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DKK3调控羊驼黑色素细胞中黑色素生成

中国生物化学与分子生物学报 2021年第2期37卷 214-221页

作者：焦丁兴贾琼胡世雄杨婉赟范瑞文耿建军山西农业大学动物医学学院羊驼生物工程实验室山西太谷030801

Dickkopf-3(DKK3),Wnt/β-catenin信号通路中一个重要的抑制因子,可能参与调控黑色素生成过程。本文研究了DKK3在羊驼黑色素细胞中黑色素生成的作用。在羊驼黑色素细胞中,过表达DKK3显著下调Wnt1,Lef1,Myc和黑色素生成相关基因MITF及其... 详细信息

Dickkopf-3(DKK3),Wnt/β-catenin信号通路中一个重要的抑制因子,可能参与调控黑色素生成过程。本文研究了DKK3在羊驼黑色素细胞中黑色素生成的作用。在羊驼黑色素细胞中,过表达DKK3显著下调Wnt1,Lef1,Myc和黑色素生成相关基因MITF及其下游基因TYR,TYRP1和TYRP2的表达,在mRNA和蛋白质水平均明显下降(P<0.05);总黑素,褐黑素和真黑素的含量分别下降80.30%、72.17%和64.60%(P<0.05)。相反,在羊驼黑色素细胞中转染siRNA-DKK3,一种小干扰RNA,可以显著上调Wnt1,Lef1,Myc和黑色素生成相关基因MITF及其下游基因TYR,TYRP1和TYRP2在mRNA和蛋白质水平的表达(P<0.05);总黑素、褐黑素和真黑素的含量分别增加1.65倍、1.25倍和1.21倍(P<0.05)。这些结果表明,DKK3可以通过Wnt/β-catenin信号通路介导MITF下调羊驼黑色素细胞中黑色素的生成。

关键词： Dickkopf-3(DKK3) 黑色素生成无翅型MMTV整合位点家族成员1 小眼畸形相关转录因子黑色素细胞

丝裂原活化蛋白激酶15纳米抗体的制备及其在B16-F10黑素瘤细胞生长过程中的抑制作用

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中国生物化学与分子生物学报 2024年第1期40卷 72-80页

作者：贾琼金聪俐胡世雄秦蓉芬赵立峰范瑞文山西农业大学动物医学学院山西晋中030801 天津市北辰区农业发展服务中心天津300400

丝裂原活化蛋白激酶15(mitogen-activated protein kinase 15,MAPK15),又称ERK7或ERK8,是MAPK家族的非典型新成员。MAPK15不同程度地促进不同肿瘤细胞的增殖、迁移、自噬等细胞活动。本研究以MAPK15为靶点,筛选特异性的MAPK15纳米抗体,... 详细信息

丝裂原活化蛋白激酶15(mitogen-activated protein kinase 15,MAPK15),又称ERK7或ERK8,是MAPK家族的非典型新成员。MAPK15不同程度地促进不同肿瘤细胞的增殖、迁移、自噬等细胞活动。本研究以MAPK15为靶点,筛选特异性的MAPK15纳米抗体,评估其是否能够作为免疫组织化学和Western印迹中的一抗用于其抗原表达的检测,并探究该纳米抗体在B16-F10黑色素瘤细胞中的作用。通过噬菌体展示技术从B16-F10黑色素瘤细胞纳米抗体文库中进行筛选,得到1株MAPK15特异性纳米抗体,命名为MAPK15-VHH;将该菌株构建原核表达载体,进行优化诱导表达条件时发现,0.6 nmol/L IPTG,15℃,100 r/min条件下该纳米抗体的上清表达量最高。通过竞争ELISA法检测MAPK15-VHH的亲和力,结果显示,该抗体KD值为0.9829。通过Western印迹和免疫组织化学检测脑组织中MAPK15在蛋白质水平的表达量及分布情况,结果表明,MAPK15-VHH可与组织中的MAPK15结合,用于检测MAPK15蛋白的表达情况。在B16-F10黑色素瘤细胞中过表达MAPK15后向其中添加MAPK15-VHH,通过CCK8、Western印迹以及实时荧光定量PCR检测其对该细胞增殖和自噬的影响,结果显示,该MAPK15-VHH能作为MAPK15的拮抗剂,抑制B16-F10细胞的增殖和自噬,从而抑制黑素瘤细胞的生长。综上所述,本研究成功得到一株亲和力较强的MAPK15纳米抗体,可用于Western印迹及免疫组织化学以检测MAPK15在蛋白质水平的表达及分布;此外,该MAPK15纳米抗体可以作为MAPK15拮抗剂,有效地抑制B16-F10细胞的增殖和自噬进程,为临床检测试剂和治疗药物的开发提供理论基础。

关键词：丝裂原活化蛋白激酶15 纳米抗体黑色素瘤细胞增殖自噬

A five-compartment model of age-specific transmissibility of SARS-CoV-2

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Infectious Diseases of Poverty 2020年第4期9卷 35-49页

作者： Ze-Yu Zhao Yuan-Zhao Zhu Jing-Wen Xu shi-xiong hu Qing-Qing hu Zhao Lei Jia Rui Xing-Chun Liu Yao Wang Meng Yang Li Luo Shan-Shan Yu Jia Li Ruo-Yun Liu Fang Xie Ying-Ying Su Yi-Chen Chiang Ben-hua Zhao Jing-An Cui Ling Yin Yan-hua Su Qing-Long Zhao Li-Dong Gao Tian-Mu Chen State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics School of Public HealthXiamen University4221-117 South Xiang'an RoadXiang'an DistrictXiamen City 361102Fujian ProvincePeople's Republic of China hunan Provincial Center for Disease Control and Prevention 405 Furong Middle Road Section OneKaifu DistrictChangsha City 410001Hunan ProvincePeople's Republic of China Division of Public Health School of MedicineUniversity of Utah201 Presidents CircleSalt Lake CityUT 84112USA Department of Mathematics School of ScienceBeijing University of Civil Engineering and ArchitectureBeijingPeople's Republic of China Shenzhen Institutes of Advanced Technology Chinese Academy of SciencesShenzhenGuangdong ProvincePeople's Republic of China Jilin Provincial Center for Disease Control and Prevention 3145 Jingyang Big RoadLvyuan DistrictChangchun 130062Jilin ProvincePeople's Republic of China

Background:The novel coronavirus,severe acute respiratory syndrome coronavirus 2(SARS-CoV-2,also called 2019-nCoV)causes different morbidity risks to individuals in different age *** study attempts to quantify the age-specific transmissibility using a mathematical ***:An epidemiological model with five compartments(susceptible-exposed-symptomatic-asymptomatic-recovered/removed[SEIAR])was developed based on observed transmission *** disease 2019(COVID-19)cases were divided into four age groups:group 1,those≤14years old;group 2,those 15 to 44years old;group 3,those 45 to 64years old;and group 4,those≥65 years *** model was initially based on cases(including imported cases and secondary cases)collected in hunan Province from January 5 to February 19,*** dataset,from Jilin Province,was used to test the ***:The age-specific SEIAR model fitted the data well in each age group(Phunan Province,the highest transmissibility was from age group 4 to 3(median:β43=7.71×10-9;SAR43=3.86×10-8),followed by group 3 to 4(median:β34=3.07×10-9;SAR34=1.53×10-8),group 2 to 2(median:β22=1.24×10-9;SAR22=6.21×10-9),and group 3 to 1(median:β31=4.10×10-10;SAR31=2.08×10-9).The lowest transmissibility was from age group 3 to 3(median:β33=1.64×10-19;SAR33=8.19×10-19),followed by group 4 to 4(median:β44=3.66×10-17;SAR44=1.83×10-16),group 3 to 2(median:β32=1.21×10-16;SAR32=6.06×10-16),and group 1 to 4(median:β14=7.20×10-14;SAR14=3.60×10-13).In Jilin Province,the highest transmissibility occurred from age group 4 to 4(median:β43=4.27×10-8;SAR43=2.13×10-7),followed by group 3 to 4(median:β34=1.81×10-8;SAR34=9.03×10-8).Conclusions:SARS-CoV-2 exhibits high transmissibility between middle-aged(45 to 64 years old)and elderly(≥65 years old)***(≤14 years old)have very low susceptibility to *** study will improve our understanding of the transmission feature of SARS-CoV-2 in different age groups and suggest the most pre

关键词： Transmissibility SARS-CoV-2 COVID-19 Compartmental model Age-specific dynamics

AAV mediated carboxyl terminus of Hsp70 interacting protein overexpression mitigates the cognitive and pathological phenotypes of APP/PS1 mice

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Neural Regeneration Research 2025年第1期20卷 253-264页

作者： Zhengwei hu Jing Yang Shuo Zhang Mengjie Li Chunyan Zuo Chengyuan Mao Zhongxian Zhang Mibo Tang Changhe shi Yuming Xu Department of Neurology The First Affiliated Hospital of Zhengzhou UniversityZhengzhou UniversityZhengzhouHenan ProvinceChina Academy of Medical Sciences of Zhengzhou University ZhengzhouHenan ProvinceChina NHC Key Laboratory of Prevention and treatment of Cerebrovascular Diseases The First Affiliated Hospital of Zhengzhou UniversityZhengzhou UniversityZhengzhouHenan ProvinceChina Henan Key Laboratory of Cerebrovascular Diseases The First Affiliated Hospital of Zhengzhou UniversityZhengzhou UniversityZhengzhouHenan ProvinceChina Institute of Neuroscience Zhengzhou UniversityZhengzhouHenan ProvinceChina Sino-British Research Centre for Molecular Oncology National Centre for International Research in Cell and Gene TherapySchool of Basic Medical SciencesAcademy of Medical SciencesZhengzhou UniversityZhengzhouHenan ProvinceChina Department of Gerontology The First Affiliated Hospital of Zhengzhou UniversityZhengzhou UniversityZhengzhouHenan ProvinceChina

The E3 ubiquitin ligase,carboxyl terminus of heat shock protein 70(Hsp70)interacting protein(CHIP),also functions as a co-chaperone and plays a crucial role in the protein quality control *** this study,we aimed to investigate the neuroprotective effect of overexpressed CHIP on Alzheimer’s *** used an adeno-associated virus vector that can cross the blood-brain barrier to mediate CHIP overexpression in APP/PS1 mouse *** overexpression significantly ameliorated the performance of APP/PS1 mice in the Morris water maze and nest building tests,reduced amyloid-βplaques,and decreased the expression of both amyloid-βand phosphorylated *** also alleviated the concentration of microglia and astrocytes around *** APP/PS1 mice of a younger age,CHIP overexpression promoted an increase in ADAM10 expression and inhibitedβ-site APP cleaving enzyme 1,insulin degrading enzyme,and neprilysin *** of HSP70 and HSP40,which have functional relevance to CHIP,were also *** nuclei transcriptome sequencing in the hippocampus of CHIP overexpressed mice showed that the lysosomal pathway and oligodendrocyte-related biological processes were up-regulated,which may also reflect a potential mechanism for the neuroprotective effect of *** research shows that CHIP effectively reduces the behavior and pathological manifestations of APP/PS1 ***,overexpression of CHIP could be a beneficial approach for the treatment of Alzheimer’s disease.

关键词： adeno-associated virus Alzheimer’s disease APP/PS1 mice carboxyl terminus of Hsp70 interacting protein gene therapy

Direct Photolithography of WO_x Nanoparticles for High-Resolution Non-Emissive Displays

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NANO-MICRO LETTERS 2025年第1期17卷 1-13页

作者： Gu, Chang Yang, Guojian Wang, Wenxuan shi, Aiyan Fang, Wenjuan Qian, Lei hu, Xiaofei Zhang, Ting Xiang, Chaoyu Zhang, Yu-Mo Chinese Acad Sci Ningbo Inst Mat Technol & Engn Lab Optoelect Informat Technol & Devices Ningbo 315201 Peoples R China Qianwan Inst CNITECH Hangzhou Bay Lab Adv Nanooptoelectron Mat & Device Ningbo 315336 Peoples R China Zhejiang Univ Innovat Ctr Yangtze River Delta Smart Mat Architecture Res Lab Jiaxing 314100 Peoples R China Univ Sci & Technol China Hefei 230026 Peoples R China Jilin Univ Coll Chem State Key Lab Supramol Struct & Mat Changchun 130012 Peoples R China

High-resolution non-emissive displays based on electrochromic tungsten oxides (WOx) are crucial for future near-eye virtual/augmented reality interactions, given their impressive attributes such as high environmental stability, ideal outdoor readability, and low energy consumption. However, the limited intrinsic structure of inorganic materials has presented a significant challenge in achieving precise patterning/pixelation at the micron scale. Here, we successfully developed the direct photolithography for WOx nanoparticles based on in situ photo-induced ligand exchange. This strategy enabled us to achieve ultra-high resolution efficiently (line width m, the best resolution for reported inorganic electrochromic materials). Additionally, the resulting device exhibited impressive electrochromic performance, such as fast response ( 3600 cycles), as well as promising applications in electronic logos, pixelated displays, flexible electronics, etc. The success and advancements presented here are expected to inspire and accelerate research and development (R&D) in high-resolution non-emissive displays and other ultra-fine micro-electronics.

关键词： Electrochromic Direct photolithography WOx nanoparticles In situ photo-induced ligand exchange High-resolution displays

Epidemic of Rabies and Effect of Its Vaccine against a Dog That Consecutively Attacked Ten People in One Day

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Biomedical and Environmental Sciences 2014年第1期27卷 60-64页

作者： GAO Li Dong ZHANG Hong CAI Liang CHEN Bo Zhong JIANG Yong Lin LIU Yun Zhi LV Xin Jun YU Peng Cheng hu shi xiong LIU Fu Qiang LI Hao LI Ge Ying SHEN Xin Xin TAO Xiao Yan ZHANG Si Yu LIU Jia hui TANG Qing LI Jun hua hunan Center for Disease Control and Prevention Key Laboratory of Microbial Molecular Biology of Hunan Province Yongzhou Center for Disease Control and Prevention Key Laboratory for Medical Virology Ministry of HealthInstitute for Viral Disease Control and PreventionChina CDC Ningyuan Center for Disease Control and Prevention

On December 21, 20：10, a stray dog consecutively attacked 10 people in Lengshui Village, Ningyuan County, Yongzhou City, hunan Province, China. The dog was killed by the local CDC staff and vicinity villager, its brain tissue sample was taken within 24 h. The epidemic focus was disinfected and the injured people received post exposure prophylaxis （PEP）. Pathogens were detected in the tissue sample by the provincial CDC. The immunity and safety of rabies vaccine were assayed after PEP, the injured people were regularly followed up in the following 2 y and 6 mon.

关键词： Epidemic of Rabies and Effect of Its Vaccine against a Dog That Consecutively Attacked Ten People in One Day gene Figure PV PEP

LncRNA00067110通过靶向Cabyr调控B16-F10细胞增殖、凋亡和黑色素生成

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中国生物化学与分子生物学报 2022年第6期38卷 799-808页

作者：杨婉赟 Ajab Khan 贾琼胡世雄焦丁兴于雷涛范瑞文山西农业大学动物医学学院山西太谷030801 农业大学兽医和动物科学学院巴基斯坦开伯尔普赫图赫瓦省德拉伊斯梅尔汗29050

长非编码RNA(lncRNA)是一类转录长度大于200个核苷酸的非编码RNA。现已证明,多个lncRNA是潜在的癌症治疗靶点。LncRNA00067110是从小鼠黑色素瘤B16-F10细胞和正常黑色素细胞转录物组图谱中发现的差异表达基因。为研究lncRNA00067110是... 详细信息

长非编码RNA(lncRNA)是一类转录长度大于200个核苷酸的非编码RNA。现已证明,多个lncRNA是潜在的癌症治疗靶点。LncRNA00067110是从小鼠黑色素瘤B16-F10细胞和正常黑色素细胞转录物组图谱中发现的差异表达基因。为研究lncRNA00067110是否调控B16-F10细胞的增殖、凋亡和黑色素生成,本文通过LncTar预测和双荧光酶活性验证了钙结合酪氨酸磷酸化调节蛋白(Cabyr)和lncRNA00067110存在靶向关系。通过构建lncRNA00067110的过表达载体,转染B16-F10细胞,经过对B16-F10细胞的转录图谱分析,并对细胞增殖、凋亡和黑色素生成的表型以及相关基因表达变化进行了检测。结果显示,lncRNA00067110靶向Cabyr,在过表达lncRNA00067110的B16细胞中,17个基因呈差异表达。其中,Cabyr的表达被上调,细胞增殖相关基因MEK/ERK/MNK/CREB和黑色素生成相关基因TYR家族成员及CREB的mRNA和蛋白质水平显著被下调,凋亡相关基因AKT和Bcl-2的mRNA水平和蛋白质丰度被上调。进一步通过细胞增殖和凋亡的表型的变化验证了lncRNA00067110的功能。结果提示,lncRNA00067110通过靶向Cabyr,调控相关基因表达,从而抑制B16-F10细胞的增殖和黑色素生成,并诱导黑色素瘤细胞的凋亡,可能成为治疗和抑制黑色素瘤的新的靶点。

关键词： lncRNA00067110 B16-F10 增殖凋亡黑色素生成钙结合酪氨酸磷酸化调节蛋白(Cabyr)

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一起不明原因感染性腹泻疫情的病原学诊断

中华实验和临床病毒学杂志 2008年第6期22卷 461-463页

作者：刘运芝黄一伟靳淼张红刘富强王静胡世雄欧新华段招军湖南省疾病预防控制中心长沙410005 中国疾病预防控制中心病毒病预防控制所腹泻室湖南省长沙市疾病预防控制中心

目的明确一起不明原因感染性腹泻疫情的病原体及其基因型别。方法通过流行病学调查的方法，查明罹患率。对28例疑似病例的28份样本（粪便22份，呕吐物3份，肛拭子3份）采用RT-PCR方法进行诺如病毒核酸检测，并对5份阳性标本的核酸进行... 详细信息

目的明确一起不明原因感染性腹泻疫情的病原体及其基因型别。方法通过流行病学调查的方法，查明罹患率。对28例疑似病例的28份样本（粪便22份，呕吐物3份，肛拭子3份）采用RT-PCR方法进行诺如病毒核酸检测，并对5份阳性标本的核酸进行序列测定及遗传进化分析。结果在调查的5694人中，发病160例，罹患率为2．81％。2007年3月7-913为发病高峰。RT-PCR检测28份样本中，确定14份为阳性。对其中的5株毒株的核酸序列测定及序列遗传进化分析确定为GⅡ／4型诺如病毒，并与2006年诺如病毒流行株2006b同源性最高，为97．9％。结论本次感染性腹泻疫情由诺如病毒引起。

关键词：诺如病毒感染性腹泻病原学

湖南省2009～2013年细菌性痢疾流行病学特征及病原分析

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中国热带医学 2014年第8期14卷 950-952页

作者：罗垲炜夏昕胡世雄张红湖南省疾病预防控制中心湖南长沙410005

目的了解湖南省2009～2013年细菌性痢疾的流行病学特征和病原学特征,为制定防控策略提供科学依据。方法采用描述性流行病学方法对湖南省卫生机构2009～2013年通过全国疾病监测系统报告的细菌性痢疾监测数据进行描述性分析。结果 2009... 详细信息

目的了解湖南省2009～2013年细菌性痢疾的流行病学特征和病原学特征,为制定防控策略提供科学依据。方法采用描述性流行病学方法对湖南省卫生机构2009～2013年通过全国疾病监测系统报告的细菌性痢疾监测数据进行描述性分析。结果 2009～2013年湖南省共报告细菌性痢疾47 175例,年均发病率为14.47/10万,呈逐年下降趋势,发病具有明显的季节性,湘西和湘南地区是菌痢高发地区,发病以3岁以下幼儿为主,男性发病水平高于女性。暴发疫情主要发生在农村的中小学校,以水源性传播为主。2011～2013年湖南省疾控中心共收集176株菌痢菌株,其中宋内氏志贺菌109株,福氏志贺菌67株;湖南省志贺菌耐药较严重,不同群别志贺菌的耐药谱有所不同。结论湖南省细菌性痢疾疫情分布存在比较明显的高发地区、高发时间和高发人群,应根据流行特征和病原学特征采取针对性的防控措施。

关键词：细菌性痢疾流行病学病原学特征

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