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优质食品酶的催化机制及在高端配料创制方面的应用基础

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中国食品学报 2024年第5期24卷 75-88页

作者：李兆丰陈福生江正强王永华路福平黄和江南大学食品学院江苏无锡214122 华中农业大学食品科学技术学院武汉430070 中国农业大学食品科学与营养工程学院北京100083 华南理工大学食品科学与工程学院广州510641 天津科技大学生物工程学院工业酶国家工程实验室天津300457 南京师范大学食品与制药工程学院南京210023

优质食品酶是食品生物加工的核心,是食品制造产业的“芯片”。我国食品酶领域的应用基础研究薄弱,70%以上的酶制剂市场被国际公司垄断,优质食品酶长期受制于人,难以支撑目前食品行业提质升级的发展需求。在此背景下,本文聚焦新型食品酶... 详细信息

优质食品酶是食品生物加工的核心,是食品制造产业的“芯片”。我国食品酶领域的应用基础研究薄弱,70%以上的酶制剂市场被国际公司垄断,优质食品酶长期受制于人,难以支撑目前食品行业提质升级的发展需求。在此背景下,本文聚焦新型食品酶高效筛选和分泌表达的生物学基础,高端高值食品酶的催化机制,食品酶催化性能改造的结构基础,食品酶创制高端配料的内在规律及作用机制等关键科学问题,综述优质食品酶的催化机制及在高端配料创制方面的应用基础,旨在为食品高端配料的酶法创制提供理论和实践指导。

关键词：新型食品酶催化机制结构基础高端配料

射流式泡沫分离器中气水比对水处理效率的影响

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渔业现代化 2024年第4期51卷 73-81页

作者：姜浩柳瑶宋协法李贤董登攀黄志涛中国海洋大学水产学院山东青岛266003 山东大学海洋学院山东威海264209 挪威水研究所挪威卑尔根5006

循环水养殖具有节能、节水、环保等优点,是国际先进的养殖模式代表之一。泡沫分离器作为循环水养殖系统的重要设备,其结构设计、操作管理对系统的净水效果有重要影响。目前泡沫分离器运行调节管理参数大多依靠经验,缺乏理论支撑。以应... 详细信息

循环水养殖具有节能、节水、环保等优点,是国际先进的养殖模式代表之一。泡沫分离器作为循环水养殖系统的重要设备,其结构设计、操作管理对系统的净水效果有重要影响。目前泡沫分离器运行调节管理参数大多依靠经验,缺乏理论支撑。以应用较广的射流式泡沫分离器为研究对象,采用计算流体力学(CFD)方法,探究射流式泡沫分离器在不同气水比条件下(1∶2、1∶2.5、1∶3)泡沫的产生效率;采用实机试验探查不同气水比对悬浮颗粒物去除效率的影响。CFD结果显示气水比为1∶2.5时,泡沫分类器产生的气泡数量最多;实机试验结果显示,当气水比为1∶2、1∶2.5、1∶3时,泡沫分离器对固体颗粒的去除效率分别为35.23%±0.77%、44.85%±0.81%、44.52%±0.58%,其中气水比1∶2.5、1∶3的泡沫分离器相比于气水比1∶2的泡沫分离器固体颗粒去除率显著性提高。综上,射流式泡沫分离器气水比建议设定为1∶2.5,可获得较优颗粒物去除率及较好的出泡效果。本研究为优化泡沫分离器的运行参数提供了新的思路。

关键词：射流式泡沫分离器气水比悬浮物去除率数值模拟

Dynamic thermal coupling modeling and analysis of wet electro-hydrostatic actuator

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Chinese Journal of Aeronautics 2022年第6期35卷 298-311页

作者： Zongxia JIAO Yanpeng li Tian YU Chaofan jiang ligang huang Yaoxing sHANG school of Automation science and Electrical Engineering Beihang UniversityBeijing 100083China science and technology on Aircraft Control Laboratory Beihang UniversityBeijing 100083China Research institute for Frontier science Beihang University100083 BeijingChina Ningbo institute of technology Beihang UniversityNingbo 315800China

The electro-hydrostatic actuator(EHA)used in more electric aircraft(MEA)has been extensively studied due to its advantages of high reliability and high ***,this high integration results in a small heat dissipation area,leading to high-temperature ***,to reduce the temperature,a wet cooling method of using the pump leakage oil to cool the motor is adopted,which can also increase the difficulty of accurately predicting the system temperature in the early design *** solve this problem,a dynamic coupling thermal model of a wet EHA is proposed in this *** particular,the leakage oil of the pump is used as a coupling item between the electrical system and the hydraulic ***,an improved T-equivalent block model is proposed to address the uneven distribution of axial oil temperature inside the motor,and the control node method is applied to hydraulic system thermal ***,a dynamic coupling thermal model is developed that enables a dynamic evaluation of the wet EHA ***,experimental prototypes of wet motor and wet EHA are developed,while the temperature response of the wet motor at different rotation speeds and different loads and the temperature response of the wet EHA at no-load condition were verified experimentally at room temperature,*** maximum temperature difference between the experimental and theoretical results of the wet motor as well as the experimental and theoretical results of the wet EHA is less than 8℃.These test results indicate that the dynamic coupling thermal model is valid and demonstrate that the thermal coupling modeling method proposed in this paper can provide a basis for the detailed thermal design of EHA.

关键词： Electro-hydrostatic actuator Hydraulic More electric aircraft Thermal mode Wet motor

Manipulating dielectric property of polymer coatings toward highretention-rate lithium metal full batteries under harsh critical conditions

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Nano Research 2023年第7期16卷 9240-9249页

作者： Qi Kang Zechao Zhuang Yong li Yinze Zuo Jian Wang Yijie liu Chaoqun shi Jie Chen Hongfei li Pingkai jiang Xingyi huang Department of Polymer science and Engineering Shanghai Key Laboratory of Electrical Insulation and Thermal AgeingSchool of Chemistry and Chemical EngineeringShanghai Jiao Tong UniversityShanghai 200240China Department of Chemistry Tsinghua UniversityBeijing 100084China institute of applied and Physical Chemistry and Center for Environmental Research and sustainable technology University of BremenBremen 28359Germany institute for sustainable Energy/college of sciences Shanghai UniversityShanghai 200444China Helmholtz institute Ulm(HIU) Ulm D89081Germany school of Electrical Engineering Xi’an Jiaotong UniversityXi’an 710049China

lithium(li)metal batteries(LMBs)can potentially deliver much higher energy density but remain plagued by uncontrollable li plating with dendrite growth,unstable interfaces,and highly abundant excess li(>50 mAh·cm^(-2)).Herein,different from the artificial layer or three-dimensional(3D)matrix host constructions,various dielectric polymers are initially well-comprehensively investigated from experimental characterizations to theoretical simulation to evaluate their functions in modulating li ion *** a proof of concept,a 3D interwoven high dielectric functional polymer(HDFP)nanofiber network with polar C-F dipole moments electrospun on copper(Cu)foil is designed,realizing uniform and controllable li deposition capacity up to 5.0 mAh·cm^(-2),thereby enabling stable li plating/stripping cycling over 1400 h at 1.0 mA·cm^(-2).More importantly,under the highcathode loading(~3.1 mAh·cm^(-2))and only 0.6×excess li(N/P ratio of 1.6),the full cells retain capacity retention of 97.4%after 200 cycles at 3.36 mA·cm^(-2)and achieve high energy density(297.7 Wh·kg^(-1)at cell-level)under lean electrolyte conditions(15μL),much better than ever-reported *** work provides a new direction for designing high dielectric polymer coating toward high-retention-rate practical li full batteries.

关键词： high dielectric functional polymer nanofiber li metal full cell low N/P ratio high-retention capacity

基于质构仪TPA模式构建蛋白肠感官评分预测模型及应用

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中国调味品 2024年第4期49卷 44-49页

作者：朱美淋张贞炜余杰黄潇漪蒋雨含邢沁浍贾利蓉四川大学轻工科学与工程学院成都610065 宜宾四川大学产业技术研究院四川宜宾644000 新希望集团有限公司成都611400

为建立一套客观、数据化评价动植物双蛋白肠感官综合评分的方法,该研究采用质构剖面分析(TPA)测定24组不同配方的样品,结合感官评价方法,通过逐步回归分析建立感官综合评分预测模型,并将其应用于响应面实验以优化双蛋白肠的配方。结果表... 详细信息

为建立一套客观、数据化评价动植物双蛋白肠感官综合评分的方法,该研究采用质构剖面分析(TPA)测定24组不同配方的样品,结合感官评价方法,通过逐步回归分析建立感官综合评分预测模型,并将其应用于响应面实验以优化双蛋白肠的配方。结果表明,动植物双蛋白肠的感官综合评分预测模型为Y=-68.110+0.003×硬度+79.119×弹性-0.001×咀嚼性-18.393×回复性,该模型相关系数R~2为0.902,校正决定系数R_(Adj)~2为0.875;响应面回归模型相关系数R~2为0.962,校正决定系数R_(Adj)~2为0.924,预测系数R_(Pred)~2为0.861,优化后蛋白肠的最佳配方为大豆拉丝蛋白质量分数74%、猪肉质量分数16%、大豆分离蛋白与花生蛋白粉比例7∶3、猪肉肥瘦比例7∶3,该配方下产品的感官评分实际值为8.51,与理论预测值(8.45)误差较小。该研究得到的动植物双蛋白肠感官综合评分预测模型的拟合度较高,实测值与预测值的吻合度较好,模型在响应面实验中具有可行性,为客观、快捷地评价新型肉肠的感官品质提供了新思路。

关键词：动植物双蛋白肠质构剖面分析感官综合评分回归模型响应面实验

CEPC Technical Design Report

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Radiation Detection technology and Methods 2024年第1期8卷 I0003-I0016,1-1091页

作者： Waleed Abdallah Tiago Carlos Adorno de Freitas Konstantin Afanaciev shakeel Ahmad Ijaz Ahmed Xiaocong Ai Abid Aleem Wolfgang Altmannshofer Fabio Alves Weiming An Rui An Daniele Paolo Anderle stefan Antusch Yasuo Arai Andrej Arbuzov Abdesslam Arhrib Mustafa Ashry sha Bai Yu Bai Yang Bai Vipul Bairathi Csaba Balazs Philip Bambade Yong Ban Tripamo Bandyopadhyay shou-shan Bao Desmond P.Barber Ayse Bat Varvara Batozskaya subash Chandra Behera Alexander Belyaev Michele Bertucci Xiao-Jun Bi Yuanjie Bi Tianjian Bian Fabrizio Bianchi Thomas Biekotter Michela Biglietti shalva Bilanishvili Deng Binglin Denis Bodrov Anton Bogomyagkov serge Bondarenko stewart Boogert Maarten Boonekamp Marcello Borri Angelo Bosotti Vincent Boudry Mohammed Boukidi Igor Boyko Ivanka Bozovic Giuseppe Bozzi Jean-Claude Brient Anastasiia Budzinskaya Masroor Bukhari Vladimir Bytev Giacomo Cacciapaglia Hua Cai Wenyong Cai Wujun Cai Yijian Cai Yizhou Cai Yuchen Cai Haiying Cai Huacheng Cai Lorenzo Calibbi Junsong Cang Guofu Cao Jianshe Cao Antoine Chance Xuejun Chang Yue Chang Zhe Chang Xinyuan Chang Wei Chao Auttakit Chatrabhuti Yimin Che Yuzhi Che Bin Chen Danping Chen Fuqing Chen Fusan Chen Gang Chen Guoming Chen Hua-Xing Chen Huirun Chen Jinhui Chen Ji-Yuan Chen Kai Chen Mali Chen Mingjun Chen Mingshui Chen Ning Chen shanhong Chen shanzhen Chen shao-Long Chen shaomin Chen shiqiang Chen Tianlu Chen Wei Chen Xiang Chen Xiaoyu Chen Xin Chen Xun Chen Xurong Chen Ye Chen Ying Chen Yukai Chen Zelin Chen Zilin Chen Gang Chen Boping Chen Chunhui Chen Hok Chuen Cheng Huajie Cheng shan Cheng Tongguang Cheng Yunlong Chi Pietro Chimenti Wen Han Chiu Guk Cho Ming-Chung Chu Xiaotong Chu Ziliang Chu Guglielmo Coloretti Andreas Crivellin Hanhua Cui Xiaohao Cui Zhaoyuan Cui Brunella D'Anzi ling-Yun Dai Xinchen Dai Xuwen Dai Antonio De Maria Nicola De Filippis Christophe De La Taille Francesca De Mori Chiara De sio Elisa Del Core shuangxue Deng Wei-Tian Deng Zhi Deng Ziyan Deng Bhupal Dev Tang Dewen Biagio Di Micco Ran Ding siqin Dingl Yadong Ding Haiyi Dong Jianin AGH university of science and technology Faculty of Physics and Applied Computer ScienceKrakow Anhui university HefeiAnhui applied physics Department Federal Urdu University of ArtsScience and TechnologyIslamabad Banaras Hindu university Institute of ScienceVaranasi Bandirma Onyedi Eylul university Bandirma Beihang university Beijing beijing Computational science Research Center Beijing beijing Conveyi LTD Beijing beijing Glass Research institute Co. LtdBeijing beijing institute of technology Beijing beijing Normal university Beijing Brown university ProvidenceRI Budker institute of Nuclear physics Novosibirsk Cadi Ayyad university Laboratory of Fundamental and Applied PhysicsPolydisciplinary FacultyMarrakech Cairo university Giza Center for Theory and Computation National Tsing Hua UniversityHsinchu Central china Normal university WuhanHubei Central south university ChangshaHunan Centro de Fisica Teorica de Particulas Universidade Tecnica de LisboaLisboa Chengdu Kaitengsifang Digital Radio&TV Equipment Co. LtdChengduSichuan china academy of engineering physics MianyangSichuan china building materials academy Beijing china Center of Advanced science and technology Beijing china institute of Atomic Energy Beijing china Jiliang university HangzhouZhejiang china Nuclear Instrument CO. LTDBeijing china university of Geosciences Beijing china university of Geosciences WuhanHubei china university of Mining and technology XuzhouJiangsu china West Normal university NanchongSichuan Chongqing university Department of PhysicsChongqing Chulalongkorn university Bangkok City university of Hong Kong Department of PhysicsHong Kong Cockcroft institute Daresbury Cornell university IthacaNY CPPM(AMU/IPhU&CNRs/INP) Marseille Dalian Minzu university DalianLiaoning Dalian university of technology Department of PhysicsDalian Deutsches Elektronen-synchrotron DEsY Hamburg Dzhelepov Laboratory of Nuclear Problems of Joint institute for Nuclear Research(DLNP JINR) Dubna East china university Of science And Technol

The Circular Electron Positron Collider(CEPC)is a large scientific project initiated and hosted by china,fostered through extensive collaboration with international *** complex comprises four accelerators:a 30 GeV linac,a 1.1 GeV Damping Ring,a Booster capable of achieving energies up to 180 GeV,and a Collider operating at varying energy modes(Z,W,H,and tt).The linac and Damping Ring are situated on the surface,while the subterranean Booster and Collider are housed in a 100 km circumference underground tunnel,strategically accommodating future expansion with provisions for a potential super Proton Proton Collider(sPPC).The CEPC primarily serves as a Higgs *** its baseline design with synchrotron radiation(sR)power of 30 MW per beam,it can achieve a luminosity of 5×10^(34)cm^(-2.s^(-1)per interaction point(IP),resulting in an integrated luminosity of 13 ab^(-1)for two IPs over a decade,producing 2.6 million Higgs *** the sR power to 50 MW per beam expands the CEPC's capability to generate 4.3 million Higgs bosons,facilitating precise measurements of Higgs coupling at sub-percent levels,exceeding the precision expected from the HL-LHC by an order of *** Technical Design Report(TDR)follows the Preliminary Conceptual Design Report(Pre-CDR,2015)and the Conceptual Design Report(CDR,2018),comprehensively detailing the machine's layout,performance metrics,physical design and analysis,technical systems design,R&D and prototyping efforts,and associated civil engineering ***,it includes a cost estimate and a preliminary construction timeline,establishing a framework for forthcoming engineering design phase and site selection *** is anticipated to begin around 2027-2028,pending government approval,with an estimated duration of 8 *** commencement of experiments and data collection could potentially be initiated in the mid-2030s.

关键词： initiated exceeding precise

Lentivirus vectors construction of siRNA targeting interference GPC3 gene and its biological effects on liver cancer cell lines Huh-7

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Asian Pacific Journal of Tropical Medicine 2014年第10期7卷 780-786页

作者： Chang-jiang Lei Chun Yao Qing-Yun Pan Hao-Cheng Long Lei li shu-Ping Zheng Cheng Zeng Jian-Bin huang Department of General surgery the Second Affiliated Hospital of Jianghan University(Wuhan City Fifth Hospital) Wuhan institute of Hematology the Second Affiliated Hospital of Jianghan University(Wuhan City Fifth Hospital) Integrated Department Branch of Jianqiao.Hanyang Affiliated Hospital of Wuhan University of Science and Technology Department of Pediatrics the Second Affiliated Hospital of Jianghan University(Wuhan City Fifth Hospital)

Objective:To build GPC3 gene short hairpin interference RNA(shRNA)slow virus *** expression of Huh-7 GPC3 gene in human liver cell line proliferation apoptosis and the effect of GPC3 gene influencing on liver cancer cell growth,and provide theoretical basis for genc therapy of liver ***:Hepatocellular carcinoma cell line Huh-7 wsa transfected by a RNA interference ***3 gene expression in a variety of liver cancer cell lines was detected by fluorescence quantitative *** GPC3 gene seqnences of small interfering RNA(siRNA)PGC-shRNA-GPC3 were *** expression cell linse of siRNA were screened and established with the heplp of liposomes(lipofectamine^(TM2000))as carrier transfcetion of human liver cell *** order to validate siRNA interference ***3 siRNA mRNA expression was detected after transfection by using RT-PCR and Western *** absorbance value of the cells of blank group,untransfection group and transfection group,the cell cycle and cell apoptosis were calculated,and effects of GPC3 gene nn Huh-7 cell proliferation and apoptosis were ***:In the liver cancer cell lines Huh-7 GPC3 gene showed high ***-shRNA-GPC3 recombinant plasmid was constructde successfully via sequencing *** recombinant plasmid transfected into liver cancer cell linse Huh-7can obviously inhibit GPC3 mRNA expression ***:The targeted GPC3 siRNA can effectively inhibit the expression of GPC3.

关键词： GPC3 gene slow virus carrier liver cancer cell lines RNA interference

Bifunctional Activated Carbon Ultrathin Fibers:Combining the Removal of VOCs and PM in One Material

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Advanced Fiber Materials 2023年第6期5卷 1934-1948页

作者： Haiyang Wang Di Zu Xinyu jiang Yong Xu Zhiwen Cui Peng Du Zekun Cheng Ziwei li Lei li Chong Yang Xiaopeng Bai Baopu Zhang Yiqian Zhou Kuangyu Wang Bohan li Zhenghong huang lihao Zhao Bo li Hui Wu state Key Lab of New Ceramics and Fine Processing School of Materials Science and EngineeringTsinghua UniversityBeijing 100084People’s Republic of China institute of Materials Research Tsinghua Shenzhen International Graduate SchoolShenzhen 518055People’s Republic of China applied Mechanics Laboratory Department of Engineering MechanicsTsinghua UniversityBeijing 100084People’s Republic of China beijing Tongfang Envir-Clean technology Co. LTDBeijing 100083People’s Republic of China

Volatile organic compounds(VOCs)and particulate matter(PM)are both frequently present in air as contaminants,posing serious health and environmental *** current filtration of VOCs utilizes entirely different materials compared with PM filtration,adding complexity to air cleaning ***,we design a pitch-based activated carbon ultrathin fibers(PACUFs)for bifunctional air *** PACUFs,with fiber diameter of∼1.2μm and specific surface area of 2341 m^(2) g^(−1),provide both high VOCs adsorption capacity(∼706 mg g^(−1))and excellent efficiency of∼97% PM_(0.3) filtration with low pressure *** contrast,traditional activated carbon fibers exhibit VOCs adsorption capacity of∼448 mg g^(−1) and PM_(0.3) removal efficiency of only∼36%at an equal area density of∼190 g m^(−2).theoretical investigations reveal the filtration mechanism of the high-performance bifunctional fibrous PACUFs,considering full advantages of the high surface area,small pore size,and significant micropore volume.

关键词： Air pollutants Activated carbon fiber Melt-blown spinning Bifunctional purification

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泵喷推进器水动力及流场特性研究综述

力学学报 2022年第4期54卷 829-843,I0001页

作者：李晗黄桥高潘光董新国西北工业大学航海学院西安710072 中国船舶及海洋工程设计研究院喷水推进技术重点实验室上海200011

泵喷推进器(简称泵喷)是一种多部件组合式水动力推进装置,由导管及导管内的旋转叶栅(转子)和静止叶栅(定子)组成,具有高临界航速、重载、高效率和低辐射噪声等特点,最早于20世纪中叶在国外出现,初期主要用于鱼雷推进,后拓展到潜艇推进,... 详细信息

泵喷推进器(简称泵喷)是一种多部件组合式水动力推进装置,由导管及导管内的旋转叶栅(转子)和静止叶栅(定子)组成,具有高临界航速、重载、高效率和低辐射噪声等特点,最早于20世纪中叶在国外出现,初期主要用于鱼雷推进,后拓展到潜艇推进,目前国外潜艇、高速鱼雷普遍采用泵喷推进,国内外泵喷的研究和应用存在巨大差异.近年来,因国外技术封锁、潜艇装备发展需求和拓展泵喷应用等,泵喷推进技术成为水下装备领域的研究热点.泵喷各部件间流场相互作用复杂、与航行体耦合程度高,其应用和推广需要对其水动力和流场特性的深入认识.本文介绍了泵喷的基本构成和特点,按方向和目的总结了泵喷的水动力和流场特性研究现状.从水动力特性的角度,介绍了泵喷推进器的推进特性、预报方法和水动力变化规律.从流场特性角度,介绍了间隙流场、转-定子干扰流场和尾流场等.分析并总结了泵喷水动力和流场特性的研究以及实际应用中所面临的主要挑战,提出对未来研究的展望.

关键词：泵喷推进器水动力流场特性