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Fingerprinting Analysis of Rhizoma Chuanxiong of Commercial Types using ~1H Nuclear Magnetic Resonance Spectroscopy and High Performance Liquid Chromatography Method

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Journal of Integrative Plant Biology 2009年第6期51卷 537-544页

作者： hai-Lin Qin An-jun deng Guan-Hua Du Peng Wang Jin-Lan Zhang Zhi-Hong Li Institute of Materia Medica Chinese Academy of Medical Sciences Peking Union Medical College Key Laboratory of Bioactive Substances and Resources of Chinese Herbal Medicine Ministry of Education Beijing 100050 China

The ^1 H nuclear magnetic resonance （^1 H NMR） fingerprints of fractionated non-polar extracts （control substance for a plant drug （CSPD） A） from Rhizoma chuanxiong, the rhizomes of Ligusticum chuanxiong Hort., of seven specimens from different sources were measured on Fourier Transform （FT）-NMR spectrometer and assigned by comparing them with the ^1 H NMR spectra of the isolated pure compounds. The ^1 H NMR fingerprints showed exclusively characteristic resonance signals of the major special constituents of the plant. Although the differences in the relative intensity of the ^1H NMR signals due to a discrepancy in the ratio of the major constituents among these samples could be confirmed by high performance liquid chromatography analysis, the general features of the ^1H NMR fingerprint established for an authentic sample of the rhizomes of L. chuanxiong e. hibited exclusive data from those special compounds and can be used for authenticating L. Chuanxiong species.

关键词： control substance for a plant drug A ^1 H nuclear magnetic resonance fingerprint high performance liquid chromatography Ligusticumchuanxiong phenylpropenoid phthalides.

miRNA-338-3p suppresses cell growth of human colorectal carcinoma by targeting smoothened

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World Journal of Gastroenterology 2013年第14期19卷 2197-2207页

作者： Kai Sun hai-jun deng Shang-Tong Lei Jing-Qing Dong Guo-Xin Li Department of General Surgery Nanfang Hospital of Southern Medical University

AIM:To investigate the regulative effect of miRNA-3383p(miR-338-3p) on cell growth in colorectal carcinoma(CRC).METHODS:The lentiviral vector pLV-THM-miR-338-3p and pLV-THM-miR-338-3p-inhibitor were *** recombinant viral vector encoding the pre-miR338-3p or miR-338-3p-inhibitor and the two packaging plasmids psPAX2 and pMD2.G were cotransfected into human embryonic kidney 293T cells to package *** supernatant containing the lentivirus particles was harvested to determine the viral titer,and this supernatant was then used to transduce CRCderived cell line,*** cytometry was utilized for sorting the green fluorescent protein(GFP) + cells to establish the SW-620 cell line stably expressing premiR-338-3p or ***,the expression of miR-338-3p was determined by real-time reverse transcriptase polymerase chain reaction,andWestern blotting was used to detect the expression of the smoothened(SMO,the possible target of miR-3383p) protein in SW-620 ***,the status of CRC cell proliferation and apoptosis were detected by 3-(4,5-dimethyl-2 thiazoyl)-2,5-diphenyl-2H-tetrazolium bromide assay and flow cytometry,***:Restriction enzyme digestion and DNA sequencing demonstrated that the lentiviral vector pLVTHM-miR-338-3p and pLV-THM-miR-338-3p-inhibitor were constructed *** was expressed after the SW-620 cells were transduced by the *** of miR-338-3p in SW-620 cells transduced with the lentivirus pLV-THM-miR-338-3p was significantly increased(relative expression 3.91 ± 0.51 vs 2.36 ± 0.44,P < 0.01).Furthermore,overexpression of miR-338-3p inhibited the expression of SMO protein in SW-620 cells,which showed obviously suppressed proliferation ability [cellular proliferation inhibition rate(CPIR) 61.9% ± 5.2% vs 41.6% ± 4.8%,P < 0.01].Expression of miR-338-3p in SW-620 cells transduced with the lentivirus pLV-THM-miR-338-3p-inhibitor was significantly decreased(relative ex

关键词： Colorectal carcinoma Hsa-miRNA-338-3p Smoothened Lentivirus

Anti-miRNA-221 sensitizes human colorectal carcinoma cells to radiation by upregulating PTEN

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World Journal of Gastroenterology 2013年第48期19卷 9307-9317页

作者： Qi Xue Kai Sun hai-jun deng Shang-Tong Lei Jing-Qing Dong Guo-Xin Li Department of General Surgery Nanfang Hospital of Southern Medical University

AIM:To investigate the regulative effect of miRNA(miR)-221 on colorectal carcinoma(CRC)cell radiosensitivity and the underlying ***:A human CRC-derived cell line was cultured conventionally and exposed to different doses of X-rays(0,2,4,6 and 8 Gy).The total RNA and protein of the cells were extracted 24 h after irradiation,and the alteration of miR-221 and phosphatase and tensin homolog deleted on chromosome 10(PTEN)gene mRNA expression was detected by real-time reverse transcriptase polymerase chain reaction(PCR).The protein alteration of PTEN in the cells was detected by Western ***2 cells were pretreated with or without anti-PTEN-siRNA prior to the addition of premiR-221 or anti-miR-221 using Lipofectamine *** formation assay and flow cytometry analysis were used to measure the surviving cell fraction and the sensitizing enhancement ratio after ***-ditionally,PTEN 3′-untranslated region fragment was PCR amplified and inserted into a luciferase reporter *** luciferase reporter plasmid construct was then transfected into CRC cells together with premiR-221 or anti-miR-221,and the luciferase activity in the transfected cells was ***:The X-ray radiation dose had a significant effect on the expression of miR-221 and PTEN protein in human Caco2 cells in a dose-dependent *** miR-221 expression level improved gradually with the increase in irradiation dose,while the PTEN protein expression level reduced ***-221 expression was significantly reduced in the anti-miR-221 group compared with the pre-miR-221 and negative control groups(P<0.01).Anti-miR-221 upregulated expression of PTEN protein and enhanced the radiosensitivity of Caco2 cells(P<0.01).Moreover,the inhibitory effect was dramatically abolished by pretreatment with anti-PTEN-siRNA,suggesting that the enhancement of radiosensitivity was indeed mediated by PTEN.A significant increase of luciferase activity was detected in CRC cells tha

关键词： Colorectal carcinoma miR-221 Phosphatase and tensin homolog deleted on chromosome 10 Radiosensitivity

Techniques and feasibility of laparoscopic extended right hemicolectomy with D3 lymphadenectomy

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World Journal of Gastroenterology 2014年第30期20卷 10531-10536页

作者： Li-Ying Zhao Hao Liu Ya-Nan Wang hai-jun deng Qi Xue Guo-Xin Li Department of General Surgery Nanfang HospitalSouthern Medical University

AIM: To illustrate the critical techniques and feasibility of laparoscopic extended right hemicolectomy (LERH), according to our previous experience.

关键词： Colon cancer Hepatic flexure Laparoscopic colectomy D3 lymphadenectomy Anatomy

Pharmacokinetics and tissue distribution of intraperitoneal 5-fluorouracil with a novel carrier solution in rats

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World Journal of Gastroenterology 2008年第14期14卷 2179-2186页

作者： Zhi-Gang Wei Guo-Xin Li Xiang-Cheng Huang Li Zhen Jiang Yu hai-jun deng Shan-Hua Qing Ce Zhang Department of General Surgery Nanfang Hospital Southern Medical University Guangzhou 510515 Guangdong Province China

AIM: To compare the pharmacokinetics and tissue distribution of 5-fluorouracil administered intraperitoneally with two isotonic carrier solutions: HAES-steri (neotype 6% hydroxyethyl starch), a novel carrier solution with middle molecular weight and physiologic saline (0.9% sodium chloride solution), a traditional carrier solution for intraperitoneal chemotherapy, in rats. METHODS: A total of 60 Sprague Dawley rats were randomized into groups according to the carrier solution administered. Each group was further randomized according to the intraperitoneal dwell period (1, 3, 6, 12, 18 and 24 h). At the end of the procedure the rats were killed, the peritoneal fluid was withdrawn completely and quantitated. Drug concentrations in peritoneal fluid, plasma, and tissues were determined by high- performance liquid chromatography. RESULTS: The mean volumes remaining in the peritoneal cavity were significantly higher with HAES- steri than those with physiologic saline at 1, 6, 12, 18, and 24 h (P = 0.047, 0.009, 0.005, 0.005 and 0.005 respectively, the percentages of remaining peritoneal fluid volume were 89.9 ± 5.6 vs 83.4 ± 4.9, 79.9 ± 2.8 vs 56.2 ± 15.7, 46.8 ± 5.5 vs 24.7 ± 9.7, 23.0 ± 2.8 vs 0.0 ± 0.0 and 4.2 ± 1.7 vs 0.0 ± 0.0 respectively). Mean concentrations in peritoneal fluid were significantly higher with HAES-steri than those with physiologic saline at 3, 12 and 18 h (P = 0.009, 0.009 and 0.005 respectively, the concentrations were 139.2768 ± 28.2317 mg/L vs mg/L, 11.5427 ± 3.0976 mg/L vs 0.0000 ± 0.0000 mg/L and 4.7724 ± 1.0936 mg/L vs 0.0000 ± 0.0000 mg/L respectively). Mean plasma 5-fluorouracil concentrations in portal vein were significantly higher with HAES-steri at 3, 12, 18 and 24 h (P = 0.009, 0.034, 0.005 and 0.019 respectively, the concentrations were 3.3572 ± 0.8128 mg/L vs 0.8794 ± 0.2394 mg/L, 0.6203 ± 0.9935 mg/L vs 0.0112 ± 0.0250 mg/L, 0.3725 ± 0.3871 mg/L vs 0.0000 ± 0.0000 mg/L, and 0.2469 ± 0.1457 mg/L vs 0.0000 ± 0.0000 mg/L respectively),

关键词： Carrier solutions Intraperitoneal chemo- therapy 5-fluorouracil Pharmacokinetics Tissue distribution

Changes in the humoral immunity response in SARS-CoV-2 convalescent patients over 8 months

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Cellular & Molecular Immunology 2021年第2期18卷 490-491页

作者： Pai Peng Jie Hu hai-jun deng Bei-zhong Liu Liang Fang Kai Wang Ni Tang Ai-long Huang Key Laboratory of Molecular Biology for Infectious Diseases(Ministry of Education) Institute for Viral HepatitisDepartment of Infectious DiseasesThe Second Affiliated HospitalChongqing Medical UniversityChongqingChina Yong-Chuan Hospital Chongqing Medical UniversityChongqing402160China

Many countries around the world have seen a sharp rise in COVID-19 cases since the beginning of October due to the second wave of the pandemic.A decline in the antibody response to severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),which was reported exclusively in the early month,increases the risk of reinfection for convalescent *** is a current need to follow the maintenance of specific antibodies against SARS-CoV-2.

关键词： immunity acute respiratory

Real-time in vivo distal margin selection using confocal laser endomicroscopy in transanal total mesorectal excision for rectal cancer

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World Journal of Gastrointestinal Surgery 2022年第12期14卷 1375-1386页

作者： Jie Tan Hong-Li Ji Yao-Wen Hu Zhi-Ming Li Bao-Xiong Zhuang hai-jun deng Ya-Nan Wang Ji-XiangZheng Wei Jiang jun Yan Department of General Surgery Nanfang HospitalGuangzhou 510515Guangdong ProvinceChina Department of General Surgery Southern Medical UniversityGuangzhou 510515Guangdong ProvinceChina Guangdong Provincial Key Laboratory of Precision Medicine for Gastrointestinal Tumor Nanfang HospitalGuangzhou 510515Guangdong ProvinceChina

BACKGROUND Transanal total mesorectal excision(TaTME)allows patients with ultralow rectal cancer to be treated with sphincter-saving ***,accurate delineation of the distal resection margin(DRM),which is essential to achieve R0 resection for low rectal cancer in TaTME,is technically *** To assess the feasibility of optical biopsy using probe-based confocal laser endomicroscopy(pCLE)to select the DRM during TaTME for low rectal *** A total of 43 consecutive patients who were diagnosed with low rectal cancer and scheduled for TaTME were prospectively enrolled from January 2019 to January *** was used to determine the distal edge of the tumor as well as the DRM during *** final pathological report was used as the gold *** diagnostic accuracy of pCLE examination was *** A total of 86 pCLE videos of 43 patients were included in the *** sensitivity,specificity and accuracy of real-time pCLE examination were 90.00%[95%confidence interval(CI):76.34%-97.21%],86.96%(95%CI:73.74%-95.06%)and 88.37%(95%CI:79.65%-94.28%),*** accuracy of blinded pCLE reinterpretation was 86.05%(95%CI:76.89%-92.58%).Furthermore,our results show satisfactory interobserver agreement(κ=0.767,standard error=0.069)for the detection of cancer tissue by *** were no positive DRMs(≤1 mm)in this *** median DRM was 7 mm[interquartile range(IQR)=5-10 mm].The median Wexner score was 5(IQR=3-6)at 6 mo after stoma *** Real-time in vivo pCLE examination is feasible and safe for selecting the DRM during TaTME for low rectal cancer(clinical trial registration number:NCT04016948).

关键词： Transanal total mesorectal excision Probe-based confocal laser endomicroscopy Optical biopsy Distal resection margin Low rectal cancer

Survival outcomes following laparoscopic vs open surgery for non-metastatic rectal cancer:a two-center cohort study with propensity score matching

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Gastroenterology Report 2020年第4期8卷 319-325,I0002,I0003页

作者： Kang-Lian Tan hai-jun deng Zhi-Qiang Chen Ting-Yu Mou Hao Liu Run-Sheng Xie Xue-Min Liang Xiao-Hua Fan Guo-Xin Li Department of General Surgery Nanfang HospitalSouthern Medical UniversityGuangzhouGuangdongP.R.China Abdominal Pain Center Guangdong Provincial Hospital of Chinese MedicineThe Second Affiliated Hospital of Guangzhou University of Chinese MedicineGuangzhouGuangdongP.R.China

Background:Laparoscopic surgery for rectal cancer is commonly performed in ***,compared with open surgery,the effectiveness of laparoscopic surgery,especially the long-term survival,has not been sufficiently ***:Data of eligible patients with non-metastatic rectal cancer at Nanfang Hospital of Southern Medical University and Guangdong Provincial Hospital of Chinese Medicine between 2012 and 2014 were retrospectively ***-termsurvival outcomes and short-termsurgical safety were analysed with propensity score matching between ***:Of 430 cases collated from two institutes,103 matched pairs were analysed after propensity score *** estimated blood loss during laparoscopic surgerywas significantly less than that during open surgery(P=0.019)and the operative time and hospital staywere shorter in the laparoscopic group(both P<0.001).The post-operative complications ratewas 9.7%in the laparoscopic group and 10.7%in the open group(P=0.818).No significant differencewas observed between the laparoscopic group and the open group in the 5-year overall survival rate(75.7%vs 80.6%,P=0.346),5-year relapse-free survival rate(74.8%vs 76.7%,P=0.527),or 5-year cancer-specific survival rate(79.6%vs 87.4%,P=0.219).An elevated carcinoembryonic antigen,<12 harvested lymph nodes,and perineural invasion were independent prognostic factors affecting overall survival and relapse-free ***:Our findings suggest that open surgery should still be the priority recommendation,but laparoscopic surgery is also an acceptable treatment for non-metastatic rectal cancer.

关键词： laparoscopic surgery open surgery propensity score matching rectal cancer

Correction: Changes in the humoral immunity response in SARS-CoV-2 convalescent patients over 8 months

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Cellular & Molecular Immunology 2022年第12期19卷 1440-1440页

作者： Pai Peng Jie Hu hai-jun deng Bei-zhong Liu Liang Fang Kai Wang Ni Tang Ai-long Huang Key Laboratory of Molecular Biology for Infectious Diseases (Ministry of Education) Institute for Viral Hepatitis Department of Infectious Diseases The Second Affiliated Hospital Chongqing Medical University Chongqing China Yong-Chuan Hospital Chongqing Medical University Chongqing 402160 China

Correction to:Cellular&Molecular Immunology https:/***/10.1038/s41423-020-00605-4,published online 08 January 2021 The licence information was missing from this article and should havebeen *** original article has bee... 详细信息

关键词： immunity Immunology patients