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Transferrin and Its Isoforms from Normal Human Serum Revealed by Several Analytical Techniques

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Chemical Research in Chinese Universities 2008年第1期24卷 84-91页

作者： ZHUO Hui-qin huang he-qing JIN Hong-wei huang Lin Key Laboratory of MOE for Cell Biology and Tumor Cell Engineering School of Life Sciences State Key Laboratory of Marine Environmental Science College of Oceanography and Environmental Science The Key Laboratory of Chemical Biology of Fujian Province College of Chemistry ＆ Chemical Engineering Xiamen Center for Clinical Laboratory Zhongshan Hospital Xiamen University Xiamen 361005 P. R. China

Transferrin（TF） and its isoforms have been widely reported via various analytical techniques, including a noticeable increased number of isoforms with low content of sialic acid（asialo-, monosialo-, and disialo-transferrin） and asialo-TF as well as disialo-TF, with one or several oligosaccharides released in human serum transferrin（hTf）. here, hTf has been purified by native gradient polyacrylamide gel electrophoresis（PAGEso） before use. The hTf extracted with the electron-transfer approach showed a single subunit band（77.1 Da） in the SDS-PAGE gel, but it exhibited two bands in the native and denatured isoelectric focusing（IEF） gels, namely, hTf-2Fe^3＋ and apo-hTf, without finding any other transferrin isoforms. A reversed phase HPLC（RP-HPLC） equipped with a C18 column effectively separated hTf and its polymers and combined off-line techniques, including peptide mass fingerprinting（PMF）, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry（MALDI-TOF-MS） and database search, and identified the high homology among hTf, apo-hTf, and their isoforms. Moreover, the elution solution consisting of acetonitrile and formic acid could easily denature both hTf and apo-hTf to form various isoforms during separation with HPLC, indicating that chemical factors lead to the formation of various isoforms in transferrin, artificially, during extraction and separation. The authors claimed that only two transferrin isoforms existed in the NHS, namely, hTf-2Fe^3＋ and apo-hTf, which could be employed in biomarkers, to distinguish the healthy population from many disease sufferers, such as, carbohydrate-deficient transferrin（CDT）

关键词： Human serum transferrin RP-HPLC MALDI-TOF-MS PMF Biomarker

Synthesis for Spatially Interconnected Systems with Distributed Output Feedback Controllers

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自动化学报 2009年第8期35卷 1128-1135页

作者： huang huang WU qing-he LI Hui Department of Automation Beijing Institute of Technology Bei- jing 100081 P.R. China

This paper considers the design of distributed control architecture for spatially interconnected systems that are composed of several similar interconnected sub-units. Each sub-unit is a linear continuous time system and directly interacts with its nearest neighbors. This class of systems exists in several applications such as automated highway systems, power systems, and computer networks. Hybrid Lyapunov criterion and the hybrid real bounded lemma are derived to determine the stability and H∞ performance of the overall system. In order to stabilize this class of systems, distributed dynamic output feedback controllers are considered, and tractable linear matrix inequality (LMI)-based algorithms for the derivation of distributed controllers are presented. The change variable approach is introduced in the LMI-based algorithms due to its higher efficiency and numerically stable implementation than the elimination algorithm as introduced in previous works. It is shown through a numerical example that the distributed H∞ controllers developed in this article are superior to decentralized controllers in several aspects.

关键词：空间互联系统自动化系统反馈控制器稳定性

Studies on Chem icalCom position ofIron-m olybdenum Cofactor fromNitrogenase by Fluorescence Analysis

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Chemical Research in Chinese Universities 1999年第3期15卷 286-293页

作者： ZHANG Feng-zhang XU Liang-shu huang he-qing LONG Min-nan QIU Xue-hui CheN Zhong Department of Biology Xiamen UniversityXiamen 361005P.R.China

The iron molybdenum cofactor(FeMoco) of nitrogenase MoFe protein from Azotobacter vinelandii OP was extracted by N methylformamide(NMF). The effects of FeMoco(in NMF) on electronic spectrum and fluorescence intensity of fluorescein dimercury acetate(FDMA)(in 1 mol/L NaOH) were investigated by fluorophotometric titrations and compared with those of (NH 4) 2MoS 4 and complexes of (NH 4) 2MoS 4 with Na 2S or Na 2S 2 or (NH 4) 2S x on the relative properties of FDMA. It was found that the electronic spectrum of FDMA displayed hypsochromic shift(17 nm) in the presence of FeMoco just like that in the presence of other inorganic sulfides and that the titration curve for the quench of FDMA with FeMoco is very similar to that for the quench of FDMA with complex of (NH 4) 2MoS 4 with Na 2S 2(mole ratio is 1∶3). The results showed that FeMoco(N) probably contained S—S bonds and its structure was found to be changed compared with Kim Rees structural model. This change should profit the formation of the polymer.

关键词： Nitrogenase Iron molybdenum cofactor Fluorophotometric titration Structural change

Effect of Astragalus membranaceus injection on the activity of the intestinal mucosal mast cells after hemorrhagic shock-reperfusion in rats

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Chinese Medical Journal 2006年第22期119卷 1892-1898页

作者： GAN Xiao-liang heI Zi-qing huang he-qing CheN Li-xin LI Shang-rong CAI Jun Department of Anesthesiology Third Affiliated Hospital SunYat-sen University Guangzhou 510630 China School of Pharmaceutical Sciences Sun Yat-sen UniversityGuangzhou 510080 China

Background The mechanism of mucosal damage induced by ischemia-reperfusion （IR） after hemorrhagic shock is complex; mast cells （MC） degranulation is associated with the mucosal damage. Astragalus membranaceus can protect intestinal mucosa against intestinal oxidative damage after hemorrhagic shock, and some antioxidant agents could prevent MC against degranulation. This study aimed to observe the effects of astragalus membranaceus injection on the activity of intestinal mucosal mast cells （IMMC） after hemorrhage shock-reperfusion in rats Methods Thirty-two Wistar rats were randomly divided into the normal group, model group, low dosage group, （treated with Astragalus membranacaus injection, 10 g crude medication/kg） and high dosage group （treated with Astragalus membranacaus injection, 20 g crude medication/kg）. The rat model of hemorrhagic shock-reperfusion was induced by hemorrhage for 60 minutes followed by 90 minutes of reperfusion. The animals were administrated with 3 ml of the test drug solution before reperfusion. At the end of study, intestinal pathology, ultrastructure of IMMC, and expression of tryptase were assayed. The levels of malondisldehyde （MDA）, TNF-α, histamine, and superoxide dismutase （SOD） activity in intestine were detected, and the number of IMMC was counted. Results The Chiu＇s score of the rats in the model group was higher than in other groups （P〈0.01）. The Chiu＇s score in the high dosage group was higher than that in the low dosage group （P〈0.05）. hemorrhage-reperfusion induced IMMC degranulation： Astragalus membranaceus injection attenuated this degranulation. Expression of tryptase and the number of IMMC in the model group increased compared with the other groups （P〈0.01） and was significantly reduced by the treatments of Astragalus membranaceus injection at both doses. There was no significant difference between the two treatment groups （P〉0.05）. MDA content and concentration of TNF-α in the model group were higher than that in the oth

关键词： Astragalus membranaceus injection hemorrhage shock reperfusion intestinal mucosal mast cells histamine tumor necrosis factor-α

Design and Simulation of a Electrorheological(ER) Control High Voltage Power Supply

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International Journal of Plant Engineering and Management 2009年第3期14卷 142-146页

作者： ZHU Shi-sha huang he-qing BAI Quan Institute of Mechanical Engineering Xiangtan University Xiangtan 411105 P. R. China

Few applications with electrorheological （ER） fluids have been found in industry. One reason is high voltage power supplies cannot meet the requirement for ER effect. In this paper, an ER control high voltage power supply for engineering application was designed which reduced power wastage by adopting soft switch technology; lessened its volume to satisfy micromation when adopting the planar transformer and improved its response character- istic by choosing an assistant discharging circuit. Simultaneously, a simulation analysis has been carried out. As results, the output voltage of this high voltage power supply is 5 kV, and the voltage can be continuously regulated and the voltage ripple is only 0. 7 %, so the requirement of stability is also achieved.

关键词： ER control high voltage power supply application design soft switch technology simulation

棕色固氮菌细菌铁蛋白捕获能力、稳定性和亚基相互作用的强度

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分析化学 2008年第8期36卷 1045-1050页

作者：黄琳陈旭罗联忠林青黄河清厦门大学生命科学学院细胞生物学与肿瘤细胞工程教育部重点实验室

选用柱层析、电泳和反相高效液相色谱（RP-HPLC）技术制备质谱纯棕色固氮菌细菌铁蛋白（Bacteri-al ferritin ofAzotobacter vinelandii,AVBF）,并采用释放铁动力学和肽质量指纹图谱（Peptide mass fingerprint-ing,PMF）技术分别鉴定A... 详细信息

选用柱层析、电泳和反相高效液相色谱（RP-HPLC）技术制备质谱纯棕色固氮菌细菌铁蛋白（Bacteri-al ferritin ofAzotobacter vinelandii,AVBF）,并采用释放铁动力学和肽质量指纹图谱（Peptide mass fingerprint-ing,PMF）技术分别鉴定AVBF。基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）和电泳技术揭示AVBF亚基之间相互作用强度、稳定性和聚合态。AVBF可直接捕获有机小分子亚甲蓝（MB）,其捕获率为15.0±2.0MB/AVBF,认为介于AVBF亚基单体之间的血红素参与捕获MB。较高浓度（40%;0%）的乙腈和丙酮均能使AVBF和鲨鱼肝铁蛋白（Liver ferritin of shark,SLF）释放不稳定亚基,但在较低浓度（20%;0%）的乙腈条件下,却需要借助来源于质谱仪的激光才能使AVBF或SLF释放不稳定亚基,并供质谱分析。AVBF亚基之间的相互作用强度明显低于SLF。铁蛋白亚基之间的相互作用强度高低与铁蛋白执行释放和储存铁的速率有关。

关键词：细菌铁蛋白基质辅助激光解析电离飞行时间质谱电泳亚基相互作用稳定性捕获能力

Emodin inhibits dietary induced atherosclerosis by antioxidation and regulation of the sphingomyelin pathway in rabbits

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Chinese Medical Journal 2006年第10期119卷 868-870页

作者： heI Zi-qing huang he-qing TAN Hong-mei LIU Pei-qing ZHAO Ling-zhi CheN Shao-rui huang Wen-ge CheN Feng-ying GUO Fen-fen Department of Anesthesiology Third Affiliated Hospital SunYat-sen University Guangzhou 510630 China School of Pharmaceutical Sciences Sun Yat-sen UniversityGuangzhou 510080 China Zhongshan Medical College Sun Yat-sen University Guangzhou510080 China

Emodin is an important component of rhubarb, a traditional Chinese herb. Previous studies in vitro showed that emodin inhibited the proliferation of human vascular smooth muscle cells, indicating the possible inhibitive effect of emodin on atherosclerosis. The present research evaluated the effect of emodin on the formation of atherosclerotic lesion induced by high cholesterol and fat diet in rabbits and the mechanism of this effect.

关键词： SIGNAL-TRANSDUCTION CERAMIDE

MALDI-TOF MS和电子光谱技术研究海兔肝铁蛋白亚基电离和释放铁动力学特性

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分析化学 2007年第12期35卷 1745-1750页

作者：黄琳陈旭林青朱斌琳黄河清化学生物学福建省重点实验室厦门361005 厦门大学生命科学学院细胞生物学与肿瘤细胞工程教育部重点实验室厦门大学生命科学学院细胞生物学与肿瘤细胞工程教育部重点实验室厦门大学化学化工学院固体表面物理化学国家重点实验室

选用肽质量指纹谱(peptide mass fingerprint,PMF)技术鉴定质谱纯海兔肝铁蛋白(liver ferritin ofAplysia,ALF)。来源于基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)仪中的激光和基质芥子酸协同解吸海兔肝铁蛋白(ALF)为带双电荷、... 详细信息

选用肽质量指纹谱(peptide mass fingerprint,PMF)技术鉴定质谱纯海兔肝铁蛋白(liver ferritin ofAplysia,ALF)。来源于基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)仪中的激光和基质芥子酸协同解吸海兔肝铁蛋白(ALF)为带双电荷、单电荷[M+H]+和二聚体的亚基离子,并可供质谱分析。ALF亚基的质荷比m/z分别为9784.03[M+2H]2+、19678.42[M+H]+和39387.80[2M+H]+,其中亚基分子量[M+H]+略小于鲨鱼肝铁蛋白(liver ferritin of shark,SLF)。在弱碱介质(pH8.0)条件下,电子光谱技术研究指出,抗坏血酸以1/2级反应方式参与ALF释放铁全过程,同时又使ALF以一级反应动力学方式释放铁,呈现两种不同的速率。推测这一异常现象可能与ALF含低铁量、亚基调节能力和海兔的进化地位有关。

关键词：基质辅助激光解吸电离飞行时间质谱亚基解离释放铁动力学进化

茶多酚锰-壳聚糖微球的制备、控释和诱导肝癌细胞凋亡的研究

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高等学校化学学报 2008年第8期29卷 1592-1597页

作者：黄河宁李程谢笠升黄河清厦门大学生命科学学院细胞生物学与肿瘤细胞工程教育部重点实验室

选用壳聚糖为微米粒包被材料,制备茶多酚锰(Tea Polyphenol Manganese,TPMn)-壳聚糖微球.用荧光显微技术研究了TPMn-壳聚糖微球的荧光特性,用扫描和透射电子显微镜证实TPMn-壳聚糖微球尺寸和分布规律.RP-HPLC定量分析TPMn-壳聚糖微球包... 详细信息

选用壳聚糖为微米粒包被材料,制备茶多酚锰(Tea Polyphenol Manganese,TPMn)-壳聚糖微球.用荧光显微技术研究了TPMn-壳聚糖微球的荧光特性,用扫描和透射电子显微镜证实TPMn-壳聚糖微球尺寸和分布规律.RP-HPLC定量分析TPMn-壳聚糖微球包封率为68%,符合微米级微粒控释药物包封率的要求.动力学研究结果表明,茶多酚(TP)-壳聚糖和TPMn-壳聚糖的微球均有控释TP的能力,控释时间高达40h以上,但前者释放速率稍快于后者.TPMn和TPMn-壳聚糖微球均能诱导肝癌细胞凋亡,但TPMn-壳聚糖微球诱导肿瘤细胞的凋亡速率稍高于TPM.实验结果证实,以TPMn-壳聚糖微球方式控释TPMn有利于提高诱导肿瘤细胞凋亡速率.TPMn-壳聚糖微球具有研发成注射型抗肿瘤新药的可能性.

关键词：茶多酚锰-壳聚糖微球理化特性控释肝癌细胞凋亡