Small molecule proteomics quantifies differences between normal and fibrotic pulmonary extracellular matrices

作     者：Xin-Long Wan Zhi-Liang Zhou Peng Wang Xiao-Ming Zhou Meng-Ying Xie Jin Mei Jie Weng Hai-Tao Xi Chan Chen Zhi-Yi Wang Zhi-Bin Wang Wan Xin-Long;Zhou Zhi-Liang;Wang Peng;Zhou Xiao-Ming;Xie Meng-Ying;Mei Jin;Weng Jie;Xi Hai-Tao;Chen Chan;Wang Zhi-Yi;Wang Zhi-Bin

作者机构：Platform for Radiation Protection and Emergency Preparedness of Southern ZhejiangSchool of Public Health and ManagementWenzhou Medical UniversityWenzhouZhejiang 325035China Center for Health AssessmentWenzhou Medical UniversityWenzhouZhejiang 325035China Department of Emergency Medicine and General PracticeThe Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical UniversityWenzhouZhejiang 325027China Department of Geriatric MedicineThe First Affiliated Hospital of Wenzhou Medical UniversityWenzhouZhejiang 325000China lnstitute of Bioscaffold Transplantation and ImmunologySchool of Basic Medical SciencesWenzhou Medical UniversityWenzhouZhejiang 325035China. 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2020年第133卷第10期

页      面：1192-1202页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：This study was supported by grants from the Zhejiang Provincial Natural Science Foundation of China(No.LQ17H010004 and No.LQ16H040002) the National Natural Science Foundation of China(No.81772054 and No.81701379) the Zhejiang Medicines Health Science and Technology Program(No.2016KYB189) the Wenzhou Science and Technology Bureau Program(No.Y20170179) 

主　　题：Small molecular protein Extracellular matrix Pulmonary fibrosis Paraquat Biomarkers Rats 

摘      要：Background:Pulmonary fibrosis is a respiratory disease caused by the proliferation of fibroblasts and accumulation of the extracellular matrix(ECM).It is known that the lung ECM is mainly composed of a three-dimensional fiber mesh filled with various high-molecular-weight ***,the small-molecular-weight proteins in the lung ECM and their differences between normal and fibrotic lung ECM are largely ***::Healthy adult male Sprague-Dawley rats(Rattus norvegicus)weighing about 150 to 200 g were randomly divided into three groups using random number table:A,B,and C and each group contained five *** rats in Group A were administered a single intragastric(i.g.)dose of 500μL of saline as control,and those in Groups B and C were administered a single *** of paraquat(PQ)dissolved in 500μL of saline(20 mg/kg).After 2 weeks,the lungs of rats in Group B were harvested for histological observation,preparation of de-cellularized lung scaffolds,and proteomic analysis for small-molecular-weight proteins,and similar procedures were performed on Group C and A after 4 *** differentially expressed small-molecular-weight proteins(DESMPs)between different groups and the subcellular locations were ***::Of the 1626 small-molecular-weight proteins identified,1047 were *** were 97 up-regulated and 45 downregulated proteins in B vs.A,274 up-regulated and 31 down-regulated proteins in C vs.A,and 237 up-regulated and 28 downregulated proteins identified in C *** the up-regulated and down-regulated proteins in the three comparisons were mainly distributed in single-organism processes and cellular processes within biological process,cell and organelle within cellular component,and binding within molecular ***,more up-regulated than down-regulated proteins were identified in most sub-cellular *** interactions of DESMPs identified in extracellular location in all comparisons showed that serum albumin(Alb