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Establishment of an indirect immunofluorescence assay for the detection of African swine fever virus antibodies

Journal of Integrative Agriculture 2024年第1期23卷 228-238页

作者： Wan Wang Zhenjiang zhang Weldu Tesfagaber Jiwen zhang Fang Li Encheng Sun Lijie Tang Zhigao Bu Yuanmao Zhu Dongming Zhao State Key Laboratory for Animal Disease Control and Prevention National African Swine Fever Para-reference LaboratoryNational High Containment Facilities for Animal Diseases Control and PreventionHarbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069China College of Veterinary Medicine Northeast Agricultural UniversityHarbin 150069China

African swine fever(ASF)continues to cause enormous economic loss to the global pig *** there is no safe and effective vaccine,accurate and timely diagnosis of ASF is essential to implement control *** immunofluorescence assay(IFA)is a gold standard serological method recommended by the World Organization for Animal Health(WOAH).In this study,we used primary fetal kidney cells to establish a wild boar cell line(BK2258)that supported the efficient replication of ASF virus(ASFV)SD/DY-I/21 and showed visible cytopathic effect(CPE).Moreover,using BK2258,we established a sensitive and specific IFA for ASFV antibody *** standardize and evaluate the performance of this assay,we used serum samples from pigs infected with the low virulent genotype I SD/DY-I/21 and genotype II HLJ/HRB1/20,and immunized with the vaccine candidate HLJ/18-7GD,field samples,and negative serum *** IFA reacted with the ASFV-positive sera and displayed bright fluorescence *** was no non-specific green fluorescence due to cellular senescence or other cell damage-causing *** to a commercial indirect enzyme-linked immunosorbent assay(iELISA),ASFV antibodies were detected 1–4 days earlier using our *** detection limits of the IFA and iELISA for the same ASFV-antibody positive serum samples were 1:25,600 and 1:6,400,respectively,indicating that the IFA is more sensitive than *** newly established IFA was highly specific and did not cross-react with sera positive for six other important porcine pathogens(i.e.,Classical swine fever virus(CSFV),Porcine reproductive and respiratory syndrome virus(PRRSV),Porcme circovirus type 2(PCV2),Pseudorabies virus(PRV),Foot-and-Mouth disease virus type O(FMDV/O),and Porcine epidemic diarrhea virus(PEDV)).This study thus provides a sensitive,specific,and reliable detection method that is suitable for the serological diagnosis of ASF.

关键词： African swine fever antibody IFA serological method

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研究生教育强国:概念内涵、核心要素和建设方略

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学位与研究生教育 2024年第7期 50-57页

作者：王战军张微北京理工大学研究生教育研究中心北京100081 北京理工大学教育学院北京100081

研究生教育是建设人才强国、科技强国和文化强国的重要结合点,是培养拔尖创新人才、支撑国家战略发展的基石。研究生教育强国是具有先进的研究生教育理念、完整的研究生教育体系,支撑国家战略发展,为世界科技变革和人类文明进步作出持... 详细信息

研究生教育是建设人才强国、科技强国和文化强国的重要结合点,是培养拔尖创新人才、支撑国家战略发展的基石。研究生教育强国是具有先进的研究生教育理念、完整的研究生教育体系,支撑国家战略发展,为世界科技变革和人类文明进步作出持续重要贡献的国家。研究生教育强国的核心要素是较大的在学规模、合理的培养结构、优良的培养条件、卓越的培养质量、世界级的若干研究生教育“高地”。研究生教育强国建设要坚持立德树人一条工作主线,抓好博士生、留学生两个着力点,突出数智化、多元化、全球化三个重点,聚焦时代定位、体制机制、资源配置、全球视野四个维度。

关键词：研究生教育强国概念要素策略

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北美iSchool院校危机信息学教育研究

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图书馆杂志 2024年第1期43卷 118-128页

作者：张衍韦昱杰上海大学文化遗产与信息管理学院

解决日益频发和日趋复杂的公共危机亟需危机信息管理专业人才。北美iSchool院校在危机信息管理人才的培养上具有丰富的经验和成熟的体系,可为我国开展危机信息学教育提供参考借鉴。文章运用文献分析法和网络调查法,归纳北美iSchool院校... 详细信息

解决日益频发和日趋复杂的公共危机亟需危机信息管理专业人才。北美iSchool院校在危机信息管理人才的培养上具有丰富的经验和成熟的体系,可为我国开展危机信息学教育提供参考借鉴。文章运用文献分析法和网络调查法,归纳北美iSchool院校危机信息学教育模式,从培养目标、课程内容、教学体系、师资力量和学生就业等方面分析其教育体系。针对我国危机信息管理人才需求巨大但教育相对滞后的状态,提出把握学科变革机遇,开展危机信息管理教育;突破学科壁垒,推动多元主体协同办学;回应多维需求,丰富教育体系和教学形式;注重理论与实践结合,建立全面综合的教学、考核机制等建议。

关键词： iSchool 危机信息学危机信息管理教育北美

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Study of leakage current degradation based on stacking faults expansion in irradiated SiC junction barrier Schottky diodes

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Chinese Physics B 2024年第10期33卷 458-464页

作者： Maojiu Luo Yourun zhang Yucheng Wang Hang Chen Rong Zhou Zhi Wang Chao Lu Bo zhang State Key Laboratory of Electronic Thin Films and Integrated Devices School of Integrated Circuit Science and EngineeringUniversity of Electronic Science and Technology of ChinaChengdu 611731China China Zhenhua Group Yongguang Electronics Co. Ltd.Guiyang 550018China

A comprehensive investigation was conducted to explore the degradation mechanism of leakage current in SiC junction barrier Schottky(JBS)diodes under heavy ion *** propose and verify that the generation of stacking faults(SFs)induced by the recombination of massive electron-hole pairs during irradiation is the cause of reverse leakage current degradation based on experiments *** irradiation experiment was carried out based on Ta ions with high linear energy transfer(LET)of 90.5 MeV/(mg/cm^(2)).It is observed that the leakage current of the diode undergoes the permanent increase during irradiation when biased at 20%of the rated reverse ***-PL spectroscopy and PL micro-imaging were utilized to detect the presence of SFs in the irradiated SiC JBS *** combined the degraded performance of irradiated samples with SFs introduced by heavy ion ***,three-dimensional(3D)TCAD simulation was employed to evaluate the excessive electron-hole pairs(EHPs)concentration excited by heavy ion *** was observed that the excessive hole concentration under irradiation exceeded significantly the threshold hole concentration necessary for the expansion of SFs in the *** proposed mechanism suggests that the process and material characteristics of the silicon carbide should be considered in order to reinforcing against the single event effect of SiC power devices.

关键词： 4H-SiC JBS diode heavy ion irradiation single event effect single event leakage current degradation

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大宝山含钨褐铁矿中钨的赋存状态与嵌布特征

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Transactions of Nonferrous Metals Society of China 2024年第4期34卷 1263-1274页

作者：唐鸿鹄刘丙建王翠张雄星韩海生王丽曹杨孙伟中南大学资源加工与生物工程学院长沙410083 中南大学湖南省关键金属矿产资源高效清洁利用国际联合研究中心长沙410083

通过多种分析方法,探究广东大宝山含钨褐铁矿的物相组成、元素分布等工艺矿物学特征,以揭示矿石中关键金属钨的赋存状态与嵌布特征。X射线荧光分析(XRF)、粉末X射线衍射(XRD)以及扫描电子显微镜和能谱(SEM-EDS)结果表明:矿石中主要矿物... 详细信息

通过多种分析方法,探究广东大宝山含钨褐铁矿的物相组成、元素分布等工艺矿物学特征,以揭示矿石中关键金属钨的赋存状态与嵌布特征。X射线荧光分析(XRF)、粉末X射线衍射(XRD)以及扫描电子显微镜和能谱(SEM-EDS)结果表明:矿石中主要矿物为含钨褐铁矿和石英,关键金属钨品位为1.35%。微区X射线衍射(Micro-XRD)和矿物解离度分析仪(MLA)结果表明:钨主要赋存于高铁钨华((W,Fe)(O,OH)_(3))中,而高铁钨华则以剥离和带状形式紧密分布在褐铁矿中。同时,还定量分析了有价元素在各主要矿物中的赋存和分布情况,并讨论了含钨褐铁矿风化演变和形成机制。最终提出了一种选冶联合分选回收流程,为高效回收含钨褐铁矿中关键金属钨提供理论基础。

关键词：含钨褐铁矿赋存状态微区X射线衍射

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Al对Mg-Zn-Y合金组织和拉伸性能的影响

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材料热处理学报 2024年第5期45卷 81-86页

作者：李龙张磊杨伟南昌航空大学航空制造工程学院江西南昌330063

研究了Al对铸态Mg_(95)Zn_(4)Y_(1)合金组织和拉伸性能的影响。结果表明:随着Al含量的增加,合金晶粒呈先细化后粗化的趋势。Mg_(94.7)Zn_(4)Y_(1)Al_(0.3)合金中初生α-Mg相的晶粒最细,其平均二次枝晶臂间距λ为31.9μm,相比Mg_(95)Zn_(... 详细信息

研究了Al对铸态Mg_(95)Zn_(4)Y_(1)合金组织和拉伸性能的影响。结果表明:随着Al含量的增加,合金晶粒呈先细化后粗化的趋势。Mg_(94.7)Zn_(4)Y_(1)Al_(0.3)合金中初生α-Mg相的晶粒最细,其平均二次枝晶臂间距λ为31.9μm,相比Mg_(95)Zn_(4)Y_(1)合金降低了28.6%。添加Al有利于合金中I-phase的析出。随着Al含量的增加,合金的拉伸性能呈先升高后降低的趋势。Mg_(94.7)Zn_(4)Y_(1)Al_(0.3)合金表现出最优的室温拉伸性能,其极限拉伸强度和伸长率分别为211 MPa和9.7%,相比Mg_(95)Zn_(4)Y_(1)合金分别提高了28.7%和27.6%。

关键词： Mg_(95)Zn_(4)Y_(1)合金 Al 组织拉伸性能

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Mechanical Behavior Based on Aggregates Microstructure of Ultra-high Performance Concrete

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Journal of Wuhan University of Technology(Materials Science) 2024年第3期39卷 673-681页

作者：丁庆军 ZHOU Changsheng 张高展 GUO Hong LI Yang zhang Yongyuan GUO Kaizheng School of Materials Science and Engineering Wuhan University of TechnologyWuhan 430070China Advanced Building Materials Key Laboratory of Anhui Province School of Material Science and Chemical EngineeringAnhui Jianzhu UniversityHefei 230601China China Railway 17th Bureau Group Co. Ltd.Taiyuan 030006China

We developed ultra-high performance concrete(UHPC)incorporating mullite sand and brown corundum sand(BCS),and the quartz sand UHPC was utilized to prepare for *** properties of compressive strength,elastic modulus,ultrasonic pulse velocity,flexural strength,and toughness were *** electron microscopy and nanoindentation were also conducted to reveal the underlying mechanisms affecting macroscopic *** to the superior interface bonding properties between mullite sand and matrix,the compressive strength and flexural toughness of UHPC have been significantly *** sand and BCS aggregates have higher stiffness than quartz sand,contributing to the excellent elastic modulus exhibited by *** stiffness and volume of aggregates have a more significant impact on the elastic modulus of UHPC than interface performance,and the latter contributes more to the strength of *** study will provide a reference for developing UHPC with superior elastic modulus for structural engineering.

关键词： ultra-high performance concrete mechanical properties fine aggregates microstructure nanoindentation

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Data collection of wireless sensor network based on trajectory optimization of laser-charged UAV

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High-Confidence Computing 2024年第2期4卷 116-128页

作者： Chuanwen Luo Jian zhang Jin Qian Yi Hong Zhibo Chen Yunan Hou Xiujuan zhang Yuqing Zhu School of Information Science and Technology Beijing Forestry UniversityBeijing 100083China Engineering Research Center for Forestry-Oriented Intelligent Information Processing of National Forestry and Grassland Administration Beijing 100083China College of Information Engineering Taizhou UniversityTaizhou 225300China School of Computer Science Qufu Normal UniversityRizhao 276826China Research Center of Applied Mathematics and Machine Intelligence Zhejiang LabHangzhou 311121China Department of Computer Science California State UniversityCA 90032USA

Unmanned Aerial Vehicle(UAV)can be used as wireless aerial mobile base station for collecting data from sensors in UAV-based Wireless Sensor Networks(WSNs),which is crucial for providing seamless services and improving the performance in the next generation wireless ***,since the UAV are powered by batteries with limited energy capacity,the UAV cannot complete data collection tasks of all sensors without energy replenishment when a large number of sensors are deployed over large monitoring *** overcome this problem,we study the Real-time Data Collection with Lasercharging UAV(RDCL)problem,where the UAV is utilized to collect data from a specified WSN and is recharged using Laser Beam Directors(LBDs).This problem aims to collect all sensory data from the WSN and transport it to the base station by optimizing the flight trajectory of UAV such that realtime data performance is ensured It has been proven that the RDCL problem is *** address this,we initially focus on studying two sub-problems,the Trajectory Optimization of UAV for Data Collection(TODC)problem and the Charging Trajectory Optimization of UAV(CTO)problem,whose objectives are to find the optimal flight plans of UAV in the data collection areas and charging areas,*** we propose an approximation algorithm to solve each of them with the constant ***,we present an approximation algorithm that utilizes the solutions obtained from TODC and CTO problems to address the RDCL ***,the proposed algorithm is verified by extensive simulations.

关键词： Wireless sensor networks Laser-charged UAV Trajectory optimization Data collection

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Effects of Health Education with Problem-Based Learning Approaches on the Knowledge, Attitude, Practice and Coping Skills of Women with High-Risk Pregnancies in Plateau Areas

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Open Journal of Nursing 2024年第5期14卷 192-199页

作者： Ying Wu Suolang Sezhen Renqing Yuzhen Hong wei Zhijuan Zhan Baima Hongying Yuhong zhang Lihong Liu Department of Nursing Linzhi People&#8217s Hospital Linzhi China Department of Obstetrics and Gynecology Linzhi People&#8217s Hospital Linzhi China

Objective: Given the unique cultural background, way of life, and physical environment of the Tibetan Plateau, this study aims to investigate the effects of health education using problem-based learning (PBL) approaches on the knowledge, attitude, practice, and coping skills of women with high-risk pregnancies in this region. Methods: 76 high-risk pregnancy cases were enrolled at Tibet’s Linzhi People’s Hospital between September 2023 and April 2024. 30 patients admitted between September 2023 and December 2023 were selected as the control group and were performed with regular patient education. 46 patients admitted between January 2024 and April 2024 were selected as the observation group and were performed regular patient education with problem-based learning approaches. Two groups’ performance on their health knowledge, attitude, practice and coping skills before and after interventions were evaluated, and patient satisfaction were measured at the end of the study. Results: There was no statistical significance (P P P Conclusions: Health education with problem-based learning approaches is worth promoting as it can help high-risk pregnant women in plateau areas develop better health knowledge, attitude and practice and healthier coping skills. Also, it can improve patient sanctification.

关键词： Plateau Areas Patients with High-Risk Pregnancies Problem-Based Learning Health Education Health Knowledge Attitude and Practice Coping Skills

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Panoramic variation analysis of a family with neurodevelopmental disorders caused by biallelic loss-of-function variants in TMEM141,DDHD2,and LHFPL5

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Frontiers of Medicine 2024年第1期18卷 81-97页

作者： Liwei Sun Xueting Yang Amjad Khan Xue Yu Han zhang Shirui Han Xiaerbati Habulieti Yang Sun Rongrong Wang Xue zhang McKusick-zhang Center for Genetic Medicine State Key Laboratory for Complex Severe and Rare DiseasesInstitute of Basic Medical Sciences Chinese Academy of Medical SciencesSchool of Basic Medicine Peking Union Medical CollegeBeijing100005China Chongqing Key Laboratory of Human Embryo Engineering Center for Reproductive MedicineNational Key Clinical Speciality Construction Project(Obstetrics and Gynecology)Chongqing Health Center for Women and ChildrenChongqing400013China Chongqing Clinical Research Center for Reproductive Medicine Women and Children’s Hospital of Chongqing Medical UniversityChongqing400013China Faculty of Biological Sciences Department of ZoologyUniversity of Lakki MarwatKhyber Pakhtunkhwa28420Pakistan Institute for Medical Genetics and Applied Genomics University of TübingenTübingen72076Germany Alexander von Humboldt fellowship Foundation Berlin10117Germany Department of Pediatrics The First Affiliated Hospital of Guangxi Medical UniversityNanning530000China Department of Laboratory Medicine State Key Laboratory for Complex Severe and Rare DiseasesPeking Union Medical College HospitalChinese Academy of Medical Science and Peking Union Medical CollegeBeijing100730China

Highly clinical and genetic heterogeneity of neurodevelopmental disorders presents a major challenge in clinical genetics and *** variation analysis is imperative to analyze the disease phenotypes resulting from multilocus genomic ***,a Pakistani family with parental consanguinity was presented,characterized with severe intellectual disability(ID),spastic paraplegia,and *** mapping,integrated single nucleotide polymorphism(SNP)array,whole-exome sequencing,and whole-genome sequencing were performed,and homozygous variants in TMEM141(c.270G>A,***90^(*)),DDHD2(c.411+767_c.1249-327del),and LHFPL5(c.250delC,***84^(*))were identified.A Tmem141^(***90^(*)/***90^(*))mouse model was *** studies showed impairments in learning ability and motor *** slice electrophysiology and Golgi staining demonstrated deficient synaptic plasticity in hippocampal neurons and abnormal dendritic branching in cerebellar Purkinje *** electron microscopy showed abnormal mitochondrial ***,studies on a human in vitro neuronal model(SH-SY5Y cells)with stable shRNA-mediated knockdown of TMEM141 showed deleterious effect on bioenergetic function,possibly explaining the pathogenesis of replicated phenotypes in the cross-species mouse ***,panoramic variation analysis revealed that multilocus genomic variations of TMEM141,DDHD2,and LHFPL5 together caused variable phenotypes in ***,the biallelic loss-of-function variants of TMEM141 were responsible for syndromic ID.

关键词： neurodevelopmental disorder autosomal recessive intellectual disability consanguinity spastic paraplegia hearing loss TMEM141

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