Isolation and characterization of an ERF-B3 gene associated with flower abnormalities in non-heading Chinese cabbage

作     者：XU Yu-chao HOU Xi-lin XU Wei-wei SHEN Lu-lu Lü Shan-wu ZHANG Shi-lin HU Chun-mei 

作者机构：State Key Laboratory of Crop Genetics and Germplasm Enhancement Ministry of Science and Technology/College of Horticulture Nanjing Agricultural University Nanjing 210095 P.R. China Agriculture Committee of Feixi County Hefei 230001 P.R. China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2016年第15卷第3期

页      面：528-536页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：supported by the Independent Innovation Fund for Agricultural Science and Technology of Jiangsu Province  China (CX(15)1015) 

主　　题：non heading Chinese cabbage stamen petalody ethylene responsive factor gene expression 

摘      要：BrcERF-B3 gene, a member of ethylene-responsive factor family, was screened from a mutant plant in non-heading Chinese cabbage（Brassica rapa ssp. chinensis） by cDNA-AFLP technology. We got full length cDNA of two BrcERF-B3 genes by homology-based cloning from two materials and found that their nucleotide sequences were the same by sequencing. The BrcERF-B3 protein, belonging to the B3 subgroup of the ERF subfamily, shared a close relationship with B. rapa. RT-PCR result showed that BrcERF-B3 expressed only in mutant stamen rather than maintainer stamen. qRT-PCR results indicated that BrcERF-B3 expressed highly during reproductive growth development and in the early of mutant buds, suggesting BrcERF-B3 might be involved in the formation of abnormal flower in mutant. What＇s more, the expression of BrcERF-B3 was more significant to ABA, Me JA and cold stresses in mutant than in maintainer and was down-regulated in NaCl treatment in two lines, implying BrcERF-B3 might be different roles in biotic and abiotic stresses.