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Effects of Corticosterone, cAMP, cGMP, Ca^(2+), and Protein Kinase C on Apoptosis of Mouse Thymocytes Induced by X-ray Irradiation

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Biomedical and Environmental Sciences 2008年第2期21卷 167-172页

作者： SHOU-LIANG GONG LI-HUA DONG guang-WEI LIU PING-SHENG GONG WEN-TIAN LU hong-guang zhao XIAO-JING JIA YONG zhao Key Laboralory of Radiobiology Unit Ministry of Health （School of Public Health Jilin University）Changchun 130021 Jilin China Transplantation Biology Research Division State Key Laboratory of Membrane and Membrane Biotechnology Institute of Zoology Chinese Academy of SciencesBeijing 100080 China Key Laboratory of Molecular Enzymology and Engineering Ministry of Education Jinlin University Changchun 130023 Jilin China

Objective To observe the effects of signal factors of corticosterone （CS）, cAMP, cGMP, Ca^2＋ and protein kinase C （PKC） on lymphocyte apoptosis in mouse thymus induced by X-rays of 4 Gy in vitro. Methods The DNA lyric rate for thymocytes was measured by fluomspectrophotometry. Results The DNA lyric rate for thymocytes 4-8 hours after irradiation with 2-8 Gy was significantly higher than that in the control （P〈0.01）. As compared with the control, the DNA lyric rate for thymocytes treated with 0.01 μnol/L CS （P〈0.01）, 50 ng/mL cAMP （P〈0.01）, 0.05-0.4 μg/mL ionomycin （Iono, P〈0.05 or P〈0.01） or 0.05-0.4 ng/mL phorbol myristate acetate （PMA, P〈0.05 or P〈0.01）, respectively, was significantly increased, while the rate for thymocytes treated with 50 ng/mL cGMP was not significantly increased. The DNA lyric rate for thymocytes treated with 0.01 μmol/L CS （P〈0.01）, 50 ng/mL cAMP （P〈0.01）, 0.2 and 0.4 μg/mL Iono （P〈0.05）, and 0.2 and 0.4 ng/mL PMA （P〈0.05） plus 4-Gy irradiation, respectively, was significantly higher than that treated with single 4-Gy irradiation, while the rate for thymocytes treated with 50 ng/mL cGMP plus 4-Gy irradiation was not increased. When both 0.4 ***/mL Iono and 0.4 ng/mL PMA acted on the thymocytes, the DNA lyric rate for thymocytes was significantly higher than that in the control （P〈0.01）, the DNA lytic rate for thymocytes treated with both 0.4 μg/mL Iono and 0.4 ng/mL PMA plus 4-Gy irradiation was significantly higher than that treated with single 4-Gy irradiation （P〈0.05）, but was Iono plus 4-Gy irradiation or 0.4 ng/mL PMA plus 4-Gy irradiation. can promote thymocyte apoptosis induced by larger dose X-rays. not significantly higher than that treated with 0.4 μg/mL Conclusion CS, cAMP, Ca^2＋, and PKC signal factors can promote thymocyte apoptosis induced by larger dose X-rays.

关键词： Corticosterone cAMP cGMP Ca^2+ PKC X-ray irradiation Lymphocyte Apoptosis

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Increased Levels of p53 and PARP-1 in EL-4 Cells Probably Related with the Immune Adaptive Response Induced by Low Dose Ionizing Radiation in vitro

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Biomedical and Environmental Sciences 2010年第6期23卷 487-495页

作者： guang-HUI CHENG NING WU DE-Fu JIANG hong-guang zhao QIAN ZHANG JIAN-FENG WANG SHou-LIANG GONG Department of Radiotherapy China-Japan Union Hospital Jilin University Changchun 130033 Jilin China Key Laboratory of Radiobiology Ministry of Health (School of Public Health Jilin University) Changchun 130021 Jilin China Department of Nuclear Medicine the First Hospital Jilin University Changchun 130021 Jilin China

Objective This paper is to explore the DNA repair mechanism of immune adaptive response （AR） induced by low dose radiation （LDR）, the changes of mRNA levels and protein expressions of p53, ATM, DNA-PK catalytic subunit （DNA-PKcs） and PARP-1 genes in the LDR-induced AR in EL-4 cells. Methods The apoptosis and cell cycle progression of EL-4 cells were detected by flow cytometry in 12 h after the cells received the pre-exposure of 0.075 Gy X-rays （inductive dose, D 1） and the succeeding high dose irradiation （challenge dose, D2; 1.0, 1.5, and 2.0 Gy X-rays, respectively） with or without wortmannin （inhibitor of ATM and DNA-PK） and 3-aminobenzamid （inhibitor of PARP-1）. And the protein expressions and mRNA levels related to these genes were detected with flow cytometry and reverse transcription-polymerase chain reaction in 12 h after irradiation with D2. Results The mRNA and protein expressions of p53 and PARP-1 in EL-4 cells in the D1 ＋ D2 groups were much lower than those in the D2 groups, and those of PARP-1 in the 3-AB ＋ D2 and the 3-AB ＋ D1 ＋ D2 groups were much lower than those in the D2 and the D1 ＋ D2 groups. The percentage of apoptotic EL-4 cells in the 3-AB ＋ D1 ＋ D2 groups was much higher than that in the D1 ＋ D2 groups, that in the G0/G1 and the G2 ＋ M phases was much higher, and that in the S phase were much lower. Although the ATM and DNA-PKcs mRNA and protein expressions in wortmannin ＋ D1 ＋ D2 groups were much lower than those in the D1 ＋ D2 groups, there were no significant changes in the apoptosis and cell cycle progression between the wortmannin ＋ D1 ＋ D2 and the D1 ＋ D2 groups. Conclusion PARP-1 and p53 might play important roles in AR induced by LDR.

关键词： Low dose radiation (LDR) Adaptive response (AR) DNA repair

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Techniques of copper recovery from Mexican copper oxide ore

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Mining Science and Technology 2009年第1期19卷 45-48页

作者： CAO Zhan-fang Zhong hong LIU guang-yi zhao Shu-juan Institute of Chemistry and Chemical Engineering Central South University Changsha Hunan 410083 China

Mexican copper ore is a mixed ore containing mainly copper oxide and some copper sulfide that responds well to flotation. The joint techniques of flotation and leaching were studied. The results indicate that an ore containing 19.01% copper could be obtained at a recovery ratio of 35.02% by using sodium sulfide and butyl xanthate flotation. Over 83.33% of the copper oxide can be recovered from the railings by leaching in suitable conditions, such as 1 h stirring at a temperature around 25 ℃with a mixing speed of S00 r/min, an H2SO4 concentration of 1.0 mol/L and a mass ratio of the ore-slurry-liquid to solid （mL/ms） of 3. The overall yield of refined ore after flotation and leaching is over 89.18% of the copper, which is much better than sole flotation or leaching. A copper product containing more than 99.9% copper was obtained by using the process： flotation-agitation leaching- solvent extraction-electro-winning.

关键词： copper oxide ore flotation stirring leaching extraction

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Femoral epithelioid hemangioendothelioma detected with magnetic resonance imaging and positron emission tomography/computed tomography:A case report

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World Journal of Clinical Cases 2021年第6期9卷 1353-1358页

作者： hong-guang zhao Ke-Wei Zhang Sen Hou Yu-Yin Dai Song-Bai Xu Department of Nuclear Medicine The First Hospital of Jilin UniversityChangchun 130021Jilin ProvinceChina Department of Thoracic Surgery The First Hospital of Jilin UniversityChangchun 130021Jilin ProvinceChina Department of Neurosurgery The First Hospital of Jilin UniversityChangchun 130021Jilin ProvinceChina

BACKGROUND Epithelioid hemangioendothelioma(EHE)is an uncommon low-grade aggressive vascular *** can occur in almost all locations,but is rarely encountered in *** SUMMARY We report a 23-year-old man who presented with left hip pain with no obvious cause.X-ray revealed bone destruction in the left femoral neck with sclerosis at the edges of the *** resonance imaging(MRI)showed bone destruction in the medullary cavity of the left femoral head and neck.18Fdeoxyglucose-positron emission tomography/computed tomography(PET/CT)imaging showed bone destruction in the left ischium,acetabulum,and left femoral head neck,accompanied by increased radioactive uptake;the maximum standard uptake value was *** examination revealed spindleshaped mesenchymal tissue hyperplasia with scattered epithelioid *** patient underwent left femoral head replacement *** signs of recurrence were observed as of the 18-mo *** The definitive diagnosis of femoral EHE can be established aided by the MRI and PET/CT findings.

关键词： Bone radiography Hemangioendothelioma Epithelioid Vascular tumor Pathology Case report

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Suitable soil moisture contents for water use efficiency and saponins accumulation in Panax notoginseng

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Chinese Herbal Medicines 2021年第2期13卷 267-273页

作者： Yu-jie Zheng Peng-guo Xia hong-guang zhao Jian-fen Zheng Wei-guo Chai Zong-suo Liang Kai-jing Yan Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province College of Life Sciences and MedicineZhejiang Sci-Tech UniversityHangzhou 310018China Institute of Biotechnology Hangzhou Academy of Agricultural SciencesHangzhou 310024China Yunnan TASLY Notoginseng Pharmaceutical Co. LtdWenshan 663000China

Objective:The moisture content in the soil directly affects the yield and quality of Panax notoginseng,especially at the age of three years ***,the suitable moisture for the growth of *** is *** this study,the effects of different soil moisture on the growth of *** were ***:Four different water treatments(0.45 field capacity(FC),0.60 FC, 0.70 FC, and 0.85 FC) were set up in Shilin County,Yunnan Province,*** water consumption and daily dynamic of water consumption were determined daily(from April 21 to October 18,2012),and the daily dynamic of water consumption under different weather conditions(sunny and rainy) was *** transpiration coefficient and water use efficiency were calculated through dry matter accumulation and total water *** of saponins of roots of *** were analyzed by HPLC after treated,and the soil moisture content suitable for the growth of *** was estimated by regression fitting of the active ingredient accumulation and the soil moisture ***:The water consumption of 0.85 FC,0.70 FC,0.60 FC and 0.45 FC were 2.89,3.68,3.37 and 2.73 kg/plant per day,*** water consumption of *** from June to August was greater than other *** daily dynamic of water consumption on sunny days and sunny days after rain showed a "double peak" feature,and it showed a "single peak" feature on rainy *** water uses efficiency(WUE) of 0.85 FC,0.70 FC,0.60 FC and 0.45 FC were 2.51,3.32,4.59,3.39 gDW/kg H_(2)O,*** increase of soil moistu re content would reduce the WUE of *** *** the increase of soil water content,the content of notoginsenoside R_(1) and ginsenoside Rg_(1) did not change significantly,while the content of ginsenoside Rb_(1) and Rd showed a decreasing ***:Soil moisture content significantly affected the water consumption of ***,and when it was 56.4% of the maximum water h

关键词： Panax notoginseng(Burk.)F.H.Chen saponins transpiration coefficient water consumption dynamics water use efficiency

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老年心脏性猝死的临床分析

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中国危重病急救医学 2000年第11期12卷 675-676页

作者：谢英洪昭光首都医科大学附属安贞医院老年心内科北京100029

目的 :分析老年心脏性猝死 (SCD)的临床特点。方法 :回顾 37例 6 1～ 84岁 SCD者的临床资料 ,其中部分患者曾行冠状动脉造影或尸检。结果 :老年 SCD者中 81.1%生前有冠心病 ,5 6 .8%患冠心病和高血压 2种疾病。冠状动脉造影或尸检可见... 详细信息

目的 :分析老年心脏性猝死 (SCD)的临床特点。方法 :回顾 37例 6 1～ 84岁 SCD者的临床资料 ,其中部分患者曾行冠状动脉造影或尸检。结果 :老年 SCD者中 81.1%生前有冠心病 ,5 6 .8%患冠心病和高血压 2种疾病。冠状动脉造影或尸检可见冠状动脉血管粥样硬化病变广泛、严重。男性和伴有左室功能不全者 SCD的危险增高。结论 :为减少老年冠心病和高血压患者发生 SCD,临床需采取积极措施 ,改善患者心肌缺血。

关键词：心脏性猝死老年人临床特点

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SMOKING PREVALENCE IN CHINESE AGED 15 AND ABOVE

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中华医学杂志(英文版) 1987年第11期100卷 892-892页

作者： Weng Xin-zhi hong zhao-guang Chen Dan-yang National Survey Group for Prevalence of Tobacco Smoking Beijing Heart Lung and Blood Vessel Medical Center Beijing

A nation-wide survey on smoking was carried out from April to November 1984, covering all 29 provinces, autonomous regions and municipalities directly under the Central Government (except Taiwan). Stratified random sampling was done in the general population in those aged 15 years and above. A total of 519600 persons were surveyed, including 258 422 males and 261 178 females. It was shown that the average smoking rate of Chinese is 33.88%, with 61.01% males and 7.04 females. The heaviest male smokers are workers and peasants, and the females ones are professionals and workers. The highest educated people have the lowest smoking rate in both sexes. Of 343 563 non-smokers interviewed, 39.75% are passive smokers. The quit smoking rate is only 4.17% in males and 9.73% in females.

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急性心肌梗死发病至就诊时间与预后的关系

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中华心血管病杂志 2007年第1期35卷 40-43页

作者：郭路芬彭亚光李庆祥贺建华赵冬洪昭光首都医科大学附属北京安贞医院心肺血管疾病抢救中心 100029 首都医科大学公共卫生与家庭医学学院北京市心肺血管疾病研究所流行病研究室

目的分析580例急性心肌梗死(AMI)患者院前延误时间分布及心肌梗死后不同时间段的死亡情况。方法回顾性分析2004年3月至2006年3月北京安贞医院抢救中心急诊室和急诊重症监护室收治的 AMI 患者580例,其中男性428例,女性152例,平均年龄(60.... 详细信息

目的分析580例急性心肌梗死(AMI)患者院前延误时间分布及心肌梗死后不同时间段的死亡情况。方法回顾性分析2004年3月至2006年3月北京安贞医院抢救中心急诊室和急诊重症监护室收治的 AMI 患者580例,其中男性428例,女性152例,平均年龄(60.7±12.9)岁。将记录的就诊时间与发病时间的时间差作为院前延误时间(PDT)。根据 PDT 不同分为7个时间段:即1～30 min,31～60 min,61～120 min,121～240 min,241～360 min,361～720 min,>720 min。统计PDT 分布,计算各时间段心室颤动(室颤)发生率、复苏成功率、病死率,并对不同时间段死亡原因相关因素进行分析。结果 580例 AMI 患者 PDT 中位数时间为130 min。接受溶栓治疗122例(21.0%),急诊 PCI 及冠状动脉搭桥术(CABG)266例(45.9%),其他药物对症治疗192例(33.1%);比较三组 PDT,溶栓组[(104.5±2.3)min]和PCI/CABG组[(119.1±2.3)min]均低于其他药物治疗组[(290.9±3.4)min,P720 min 3.4%(2/59);复苏成功率71.7%(33/46),总病死率5.3%。年龄(OR=1.047,P=0.004)、糖尿病(OR=2.159,P=0.02)和 PDT(OR=2.159,JP=0.023)是影响患者预后的独立相关因素。结论缩短 PDT,及早进入治疗程序,挽救濒死心肌,对预防室颤、猝死发生,降低病死率至关重要。

关键词：心肌梗塞死亡原因心室颤动猝死,心脏

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冠心病患者血脂治疗间隙的调查研究

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中华老年心脑血管病杂志 2000年第4期2卷 237-239页

作者：谢英洪昭光李曦北京安贞医院老年心脏内科北京100029

目的　通过冠心病 (CHD)患者血脂治疗间隙的调查研究 ,了解住院CHD患者血脂异常的治疗现状。方法　在全国 6个城市 2 2家三级甲等医院回顾性调查住院的CHD患者病历 2 2 0 0份 ,记录患者入院 48h内的血清总胆固醇(TC)和低密度脂蛋白胆固... 详细信息

目的　通过冠心病 (CHD)患者血脂治疗间隙的调查研究 ,了解住院CHD患者血脂异常的治疗现状。方法　在全国 6个城市 2 2家三级甲等医院回顾性调查住院的CHD患者病历 2 2 0 0份 ,记录患者入院 48h内的血清总胆固醇(TC)和低密度脂蛋白胆固醇 (LDL C)检查结果 ,以及住院期间血脂异常的治疗情况。结果　依照中国《血脂异常防治建议》和美国国家胆固醇教育计划 (第二次成人治疗方案 ) ,按CHD患者应开始药物治疗标准 ,即TC≥ 5 .2 0mmol L和 (或 )LDL C≥ 3.12mmol L ,有 35 .9%的患者未接受降血脂药物治疗 ;按治疗目标标准 (TC <4.6 8mmol L ,LDL C <2 .6 0mmol L) ,有 43.1%的患者未接受降血脂药物治疗。结论　住院CHD患者的血脂异常治疗存在较大的治疗间隙 ,其造成原因是多方面的。应强化降血脂治疗意识 ,采取各种积极手段 ,使CHD患者得到充分、合理的降血脂治疗 ,减少冠状动脉疾病的复发 ,提高CHD的二级预防水平。

关键词：冠状动脉疾病高胆固醇血症治疗学

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贫困地区农村籍大学生返乡就业研究——以滇东北Y县为例

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闽西职业技术学院学报 2016年第3期18卷 64-68页

作者：张光红赵欢贵州大学贵阳550025

基于2015年对滇东北Y县多个乡村社会的田野调查,以农村大学生就业为材料,对贫困地区农村籍大学生返乡就业现象展开了研究。乡村传统文化和现有生存环境及以情感为主体的社会结构严重制约了农村籍大学生返乡就业。然而,随着贫困地区农村... 详细信息

基于2015年对滇东北Y县多个乡村社会的田野调查,以农村大学生就业为材料,对贫困地区农村籍大学生返乡就业现象展开了研究。乡村传统文化和现有生存环境及以情感为主体的社会结构严重制约了农村籍大学生返乡就业。然而,随着贫困地区农村社会发展,乡村社会在市场价值体系进入后将对农村传统价值观产生冲击,农村传统关系将会被理性关系所取代,农村大学生返乡就业将成为不可阻挡的趋势,但这一过程将是缓慢而长久的。

关键词：贫困地区农村籍大学生就业研究

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