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Chinese Chemical Letters 2010年第6期21卷 633-636页

作者： hai tao xi Tian Tian Yi xiao Qiang Sun Key Laboratory of Fine Petrochemical Engineering Jiangsu Polytechnic UniversityChangzhou 213164China

Two novel fluorine-containing cyclophanes 1 and 2 incorporating a 1,4-bismethylfluorophenylene subunits have been synthesized via the template-directed clipping methodology and their structure was characterized by ~1H NMR,^(13)C NMR, MALDI-TOF-MS and elemental analysis.X-ray diffraction analysis of the solid state molecular structure showed that the complex units are linked by non-classical C-H…F hydrogen bonding.

关键词： Fluorine-cotiaining cyclophanes 1,4-Bis(bromomethyl)-2-fluorobenzene Crystal structure Synthesis

Eukaryotic expression and biological activity analysis of neuroprotective peptide [Gly14]-Humanin

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Journal of Pharmaceutical Analysis 2010年第2期22卷 105-110页

作者： Hui Jin,hai-tao Hu ,Wei-xi Wang,Gai-Feng Feng,Zhao-Hui Liu,Wei-Na YangDepartment of Human Anatomy and Histoembryology,Medical School of xi’an Jiaotong University,xi’an 710061,China. Department of Human Anatomy and Histoembryology Medical School of Xi'an Jiaotong University Xi'an 710061 China

Objective To investigate the expression of neuroprotective peptide [Gly14]-Humanin (HNG) in eukaryotic cells by gene engineering technique and analyze its biological activity. Methods By means of asymmetrical primer/template,double stranded cDNA of HNG with FLAG in its C-terminal was obtained,which was cloned into the plasmid pcDNA3.1(-),and the resultant recombinant vector pcDNA3.1(-)/HNG-FLAG was transfected into PC12 cells. At the same time,the recombinant vector pcDNA3.1(-)/EGFP was transfected to control the efficiency of transfection. The expression of HNG in the cells was determined by immunocytochemistry. In order to analyze the biological activity of the expressed HNG,25μM Aβ25-35 peptide was added to the culture medium of the transfected cells for 24h,then cell morphology,MTT assay and Hoechst 33258 staining were observed. Results The eukaryotic expression vector of pcDNA3.1(-)/HNG-FLAG was identified by enzyme digestion and sequencing. HNG was highly expressed in PC12 cells. After exposure of PC12 cells to 25μM Aβ25-35 for 24h,cell viability decreased to (65.8±5.3)%,and the dystrophic changes of neuritis and nuclei condensation were obvious. When cells were pre-transfected with pcDNA3.1(-)/HNG-FLAG,Aβ25-35-induced cell death and morphological changes of cells and nuclei were suppressed. In contrast,pre-transfected with empty vector did not protect cells from Aβ25-35-induced toxicity. Conclusion The eukaryotic expression vector for FLAG-tagged HNG was successfully constructed and expressed in PC12 cells. Expressed HNG has biological activity.

关键词： Alzheimer's disease [Gly14]-Humanin eukaryotic expression biological activity

Small molecule proteomics quantifies differences between normal and fibrotic pulmonary extracellular matrices

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Chinese Medical Journal 2020年第10期133卷 1192-1202页

作者： xin-Long Wan Zhi-Liang Zhou Peng Wang xiao-Ming Zhou Meng-Ying xie Jin Mei Jie Weng hai-tao xi Chan Chen Zhi-Yi Wang Zhi-Bin Wang Platform for Radiation Protection and Emergency Preparedness of Southern Zhejiang School of Public Health and ManagementWenzhou Medical UniversityWenzhouZhejiang 325035China Center for Health Assessment Wenzhou Medical UniversityWenzhouZhejiang 325035China Department of Emergency Medicine and General Practice The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical UniversityWenzhouZhejiang 325027China Department of Geriatric Medicine The First Affiliated Hospital of Wenzhou Medical UniversityWenzhouZhejiang 325000China lnstitute of Bioscaffold Transplantation and Immunology School of Basic Medical SciencesWenzhou Medical UniversityWenzhouZhejiang 325035China.

Background:Pulmonary fibrosis is a respiratory disease caused by the proliferation of fibroblasts and accumulation of the extracellular matrix(ECM).It is known that the lung ECM is mainly composed of a three-dimensional fiber mesh filled with various high-molecular-weight ***,the small-molecular-weight proteins in the lung ECM and their differences between normal and fibrotic lung ECM are largely ***::Healthy adult male Sprague-Dawley rats(Rattus norvegicus)weighing about 150 to 200 g were randomly divided into three groups using random number table:A,B,and C and each group contained five *** rats in Group A were administered a single intragastric(i.g.)dose of 500μL of saline as control,and those in Groups B and C were administered a single *** of paraquat(PQ)dissolved in 500μL of saline(20 mg/kg).After 2 weeks,the lungs of rats in Group B were harvested for histological observation,preparation of de-cellularized lung scaffolds,and proteomic analysis for small-molecular-weight proteins,and similar procedures were performed on Group C and A after 4 *** differentially expressed small-molecular-weight proteins(DESMPs)between different groups and the subcellular locations were ***::Of the 1626 small-molecular-weight proteins identified,1047 were *** were 97 up-regulated and 45 downregulated proteins in B vs.A,274 up-regulated and 31 down-regulated proteins in C vs.A,and 237 up-regulated and 28 downregulated proteins identified in C *** the up-regulated and down-regulated proteins in the three comparisons were mainly distributed in single-organism processes and cellular processes within biological process,cell and organelle within cellular component,and binding within molecular ***,more up-regulated than down-regulated proteins were identified in most sub-cellular *** interactions of DESMPs identified in extracellular location in all comparisons showed that serum albumin(Alb

关键词： Small molecular protein Extracellular matrix Pulmonary fibrosis Paraquat Biomarkers Rats

Effects of aggregate size distribution and carbon nanotubes on the mechanical properties of cemented gangue backfill samples under true triaxial compression

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 311-324页

作者： Qian Yin Fan Wen Zhigang tao hai Pu Tianci Deng Yaoyao Meng Qingbin Meng Hongwen Jing Bo Meng Jiangyu Wu State Key Laboratory of Intelligent Construction and Healthy Operation and Maintenance of Deep Underground Engineering China University of Mining and TechnologyXuzhou 221116China State Key Laboratory of Mining Response and Disaster Prevention and Control in Deep Coal Mines Anhui University of Science and TechnologyHuainan 232001China State Key Laboratory for Geomechanics&Deep Underground Engineering China University of Mining and Technology(Beijing)Beijing 100083China Key Laboratory of xinjiang Coal Resources Green Mining Ministry of EducationXinjiang Institute of EngineeringUrumqi 830023China

The mechanical behavior of cemented gangue backfill materials(CGBMs)is closely related to particle size distribution(PSD)of aggregates and properties of cementitious ***,the true triaxial compression tests,CT scanning,SEM,and EDS tests were conducted on cemented gangue backfill samples(CGBSs)with various carbon nanotube concentrations(P_(CNT))that satisfied fractal theory for the PSD of *** mechanical properties,energy dissipations,and failure mechanisms of the CGBSs under true triaxial compression were systematically *** results indicate that appropriate carbon nanotubes(CNTs)effectively enhance the mechanical properties and energy dissipations of CGBSs through micropore filling and microcrack bridging,and the optimal effect appears at P_(CNT)of 0.08wt%.Taking PSD fractal dimension(D)of 2.500 as an example,compared to that of CGBS without CNT,the peak strength(σ_(p)),axial peak strain(ε_(1,p)),elastic strain energy(Ue),and dissipated energy(U_(d))increased by 12.76%,29.60%,19.05%,and90.39%,***,excessive CNTs can reduce the mechanical properties of CGBSs due to CNT agglomeration,manifesting a decrease inρ_(p),ε_(1,p),and the volumetric strain increment(Δε_(v))when P_(CNT)increases from 0.08wt%to 0.12wt%.Moreover,the addition of CNTs improved the integrity of CGBS after macroscopic failure,and crack extension in CGBSs appeared in two modes:detour and pass through the ***σ_(p)and U_(d)firstly increase and then decrease with increasing D,and porosity shows the opposite ***ε_(1,p)andΔε_(v)are negatively correlated with D,and CGBS with D=2.150 has the maximum deformation parameters(ε_(1,p)=0.05079,Δε_(v)=0.01990)due to the frictional slip effect caused by coarse *** increasing D,the failure modes of CGBSs are sequentially manifested as oblique shear failure,"Y-shaped"shear failure,and conjugate shear failure.

关键词： cemented gangue backfill materials particle size distribution true triaxial compression test carbon nanotubes mechanical properties failure modes

Mechanistic insight into the synergy between nickel single atoms and nanoparticles on N-doped carbon for electroreduction of CO2

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Journal of Energy Chemistry 2025年第1期100卷 327-336页

作者： Mingdong Sun Wenwen Guan Cailing Chen Chao Wu xiaoling Liu Biao Meng tao Chen Yu Han Jun Wang Shibo xi Yu Zhou State Key Laboratory of Materials-Oriented Chemical Engineering College of Chemical Engineering Nanjing Tech University Advanced Membranes and Porous Materials Center Physical Sciences and Engineering Division King Abdullah University of Science and Technology Institute of Sustainability for Chemicals Energy and Environment A*STAR (Agency for Science Technology and Research) College of Materials Science and Engineering Sichuan University

The synergy of single atoms （SAs） and nanoparticles （NPs） has demonstrated great potential in promoting the electrocatalytic carbon dioxide reduction reaction （CO2RR）;however,the rationalization of the SAs/NPs proportion remains one challenge for the catalyst ***,a Ni2+-loaded porous poly（ionic liquids）(PIL) precursor synthesized through the free radical self-polymerization of the ionic liquid monomer,1-allyl-3-vinylimidazolium chloride,was pyrolyzed to prepare the Ni,N co-doped carbon materials,in which the proportion of Ni SAs and NPs could be facilely modulated by controlling the annealing *** catalyst Ni-NC-1000 with a moderate proportion of Ni SAs and NPs exhibited high efficiency in the electrocatalytic conversion of CO2into *** Ni K-edge X-ray absorption near-edge structure （XANES） spectra and theoretical calculations were conducted to gain insight into the synergy of Ni SAs and *** charge transfer from Ni NPs to the surrounding carbon layer and then to the Ni SAs resulted in the electron-enriched Ni SAs active *** the electroreduction of CO2,the coexistence of Ni SAs and NPs strengthened the CO2activation and the affinity towards the key intermediate of*COOH,lowering the free energy for the potential-determining*CO2→*COOH step,and therefore promoted the catalysis efficiency.

关键词： Activation energy

Potential-based sensitivity estimation for Markov decision processes with general state space

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Potential-based sensitivity estimation for Markov decision p...

弹塑性边界下水下爆炸气泡动态特性的数值仿真研究

第二十一届中国控制会议

作者： FANG hai-tao , CAO xi-ren ( Institute of Systems Science, Academy of Mathematics and Systems Science Chinese Academy of Sciences, Beijing, 100080, China Department of EEE, Hong Kong University of Science and TechnologyClear Water Bay, Kowloon, Hongkong)

＜正＞We propose a unified formulation for the performance sensitivity analysis of Markov chain and MDPs in general state space with both infinite horizon average-cost and discounted performance criteria. And the sens... 详细信息

关键词： Markov decision processes Poisson equations potentials sensitivity analysis W-factors

来源： cnki会议评论

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系统仿真学报 2009年第14期21卷 4532-4535,4539页

作者：李海涛朱锡张振华牟金磊海军工程大学船舶与动力学院武汉430033

水下爆炸气泡与弹塑性结构边界的相互作用问题研究具有重要意义。运用有限元软件***建立三维气泡模型,对水平弹塑性薄板边界条件下的水下爆炸气泡运动特性进行了研究,并分析了流固耦合作用。引入无量纲参数n=爆距/最大气泡半径,分析了不... 详细信息

水下爆炸气泡与弹塑性结构边界的相互作用问题研究具有重要意义。运用有限元软件***建立三维气泡模型,对水平弹塑性薄板边界条件下的水下爆炸气泡运动特性进行了研究,并分析了流固耦合作用。引入无量纲参数n=爆距/最大气泡半径,分析了不同n值条件下的气泡运动参数,将其与无边界影响的气泡参数进行比较,发现n≥2时,可忽略弹性边界影响,气泡作理想球体模型处理;对气泡收缩引起流体出现锥形空化及射流等现象进行了分析,发现该种射流是造成弹性板产生冲击变形、破坏的能量之一,并通过爆炸试验得到证实。

关键词：气泡水下爆炸数值仿真动态响应流固耦合

受精方式、女方年龄、生育史和取卵数对体外异常受精的影响

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生殖与避孕 2011年第11期31卷 746-750页

作者：习海涛陈华葛红山单丹吕杰强温州医学院附属第二医院生殖中心温州325000

目的:探讨人卵子体外受精后单原核(PN)和多原核胚胎形成的影响因素,为提高正常受精率探寻可行的方法。方法:回顾性分析1 004个IVF周期和250个ICSI周期,共计15 364个卵细胞资料,研究胚胎原核形成与体外受精方式、女方年龄、生育史、获卵... 详细信息

目的:探讨人卵子体外受精后单原核(PN)和多原核胚胎形成的影响因素,为提高正常受精率探寻可行的方法。方法:回顾性分析1 004个IVF周期和250个ICSI周期,共计15 364个卵细胞资料,研究胚胎原核形成与体外受精方式、女方年龄、生育史、获卵数的关系。结果:①ICSI周期的1PN率明显高于IVF周期,而3PN和4PN形成率则明显低于IVF(P35岁时,1PN、3PN和4PN形成率均显著高于年龄≤35岁者(P20个卵组(P20枚时ICSI组2PN(正常受精率)生成率显著降低。结论:人体外受精正常与否受到受精方式、女方年龄、生育史和取卵数目等因素的影响,因此,在进行辅助生殖技术(ART)时应综合考虑这些因素,有助于提高正常受精率,降低异常受精率。

关键词：体外受精胚胎单原核(1PN) 3原核(3PN)

2024年美国绿色化学挑战奖项目创新研究进展

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现代化工 2025年第1期45卷 13-16页

作者：程海涛衡水学院河北衡水053000

对美国环保署(EPA)公布的2024年度美国绿色化学挑战奖(GCCA)获奖成果的创新与价值进行了分析与阐述。Merck&***.公司被授予绿色合成路线奖(Greener Synthetic Pathways Award),其创新价值在于发明了一种新的“连续工艺”生产其PD-1疗法K... 详细信息

对美国环保署(EPA)公布的2024年度美国绿色化学挑战奖(GCCA)获奖成果的创新与价值进行了分析与阐述。Merck&***.公司被授予绿色合成路线奖(Greener Synthetic Pathways Award),其创新价值在于发明了一种新的“连续工艺”生产其PD-1疗法KEYTRUDA■(pembrolizumab)用药物。Pro-Farm Group被授予安全与可降解化学品设计奖,开发了一种具有天然杀虫作用的微生物农药RinoTec^(TM)。Viridis化学公司被授予小企业奖,开发了一种利用可再生原料催化合成乙酸乙酯的工艺流程。特拉华大学Dionisios ***教授被授予学术奖(Academic Award),开发了利用可再生原料生产润滑油主要化学成分的新合成方法。PhoSul■研究团队被授予环境特别奖,开发了PhoSul®增强型磷矿肥料。

关键词： GCCA 减排节能安全与可降解化学品设计 2024年

2014年7月9日麦盖提M_S5.1地震前兆异常分析

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内陆地震 2018年第1期32卷 12-23页

作者：张治广高歌滕海涛邢喜民新疆维吾尔自治区地震局新疆乌鲁木齐830011

利用常用的形态分析法和矢量合成法,研究2014年7月9日麦盖提M_S5.1地震的前兆异常特征,并对其异常可靠性进行分析。结果表明:在地震发生前,距震中300 km范围内的南天山西段与和田地区共8套前兆仪器出现群体异常,异常特征主要表现为数据... 详细信息

利用常用的形态分析法和矢量合成法,研究2014年7月9日麦盖提M_S5.1地震的前兆异常特征,并对其异常可靠性进行分析。结果表明:在地震发生前,距震中300 km范围内的南天山西段与和田地区共8套前兆仪器出现群体异常,异常特征主要表现为数据的速率快速变化、反向变化、年变畸变等。最后,对异常时空分布特征进行分析,对塔里木盆地内部发生5级以上地震对新疆及周边后续强震的指示意义进行了初步讨论。

关键词：地震前兆异常分析时空分析数字化观测资料