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Qualitative and quantitative analysis of glucosinolates and nucleosides in Radix Isatidis by HPLC and liquid chromatography tandem mass spectrometry

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Acta Pharmaceutica Sinica B 2013年第5期3卷 337-344页

作者： Xiuming Wang Yuanyuan Xie xiaomei hu Youkang Li Ping hu Yiming Wang Qionglin Liang Guoan Luo School of Pharmacy East China University of Science and TechnologyShanghai 200237China Department of Chemistry Tsinghua UniversityBeijing 100084China

Multi-component fingerprinting and quantitation of the glucosinolates and nucleosides in samples of Radix Isatidis have been carried out using high-performance liquid chromatography with diode-array detection and electrospray ionization tandem mass spectrometry(HPLC–DAD–ESI/MS).Five nucleosides together with one glucosinolate were identified by comparing retention times,ultraviolet spectra,mass spectra and/or empirical molecular formulae of reference *** of these six compounds was carried out simultaneously by HPLC on a Phenomenex Luna C18 column using gradient elution with methanol and water and detection at 254 *** calibration curves were linear(r40.9994)within test *** of detection and quantitation were 0.33 ng and 2.50 ng on column,***-and inter-day precision(as relative standard deviation)for all analytes was o2.19%with recoveries in the range 99.6%–101.8%at three concentration *** validated method was successfully applied to fingerprinting and assay of 25 batches of Radix Isatidis sourced from different geographical regions of *** method is simple and reliable and has potential value in the quality control of Radix Isatidis.

关键词： Radix Isatidis Glucosinolate Nucleoside R,S-Goitrin Quality control HPLC–DAD–ESI/MS

Arsenic concentration in peripheral blood is correlated with efficacy of a Traditional Chinese Medicine regimen containing realgar for the treatment of myelodysplastic syndromes

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Journal of Traditional Chinese Medicine 2021年第4期41卷 630-635页

作者： WANG Dexiu XU Yonggang DU Yu WANG Hongzhi LI Liu hu xiaomei YANG Xiupeng MA Rou ZHOU Qingbing Department of Hematology Xiyuan HospitalChina Academy of Chinese Medical SciencesBeijing 100091China Institute of Geriatric Medicine Xiyuan HospitalChinese Academy of Chinese Medical SciencesBeijing 100091China

OBJECTIVE:To explore the relationship between the efficacy of realgar for the treatment of myelodysplastic syndromes with multilineage dysplasia(MDS-MLD)and arsenic concentration in the peripheral blood of ***:In this prospective study,a total of 50 MDS-MLD patients were treated with traditional Chinese drugs containing realgar for 3 months in Xiyuan Hospital from March 2018 to January *** blood examination as well as liver and kidney function were monitored before and after *** concentration of arsenic in the peripheral blood was measured using an atomic fluorescence spectrometer after *** correlation between clinical effect and arsenic concentration was analyzed by Spearman’s ***:The treatment response rate was 54%.Two patients(4%)achieved complete remission,50%(25 of 50)showed hematologic improvement,and 23 patients had stable disease(23%).No disease progression was *** concentration in the peripheral blood ranged from 14.60 to 85.96μg/*** efficacy was positively correlated with arsenic concentration(P<0.05).The incidence of mild adverse reactions was 16%.CONCLUSION:A relatively high concentration of arsenic in the peripheral blood may improve the clinical efficacy of realgar in MDS-MLD patients.

关键词： realgar arsenic myelodysplastic syndromes multiline dysplasia

Study on Genetic Diversity among Soybean in Heilongjiang Province

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Journal of Northeast Agricultural University(English Edition) 2006年第1期13卷 11-15页

作者： hu Guofu hu xiaomei CHANG Ying LIU Di hu Baozhong College of Life Science Northeast Agricultural University Harbin Heilongjiang 150030 PRC School of Life and Health Sciences Aston University Birmingham B4 7ET UK

Genetic characters of 10 soybean breeds in Heilongjiang Province were studied using RAPD technique. We calculated the genetic similarity and genetic distance, made the cluster analysis, and also studied the genetic relationship among the 10 breeds. We found 93 amplification segments by RAPE） analysis, in which there were 70 polymorphism segments, 75.26% of amplification segments, using 13 random primers, 11, 12, 5, 4, 7, 8, 3, 7, 2, 5, 10, 10, 9 amplificated bands were found. There were 7.15 bands per primer in average, The genetic similarity among the breeds ranged from 0.6848 to 0.9019, and the genetic distance ranged from 0.0981 to 0.3152. Genetic relationship between 90035 and 9674 was the closest, and between Heifeng 27 and 90035 was the farthest. Protein analysis revealed that the closest genetic relationship was between 90035 and 90024, and the farthest was between 1330 and Sui 4, and between 1330 and Heinong 39.

关键词： soybean RAPD protein genetic character

Construction of Clonal Growth Models in Trifolium repens

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Journal of Northeast Agricultural University(English Edition) 2009年第4期16卷 14-18页

作者： huBaozhong LI Fenglan hu xiaomei hu Guofu YUAN Qiang College of Life Sciences Northeast Agricultural University Harbin 150030 China

The suitability of models for describing the clonal growth of Trifolium repens population was discussed. The results showed that deterministic models were inadequate for describing its clonal growth, but the diffusion models and the randomwalk models suited for the clonal growth characteristics of the population. And it was found that random-walk models were better than diffusion models for describing a population in an environment with rich natural resources, and the latter was better in a poor environment.

关键词： Trifolium repens clonal growth model diffusion model random-walk model

中国式分权、文化非正式制度与环境污染关系的实证

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统计与决策 2018年第15期34卷 98-102页

作者：胡小梅长沙银行博士后科研流动站协作研发中心湖南财政经济学院财政金融学院

文章基于我国2000—2015年的省级面板数据,分别合成了中国式分权（环境分权和财政分权）与文化非正式制度的各项指标,并采用动态空间面板模型,实证检验了中国式分权、文化非正式制度及其互动机制,对环境污染的空间影响效应。结果表明：... 详细信息

文章基于我国2000—2015年的省级面板数据,分别合成了中国式分权（环境分权和财政分权）与文化非正式制度的各项指标,并采用动态空间面板模型,实证检验了中国式分权、文化非正式制度及其互动机制,对环境污染的空间影响效应。结果表明：我国环境污染存在一定的空间异质性特征;环境分权与文化非正式制度因素加剧了环境污染,而财政分权制度则有利于环境质量的优化,三者之间的交互作用对地区间的＂策略性＂减排行为具有重要影响。

关键词：中国式分权环境分权财政分权文化非正式制度环境污染

Optimizing a high-sensitivity NanoLuc-based bioluminescence system for in vivo evaluation of antimicrobial treatment

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mLife 2023年第4期2卷 462-478页

作者： Weilong Shang Zhen hu Mengyang Li Yuting Wang Yifan Rao Li Tan Juan Chen Xiaonan huang Lu Liu He Liu Zuwen Guo huagang Peng Yi Yang Qiwen hu Shu Li xiaomei hu Jiao Zou Xiancai Rao Department of Microbiology College of Basic Medical SciencesKey Laboratory of Microbial Engineering Under the Educational Committee in ChongqingArmy Medical University(Third Military Medical University)ChongqingChina Department of Microbiology School of MedicineChongqing UniversityChongqingChina Department of Emergency Medicine Xinqiao HospitalArmy Medical University(Third Military Medical University)ChongqingChina Department of Pharmacy Xinqiao HospitalArmy Medical University(Third Military Medical University)ChongqingChina Department of Military Cognitive Psychology School of PsychologyArmy Medical University(Third Military Medical University)ChongqingChina.

Focal and systemic infections are serious threats to human *** models enable the development of new drugs and therapeutic *** vivo,animal bioluminescence(BL)imaging has been used with bacterial reporter strains to evaluate antimicrobial treatment ***,high-sensitivity bioluminescent systems are required because of the limited tissue penetration and low brightness of the BL signals of existing ***,we report that NanoLuc(Nluc)showed better performance than LuxCDABE in ***,the retention rate of plasmid constructs in bacteria was *** construct stable Staphylococcus aureus reporter strains,a partner protein enolase(Eno)was identified by screening of *** strain USA300 for fusion expression of Nluc-based luciferases,including Nluc,Teluc,and *** substrates,such as hydrofurimazine(HFZ),furimazine(FUR),and diphenylterazine(DTZ),were used to optimize a stable reporter strain/substrate pair for BL *** USA300/Eno-Antares2/HFZ produced the highest number of photons of orange-red light in vitro and enabled sensitive BL tracking of *** in vivo,with sensitivities of approximately 10 CFU from mouse skin and 750 CFU from mouse ***300/Eno-Antares2/HFZ was a powerful combination based on the longitudinal evaluation of the therapeutic efficacy of *** optimized *** Eno-Antares2/HFZ pair provides a technological advancement for the in vivo evaluation of antimicrobial treatment.

关键词： antimicrobial evaluation bioluminescence imaging Nluc-based luciferases Staphylococcus aureus therapeutic efficacy

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明代版画名手刘次泉考论

中国典籍与文化 2022年第4期 145-153页

作者：胡小梅福建江夏学院设计与创意学院

对刘次泉的研究,前人集中关注其以刻工身份参与的版刻活动。实际上除镌图之外,刘次泉还善于绘图,并曾以刊行者身份参与刻书。现存由刘次泉镌图或绘图的刊本超过20种,以小说、戏曲等通俗文学为主,其中近三分之一为《三国志演义》。其创... 详细信息

对刘次泉的研究,前人集中关注其以刻工身份参与的版刻活动。实际上除镌图之外,刘次泉还善于绘图,并曾以刊行者身份参与刻书。现存由刘次泉镌图或绘图的刊本超过20种,以小说、戏曲等通俗文学为主,其中近三分之一为《三国志演义》。其创作时间主要在万历年间至崇祯末年,大部分时间服务于建阳书坊,曾短暂寓居杭州等地。刘次泉的版画创作体现出较强的变革意识。

关键词：刘次泉版画创作建阳刻书三国志演义