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Effect of 5-Aza-2’-deoxycytidine on immune-associated proteins in exosomes from hepatoma

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World Journal of gastroenterology 2010年第19期16卷 2371-2377页

作者： gao-wa Sanren Department of Histology and Embryology Inner Mongolia Medical College Hohhot 010010 Inner Mon-golia Autonomous Region China

AIM: To study the effect of 5-Aza-2’-deoxycytidine (5-Aza-CdR) on heat shock protein 70 (HSP70), human leucocyte antigen-Ⅰ (HLA-Ⅰ) and NY-ESO-1 proteins in exosomes produced by hepatoma cells, Hepg2 and Hep3B. METHODS: Exosomes derived from Hepg2 and Hep3B cells treated with or without 5-aza-CdR were isolated and purified by ultrafiltration centrifugation and sucrose gradient ultracentrifugation. The number of exosomes was counted under electron microscope. Concentration of proteins in exosomes was measured by bicinchoninic acid protein assay. Expression of HSP70, HLA-Ⅰ and NY-ESO-1 proteins in exosomes was detected by Western blotting and immunoelectron microscopy. mRNA expression of p53 gene was detected by reverse transcription polymerase chain ***: The mRNA expression of p53 gene was increased in both hepatoma cell lines after treatment with 5-Aza-CdR. The number of exosomes and the concentration of total proteins in exosomes were increased signifi cantly after treatment with 5-aza-CdR (P g showed that the HSP70, HLA-Ⅰ and NY-ESO-1 proteins were increased in exosomes produced by both hepatoma cell lines. CONCLUSION: 5-aza-CdR, an inhibitor of DNA methyltransferase, can increase exosomes produced by hepatoma cells and immune-associated protein component of exosomes, which may be mediated by p53 gene upregulation and 5-Aza-CdR demethylation.

关键词： 5-Aza-2’-deoxycytidine Exosome Immu-nomolecule Hepatoma cell

Alleviation of arsenic toxicity by phosphate is associated with its regulation of detoxification,defense,and transport gene expression in barley

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Journal of Integrative Agriculture 2019年第2期18卷 381-394页

作者： gerald Zvobgo Jonas Lwalaba wa Lwalaba Tichaona Sagonda James Mutemachani Mapodzeke Noor Muhammad Imran haider Shamsi ZhaNg guo-ping Department of Agronomy College of Agriculture and BiotechnologyZhejiang University/Zhejiang Key Laboratory of Crop Germplasm ResourceHangzhou 310058P.R.China

Arsenic(As) contamination in soils has posed a severe threat to safe crop production. The previous studies showed the antagonism between phosphorus(P) and As in plant growth and As uptake, while the mechanisms of alleviating As toxicity by P is not completely clear. Due to the limiting P condition, it is imperative to understand how low P addition can be used to suppress arsenate As(V) uptake and the subsequent mechanisms involved. Thus in this study we investigated the effect of P addition on As uptake, anti-oxidative enzyme activity, and anti-oxidant content, and the relative expression of transport, defense, and detoxification genes using two barley genotypes differing in As toxicity tolerance. P addition significantly reduced As concentration in plant tissues, and caused the great changes in activities of catalase and superoxide dismutase, glutathione content, and the relative expression of examined genes when the plants of the two barley genotypes were exposed to 100 μmol L^(–1) As, with ZDB160(As-tolerant) being much more affected than ZDB475(As-sensitive). The current results show that P addition can alleviate As toxicity by regulating the expression of As transport, defense, and detoxification genes to a greater extent in As tolerance of barley, suggesting the possibility of controlling As uptake and toxicity by applying low amount of P fertilizers in the As-contaminated soils.

关键词： arsenate phosphate transporter tolerance toxicity

Identities Involving Powers and Inverse of Binomial Coefficients

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Journal of Mathematical Research and Exposition 2011年第6期31卷 1021-1029页

作者： Wu Yun g ao wa Department of Mathematics College of Sciences and Technology Inner Mongolia University Inner Mongolia 010021 P. R. China

In this paper,we give several identities of finite sums and some infinite series involving powers and inverse of binomial coefficients,which extends the results of ***.

关键词： inverse of binomial coefficient identities stirling numbers

The use of Phanerochaete chrysosporium,Trichoderma harzianum and Trichoderma viride for biogas production

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Studies in Fungi 2020年第1期5卷 368-380页

作者： Mahmoud YA-g Awadalla OA Estafanous AN Etawy wa Tanta University Faculty of ScienceBotany and Microbiology DepartmentMycology Research Lab.Tanta 31527Egypt Agriculture Microbiology Department SoilWater and Environment Research InstituteAgriculture Research CenterCairoEgypt

The expanding cost of ordinary fuel in urban zones requires the investigation of other vitality *** exploitation of agricultural wastes for biogas production could protect our environment and can also solve emerging energy crises *** chrysosporium,Trichoderma virdie and Trichoderma harzianum were used to ferment rice straw and cattle dung for production of compost and *** yield was determined using displacement technique,using acidified water(1 N H_(2)SO_(4))to prevent solubilization of carbon dioxide contained in the *** results showed that the highest rate of biogas and methane were recorded with treated rice straw mixed with cattle dung where the amount of production from the total gas was 51.84 of biogas and 37.86 of methane per Liter(L)/Kilogram(Kg)of fermented ***,the results showed that the consumed biogas and methane related to volatile solids gave the highest amount with untreated rice straw(86.32 and 56.72 L/Kg respectively)followed by treated rice straw and untreated and treated mixtures of rice straw and cattle dung *** results also showed that the highest bacterial count was obtained from fungi treated rice straw mixed with cattle dung(125×10^(3)cells/g)followed by treated rice straw(32×10^(3)cells/g).This study confirms that ***,*** and *** significantly speed up the process of aerobic and anaerobic fermentation of rice straw in respect to biogas and methane production.

关键词： Aerobic fermentation Anaerobic fermentation Methane Rice straw

A building-based data capture and data mining technique for air quality assessment

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Frontiers of Environmental Science & Engineering 2011年第4期5卷 543-551页

作者： Ni SHENg U wa TANg Faculty of Management and Administration Macao University of Science and TechnologyMacaoChina Department of Civil and Environmental Engineering University of MacaoMacaoChina Dirccao dos Servicos de Proteccao Ambiental MacaoChina

Recently,a building-based air quality model system which can predict air quality in front of individual buildings along both sides of a road has been *** the Macao Peninsula as a case study,this paper shows the advantages of building-based model system in data capture and data *** with the traditional grid-based model systems with input/output spatial resolutions of 1-2 km,the building-based approach can extract the street configuration and traffic data building by building and therefore,can capture the complex spatial variation of traffic emission,urban geometry,and air *** non-homogeneous distribution of air pollution in the Macao Peninsula was modeled in a highspatial resolution of 319 receptors·km^(-2).The spatial relationship among air quality,traffic flow,and urban geometry in the historic urban area is *** study shows that the building-based approach may open an innovative methodology in data mining of urban spatial data for environmental *** results are particularly useful to urban planners when they need to consider the influences of urban form on street environment.

关键词： traffic air pollution spatial distribution high resolution geographic information system

Sol-gel Template Synthesis and Photoluminescence Properties of (Pb_(0.5)Sr_(0.5))TiO_(3) Nanotube Arrays

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Chinese Physics Letters 2011年第7期28卷 278-281页

作者： JIANg Yan-ping wa.g Yu ChaN Lai wa Helen TANg Xin-gui ZHOU Yi-Chun Key Laboratory of Low-Dimensional Materials and Application Technology Xiangtan UniversityXiangtan 411105 Department of Applied Physics and Materials Research Centre Hong Kong Polytechnic UniversityHung HomKowloonHong KongChina School of Physics&Optoelectric Engineering and Guangzhou Higher Education Mega CenterGuangdong University of TechnologyGuangzhou 510006

Lead strontium titanate(Pb_(0.5)Sr_(0.5))TiO_(3)(PST)nanotube arrays are prepared by a sol-gel and spin-coating process using an anodic aluminium oxide *** structure and morphology of the as-prepared sample are characterized by x-ray diffraction,scanning electron microscopy and transmission electron *** results show that the as-prepared PST sample possesses a perovskite structure with a relatively uniform diameter of about 200 ***(PL)spectrum of the sample shows emission bands centered at about 2.87 eV at room temperature,which consists of three intense emission sub-bands at 3.02,2.87 and 2.76 eV,*** energy corresponding to the central PL peak is lower than the band-gap energy(3.2 eV)of PST bulk materials,which may originate from the size effect of the PST nanotube *** multi-peaks in the PL spectrum may be attributed to the radiative recombination of trapped electrons and holes in tail and gap states induced by local defect and oxygen *** Raman scattering spectrum of the PST nanotube arrays,which are obviously broadened in comparison with the bulk materials,show that their phonon modes are in agreement with those allowed in the tetragonal phase.

关键词： template perovskite bands

FLOW-PIPE-SEEPAgE COUPLINg ANALYSIS OF SPANNINg INITIATION OF A PARTIALLY-EMBEDDED PIPELINE

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Journal of Hydrodynamics 2010年第4期22卷 478-487页

作者： gAO Fu-ping LUO Cheng-cai Key Laboratory for Hydrodynamics and Ocean Engineering Institute of Mechanics Chinese Academy of Sciences Beijing 100190 China Centre for Offshore Foundation Systems The University of Western Australia Nedlands WA 6907 Australia

The initiation of pipeline spanning involves the coupling between the flow over the pipeline and the seepage-flow in the soil underneath the *** pipeline spanning initiation is experimentally observed and discussed in this *** is qualitatively indicated that the pressure-drop induced soil seepage failure is the predominant cause for pipeline spanning initiation.A flow-pipe-seepage sequential coupling Finite Element Method（FEM） model is proposed to simulate the coupling between the water flow-field and the soil seepage-field.A critical hydraulic gradient is obtained for oblique seepage failure of the sand in the direction tangent to the *** study is performed to investigate the effects of inflow velocity,pipe embedment on the pressure-drop,and the effects of soil internal friction angle and pipe embedment-to-diameter ratio on the critical flow velocity for pipeline spanning *** is indicated that the dimensionless critical flow velocity changes approximately linearly with the soil internal friction angle for the submarine pipeline partially-embedded in a sandy seabed.

关键词： submarine pipeline pipeline spanning seepage failure flow-pipe-seepage coupling

Application of Shear wave Elastography and Contrast-Enhanced Ultrasound in Transrectal Prostate Biopsy

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Current Medical Science 2022年第2期42卷 447-452页

作者： gao-wa ShaREN Jun ZhaNg Department of Ultrasound Affiliated Hospital of Inner Mongolia Medical UniversityHohhot010050China Department of Urology Affiliated Hospital of Inner Mongolia Medical UniversityHohhot010050China

Objective To explore the clinical value of ultrasound shear wave elastography(SWE)and contrast-enhanced ultrasound(CEUS)in transrectal prostate *** A total of 54 patients(average age:67.79±12.01 years)in the experimental group underwent transrectal prostate biopsy under the guidance of SWE,while 46 patients(average age:69.22±11.54 years)in the control group underwent transrectal prostate biopsy guided by *** There were a total of 451 needles,with an average of 8.35±1.67 needles per patient in the experimental group,and a total of 462 needles,with an average of 10.04±1.33 needles per patient in the control *** difference in puncture times between the two groups was statistically significant(Pwas no significant difference in the positive detection rate,sensitivity or specificity between the two groups(P>0.05),but there was a significant difference in the diagnostic accuracy between the two groups(Pgnificantly higher in the experimental group than in benign prostatic hyperplasia(Pg characteristic curve(ROC)analysis showed that the area under the ROC curve(AUC)of E_(mean)was 0.752(S.E.=0.072,95%CI=0.611-0.894,P=0.007),and the best cutoff value was 47.005 *** In summary,both SWE-and CEUS-guided transrectal prostate biopsy can help find the focus and guide the puncture,and improve the positive detection rate.

关键词： prostate puncture ultrasonic shear wave elastography contrast-enhanced ultrasound biopsy analysis

Advances in photothermal conversion of carbon dioxide to solar fuels

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Journal of Energy Chemistry 2023年第8期83卷 62-78,I0004页

作者： wa.gao Yinwen Li Dequan Xiao Ding Ma College of Bioscience and Resources Environment Beijing University of AgricultureBeijing 102206China College of Chemistry and Molecular Engineering and College of Engineering Peking UniversityBeijing 100871China Center for Integrative Materials Discovery Department of Chemistry and Chemical and Biomedical EngineeringUniversity of New HavenWest HavenCT 06516USA

Converting carbon dioxide(CO_(2)) into useful fuels or chemical feedstocks is important for achieving peak carbon emission and carbon ***,photothermal catalysis has been extensively studied and applied due to its advantages over traditional heat-driven *** this review,we focus on photothermal catalysis of thermodynamically uphill reactions that convert CO_(2)into value-added *** first introduce the fundamentals of photothermal catalysis for CO_(2)reduction,including the definition and classification of photothermal catalysis,followed by their photothermal conversion *** structure design of different types of photothermal catalysts is *** superior performance of photothermal catalytic conversion of CO_(2)is illustrated and discussed,including improved CO_(2)activation,tunable selectivity towards different solar fuel products,and resistance to sintering and ***,the perspectives and challenges in this cutting-edge field are presented with the aim of advancing understanding of the underlying mechanisms and inspiring rational design of photothermal catalysts for highly efficient solar-to-fuel conversion.

关键词： Solarenergy Photothermal catalysis CO_(2)reduction Catalyst design Performance improvement