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Molecular and morphological characterization of stunt nematodes of wheat, maize, and rice in the savannahs of northern Nigeria

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Journal of Integrative Agriculture 2022年第2期21卷 586-595页

作者： Sulaiman ABDULSALAM PENG Huan LIU Shi-ming HUANG Wen-kun KONG Ling-an PENG De-liang State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection Chinese Academy of Agricultural SciencesBeijing 100193P.R.China Division of Agricultural Colleges Department of Crop ProtectionAhmadu Bello UniversityZaria 810107Nigeria

Stunt nematodes(Tylenchorhynchus spp.)are obligate migratory root ecto-parasitic nematodes found in the fields of many cultivated crops.These nematodes,with phyto-sanitary potential,are frequently ignored or misdiagnosed as pests,and this may pose a threat to food security.The accuracy of its identification based on a morphological approach has been challenged recently,due to the overlapping of the morphological and morphometric characters of the species.Consequently,the objective of this study is to identify and characterize stunt nematodes present in 54 fields cultivated with cereal crops(wheat,maize and rice)in the savannahs of northern Nigeria,using integrative taxonomy and molecular approaches.The molecular and morphological studies identified and confirmed the presence of T.annulatus as the occurring specie in the savannahs of northern Nigeria.The phylogenetic analysis was carried out using the internal transcribed spacer(ITS)and 28S genes of ribosomal DNA further confirmed the presence of T.annulatus.The first molecular characterization and sequences of the ITS and 28S rDNA gene for T.annulatus from Nigeria were provided by this research.Also,according to our literature search,this is the first report on T.annulatus in wheat,maize and rice in the savannahs of northern Nigeria.Further study to test the pathogenicity of the parasitic nematode species found in this survey is recommended for the prioritization and development of efficient management strategies.

关键词： plant-parasitic nematodes food security Tylenchorhynchus annulatus ITS phylogeny

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Mask Distillation Network for Conjunctival Hyperemia Severity Classification

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Machine Intelligence Research 2023年第6期20卷 909-922页

作者： Mingchao Li kun huang Xiao Ma Yuexuan Wang wen Fan Qiang Chen School of Computer Science and Engineering Nanjing University of Science and TechnologyNanjing210094China Department of Ophthalmology The First Affiliated Hospital with Nanjing Medical UniversityNanjing210029China

To achieve automatic,fast and accurate severity classification of bulbar conjunctival hyperemia severity,we proposed a novel prior knowledge-based framework called mask distillation network(MDN).The proposed MDN consists of a segmentation network and a classification network with teacher-student branches.The segmentation network is used to generate a bulbar conjunctival mask and the classification network divides the severity of bulbar conjunctival hyperemia into four grades.In the classification network,we feed the original image and the image with the bulbar conjunctival mask into the student and teacher branches respectively,and an attention consistency loss and a classification consistency loss are used to keep a similar learning mode for these two branches.This design of“different input but same output”,named mask distillation(MD),aims to introduce the regional prior knowledge that“bulbar conjunctival hyperemia severity classification is only related to the bulbar conjunctiva region”.Extensive experiments on 5117 anterior segment images have proven the effectiveness of mask distillation technology:1)The accuracy of the MDN student branch is 3.5%higher than that of a single optimal baseline network and 2%higher than that of the baseline network combination.2)In the test phase,only the student branch is needed,and no additional segmentation network is required.The framework only takes 0.003 s to classify a single image,achieving the fastest speed in all the methods we compared.3)Compared with a single baseline network,the attention of both teacher and student branches in the MDN has been intuitively improved.

关键词： Mask distillation(MD) conjunctiva hyperemia attention mechanism severity classification deep learning

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Separation of Pd and Pt from highly acidic leach liquor of spent automobile catalysts with monothio-Cyanex 272 and trioctylamine

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International Journal of Minerals,Metallurgy and Materials 2023年第5期30卷 877-885页

作者： Junlian Wang Lu Liu wen Xu Hui Liu Guodong Xu kun huang Fengshan Yu Guoyong huang School of Civil and Resource Engineering University of Science and Technology BeijingBeijing 100083China Key Laboratory of Ministry of Education for Efficient Mining and Safety of Metal Mines Beijing 100083China State Key Laboratory of Heavy Oil Processing College of Chemical EngineeringChina University of Petroleum-BeijingBeijing 102249China School of Metallurgical and Ecological Engineering University of Science and Technology BeijingBeijing 100083China Jiangxi Province Han’s Precious Metals Co. Ltd.Shangrao 335500China

Platinum group metals(PGMs),especially Pd,Pt,and Rh,have drawn great attention due to their unique features.Direct separation of Pd and Pt from highly acidic automobile catalyst leach liquors is disturbed by various factors.This work investigates the effect of various parameters including the acidity,extractant concentration,phase ratio A/O,and diluents on the Pd and Pt extraction and their stripping behaviors.The results show that the Pd and Pt are successfully separated from simulated leach liquor of spent automobile catalysts with monothioCyanex 272 and trioctylamine(TOA).Monothio-Cyanex 272 shows strong extractability and specific selectivity for Pd,and only one single stage is needed to recover more than 99.9% of Pd,leaving behind all the Pt,Rh,and base metals of Fe,Mg,Ce,Ni,Cu,and Co in the raffinate.The loaded Pd is efficiently stripped by acidic thiourea solutions.TOA shows strong extractability for Pt and Fe at acidity of 6 mol·L^(–1) HCl.More than 99.9% of Pt and all of the Fe are extracted into the organic phase after two stages of countercurrent extraction.Diluted HCl easily scrubs the loaded base metals(Fe,Cu,and Co).The loaded Pt is efficiently stripped by 1.0 mol·L^(–1) thiourea and 0.05–0.1 mol·L^(–1) Na OH solutions.Monothio-Cyanex 272 and TOA can realize the separation of Pd and Pt from highly acidic leach liquor of spent automobile catalysts.

关键词： platinum group metals spent automobile catalysts extraction separation high chloride media

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Effect of Aspergillus niger NBC001 on the soybean rhizosphere microbial community in a soybean cyst nematode-infested field

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Journal of Integrative Agriculture 2021年第12期20卷 3230-3239页

作者： JIN Na LIU Shi-ming PENG Huan HUANG Wen-kun KONG Ling-an PENG De-liang Department of Horticulture Beijing Vocational College of AgricultureBeijing 102442P.R.China State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China

Soybean cyst nematode(SCN,Heterodera glycines Ichinohe)is one of the most important pests causing considerable damage to soybean(Glycine max(L.)Merr.)around the world.Biocontrol provides a strategy for sustainable nematode control.Previously,Aspergillus niger NBC001 was isolated from the cysts Heterodera spp.and able to control H.glycines and promote the growth of soybean in a pot experiment.In this study,the effects of NBC001 on H.glycines density and on the soybean rhizosphere microbial community in a soybean cyst nematode-infested field were studied.The results showed that NBC001 could suppress H.glycines by 31.7%in the field.High-throughput sequencing analysis showed that NBC001 had no significant influence on soybean rhizosphere microbial community structure,indicating that seed coatdressing with the concentrated culture filtrate of NBC001 was safe for the soil ecological environment.In addition,high-throughput sequencing results demonstrated that at 10 days post transplantation,NBC001 increased the abundances of Actinobacteria and Acidobacteria,but decreased the abundances of Bacteroidetes and Gemmatimonadetes at the phylum level.Meanwhile,the abundances of Phyllobacterium,Ralstonia and H16 were increased,while the abundances of Adhaeribacter,Gemmatimonas,Sphingomonas,Flavisolibacter\Nere suppressed by application of NBC001.However,at 90 days post transplantation,NBC001 only increased the abundances oi Aeromicrobium and RB41 whereas it decreased the abundance of H16.The results indicated that application of NBC001 increased the relative abundances of the beneficial microorganisms such as Actinobacteria,Acidobacteria,Aeromicrobium and Phyllobacterium in the soil.In summary,NBC001 is an eco-friendly biocontrol agent for H.glycines control.

关键词： Aspergillus niger NBC001 biocontrol Heterodera glycines high-throughput sequencing microbial community

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Consumers' perceptions on GM food safety in urban China

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Journal of Integrative Agriculture 2015年第11期14卷 2391-2400页

作者： huang Ji-kun PENG Bo-wen Center for Chinese Agricultural Policy Institute of Geographical Sciences and Natural Resources ResearchChinese Academy of Sciences University of Chinese Academy of Sciences

The debate about the safety of genetically modified （GM） food has attracted public attention in concurrence with the rapid development of agricultural biotechnology. This paper examines the consumers＇ perceptions on the safety of GM food in China. Based on a unique survey dataset, this study shows that consumers in urban China have significantly changed their perceptions on GM food safety. The percentage of consumers who perceived such food as unsafe for consumption increased by more than 30% in the 2002-2012 period. Approximately half of the consumers did not have an opinion on this issue. Major shifts have been occurred after 2010, likely because of the increasing influence of negative media reports on GM technology in recent years. Several individual and household＇s characteristics are shown to significantly affect consumers＇ perceptions on GM food safety, such as gender, education, food allergy experience and resident city size. The paper concludes with policy implications.

关键词： food safety GM food urban China consumers perception

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Resistance of barley varieties to Heterodera avenae in the Qinghai–Tibet Plateau,China

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Journal of Integrative Agriculture 2022年第5期21卷 1401-1413页

作者： YAN Jia-hui JIA Jian-ping JIANG Li-ling PENG De-liang LIU Shi-ming HOU Sheng-ying YU Jing-wen LI Hui-xia HUANG Wen-kun Key Laboratory of Agricultural Integrated Pest Management Qinghai Academy of Agriculture and Forestry SciencesXining 810016P.R.China State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection Chinese Academy of Agricultural SciencesBeijing 100193P.R.China College of Plant Protection Gansu Agricultural UniversityBiocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu ProvinceLanzhou 730070P.R.China

The cereal cyst nematode,Heterodera avenae,is one of the most economically important pathogens impacting the worldwide production of cereals and is widely distributed in more than 16 regions in China.The present study used the numbers of nematodes inside the plant roots to evaluate the resistance/susceptibility of different subpopulations of barley Hordeum vulgare(QH2R,QH6R and TB2R)to H.avenae under field and pot conditions.Nematode development in two highly resistant varieties was also evaluated by in vivo experiment and microscopic observation.Analyses of 186selected varieties showed the numbers of susceptible varieties identified with the number of females/cysts per plant(NFP)method were significantly higher than those identified with the Pf/Pi ratio(PPR)method,which indicated that the NFP method rather than the PPR method is more reliable to evaluate the resistance of barley.The field and pot experiment results indicated that the QH2R subpopulation had lower females/cysts numbers than QH6R and TB2R subpopulations,and eight HR varieties(Sunong 7617,Sunong 7635,Dongyuan 87-14,Rudong 14-46,Rudong 87-57,Rudong 87-8-45,Rudong 88-14-2,and Rudong 88-67-1)were identified in QH2R,with the NFP numbers below 4.2.Further microscopic observation of nematode development suggested that H.avenae often penetrated less into highly resistant varieties(Sunong 7635 and Dongyuan 87-14)and more frequently failed to develop into females than the susceptible barleys.The promising resistant varieties identified in the present research might be helpful for breeders to develop CCN-resistant cultivars and control H.avenae populations effectively at low costs.

关键词： Heterodera avenae barley varieties resistance evaluation number of females/cysts per plant(NFP)

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Assaying the potential of twenty-one legume plants in Medicago truncatula and M. sativa for candidate model plants for investigation the interactions with Heterodera glycines

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Journal of Integrative Agriculture 2016年第3期15卷 702-704页

作者： KONG Ling-an WU Du-qing HUANG Wen-kun PENG Huan HE wen-ting PENG De-liang State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences

Soybean cyst nematode Heterodera glycines is one of the most serious soil-borne pathogens in soybean production. However, the researches were limited in China due to lack of an effective pathosystem. In this study, we screened 21 legume Medicago plants in both Medicago truncatula and Medicago sativa to obtain candidate model plants for establishing a new pathosystem for legume-H. glycines interactions. The nematode infection of tested plants was assayed with Race 3 and 4 respectively, which were two dominant H. glycines inbred races in China soybean producing areas. The results showed that the model legume plant M. truncatula A17 failed to allow Race 3 of H. glycines to complete its life cycle, in contrast, it provided the Race 4 population to form several cyst nematodes, however, the female index（FI） value was approximately 1.6. Three M. sativa cultivars, including Xunlu, Aergangjin and Junren, provided either Race 3 or 4 of H. glycines to develop into mature cysts with their FI value below 5 as well. Our results demonstrated that legume plants in both M. truncatula and M. sativa were not likely to be a model plant for H. glycines because of an extreme high resistance.

关键词： soybean cyst nematode alfalfa life cycle female index pathosystem

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A fragment of a 70-kDa Heterodera glycines heat shock protein(HgHSP70)interacts with soybean cyst nematode-resistant GmSHMT08

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Journal of Integrative Agriculture 2022年第10期21卷 2973-2983页

作者： LIU Zhi ZHANG Liu-ping ZHAO Jie JIAN Jin-zhuo PENG Huan HUANG Wen-kun KONG Ling-an PENG De-liang LIU Shi-ming State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China MARA–CABI Joint Laboratory for Bio-safety Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China

Soybean cyst nematode(SCN)Heterodera glycines is considered as the major constraint to soybean production.Gm SHMT08 at Rhg4 locus on chromosome 08,encoding a serine hydroxylmethyltransferase,is a major gene underlying resistance against H.glycines in Peking-type soybeans.However,the molecular mechanism underpinning this resistance is less well characterized,and whether Gm SHMT08 could interact with proteins in H.glycines remains unclear.In this study,yeast two-hybrid screening was conducted using Gm SHMT08 as a bait protein,and a fragment of a 70-kDa heat shock protein(Hg HSP70)was screened from H.glycines that exhibited interaction with Gm SHMT08.This interaction was verified by both GST pull-down and bimolecular fluorescence complementation assays.Our finding reveals Hg HSP70 could be applied as a potential candidate gene for further exploring the mechanism on Gm SHMT08-mediated resistance against SCN H.glycines.

关键词： soybean cyst nematode Heterodera glycines GmSHMT08 HgHSP70 interaction resistance

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Regulation of a vacuolar proton-pumping P-ATPase MdPH5 by MdMYB73 and its role in malate accumulation and vacuolar acidification

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aBIOTECH 2023年第4期4卷 303-314页

作者： Xiao-Yu huang Ying Xiang Yu-wen Zhao Chu-kun Wang Jia-Hui Wang wen-Yan Wang Xiao-Long Liu Quan Sun Da-Gang Hu National Research Center for Apple Engineering and Technology Shandong Collaborative Innovation Center of Fruit and Vegetable Quality and Efficient ProductionCollege of Horticulture Science and EngineeringShandong Agricultural UniversityTai’an 271018ShandongChina

As the main organic acid in fruits,malate is produced in the cytoplasm and is then transported into the vacuole.It accumulates by vacuolar proton pumps,transporters,and channels,affecting the taste and flavor of fruits.Among the three types of proton pumps(V-ATPases,V-PPases,and P-ATPases),the P-ATPases play an important role in the transport of malate into vacuoles.In this study,the transcriptome data,collected at different stages after blooming and during storage,were analyzed and the results demonstrated that the expression of MdPH5,a vacuolar proton-pumping P-ATPase,was associated with both pre-and post-harvest malate contents.Moreover,MdPH5 is localized at the tonoplast and regulates malate accumulation and vacuolar pH.In addition,MdMYB73,an upstream MYB transcription factor of MdPH5,directly binds to its promoter,thereby transcriptionally activating its expression and enhancing its activity.In this way,MdMYB73 can also affect malate accumulation and vacuolar pH.Overall,this study clarifies how MdMYB73 and MdPH5 act to regulate vacuolar malate transport systems,thereby affecting malate accumulation and vacuolar pH.

关键词： Apple Malate accumulation P-ATPase MdPH5 MdMYB73

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Molecular Characterization and Functional Analysis of a New Acid Phosphatase Gene(Ha-acp1) from Heterodera avenae

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Journal of Integrative Agriculture 2014年第6期13卷 1303-1310页

作者： LIU Yan-ke HUANG Wen-kun LONG Hai-bo PENG Huan HE wen-ting PENG De-liang State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences

For sedentary endo-parasitic nematodes, parasitism genes encoding secretory protein expressed in the subventral glands cells always play an important role during the early parasitic process. A new acid phosphatase gene （Ha-acp1） expressed in the subventral glands of the cereal cyst nematode （Heterodera avenae） was cloned and the characteristics of the gene were analyzed. Results showed that the gene had a putative signal peptide for secretion and in situ hybridization showed that the transcripts of Ha-acp1 accumulated speciifcally in the subventral gland cells of H. avenae. Southern blot analysis suggested that Ha-acp1 belonged to a multigene family. RT-PCR analysis indicated that this transcription was strong at the pre-parasitic juveniles. Knocking down Ha-acp1 using RNA interference technology could reduce nematode infectivity by 50%, and suppress the development of cyst. Results indicated that Ha-acp1 could play an important role in destroying the defense system of host plants.

关键词： Heterodera avenae acid phosphatase RNA interference

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