Anti-miRNA-221 sensitizes human colorectal carcinoma cells to radiation by upregulating PTEN
Anti-miRNA-221 sensitizes human colorectal carcinoma cells to radiation by upregulating PTEN作者机构：Department of General Surgery Nanfang Hospital of Southern Medical University
出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)
年 卷 期：2013年第19卷第48期
页 面：9307-9317页
核心收录：
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：Supported by National Natural Science Foundation of China,No.81101896 the National Research Foundation for Doctoral Program of Higher Education of China,No.20124433110010
主 题：Colorectal carcinoma miR-221 Phosphatase and tensin homolog deleted on chromosome 10 Radiosensitivity
摘 要：AIM:To investigate the regulative effect of miRNA(miR)-221 on colorectal carcinoma(CRC)cell radiosensitivity and the underlying ***:A human CRC-derived cell line was cultured conventionally and exposed to different doses of X-rays(0,2,4,6 and 8 Gy).The total RNA and protein of the cells were extracted 24 h after irradiation,and the alteration of miR-221 and phosphatase and tensin homolog deleted on chromosome 10(PTEN)gene mRNA expression was detected by real-time reverse transcriptase polymerase chain reaction(PCR).The protein alteration of PTEN in the cells was detected by Western ***2 cells were pretreated with or without anti-PTEN-siRNA prior to the addition of premiR-221 or anti-miR-221 using Lipofectamine *** formation assay and flow cytometry analysis were used to measure the surviving cell fraction and the sensitizing enhancement ratio after ***-ditionally,PTEN 3′-untranslated region fragment was PCR amplified and inserted into a luciferase reporter *** luciferase reporter plasmid construct was then transfected into CRC cells together with premiR-221 or anti-miR-221,and the luciferase activity in the transfected cells was ***:The X-ray radiation dose had a significant effect on the expression of miR-221 and PTEN protein in human Caco2 cells in a dose-dependent *** miR-221 expression level improved gradually with the increase in irradiation dose,while the PTEN protein expression level reduced ***-221 expression was significantly reduced in the anti-miR-221 group compared with the pre-miR-221 and negative control groups(P0.01).Anti-miR-221 upregulated expression of PTEN protein and enhanced the radiosensitivity of Caco2 cells(P0.01).Moreover,the inhibitory effect was dramatically abolished by pretreatment with anti-PTEN-siRNA,suggesting that the enhancement of radiosensitivity was indeed mediated by PTEN.A significant increase of luciferase activity was detected in CRC cells tha
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