检索结果-南通市图书馆

Response Surface Optimization of Nigella glandulifera Freyn Seed Oil Yield by Supercritical Carbon Dioxide Extraction

引用

Journal of Integrative Agriculture 2012年第1期11卷 151-158页

作者： ZHANG Jun-ping hou xi-lin YU Tian LI Ying DONG Hai-yan State Key Laboratory of Crop Genetics ＆ Germplasm Enhancement Nanjing Agriculture University Nanjing 210095 P.R. China Key Laboratory of Southern Vegetable Crop Genetic Improvement Ministry of Agriculture Nanjing 210095 P.R. China

Supercritical carbon dioxide （SC-CO2） extraction was employed to extract oil from Nigella glandulifera Freyn seed in this study. Response surface methodology （RSM） was applied to evaluate the effects of the process parameters （pressure, temperature, and CO2 flow rate） on oil yield of N. glandulifera seed. A Box-Behnken design was used to optimize the extraction parameters. The analysis of variance indicated that the linear coefficients of pressure and CO2 flow rate, the quadratic term coefficients of pressure and temperature and the interactions between pressure and temperature, as well as temperature and CO2 flow rate, had significant effects on the oil yield （P〈0.05）. The optimal conditions to obtain the maximum oil yield from N. glandulifera seed were pressure 30.84 MPa, temperature 40.57°C, and CO2 flow rate 22.00 L h-1. Under these optimal conditions, the yield of oil was predicted to be 38.19%. The validation experiment results agreed with the predicted values. The fatty acid composition of N. glandulifera seed oil extracted using SC-CO2 was compared with that of oil obtained by Soxhlet method. The results showed that the fatty acid compositions of oil extracted by the two methods were similar. Identification of oil compounds with gas chromatography-mass spectrometry （GC-MS） showed that the contents of unsaturated fatty acids linoleic acid （48.30%）, oleic acid （22.28%） and saturated fatty acids palmitic acid （16.65%）, stearic acid （4.17%） were the most abundant fatty acids in seed oil from N. glandulifera.

关键词： supercritical carbon dioxide extraction Nigella glandulifera Freyn seed oil response surface methodology,gas chromatography-mass spectrometry fatty acids

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Studies on Mucosal Immunity Induced by Transmissible Gastroenteritis Virus Nucleocapsid Protein Recombinant Lactobacillus casei in Mice and Sow

引用

Agricultural Sciences in China 2009年第2期8卷 231-237页

作者： WANG Gui-hua hou xi-lin YU Li-yun LIU Jian-kui WEI Chun-hua College of Life Science and Technology Heilongjiang August First Land Reclamation University Daqing 163319 P.R.China College of Animal Science and Technology Heilongjiang August First Land Reclamation University Daqing 163319 P.R.China

Mucosal immunity plays an important role in protecting pigs against transmissible gastroenteritis virus （TGEV） infection. To elicit mucosal immune response against TGEV, we developed a surface antigen display system using the poly-γ- glutamate synthetase A （pgsA） protein of Bacillus subtilis as an anchoring matrix to express recombinant fusion proteins of pgsA and nucleocapsid protein of TGEV in Lactobacillus casei. Surface location of fusion protein was verified by ELISA and indirect immunofluorescence test. Oral and intranasal inoculations of pregnant sow and mice with recombinant L. casei resulted in high levels of serum immunoglobulin G （IgG） and secretory immunoglobulin A （slgA） against recombinant N protein as demonstrated by ELISA. More importantly, the level of specific slgA in colostrum significantly increased compared with that of IgG. The serum IgG levels of the piglets increased after suckling colostrum produced by sows was previously inoculated with recombinant L. casei. These results indicate that immunization with recombinant L. casei expressing TGEV N protein on its surface elicited high levels of specific slgA and circulating IgG against TGEV N protein.

关键词： transmissible gastroenteritis viral lactic acid bacteria N protein

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Isolation and characterization of an ERF-B3 gene associated with flower abnormalities in non-heading Chinese cabbage

引用

Journal of Integrative Agriculture 2016年第3期15卷 528-536页

作者： XU Yu-chao hou xi-lin XU Wei-wei SHEN Lu-lu Lü Shan-wu ZHANG Shi-lin HU Chun-mei State Key Laboratory of Crop Genetics and Germplasm Enhancement Ministry of Science and Technology/College of Horticulture Nanjing Agricultural University Nanjing 210095 P.R. China Agriculture Committee of Feixi County Hefei 230001 P.R. China

BrcERF-B3 gene, a member of ethylene-responsive factor family, was screened from a mutant plant in non-heading Chinese cabbage（Brassica rapa ssp. chinensis） by cDNA-AFLP technology. We got full length cDNA of two BrcERF-B3 genes by homology-based cloning from two materials and found that their nucleotide sequences were the same by sequencing. The BrcERF-B3 protein, belonging to the B3 subgroup of the ERF subfamily, shared a close relationship with B. rapa. RT-PCR result showed that BrcERF-B3 expressed only in mutant stamen rather than maintainer stamen. qRT-PCR results indicated that BrcERF-B3 expressed highly during reproductive growth development and in the early of mutant buds, suggesting BrcERF-B3 might be involved in the formation of abnormal flower in mutant. What＇s more, the expression of BrcERF-B3 was more significant to ABA, Me JA and cold stresses in mutant than in maintainer and was down-regulated in NaCl treatment in two lines, implying BrcERF-B3 might be different roles in biotic and abiotic stresses.

关键词： non-heading Chinese cabbage stamen-petalody ethylene-responsive factor gene expression

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Molecular Cloning,Expression Analysis and Localization of Exo70A1 Related to Self Incompatibility in Non-Heading Chinese Cabbage(Brassica campestris ***)

引用

Journal of Integrative Agriculture 2013年第12期12卷 2149-2156页

作者： WANG Li GE Ting-ting PENG Hai-tao WANG Cheng LIU Tong-kun hou xi-lin LI Ying Horticultural Department Nanjing Agricultural University State Key Laboratory of Crop Genetics and Germplasm Enhancement Key Laboratory of Southern Vegetable Crop Genetic Improvement Ministry of Agriculture

The exocyst is a conserved protein complex,and required for vesicles tethering,fusion and polarized ***70A1,the exocyst subunit,is essential for assembly of the exocyst *** better understand potential roles of Exo70A1 in non-heading Chinese cabbage（Brassica campestris ***）,we obtained the full-length cDNA of Exo70A1 gene,which consisted of 1 917 bp and encoded a protein of 638 amino *** showed BcExo70A1 shared 94.9% identity with Brassica oleracea ***（AEI26267.1）,and clustered into a same group with other homologues in *** *** and Brassica *** localization analysis showed BcExo70A1 was localized to punctate structures in cytosol of onion epithelial *** showed that BcExo70A1 was widely presented in stamens,young stems,petals,unpollinated pistils,roots and leaves of self compatible and incompatible *** transcripts of BcExo70A1 in non- heading Chinese cabbage declined during initial 1.5 h after incompatible pollination,while an opposite trend was presented after compatible *** study reveals that BcExo70A1 could play essential roles in plant growth and development,and is related to the rejection of self pollen in non-heading Chinese cabbage.

关键词： Exo70A1 gene expression subcellular localization self-incompatibility non-heading Chinese cabbage

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Development and Characterization of Microsatellite Markers in Brassica rapa *** and Transferability Among Related Species

引用

Agricultural Sciences in China 2008年第1期7卷 19-31页

作者： CUI xiu-min DONG Yu-xiu hou xi-lin CHENG-Yan ZHANG Jing-yi JIN Min-feng National Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural University Nanjing 210095 P.R.China College of Resource and Environment Science Shandong Agricultural University Tai＇an 271018 P.R. China College of Life Science Shandong Agricultural University Tai＇an 271018 P.R.China

Simple sequence repeat （SSR） or microsatellite marker is a valuable tool for several purposes, such as mapping, fingerprinting, and breeding. In the present study, an intersimple sequence repeat （ISSR）-PCR technique was applied for developing SSR markers in non-heading Chinese cabbage （Brassica rapa）. A total of 190 SSRs were obtained. Among these, AG or CT （54.7%） was the most frequent repeat, followed by AC or GT （31.6%） of the microsatellites. The average number of the SSRs length array was 16 and 10 times, respectively. Based on the determined SSR sequences, 143 SSR primer pairs were designed to evaluate their transferabilities among the related species of Brassica. The number of alleles produced per marker averaged 2.91, and the polymorphism information content （PIC） value ranged from 0 to 0.863 with an average of 0.540. Monomorphism was observed in 16 primer pairs. The transferability percentage in CC genome was higher than in BB genome. More loci occurred in the BBCC genome. This result supported the hypothesis that BB genome was divergent from A and C genomes, and AA and CC genomes were relatively close. The polymorphic primers can be exploited for further evolution, fingerprinting, and variety identification.

关键词： Brassica rapa inter-simple sequence repeats (ISSR) microsatellite marker polymorphism transferability

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Analysis and Evaluation of Nutritional Quality in Chinese Radish (Raphanus sativus L.)

引用

Agricultural Sciences in China 2008年第7期7卷 823-830页

作者： LU Zhao-liang LIU Li-wang LI xiao-yan GONG Yi-qin hou xi-lin ZHU xian-wen YANG Jin-lan WANG Long-zhi National Key Laboratory of Crop Genetics and Germplasm Enhancement College of Horticulture Nanjing Agricultural University Nanjing 210095 P.R.China Department of Plant Sciences North Dakota State University Fargo ND 58105 USA

Radish （Raphanus sativus L.） is an important vegetable crop worldwide. High nutritional quality was critical in its genetic improvement and production. The nutritional quality of 42 Chinese radish cultivars was analyzed in this study. The contents of six nutritional facts, dry matter （DM）, crude fiber （CF）, total soluble sugar （TSS）, vitamin C （Vc）, protein, and nitrate, ranged from 29.7 to 88.2, 4.507 to 18.546, 2.233 to 15.457, 0.1416 to 0.3341, 0.34 to 1.15, and 1.81 to 5.89 g·kg^-1 fresh weight （FW）, respectively. Significant differences among the 42 radish cultivars were detected in the contents of nutritional facts. The data were subjected to cross-correlation analysis and principal component analysis （PCA）. It was found that DM content was positively correlated with the content of TSS （r=0.7104）, Vc （r=0.4011） and protein （r=0.4120）. Vitamin C （Vc） content of radish showed a positive correlation （r = 0.3300） with the protein content. According to the principal component analysis, out of the 42 radish cultivars, Nau-17, Nau-28, Nau-6, Nau-11, Nau-10, Nau-27, and Nau-31 were detected with very high scores in comprehensive evaluation. It could be concluded that abundant diversity of nutritional fact content occurred in different radish genotypes, and PCA analysis was effective for selecting radish germplasm with high quality. The results could contribute useful knowledge of nutritional quality, and provide important germplasms for the elite cultivar development and the inheritance study of nutritional facts in radish.

关键词： radish nutritional facts comprehensive evaluation correlation analysis principal component analysis (PCA)

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Defect Engineering with Rational Dopants Modulation for High‑Temperature Energy Harvesting in Lead‑Free Piezoceramics

引用

Nano-Micro Letters 2025年第3期17卷 87-101页

作者： Kaibiao xi Jianzhe Guo Mupeng Zheng Mankang Zhu Yudong hou Key Laboratory of Advanced Functional Materials Ministry of EducationCollege of Materials Science and EngineeringBeijing University of TechnologyBeijing 100124People’s Republic of China

High temperature piezoelectric energy harvester(HTPEH)is an important solution to replace chemical battery to achieve independent power supply of HT wireless ***,simultaneously excellent performances,including high figure of merit(FOM),insulation resistivity(ρ)and depolarization temperature(Td)are indispensable but hard to achieve in lead-free piezoceramics,especially operating at 250°C has not been reported ***,well-balanced performances are achieved in BiFeO3–BaTiO3 ceramics via innovative defect engineering with respect to delicate manganese *** to the synergistic effect of enhancing electrostrictive coefficient by polarization configuration optimization,regulating iron ion oxidation state by high valence manganese ion and stabilizing domain orientation by defect dipole,comprehensive excellent electrical performances(Td=340°C,ρ250°C>10^(7)Ωcm and FOM_(250°C)=4905×10^(–15)m^(2)N^(−1))are realized at the solid solubility limit of manganese *** HT-PEHs assembled using the rationally designed piezoceramic can allow for fast charging of commercial electrolytic capacitor at 250°C with high energy conversion efficiency(η=11.43%).These characteristics demonstrate that defect engineering tailored BF-BT can satisfy high-end HT-PEHs requirements,paving a new way in developing selfpowered wireless sensors working in HT environments.

关键词： Lead-free piezoceramic Defect engineering Dopants modulation High-temperature Piezoelectric energy harvester

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

铜离子印迹环氧树脂基整体柱的制备及性能

引用

光谱学与光谱分析 2008年第10期28卷 2446-2449页

作者：侯琳熙郭智勇王邃宁波市新型功能材料及制备科学国家重点实验室培育基地浙江宁波315211 宁波大学材料科学与化学工程学院浙江宁波315211

以铜离子为印迹物,二乙基三胺为固化剂,聚乙二醇(PEG-2000)为致孔剂,按一定比例与环氧树脂混合后在无扰动模式下聚合。反应结束后除去致孔剂和印迹离子成功地得到了一种铜离子印迹的大尺寸环氧树脂基聚合物整体柱。以此作为固相萃取柱,... 详细信息

以铜离子为印迹物,二乙基三胺为固化剂,聚乙二醇(PEG-2000)为致孔剂,按一定比例与环氧树脂混合后在无扰动模式下聚合。反应结束后除去致孔剂和印迹离子成功地得到了一种铜离子印迹的大尺寸环氧树脂基聚合物整体柱。以此作为固相萃取柱,结合原子吸收光谱建立了用于水溶液中铜离子高选择性的富集分离测定方法。详细研究了介质酸度、吸附容量、吸附选择性、分析精密度和准确度等内容,结果满意。

关键词：整体柱环氧树脂聚乙二醇离子印迹原子吸收光谱

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

不结球白菜种质资源的研究与利用

引用

南京农业大学学报 2012年第5期35卷 35-42页

作者：侯喜林宋小明南京农业大学作物遗传与种质创新国家重点实验室/农业部华东地区园艺作物生物学与种质创制重点实验室江苏南京210095

系统总结了南京农业大学近60年来进行不结球白菜种质资源的研究与利用的进展。从研究方法,种质资源的调查、搜集和整理,形态学的观察研究,生物学特性的研究,园艺学分类法的提出,抗性鉴定与分析,主要经济性状的遗传动态,种质资源遗传多... 详细信息

系统总结了南京农业大学近60年来进行不结球白菜种质资源的研究与利用的进展。从研究方法,种质资源的调查、搜集和整理,形态学的观察研究,生物学特性的研究,园艺学分类法的提出,抗性鉴定与分析,主要经济性状的遗传动态,种质资源遗传多样性分析与核心种质构建,种质资源的利用等方面,论述了不结球白菜种质资源研究所取得的成果,并对该项研究进行了展望。

关键词：不结球白菜种质资源研究利用展望

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Diallel Crossing Analyses of Resistance to Main Diseases in Pepper(Capsicum annuum L.)

引用

Agricultural Sciences in China 2005年第8期4卷 589-593页

作者： ZOU Xue-xiao hou xi-lin CHEN Wen-chao LIU Rong-yun ZHANG Zhu-qing MA Yan-qing DAI xiong-ze YANG Yu-hong National Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural University Nanjing 210095 P.R.China National Research and Extension Center of New Pepper Variety Technology Hunan Academy of Agricultural Sciences Changsha 410125 P.R.China Institute of Vegetable and Flowers Chinese Academy of Agricultural Sciences Beijing 100081 P.R.China

Fifteen capsicum combinations were made with 6 parents by （1/2）n（n-1） diallel crossing. Genetic parameters in the resistance to TMV, CMV, phytophthora blight, bacterial spot of these combinations were studied by Hayman. The results indicated that the resistance to TMV, CMV and bacterial spot conformed genetically to the “additive-dominant” model but the resistance to phytophthora blight did not and significant epistatic dominance effect existed in it. F1 hybrid＇s resistance to CMV was controlled by homozygous dominant gene （s）, but resistance to bacterial spot by heterozygous one （s）. There were little, or no sum of dominant effect and genomes controlling the dominant expression of F1 hybrids in its phytophthora blight resistance.

关键词： Pepper (Capsicam annuum L.) Diallel crossing Resistance to disease Genetic parameter estimate

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：