 72 PRNN patients who underwent radical endoscopic necrectomy,followed by reconstruc-tion using a posterior pedicle nasal septum and floor mucoperiosteum flap were analyzed to determine the efficacy of this *** endpoints were complete re-epithelialization of the nasopharyngeal defect,relief of headache,and overall survival(OS).Results:All surgeries were successfully performed without any severe postoperative complications or *** median value of numeric rating scales of pain decreased from 8 before surgery to 0 after surgery(P<0.001).Fifty-one patients(70.8%)achieved complete re-epithelialization of the nasopharyngeal *** number of cycles of radiotherapy(odds ratio[OR],7.254;95%confidence interval[CI]1.035-50.821;P=0.046),postoperative pathological result(OR,34.087;95%CI 3.168-366.746;P=0.004),and survival status of flap(OR,261.179;95%CI 17.176-3971.599;P<0.001)were independent risk factors of re-epithelialization of the nasopharyngeal *** patho-logical result(hazard ratio[HR],5.018;95%CI 1.970-12.782;P=0.001)was an independent prognostic factor for *** 2-year OS rate of the entire cohort was 77.9%.Conclusion:Curative-intent endoscopic necrectomy followed by construction using the posterior pedicle nasal septum and floor mucoperiosteum flap is a novel,safe,and effective treatment of PRNN in patients with NPC.

关键词： Nasopharyngeal carcinoma Postradiation Necrosis Endoscopy Necrectomy Flap Reconstruction Re-epithelialization

鼻咽癌细胞株中C-KIT表达和突变以及对Imatinib的反应性

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癌症 2010年第2期29卷 137-142页

作者：黄培钰洪明晃张星麦海强罗东华张力华南肿瘤学国家重点实验室广东广州510060 中山大学肿瘤防治中心鼻咽癌科广东广州510060 中山大学肿瘤防治中心生物治疗中心广东广州510060 中山大学肿瘤防治中心内科广东广州510060

背景与目的:我们先前报道了鼻咽癌组织中存在C-KIT表达和突变,但体外水平Imatinib是否对鼻咽癌细胞有增殖抑制作用及其机制尚不清楚。本研究评价了鼻咽癌细胞株中C-KIT表达和突变以及对Imatinib的反应性,探讨三者之间的相关性。方法:应... 详细信息

背景与目的:我们先前报道了鼻咽癌组织中存在C-KIT表达和突变,但体外水平Imatinib是否对鼻咽癌细胞有增殖抑制作用及其机制尚不清楚。本研究评价了鼻咽癌细胞株中C-KIT表达和突变以及对Imatinib的反应性,探讨三者之间的相关性。方法:应用Western blot检测鼻咽癌细胞株CNE-1、CNE-2、Hone-1、C-666、SUNE-1、5-8F及鼻咽上皮细胞株NP-69的C-KIT表达情况,应用直接测序法检测CNE-1、CNE-2、Hone-1、C-666、SUNE-1、5-8F、NP-69的C-KIT基因突变情况,应用CCK-8试剂盒进行Imatinib对CNE-1、CNE-2、Hone-1、C-666、SUNE-1及5-8F的增殖抑制实验,应用Pearson相关分析和t检验分析鼻咽癌细胞株中C-KIT表达、突变和对Imatinib的反应性三者之间是否有相关性。结果:鼻咽癌CNE-1、CNE-2、Hone-1、C-666、SUNE-1及5-8F细胞株相对于鼻咽上皮细胞株NP-69存在C-KIT蛋白高表达。CNE-1、CNE-2、Hone-1、NP-69发现杂合IVS17+78T>C,C-666发现纯合IVS17+78T>C,SUNE-1及5-8F无突变。Imatinib对CNE-1、CNE-2、Hone-1、C-666、SUNE-1及5-8F细胞株有剂量依赖的增殖抑制作用。鼻咽癌细胞株中C-KIT表达、突变和对Imatinib的反应性三者之间的相关性无统计学意义。结论:鼻咽癌细胞株中存在C-KIT过表达和C-KIT内含子基因突变,Imatinib对鼻咽癌细胞株有剂量依赖的增殖抑制作用,但是C-KIT蛋白过表达、C-KIT突变和Imatinib的增殖抑制作用之间未发现明显的相关性。

关键词：鼻咽肿瘤 C-KIT 蛋白表达基因突变增殖抑制作用